Differential Quantitative Requirements for NPR1 Between Basal Immunity and Systemic Acquired Resistance in Arabidopsis thaliana

Non-expressor of pathogenesis-related (PR) genes1 (NPR1) is a key transcription coactivator of plant basal immunity and systemic acquired resistance (SAR). Two mutant alleles, npr1-1 and npr1-3, have been extensively used for dissecting the role of NPR1 in various signaling pathways. However, it is unknown whether npr1-1 and npr1-3 are null mutants. Moreover, the NPR1 transcript levels are induced two- to threefold upon pathogen infection or salicylic acid (SA) treatment, but the biological relevance of the induction is unclear. Here, we used molecular and biochemical approaches including quantitative PCR, immunoblot analysis, site-directed mutagenesis, and CRISPR/Cas9-mediated gene editing to address these questions. We show that npr1-3 is a potential null mutant, whereas npr1-1 is not. We also demonstrated that a truncated npr1 protein longer than the hypothesized npr1-3 protein is not active in SA signaling. Furthermore, we revealed that TGACG-binding (TGA) factors are required for NPR1 induction, but the reverse TGA box in the 5’UTR of NPR1 is dispensable for the induction. Finally, we show that full induction of NPR1 is required for basal immunity, but not for SAR, whereas sufficient basal transcription is essential for full-scale establishment of SAR. Our results indicate that induced transcript accumulation may be differentially required for different functions of a specific gene. Moreover, as npr1-1 is not a null mutant, we recommend that future research should use npr1-3 and potential null T-DNA insertion mutants for dissecting NPR1’s function in various physiopathological processes.

In Arabidopsis, the NPR1 transcripts accumulate constitutively at a low basal level throughout the plant, and the accumulation level can be induced two-to threefold upon pathogen infection or SA treatment (Cao et al., 1997;Ryals et al., 1997). In the 5' untranslated region (5'UTR) of NPR1, there are three W-box (TTGAC) sequences within a 28-bp region from position 103 to 129 upstream of the translation start site (Yu et al., 2001). The third reverse W box overlaps with a TGA-box (TGACG) sequence that is recognized by TGA transcription factors (Thibaud-Nissen et al., 2006). The two adjacent W boxes have been shown to be required for NPR1 gene induction, but the function of the third W box-TGA box overlapping site is unclear (Yu et al., 2001). Similarly, while W box-binding WRKY transcription factors have been shown to regulate NPR1 transcription (Yu et al., 2001;Chai et al., 2014), whether TGA factors also participate in the regulation is unknown.
In npr1 mutants, basal transcript levels of the npr1 gene are similar to those of the wild type, but SA-and pathogen-mediated induction of the gene is compromised (Ryals et al., 1997;Kinkema et al., 2000;Zhang et al., 2012). These results indicate that NPR1 is required for induction but not for basal transcription of its own gene. Although previous work suggested that basal NPR1 transcript levels might be sufficient for SAR (van Wees et al., 2000), basal and induced transcript levels of NPR1 have never been separately evaluated when characterizing NPR1's function. It remains unknown whether basal NPR1 and induced NPR1 play different functions in some of the signaling processes in which NPR1 is involved.
Here, we show that npr1-3 is a potential null mutant, whereas npr1-1 accumulates a low level of mutant protein and should not be considered null. We demonstrated that a truncated npr1 protein longer than the putative npr1-3 protein is not active in SA signaling. Furthermore, we confirmed that NPR1 autoregulates its own gene induction (Ryals et al., 1997;Kinkema et al., 2000;Zhang et al., 2012;Chen et al., 2019), and revealed that TGA factors are required for NPR1 induction, but the TGA box (the W box-TGA box overlapping site) in the 5'UTR of NPR1 is dispensable for the induction. Finally, our results show that full induction of NPR1 is required for basal immunity, but not for SAR, whereas sufficient basal transcription is essential for full-scale establishment of SAR, indicating differential quantitative requirements for NPR1 in these immune responses.
Inoculation of plants with Psm ES4326 was performed by pressure-infiltration with a 1 ml needleless syringe as described previously (Clarke et al., 1998). After inoculation, eight infected leaves were collected for each genotype, treatment, or time point to determine in planta growth of the pathogen. For SAR induction, three lower leaves on each plant were inoculated with the virulent bacterial pathogen Psm ES4326 (OD 600 = 0.002). Two days later, the upper uninfected systemic leaves were either collected for gene expression analysis or challengeinoculated with Psm ES4326 (OD 600 = 0.001) for resistance test. Eight leaves were collected 3 days after challenge inoculation to examine the pathogen growth.

