The Photomorphogenic Transcription Factor PpHY5 Regulates Anthocyanin Accumulation in Response to UVA and UVB Irradiation

Red coloration contributes to fruit quality and is determined by anthocyanin content in peach (Prunus persica). Our previous study illustrated that anthocyanin accumulation is strongly regulated by light, and the effect of induction differs according to light quality. Here we showed that both ultraviolet-A (UVA) and ultraviolet-B (UVB) irradiation promoted anthocyanin biosynthesis in “Hujingmilu” peach fruit, and a combination of UVA and UVB had additional effects. The expression of anthocyanin biosynthesis and light signaling related genes, including transcription factor genes and light signaling elements, were induced following UV irradiation as early as 6 h post-treatment, earlier than apparent change in coloration which occurred at 72 h. To investigate the molecular mechanisms for UVA- and UVB-induced anthocyanin accumulation, the genes encoding ELONGATED HYPOCOTYL 5 (HY5), CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), Cryptochrome (CRY), and UV RESISTANCE LOCUS 8 (UVR8) in peach were isolated and characterized through functional complementation in corresponding Arabidopsis (Arabidopsis thaliana) mutants. PpHY5 and PpCOP1.1 restored hypocotyl length and anthocyanin content in Arabidopsis mutants under white light; while PpCRY1 and PpUVR8.1 restored AtHY5 expression in Arabidopsis mutants in response to UV irradiation. Arabidopsis PpHY5/hy5 transgenic lines accumulated higher amounts of anthocyanin under UV supplementation (compared with weak white light only), especially when UVA and UVB were applied together. These data indicated that PpHY5, acting as AtHY5 counterpart, was a vital regulator in UVA and UVB signaling pathway. In peach, the expression of PpHY5 was up-regulated by UVA and UVB, and PpHY5 positively regulated both its own transcription by interacting with an E-box in its own promoter, and the transcription of the downstream anthocyanin biosynthetic genes chalcone synthase 1 (PpCHS1), chalcone synthase 2 (PpCHS2), and dihydroflavonol 4-reductase (PpDFR1) as well as the transcription factor gene PpMYB10.1. In summary, functional evidence supports the role of PpHY5 in UVA and UVB light transduction pathway controlling anthocyanin biosynthesis. In peach this is via up-regulation of expression of genes encoding biosynthetic enzymes, as well as the transcription factor PpMYB10.1 and PpHY5 itself.

Red coloration contributes to fruit quality and is determined by anthocyanin content in peach (Prunus persica). Our previous study illustrated that anthocyanin accumulation is strongly regulated by light, and the effect of induction differs according to light quality. Here we showed that both ultraviolet-A (UVA) and ultraviolet-B (UVB) irradiation promoted anthocyanin biosynthesis in "Hujingmilu" peach fruit, and a combination of UVA and UVB had additional effects. The expression of anthocyanin biosynthesis and light signaling related genes, including transcription factor genes and light signaling elements, were induced following UV irradiation as early as 6 h post-treatment, earlier than apparent change in coloration which occurred at 72 h. To investigate the molecular mechanisms for UVA-and UVB-induced anthocyanin accumulation, the genes encoding ELONGATED HYPOCOTYL 5 (HY5), CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), Cryptochrome (CRY), and UV RESISTANCE LOCUS 8 (UVR8) in peach were isolated and characterized through functional complementation in corresponding Arabidopsis (Arabidopsis thaliana) mutants. PpHY5 and PpCOP1.1 restored hypocotyl length and anthocyanin content in Arabidopsis mutants under white light; while PpCRY1 and PpUVR8.1 restored AtHY5 expression in Arabidopsis mutants in response to UV irradiation. Arabidopsis PpHY5/hy5 transgenic lines accumulated higher amounts of anthocyanin under UV supplementation (compared with weak white light only), especially when UVA and UVB were applied together. These data indicated that PpHY5, acting as AtHY5 counterpart, was a vital regulator in UVA and UVB signaling pathway. In peach, the expression of PpHY5 was up-regulated by UVA and UVB, and PpHY5 positively regulated both its own transcription by interacting with an E-box in its own promoter, and the transcription of the downstream anthocyanin biosynthetic genes chalcone synthase 1 (PpCHS1), chalcone synthase 2 (PpCHS2), and dihydroflavonol 4reductase (PpDFR1) as well as the transcription factor gene PpMYB10.1. In summary,