Plasmid Construction and Plant Transformation
Site-directed mutagenesis of the TGA box in the 5'UTR of NPR1 was performed in the previously reported NPR1pro:Myc-NPR1 construct  using a PCR-based Quick-Change site-directed mutagenesis kit (Stratagene, La Jolla, CA). The presence of the expected mutation in the resulting construct was identified by a CAPS marker and verified by DNA sequencing. For creating mutations in the TGA box through gene editing, a nuclease guide sequence (spacer) was introduced into the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) vector pHSE401 following the published method . For the ELP3pro:NPR1 construct, the ELP3 promoter was amplified from Col-0 genomic DNA, digested with HindIII and BamHI, and cloned into the corresponding sites of the T-DNA binary vector pBI101 (Clontech, Mountain View, CA). The coding region of NPR1 cDNA was then amplified, digested with BamHI and SacI, and ligated into BamHI/SacI-digested pBI101-ELP3 promoter plasmid. All primers or oligos used in this study were listed in Table S1. For plant transformation, the plasmids were introduced into the Agrobacterium strain GV3101 (pMP90) by electroporation, and transformation was performed following the floral dip method (Clough and Bent, 1998). Two independent mNPR1pro:Myc-NPR1 lines, three independent CRISPR mutants, and three independent ELP3pro:NPR1 lines were characterized in this study

Chemical Treatment
SA and b-aminobutyric acid (BABA) treatments were performed as previously described by Spoel et al. (2009) and Zimmerli et al. (2000), respectively. Briefly, plants were soil-drenched with water solutions containing indicated concentrations of sodium salicylate or BABA. Water treatments were used as the mock controls for both SA and BABA treatments.

RNA and Protein Analysis
Total RNA extraction was carried out as described by Cao et al. (1997). Reverse transcription quantitative PCR (qPCR) was performed as previously described (Defraia et al., 2013) using primers listed in Table S1. The NPR1 mRNA was detected with primers qF and qR1, NPR1 pre-mRNA was detected with qF and qR2, and Myc-NPR1 mRNA was detected with the forward primer recognizing a sequence in the Myc tag DNA and the reverse primer a sequence in the first exon of the NPR1 DNA ( Figure 1A and Table S1). Each gene expression analysis experiment was repeated three independent times. In each experiment, three independent biological samples (replicates) were collected at each time point per genotype/treatment and analyzed. Protein extraction, SDS-PAGE, and immunoblotting were performed as described previously (Mou et al., 2003). The NPR1 and NPR1-GFP proteins were detected using the anti-NPR1 antibody (Ding et al., 2016). Two batches of NPR1 antibodies were used. Both batches detected a specific NPR1 band, and the second batch also detected a non-specific band that is~6 kDa bigger than NPR1. Ponceau S staining of RuBisCo was used as the loading control. Each immunoblot analysis experiment was repeated at least three independent times, and the result from a representative experiment was presented.

Statistical Methods
Statistical analyses were performed with the data analysis tools (Student's t-test: Two Samples Assuming Unequal Variances) in Microsoft Excel of Microsoft Office 2004 for Macintosh and the one-way ANOVA in Prism 7 (GraphPad Software, La Jolla, CA).

A Truncated npr1 Protein With the N-Terminal 466 Amino Acids Is Inactive in SA Signaling
To address whether truncated npr1 proteins are functional, we tested three T-DNA insertion lines, GT_5_89558 (npr1-L) (Ding et al., 2015), SALK_204100, and SAIL_708_F09, which harbor a T-DNA insertion in the first, second, and third exons of the NPR1 gene, respectively ( Figure 1A, Table S2). These T-DNA insertion lines, together with SALK_203386, which carries a T-DNA insertion in the 5'UTR ( Figure 1A, Table S2), as well as the npr1-1, npr-2, and npr1-3 mutants, were subjected to SDS-PAGE immunoblot analysis using the previously reported anti-NPR1 antibody (Ding et al., 2016). As shown in Figure 1B, the anti-NPR1 antibody detected a major band at the expected molecular weight of 66 kDa in the wild-type ecotypes, Col-0 and Ler, but no signal was detected at the expected position in SALK_204100, GT_5_89558, and npr1-3. Furthermore, a specific band with a size smaller than that of the wild type was detected in SAIL_708_F09, and a wild-type-size band with significantly reduced intensity was detected in npr1-1, npr1-2, and SALK_203386. The anti-NPR1 antibody was developed with the N-terminal 465 amino acid residues and the npr1-3 nonsense mutation is in the codon for residue 400 (Cao et al., 1997;Ding et al., 2016). Although the epitopes recognized by the NPR1 antibody is uncertain, the antibody most likely would detect the truncated npr1-3 protein if it were expressed in the mutant plants. Thus, SALK_204100, GT_5_89558, and npr1-3 are potential null mutants, SALK_203386 is a knockdown mutant, SAIL_708_F09 is a mutant expressing a truncated npr1 protein, and npr1-1 as well as npr1-2 accumulate mutant proteins and are probably not null mutants.
The truncated npr1 protein accumulated in SAIL_708_F09 is 67 amino acids longer than the predicted npr1-3 protein. To test whether this truncated protein is functional in SA signaling, we tested its function in tolerance to SA toxicity, SA-induced PR1 gene expression, basal resistance, SAR, and crosstalk between SA and JA. As shown in Figures 1C-G, SAIL_708_F09 behaved similarly to the potential null mutants npr1-3 and SALK_204100, indicating that the truncated npr1 protein accumulated in SAIL_708_F09 is not functional in the tested SA responses.