INTRODUCTION
Anthocyanins, a group of secondary metabolites known as flavonoid compounds, are important water-soluble pigments widely distributed in plants where they perform a multitude of biological functions such as protecting against a variety of abiotic stresses [ultraviolet (UV) radiation, nutrient deficiency, low temperature, drought, etc.], attracting pollinators and seed dispersers, and defense against pathogens and herbivores (Schaefer et al., 2004;Gould et al., 2010). In addition, they are also recognized to have potential human health benefits in aspects such as prevention from cancer and heart disease (Tsuda, 2012;Zhang et al., 2015). Hence, anthocyanin is considered as an essential determinant for fruit quality.
Anthocyanin biosynthesis is not only developmentally regulated but also influenced by various environmental and endogenous stimuli as well (Guo et al., 2008). As the primary energy source and an informational signal for regulating plant growth and development, light is one of the most critical environmental factors (Kami et al., 2010). Light profoundly influences complex signaling pathways such as photomorphogenesis, circadian rhythms and the regulation of secondary metabolism throughout the life of plants (Gelderen et al., 2018).
After perceiving light, this signal is transmitted from photoreceptors to downstream light signal transduction factors to regulate the process of light-induced anthocyanin biosynthesis both transcriptionally and post-translationally (Casal, 2013). ELONGATED HYPOCOTYL 5 (HY5), a basic leucine zipper (bZIP) transcription factor, is a master regulator in light signal transduction pathway that acts downstream of multiple photoreceptors to respond to photomorphogenesis (Holm et al., 2002;Gangappa and Botto, 2016). HY5 is considered as a positive regulator to participate in light-induced anthocyanin biosynthesis in Arabidopsis, apple, grape, and pear (Loyola et al., 2016;An et al., 2017;Nawkar et al., 2017;Tao et al., 2018). AtHY5 targets the anthocyanin biosynthetic genes (CHS,F3H) and MYB [PRODUCTION OF FLAVONOL GLYCO-SIDES 1 (PFG1)/MYB12, MYB75/PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1)] to activate their expression by direct binding to the promoters (Chattopadhyay et al., 1998;Lee et al., 2007;Stracke et al., 2010;Shin et al., 2013). In eggplant (Solanum melongena) under blue light, SmHY5 has also been revealed to accelerate anthocyanin accumulation by regulating the expression of downstream genes encoding enzymes of the anthocyanin biosynthetic pathway (Jiang et al., 2016). In apple, MdHY5 promoted the expression of MdMYB10 to regulate anthocyanin accumulation . CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), an E3 ubiquitin ligase, is a crucial repressor for photomorphogenesis and anthocyanin biosynthesis, targeting HY5 for proteasomemediated degradation Lau and Deng, 2012). In pear, PpyCOP1 indirectly interacted with PpyHY5 to mediate regulation of anthocyanin accumulation (Tao et al., 2018). Additionally, apple MdCOP1 was also found to be critical for the ubiquitination and degradation of MdMYB1, a positive regulator for anthocyanin biosynthesis, further leading to the negative regulation for the coloration in apple fruit (Li et al., 2012).
Anthocyanin accumulation is essential for the quality of peach fruit. Our previous study revealed that UV light enhances anthocyanin content in peach peel (Zhao et al., 2017). However, how photoreceptors and light signal transduction elements mediate UV light-induced anthocyanin accumulation has not been elucidated. In this study, the effect of UVA and UVB irradiation on the coloration of peach fruit was examined. We cloned and functionally characterized PpHY5 and provided molecular evidence that PpHY5 plays a vital role in anthocyanin biosynthesis in response to UVA and UVB. These results contributed to our understanding of the mechanism underlying UV light-induced anthocyanin biosynthesis in peach, as well as providing evidence for improving fruit pigmentation.