NPR1 Autoregulates Its Own Gene Induction
To confirm the previous observations that SA-and pathogenmediated NPR1 gene induction is compromised in npr1 mutants The T-DNA insertion sites in SALK_203386, GT_5_89558 (npr1-L), SALK_204100, and SAIL_708_F09, the positions of the mutations in npr1-1, npr1-2, and npr1-3, as well as the positions of the primers used for qPCR analysis of NPR1 pre-mRNA (qF + qR1) and mature mRNA (qF + qR2) levels. The precise positions of the T-DNA insertions and mutations are shown in Table S2. (B) NPR1 protein levels in the wild-type Col-0 and Ler as well as the indicated npr1 mutant alleles. Total protein extracted from leaves of 4-week-old soil-grown plants was analyzed by reducing SDS-PAGE and immunoblotting with anti-NPR1 antibody. The arrow indicates the NPR1 band. Ponceau S staining of RuBisCo confirmed equal loading. (C) Tolerance of Col-0, npr1-3, SALK_204100, and SAIL_708_F09 seedlings to SA toxicity. Seeds were placed on ½ Murashige and Skoog (MS) agar medium containing 0.5 mM SA. After 3 days of stratification, the plate was transferred to a growth chamber and photographed 10 days later. (D) SA-induced PR1 gene expression in Col-0, npr1-3, SALK_204100, and SAIL_708-F09. Four-week-old soil-grown plants were treated with soil drenches of 1 mM SA solution (+SA) or water (-SA). Leaf tissues were collected 24 h later. Total RNA was extracted and subjected to qPCR analysis of PR1 gene expression. Expression was normalized against the constitutively expressed UBQ5. Data represent the mean of three independent samples ± standard deviation (SD). The asterisk indicates that PR1 was significantly induced in Col-0 (P < 0.01, Student's t-test). (E) Basal resistance of Col-0, npr1-3, SALK_204100, and SAIL_708-F09. Four-week-old soil-grown plants were inoculated with a low dose of Psm ES4326 (OD 600 = 0.0001). The in planta bacterial titers were determined 3 days postinoculation. Data represent the mean of eight independent samples ± SD. Different letters above the bars indicate significant differences (P < 0.05, one-way ANOVA). CFU, colony-forming units. (F) Biological induction of SAR in Col-0, npr1-3, SALK_204100, and SAIL_708-F09. Three lower leaves on each plant were inoculated with Psm ES4326 (OD 600 = 0.002) (+SAR) or mock-treated with 10 mM MgCl2 (-SAR). Two d later, two upper uninfected/untreated leaves were challenge-inoculated with Psm ES4326 (OD 600 = 0.001). The in planta bacterial titers were determined 3 days after challenge inoculation. Data represent the mean of eight independent samples ± SD. The asterisk indicates that Psm ES4326 grew significantly less in the SAR-induced plants than in the mock-treated plants (P < 0.0001, Student's t-test). (G) SA-mediated suppression of MeJA-induced PDF1.2 gene expression in Col-0, npr1-3, SALK_204100, and SAIL_708-F09. Ten-d-old seedlings grown on ½ MS medium were treated with water, 0.1 mM MeJA, or 0.1mM MeJA plus 0.5 mM SA (MeJA+SA). Total RNA was extracted from plant tissues collected 48 h after the treatment and subjected to qPCR analysis of PDF1.2 expression. Expression was normalized against the constitutively expressed UBQ5. Data represent the means of three biological replicates ± SD. Different letters above the bars indicate significant differences (P < 0.002, one-way ANOVA). The statistical comparisons were performed among genotypes for each treatment. Experiments in (B-G) were repeated three times with similar trend.  (Ryals et al., 1997;Kinkema et al., 2000;Zhang et al., 2012), we treated Col-0, npr1-1, and npr1-2 plants with SA and monitored NPR1 transcript accumulation. As shown Figure 2A, NPR1 mRNA levels increased approximately threefold 4 h after SA treatment in the Col-0 plants, but did not increase in both npr1-1 and npr1-2. To exclude the possibility that this difference was caused by instability of the npr1-1 and npr1-2 mRNA molecules, we monitored NPR1 pre-mRNA levels by qPCR analysis with the reverse primer in the first intron ( Figure 1A and Table S1). As shown in Figure 2B, after SA treatment, NPR1 pre-mRNA levels were significantly upregulated in Col-0, but not in npr1-1 and npr1-2. Consistent with the observed transcript accumulation, NPR1 protein levels were also dramatically upregulated by SA treatment in Col-0, but not in npr1-1 and npr1-2 ( Figure 2C). Furthermore, the previously reported transgenes 35Spro : NPR1-GFP and NPR1pro:Myc-NPR1 restored the SA inducibility of the endogenous npr1-2 and npr1-3 genes, respectively Zhang et al., 2012) ( Figures  2D, F). The npr1-2 protein levels appeared to be also upregulated in the 35Spro : NPR1-GFP npr1-2 transgenic plants after SA treatment ( Figure 2E), though the suspected NPR1 band could be a degradation product of NPR1-GFP. Taken together, these results confirmed that NPR1 is required for its own gene induction (Ryals

TGA Transcription Factors Are Required for NPR1 Induction
Since NPR1 interacts with a group of TGA transcription factors to regulate defense gene expression (Zhang et al., 1999;Despreś et al., 2000;Zhou et al., 2000;Subramaniam et al., 2001), we asked whether TGA factors also participate in regulating NPR1 induction.
To this end, we treated Col-0 and the previously reported tag2/3/5/ 6 quadruple mutant with SA and monitored NPR1 transcript and protein accumulation (Kesarwani et al., 2007). As shown Figures  3A, B, SA treatment significantly induced both NPR1 mature mRNA and pre-mRNA accumulation in Col-0, but not in the tag2/3/5/6 quadruple mutant. Similarly, NPR1 protein levels were dramatically increased in Col-0, but not in the quadruple mutant ( Figure 3C). These results indicate that TGA factors including TGA2, TGA3, TGA5, and TGA6 are required for SA-mediated NPR1 induction.
The TGA Box in the 5'UTR of NPR1 Is Not Required for NPR1 Induction To test whether the TGA box in the 5'UTR is required for NPR1 expression, we first made an A-to-C point mutation in the reverse TGA box to change the "CGTCA" sequence to "CGTCC" in the previously reported NPR1pro:Myc-NPR1 construct   (Figure 4A), and the resulting construct, mNPR1pro:Myc-NPR1, was transformed into the npr1-3 mutant. Two independent A B C FIGURE 3 | Evidence that TGA factors participate in regulating NPR1 gene induction. (A, B) SA-induced NPR1 mature mRNA (A) and pre-mRNA (B) accumulation in Col-0 and tga2/3/5/6. Four-week-old soil-grown plants were treated with soil drenches of 1 mM SA solution (+) or water (-). Leaf tissues were collected 4 h later. Total RNA was extracted and subjected to qPCR analysis. Expression was normalized against the constitutively expressed UBQ5. Data represent the mean of three independent samples ± SD. Different letters above the bars indicate significant differences (P < 0.03, oneway ANOVA). (C) SA-induced NPR1 protein accumulation in Col-0 and tga2/ 3/5/6. Four-week-old soil-grown plants were treated with soil drenches of 1 mM SA solution (+) or water (-). Total protein extracted from leaf tissues collected 24 h later was analyzed by reducing SDS-PAGE and immunoblotting with anti-NPR1 antibody. Ponceau S staining of RuBisCo confirmed equal loading. All experiments were repeated three times with similar trend.  single insertion homozygous mNPR1pro:Myc-NPR1 lines, 2-1 and 36-4, together with the previously generated NPR1pro:Myc-NPR1 plants were treated with SA and induction of the transgene transcript accumulation was monitored . As shown in Figure 4B, SA treatment induced Myc-NPR1 transcript accumulation to similar levels in all three transgenic lines, indicating that the point mutation introduced into the TGA box in the 5'UTR did not affect the NPR1 induction.
To confirm the result obtained with the NPR1pro:Myc-NPR1 transgene, we attempted to create mutations in the TGA box through gene editing. Fortunately, there is a PAM (protospacer adjacent motif) site, TGG, immediately downstream of the reverse TGA box (see Col-0 in Figure 4C), which allowed us to use the CRISPR/Cas9 approach to introduce mutations into the TGA box . As shown in Figure 4C, three mutant lines, 4-d, 11-T, and 11-A, were obtained. The TGA box was deleted in line 4-d, and a "T" and an "A" were inserted into the TGA box in lines 11-T and 11-A, respectively. The three CRISPR mutant lines and Col-0 plants were treated with SA and induction of the NPR1 transcript levels was monitored. As shown in Figure 4D, NPR1 mRNA levels were similarly upregulated in Col-0 and the three CRISPR mutant lines, confirming that the TGA box (the W box-TGA box overlapping site) in the 5'UTR is not required for NPR1 gene induction.

Full Induction of NPR1 Is Required for Basal Resistance but Not for BABA-Mediated Priming and Biological Induction of SAR
To test whether the T-DNA insertion in SALK_203386 affects NPR1 transcript accumulation, we treated Col-0 and SALK_203386 with SA and monitored NPR1 transcript levels. As shown in Figures 5A, B, induction of both NPR1 pre-mRNA and mature mRNA levels was significantly reduced in the SALK_203386 plants. Similarly, SA-induced NPR1 protein accumulation was also dramatically inhibited in SALK_203386 ( Figure 5C). Thus, the inducibility of the NPR1 gene is largely compromised in SALK_203386. However, SA still induced NPR1 protein accumulation in SALK_203386, which at 12 and 24 h after the treatment reached a level higher than the basal level in the Col-0 plants. The SA-induced elevation of NPR1 protein levels in SALK_203386 may be attributed to the slight, albeit not statistically significant, increase in NPR1 mRNA levels ( Figure  5B), and/or SA being able to stabilize the NPR1 protein (Fu et al., 2012;Ding et al., 2016). We found that SA-induced PR1 expression was also significantly inhibited in SALK_203386 ( Figure 5D), and that SALK_ 203386 plants were more susceptible than Col-0 to the bacterial pathogen Psm ES4326 ( Figure 5E). On the other hand, treatment with the plant defense-priming compound BABA and biological induction of SAR provided similar levels of resistance to Psm ES4326 in the Col-0 and SALK_203386 plants (Figures 5F, G). Taken together, these results indicate that the inducibility of NPR1 is important for basal resistance but not for SAR.