Plant Materials and Growth Conditions
The peach cultivar (cv) "Hujingmilu" was used in this study. All fruit were bagged with commercial yellow paper bags at 42 days after full blossom and collected at just before turning stage. For UV irradiation treatment experiments, all collected fruit were transferred to a growth chamber at a constant 20 • C and irradiated with continuous UVA (320-400 nm, 1,000 µw/cm 2 ) or UVB (280-320 nm, 58 µw/cm 2 ) or both together for 0, 6, 12, 24, 48, 72, and 144 h, respectively. The fruit kept in the dark served as the control. Peel tissue from 30 fruit of each treatment were separated simultaneously into three biological replicates (10 fruit for each replicate) at every sampling time, then immediately frozen in liquid nitrogen and stored at −80 • C for subsequent experiments.
The Arabidopsis hy5 (SALK_056405), cop1 (SALK_022133), uvr8 (SALK_033468), and cry1 (SALK_069292) mutants with the Columbia genetic background were obtained from The Arabidopsis Information Resource (TAIR). For hypocotyl length and anthocyanin accumulation assays, seeds of wild type (WT), above-mentioned mutants as well as transgenic lines obtained in this study were sterilized and germinated on Murashige and Skoog plates supplemented with 0.6% agar and 1% sucrose, and subjected to a chilling treatment at 4 • C for 2 days in the dark. Then these seedlings were transferred to a growth chamber at 24 • C under the long-day condition (16 h photoperiod/8 h dark cycle) of white light in normal intensity (40 w/m 2 ) for growth. For UV light treatment experiment, seedlings were grown under continuous weak white light (WWL, 40 µw/cm 2 ) supplemented with UVA (40 µw/cm 2 ) or UVB (10 µw/cm 2 ) or both together at 24 • C. And the seedlings kept under only WWL served as the control. After growth under different light conditions as indicated for 5 or 7 days, respectively, the hypocotyl length was measured and seedlings were sampled for subsequent experiments.

Color Measurement
Fruit surface color measurement was carried out using a reflectance spectrophotometer (Hunter Lab Mini Scan XE Plus colorimeter) at four evenly distributed equatorial positions of per fruit. The raw data as L * (lightness, from black to white), a * (the degree of green-red variation), b * (the degree of blueyellow variation) values were recorded and the color index of red grapes (CIRG) was calculated according to the formula CIRG = (180 − H)/(L * + C), while H = arctan (b * /a * ) and C = [(a * ) 2 +(b * ) 2 ] 0.5 (Carreño et al., 1995;Zhang et al., 2008).

Anthocyanin Extraction and Measurement
Anthocyanin was extracted from peach fruit peel and Arabidopsis seedlings by using the method as described in our previous study (Zhao et al., 2017). High-performance liquid chromatography (HPLC) analysis for quantification of anthocyanin was performed using an Agilent 1260 liquid chromatograph system (Agilent Technologies, CA, United States) equipped with a ZORBAX SB-C18 column following a previously reported protocol (Cheng et al., 2014). The UV-visible light detector wavelength was set at 520 nm. Cyanidin 3-O-glucoside chloride was employed as the authentic standard.

RNA Extraction and Reverse Transcription Quantitative PCR Analysis
Total RNA was isolated from peach tissue samples and Arabidopsis by using a modified cetyltrimethylammonium bromide (CTAB) method (Liu et al., 2015) and the TRIzol Reagent Kit (Ambion, Hopkinton, MA, United States), respectively. First-strand cDNA was synthesized with HiScript R II Q Select RT SuperMix (Vazyme) after removal of genomic DNA contamination by TURBO DNase (Ambion) following the manufacturer's protocol. Reverse transcription quantitative PCR (RT-qPCR) was performed using the SsoFast EvaGreen Supermix kit (Bio-Rad, CA, United States) with the CFX96 instrument (Bio-Rad, CA, United States) according to the following program: 95 • C for 3 min, followed by 45 cycles of 95 • C for 10 s, 60 • C for 30 s and then 95 • C for 10 s followed by a continuous increase from 65 • C to 95 • C at the heating rate of 0.5 • C/s for dissociation curve analysis (Zhao et al., 2017).
The PpTEF2 (GenBank accession: No. JQ732180) and ATACT2 (GenBank accession: No. AT3G18780) genes were used as the internal reference genes to normalize expression values for peach and Arabidopsis, respectively (Tong et al., 2009;Zhou et al., 2013). The primer sequences for RT-qPCR were designed with Primer Premier 5 and listed in Supplementary Table 1.