Sufficient Basal Transcription of NPR1 Is Necessary for Biological Induction of SAR
To evaluate the importance of basal transcription of NPR1 in SAR, we attempted to generate Arabidopsis plants with NPR1 protein levels lower than the basal level. To this end, we transformed an ELP3pro:NPR1 construct into the npr1-3 mutant. We used the ELP3 promoter, as it confers low-level constitutive gene expression (Defraia et al., 2013). NPR1 protein levels accumulated in three independent transgenic lines, 41-1, 56-5, and 60-6, treated with or without SA were lower than the basal level of NPR1 in Col-0 ( Figure 6A). We then tested whether the low levels of NPR1 in the transgenic lines are sufficient for SAR induction. As shown in Figure 6B, in none of the transgenic lines was SAR induced to the level reached in the Col-0 plants, indicating that sufficient basal transcription of NPR1 is required for full-scale induction of SAR.

DISCUSSION
The npr1-1 and npr1-3 mutant alleles have been extensively used for dissecting the signaling role of NPR1 in Arabidopsis. Glazebrook et al. (2003) reported that, in response to Psm ES4326 infection, the npr1-3 mutation affected the expression of SA-regulated genes, whereas the npr1-1 mutation affected not only SA-related genes, but also a much larger group of genes whose expression requires JA and ET signaling. Canet et al. (2012) revealed that methyl JA (MeJA)-induced resistance to the bacterial pathogen P. syringae pv. tomato (Pst) DC3000 was compromised in npr1-1, but not in npr1-3. Consistently, npr1-1 was shown to be more susceptible than npr1-3 to the fungal pathogens Vericillium longisporum and Piriformospora indica (Johansson et al., 2006;Stein et al., 2008). Furthermore, Leon-Reyes et al. (2009) indicated that SA-mediated suppression of MeJA-induced PLANT DEFENSIN1.2 (PDF1.2) expression was much less affected in npr1-3 than in npr1-1. The differences between npr1-1 and npr1-3 have been attributed to a speculated npr1-3 protein (Spoel et al., 2003;Johansson et al., 2006;Stein et al., 2008;Leon-Reyes et al., 2009). Our results indicate that npr1-3 is a potential null mutant and does not accumulate a truncated form of npr1 ( Figure 1B). In fact, a truncated NPR1 accumulated in SAIL_708_F09, which is 67 amino acids longer than the hypothesized npr1-3 protein ( Figure 1B), is not active in multiple SA responses including SA-JA crosstalk ( Figures 1C-G). Thus, the differences between npr1-1 and npr1-3 are likely caused by the npr1-1 protein ( Figure 1B), which is not active for SA signaling, but may interfere with JA and ET signaling (Canet et al., 2012). Regardless, future research should thus use npr1-3, the T-DNA insertion line SALK_204100 (Col-0 background) or GT_5_89558 (Ler background), for evaluating NPR1's function in various physiopathological processes.
NPR1 has been shown to autoregulate its own gene transcription (Ryals et al., 1997;Kinkema et al., 2000;Zhang et al., 2012;Chen et al., 2019). We show that the NPR1-interacting TGA transcription factors including TGA2, TGA3, TGA5, and TGA6 are also required for NPR1 gene induction (Figure 3). The cis-element characteristic of the TGA factor family is the TGA box that contains the core motif TGACG (Thibaud-Nissen et al., 2006). Intriguingly, mutations of the sole TGA box located in the 5'UTR of NPR1, from "TGACG" to "GGACG", "TGAACG", "TGTACG", or "CAACG", all had no effect on NPR1 gene induction (Figure 4), indicating that the TGA box in the 5'UTR is not required for NPR1 induction. A potential explanation for this discrepancy could be that TGA factors might regulate NPR1 induction by acting on an intermediate protein the binds the NPR1 promoter.
It is well known that pathogen infection induces biosynthesis of SA and expression of SAR-regulating genes including NPR1 (Durrant and Dong, 2004). van Wees et al. (2000) showed that NPR1 was not induced in the systemic (upper uninoculated) leaves three days after inoculation of the lower leaves, but the time point might be too late for detecting NPR1 induction in the systemic leaves (Ding et al., 2016). In this study, we took advantage of the T-DNA insertion line SALK_203386, in which induction of the NPR1 gene is largely compromised (Figures 5A, B), but NPR1 protein can accumulate to a level higher than the basal level in wild type after SA treatment ( Figure 5C). Results from SALK_203386 revealed that full induction of NPR1 is required for basal immunity but not for SAR ( Figures 5E, G), but did not define if an NPR1 level lower