RNA-Seq Analysis
RNA sequencing (RNA-Seq) of peach fruit samples (irradiated with different UV-light conditions for 0, 6, 12, 24, and 48 h) was conducted using the Xten platform by staff at Biomarker (Beijing, China) with three biological replicates for each sample. Data analysis was conducted as described in our previous work (Zhao et al., 2017). Transcript abundances were normalized by calculating FPKM (expected number of Fragments Per Kilobase of transcript sequence per Million base pairs sequenced).

Plasmid Construction
The full-length coding sequence of PpHY5 (Prupe.1G478400), In experiments for verifying the function of E-box motifs in PpHY5 promoter, mutations on the PpHY5 promoter were conducted using the Fast Mutagenesis System Kit (Transgene) and inserted into the pGreenII 0800-LUC vector, named PpHY5m1-LUC, PpHY5m2-LUC, PpHY5m3-LUC, and PpHY5m4-LUC, respectively. For subcellular localization analysis, the coding sequence of each gene without stop codon was fused with green fluorescent protein (GFP) and cloned into the pCAMBIA super 1300-eGFP vector (PpHY5-GFP, PpCOP1.1-GFP). All constructs were transformed into Agrobacterium tumefaciens strain GV3101 (MP90) by electroporation with GenePulser Xcell TM Electroporation Systems (Bio-Rad, Hercules, CA, United States) for subsequent experiments. Primers used for plasmid construction were described as listed in Supplementary Table 2.

Dual-Luciferase Assay
The dual-luciferase assay was performed with Nicotiana benthamiana leaves by using a previously reported protocol (Zhao et al., 2020). Agrobacterium cultures containing recombinant pGreenII 0029 62-SK vectors harboring transcription factor genes and the cultures containing recombinant pGreen II 0800-LUC vector harboring target gene promoters were infiltrated into N. benthamiana leaves with a ratio of 10:1 (v/v). At three days following infiltration, the firefly luciferase (LUC) and renilla luciferase (REN) activities were measured using dual-Luciferase Reporter Assay System (Promega, MI, United States) with Modulus Luminometer.
When the ratio of LUC/REN was over two and the difference was highly significant (P < 0.01) as compared with the control, the transcription factor was considered to be able to activate the promoter. For each transcription factor and promoter interaction, dual-luciferase assays were conducted with at least three independent experiments (six replicate reactions in each experiment).

Arabidopsis Transformation and Hypocotyl Length Measurement
The Arabidopsis transformation was performed by using the floral dip method (Zhang et al., 2006). SilwetL-77 (Real-Times, Beijing, China) was applied as a surfactant in Agrobacteriumbased transformation of Arabidopsis. Transgenic seeds were sterilized and placed on Murashige and Skoog medium containing 50 mg/mL kanamycin for transgenic plant selection. For each target gene, at least three lines were characterized and three of them with highest expression levels of target gene were selected, and individuals of the T2 were used for further phenotype analysis. After growth for 5 or 7 days, the hypocotyl length measurement of at least 30 Arabidopsis seedlings was conducted for each replicate. All experiments were carried out with three independent biological replicates.

Subcellular Localization Analysis
The Agrobacterium strain carrying the 35S::PpHY5-GFP or 35S::PpCOP1.1-GFP recombinant vector was infiltrated into transgenic N. benthamiana plant with a red fluorescent nuclear marker (Nucleus-RFP) according to a previous report (Zhao et al., 2020), while 35S::GFP was used as a negative control. The GFP florescence of the transiently expressed leave was observed under a Zeiss LSM710NLO confocal laser scanning microscope.

Cis-regulatory Element Analysis
The predictions of conserved cis-regulatory elements in the promoter were analyzed using the PlantCARE database. 1

Statistical Analysis
The statistical significance of differences was analyzed using SPSS statistical software (SPSS 19.0, SPSS Inc., Chicago, IL, United States) with Student's t-test. Graphs were plotted using Origin 8.0 (Origin Lab Corporation, Northampton, MA, United States).

Time Course of Color Development and the Expression of Anthocyanin Biosynthetic Genes Under Continuous UV Treatments
To investigate the influence of different UV light treatments on anthocyanin biosynthesis in peach, the fruit were given either a continuous UVA or UVB or UVA + UVB treatment. The control fruit was kept in the dark and did not develop red color over time. Under UV irradiation, the peel color remained green within 24 h and a few red spots began to be slightly visible at 48 h. At 72 and 144 h following irradiation, the fruit turned deep red ( Figure 1A). These observations matched well with the CIRG values ( Figure 1B). Under dark condition, the concentration of anthocyanin in peel was very low and showed no significant increase over time ( Figure 1B). In contrast, anthocyanin accumulated following irradiation with UVA or UVB or UVA + UVB, and the effects varied significantly among different UV lights conditions. The concentration of anthocyanin rose sharply to 3.12 mg/100gFW (15-fold higher than the control) under UVA exposure and 2.11 mg/100gFW (10-fold higher than the control) under UVB exposure at 144 h respectively. During the irradiation period, the highest anthocyanin content was detected in fruit under UVA + UVB treatment, reaching 3.47 mg/100gFW at 72 h (2-3 fold higher than UVA or UVB) and 11.59 mg/100gFW at 144 h (3-6 fold higher than UVA or UVB), respectively ( Figure 1B), which was consistent with the visual appearance.
In order to further explore the molecular mechanisms for anthocyanin accumulation in response to UV lights, we analyzed the transcript levels of genes encoding anthocyanin biosynthetic enzymes (PpPAL, Pp4CL, PpCHS1, PpCHS2, PpCHI, PpF3H, PpDFR1, PpDFR2, PpANS, and PpUFGT), transport genes PpGST1 and regulatory genes (PpMYB10.1, PpMYB10.2, and PpMYB10.3) in peel during the early irradiation period (0, 6, 12, 24, and 48 h) before the appearance of red coloration. Under dark condition, the assayed genes exhibited low expression levels and for some genes such as PpCHS1, PpDFR1, and PpUFGT, the transcript levels showed little change over time (Figure 2). In comparison to the low expression levels at the initial point (0 h), the expression profiles of the biosynthetic genes were up-regulated to various degrees in peel of fruit when exposed to the UV treatments. The transcripts of all assayed biosynthetic genes under different UV light conditions were elevated than control (Figure 2). After exposure to UV light for 6 h, the expression levels of all analyzed biosynthetic genes sharply increased (Figure 2). For example, the transcript levels of PpCHS1 and PpUFGT in the UV light-exposed fruit were at least 6-fold and 9-fold higher than the control. However, after exposure to UV light for 12 h, the transcript levels of PpCHI, PpF3H, PpANS, and PpGST1 decreased slightly. Pp4CL and PpCHS1 showed the highest expression after 24 h of UV light irradiation. Although there was a slight decline in the transcript abundance of some genes after irradiation for 48 h, the expression was consistently higher than the control, for instance, the transcript levels of PpDFR1 were at least 7-fold higher, 14-fold higher and 28-fold higher in the UV lightexposed fruit than that in the dark, respectively. In particular, the expression of PpGST1 continuously remained very low in the fruit under dark condition, while was strongly stimulated by UV light, showing a 43-fold, 155-fold, and 332-fold elevation at 48 h in UVA, UVB, and UVA + UVB light exposed fruit, respectively.
The expression patterns of the regulatory transcription factor genes (PpMYB10.1, PpMYB10.2, and PpMYB10.3) showed elevation by UV, but with different profiles. The transcript level of PpMYB10.1 was strongly induced by UV light and then decreased after prolonged exposure. The expression of PpMYB10.2 and PpMYB10.3 presented little change under UVA irradiation and transcript abundance was lower compared with PpMYB10.1 (Figure 2). The transcript levels of most genes were generally highest in peel of fruit irradiated with UVA + UVB, followed by UVA and UVB, while the expression were almost undetectable or showed low levels in control. Even though there were slight declines in transcription level at some time points, most genes reached the maximum transcription levels when treated with UVA + UVB for 24 h, which ensured the high transcription abundance detected in fruit at later stages. The results suggested  that the coloration of the peel was able to be induced by the irradiation of UVA or UVB, and there was a synergistic effect of UVA and UVB in stimulating anthocyanin biosynthesis.