than the basal level is sufficient for SAR. To address this question, we created ELP3pro:NPR1 transgenic lines, in which NPR1 protein levels are lower than the basal level in wild type even after SA treatment ( Figure 6A). Characterization of the ELP3pro: NPR1 plants indicated that sufficient basal transcription of NPR1 is essential not only for basal immunity but also for full-scale establishment of SAR ( Figure 6B). These results, taken together, suggest differential quantitative requirements for NPR1 between  (203386). Four-week-old soil-grown plants were treated with soil drenches of 1 mM SA solution (+SA) or water (-SA). Leaf tissues were collected 4 h later. Total RNA was extracted and subjected to qPCR analysis using primer pairs qF + qR1 and qF + qR2 ( Figure 1A and Table S1) for pre-mRNA and mature mRNA, respectively. Expression was normalized against the constitutively expressed UBQ5. Data represent the mean of three independent samples ± SD. Different letters above the bars indicate significant differences (P < 0.02, one-way ANOVA). (C) SA-induced NPR1 protein accumulation in Col-0 and SALK_203386. Four-week-old soil-grown plants were treated with soil drenches of 1 mM SA solution. Total protein extracted from leaf tissues collected at the indicated time points was analyzed by reducing SDS-PAGE and immunoblotting with anti-NPR1 antibody. Ponceau S staining of RuBisCo confirmed equal loading.
(D) SA-induced PR1 gene expression in Col-0 and SALK_203386. Total RNA was extracted from leaf tissues collected 24 h after SA treatment and subjected to qPCR analysis. Expression was normalized against the constitutively expressed UBQ5. Data represent the mean of three independent samples ± SD. Different letters above the bars indicate significant differences (P < 0.001, one-way ANOVA). (E) Basal resistance of Col-0 and SALK_203386. Four-week-old soil-grown plants were inoculated with a low dose of Psm ES4326 (OD 600 = 0.0001). The in planta bacterial titers were determined 3 d postinoculation. Data represent the mean of eight independent samples ± SD. The asterisk indicates that SALK_203386 is significantly more susceptible than Col-0 to Psm ES4326 (P < 0.002, Student's t-test).
(F) BABA-induced resistance in Col-0 and SALK_203386. Four-week-old soil-grown plants were treated with soil drenches of 250 mM of BABA solution (+) or water (-). Two d later, the plants were inoculated with a high dose of Psm ES4326 (OD 600 = 0.001). The in planta bacterial titers were determined 3 d postinoculation. Data represent the mean of eight independent samples ± Different letters above the bars indicate significant differences (P < 0.05, one-way ANOVA). (G) Biological induction of SAR in Col-0 and SALK_203386. Three lower leaves on each plant were inoculated with Psm ES4326 (OD 600 = 0.002) (+SAR) or mock-treated with 10 mM MgCl 2 (-SAR). Two d later, two upper uninfected/untreated leaves were challenge-inoculated with Psm ES4326 (OD 600 = 0.001). The in planta bacterial titers were determined 3 d after challenge inoculation. Data represent the mean of eight independent samples ± SD. Different letters above the bars indicate significant differences (P < 0.002, one-way ANOVA). All experiments were repeated three times with similar trend. basal immunity and SAR in Arabidopsis. Based on our results, it can be concluded that the NPR1 threshold for full-blown basal immunity is higher than that at which SAR can be fully activated, though it is difficult to accurately determine these thresholds. Interestingly, basal levels of SA have been suggested to be sufficient for SAR induction (Chanda et al., 2011;Gao et al., 2015). It would therefore be possible that, like NPR1, basal SA and basal transcription of other SAR-regulating genes are essential for SAR and the induction is necessary for basal immunity. Further investigations are warranted to test this interesting possibility.

DATA AVAILABILITY STATEMENT
All datasets presented in this study are included in the article/ Supplementary Material.

AUTHOR CONTRIBUTIONS
YD, SD, and ZM designed the experiments. YD, MD, and CW characterized mutants. MD, QL, QZ, and XZ generated and characterized transgenic lines. YD and ZM wrote the manuscript. All authors contributed to the article and approved the submitted version.

FUNDING
This work was partially supported by a grant from the University of Florida Research Opportunity Seed Fund (grant no. PRO00018170 awarded to ZM). QZ was supported by a scholarship from the Chinese Scholarship Council.