PpCOP1.1 after UV Light Treatment
In Arabidopsis, it has been demonstrated that HY5 and COP1 play vital roles in light signal transduction pathway (Lau and Deng, 2012;Gangappa and Botto, 2016). Previously, we identified potential homologous genes in peach (named PpHY5, PpHYH, PpCOP1.1, and PpCOP1.2) (Zhao et al., 2017). To clarify their functions in UV light induced anthocyanin biosynthesis in peach fruit, the expression levels following UV light exposure were monitored.
PpHY5 expression exhibited a strong response to UV light irradiation (Figure 3). The transcript level of PpHY5 was low at the beginning (0 h); with UV there was a sharp increase which peaked within the first 6 h, and showed the highest accumulation level under UVA + UVB (about 4-fold higher than the control); from this peak there was a gradual decrease but expression remained higher than control (Figure 3). In Arabidopsis, HY5 HOMOLOG (HYH) has multiple overlapping functions with HY5 (Holm et al., 2002). In our study, the transcript level of PpHYH was similar to those of PpHY5, which increased at the beginning of irradiation but eventually decreased with continuous UV light (Figure 3). By contrast, the expression of PpCOP1.1 decreased gradually following irradiation with UVA or UVB or UVA + UVB while slightly increased in the control (Figure 3). However, PpCOP1.2 remained at quite low levels of expression with no obvious variation during the entire irradiation period among all different treatments (Figure 3). Overall, expression of PpHY5 and PpHYH increased following UV treatment while that of PpCOP1.1 was reduced. The transcript levels of these genes also responded quickly, within 6 h after the start of the treatment.

PpHY5 and PpCOP1.1 Restored Hypocotyl Length and Anthocyanin Content in Arabidopsis Mutants under White Light
To confirm the roles of PpHY5 and PpCOP1.1 as light signal transduction related genes, we conducted the functional complementation assays with the Arabidopsis hy5 mutant and cop1 mutant transformed with PpHY5 and PpCOP1.1, respectively. At least three independent transgenic lines of each background were generated and confirmed by RT-qPCR. The transgenic lines (PpHY5/hy5, PpCOP1.1/cop1) with high expression of PpHY5 or PpCOP1.1 were chosen to compare with WT and hy5 or cop1 mutant by measuring hypocotyl length and anthocyanin content under all conditions in 5-day-old seedlings.
For plants grown under white light for five days, hy5 exhibited the longest hypocotyl phenotype (11.47 mm), while cop1 exhibited the shortest hypocotyl phenotype (3.55 mm) ( Figure 4A). The constitutive overexpression of PpHY5 or PpCOP1.1 in corresponding mutant background restored the hypocotyl length under white light ( Figure 4A). The concentrations of anthocyanin in all lines also showed difference to some degrees ( Figure 4B). Compared to WT, cop1 displayed significantly higher accumulation of anthocyanin, and a lower level was observed in hy5. The anthocyanin levels were restored and enhanced in PpHY5/hy5 transgenic lines, reaching a level that was about 4.1-fold higher than that in hy5 mutant background ( Figure 4B). In comparison, the anthocyanin accumulation was inhibited in the PpCOP1.1complemented lines and decreased by 32% than those in cop1 mutant background ( Figure 4B). Likewise, the genes encoding enzymes of the anthocyanin biosynthetic pathway (PAL, CHS, CHI, F3H, DFR, ANS, and UFGT) exhibited similar transcript accumulation trends consistent with anthocyanin content ( Figure 4C). All these structural genes were generally lowly expressed in hy5 and highly expressed in cop1 relative to WT. For instance, the expression levels of ANS and UFGT decreased significantly in hy5 and were about 50 and 75% lower, whereas at least 5-fold and 4-fold higher in cop1 than that measured in WT. No significant differences in transcript levels of anthocyanin biosynthetic structural genes were found between PpHY5/hy5 or PpCOP1.1/cop1 transgenic lines and WT.
To visualize the subcellular localization of PpHY5 and PpCOP1.1, we performed the confocal microscopy assays of N. benthamiana leaves transiently transformed with PpHY5-GFP and PpCOP1.1-GFP. Our results showed that both PpHY5-GFP and PpCOP1.1-GFP displayed signal in the nucleus under white light (Supplementary Figure 1). This was in consistent with the subcellular localization of AtHY5 and AtCOP1 Osterlund et al., 2000;Lau and Deng, 2012). These data suggested that PpHY5 and PpCOP1.1 were the functional orthologous of AtHY5 and AtCOP1 and were able to participate in white-light-related photomorphogenesis.
PpHY5 Participated in UVA-and UVB-Induced Anthocyanin Accumulation in a PpCRY1 and PpUVR8.1 Dependent Manner In Arabidopsis, AtHY5 is a key factor in photomorphogenic responses and acts as a downstream signaling intermediate of AtCRY1 and AtUVR8 (Binkert et al., 2014;Marzi et al., 2020). To determine whether the peach counterpart of these genes function in the same signaling cascade in Arabidopsis, we generated the PpHY5/hy5, PpCRY1/cry1, and PpUVR8.1/uvr8 transgenic lines. Five-day-old seedlings of WT, cry1 mutant and PpCRY1/cry1 transgenic lines were exposed to WWL + UVA for periods of 0-24 h and the expression of AtHY5 was examined. Upon WWL + UVA exposure, the transcript levels of AtHY5 were up-regulated and reached the maximum at 4 h (the upregulation ratio of PpCRY1/cry1 was 13-fold, for cry1 mutant it was 3-fold, for WT it was 16-fold), then a decreased occurred with the increment of irradiation time ( Figure 5A). In comparison, AtHY5 in PpCRY1-overexpression seedling in cry1 background exhibited a similar transcript accumulation pattern as WT which was consistently higher than cry1 mutant. With complementation of the cry1 mutant, it can be concluded that PpCRY1 was a functional counterpart of AtCRY1 and could regulate AtHY5 expression in response to UVA irradiation.
Similarly, after five days growth under white light, WT, uvr8 mutant and PpUVR8.1/uvr8 transgenic lines were transferred to WWL + UVB condition for periods of 0-24 h. Before WWL + UVB irradiation (0 h), AtHY5 displayed relatively low transcription levels in all lines ( Figure 5B). After exposure to WWL + UVB, a UV-induced elevation in AtHY5 transcription occurred in WT, while no increase was observed in uvr8. However, the overexpression of PpUVR8.1 in uvr8 restored the ability of UVB to increase AtHY5 transcription. The transcript levels of AtHY5 showed a similar pattern in seedlings of PpUVR8.1/uvr8 transgenic lines and WT during the irradiation period, decreasing gradually after a rapid peaking within 4 h. This suggested that the PpUVR8.1 was a functional counterpart of AtUVR8 and could regulate the expression of AtHY5 in response to UVB irradiation.
As described above, AtHY5 participates in both UVA and UVB signaling pathways in the PpCRY1 and PpUVR8.1 dependent FIGURE 4 | Hypocotyl length (A), anthocyanin content (B) and the relative expression levels of anthocyanin biosynthesis related genes (C) in 5-day-old seedlings of wild type (WT), mutant (hy5, cop1) and transgenic plants (PpHY5/hy5, PpCOP1.1/cop1) grown under long-day condition (16 h 40 w/m 2 white light/8 h dark cycle). For Arabidopsis transgenic plants, three independent lines for each target gene were used. Data were presented as means (±SE) from three independent biological replicates with 30 or more Arabidopsis seedlings collected for each replicate. Values labeled with different letters indicated a significant difference at P < 0.05. manner respectively, leading to regulation of hypocotyl elongation and anthocyanin biosynthesis. The PpHY5/hy5 transgenic line was used for measuring hypocotyl length and anthocyanin content under WWL, WWL + UVA, WWL + UVB or WWL + UVA + UVB condition. Among hy5, WT and PpHY5/hy5 transgenic line under different light conditions, hy5 consistently showed the longest hypocotyl length and the lowest anthocyanin content relative to other plants (Figure 6). When overexpressing PpHY5 in hy5, the phenotypes of inhibition of hypocotyl elongation and anthocyanin accumulation were restored to levels in WT. Upon UV light exposure, the hypocotyl length was suppressed and anthocyanin increased in PpHY5/hy5 transgenic line and WT as compared with those under WWL, but such phenotypes of photomorphogenesis were compromised in hy5 (Figure 6). A more profound effect on photomorphogenesis was observed when irradiated with both UVA and UVB (Figure 6). The observation was consistent with the result in peach fruit under UV light treatments, indicating that PpHY5 played a similar role to AtHY5, which directly participated in UVA-and UVB-induced photomorphogenic responses and acted as a central positive regulator downstream of PpCRY1 and PpUVR8.1 photoreceptors.

PpMYB10.1, and Its Own Expression
Previous studies have demonstrated that Arabidopsis HY5 binds to the G-box (CACGTG) and ACE (ACGT containing element) motifs in the promoters of target genes in response to light (Lee et al., 2007). In Arabidopsis PpHY5/hy5 transgenic lines, it was found that PpHY5 promoted the expression of anthocyanin biosynthetic structural genes ( Figure 4C). In order to elucidate the function of PpHY5 in the regulation of anthocyanin . For Arabidopsis transgenic plants, three independent lines for each target gene were used. 5-day-old seedlings grown under constant WWL were subjected to WWL + UVA or WWL + UVB irradiation for the indicated time span. Data were presented as means (±SE) from three independent biological replicates with 30 or more Arabidopsis seedlings collected for each replicate. accumulation in peach, the promoter regions (1,500 bp length) of anthocyanin biosynthetic genes and PpMYB10s were isolated.
Analysis of promoter sequences revealed the presence of putative cis-regulatory elements related to light signaling. The HY5-binding motifs (G-box and/or ACE motifs) were distributed in the promoters of 11 genes (PpCHS1, PpCHS2, PpCHI, PpF3H, PpF3'H, PpDFR1, PpANS, PpUFGT, and PpMYB10.1/2/3), with the total number ranging from one to seven for each promoter ( Figure 7A). For example, five G-box motifs were identified in the PpCHS1 promoter, four G-box motifs, and one ACE motif were identified in the PpMYB10.1 promoter, while only one G-box motif and one ACE motif were identified in the PpANS promoter ( Figure 7A). To validate the effect of PpHY5 on the transcription of anthocyanin biosynthetic genes, the promoter sequences of 11 genes were isolated and the dual-luciferase assay was conducted. When co-infiltrated with PpHY5, the highest activity was observed in the promoter of PpCHS1 (5.3-fold) followed by PpMYB10.1 (4.9-fold), PpCHS2 (4.1-fold), and PpDFR1 (3.3-fold) ( Figure 7B). However, no obvious enhancements in luciferase enzyme activities were detected for other genes. Similarly, infiltration of PpHYH was also able to activate the promoters of both PpCHS1 (2.9-fold) and PpDFR1 (2.6-fold), while no obvious change in activation was observed on the promoters of either PpCHS2 or PpMYB10.1 or other genes ( Figure 7B). Compared with PpHY5, PpHYH showed a similar but weaker ability to activate the promoters of both PpCHS1 and PpDFR1. Taken together, these findings indicated that PpHY5 functioned as a positive transcriptional regulator for anthocyanin accumulation via activation of anthocyanin biosynthetic genes, possibly via binding directly to their promoters at G−box and ACE motifs.
We also analyzed the effects of PpHY5 and PpHYH on the promoter activity of PpHY5 itself. PpHY5 and PpHYH significantly stimulated PpHY5 promoter activity with the ratio of LUC to REN of 6.6 and 4.3 respectively (Figure 7C). To investigate the specificity of the interaction between PpHY5 and the PpHY5 promoter, we performed a combination of cisregulatory element mutations and the dual-luciferase assays to compare the activation levels. Four predicted E-box motifs were found in the promoter of PpHY5 and designated as E-box-1, E-box-2, E-box-3 and E-box-4 respectively ( Figure 7D). As shown in Figure 7E, when E-box-2 (CAAATG) within the PpHY5 promoter was mutated to ACAAGT, the trans-activation activity of PpHY5 on PpHY5-m2 promoter was attenuated by 74%. In contrast, PpHY5 still showed similar activation towards other mutated PpHY5 promoters (PpHY5-m1, PpHY5-m3 and PpHY5-m4) with the induction of 5.6-, 7-, and 6.4-fold, with FIGURE 6 | Effect of different light treatments on hypocotyl length (A) and anthocyanin content (B) of 7-day-old seedlings of wild type (WT), hy5 mutant and PpHY5/hy5 transgenic plant. Seedlings were grown under continuous weak white light (WWL, 40 µw/cm 2 ) supplemented with/without UVA (40 µw/cm 2 ) or UVB (10 µw/cm 2 ) or both together for different UV light treatments. For Arabidopsis transgenic plant, three independent lines were used. Data were presented as means (±SE) from three independent biological replicates with 30 or more Arabidopsis seedlings collected for each replicate. Values labeled with different letters indicated a significant difference at P < 0.05. no significant difference relative to the native PpHY5 promoter ( Figure 7E). These results suggested that PpHY5 might recognize E-box-2 to specifically activate its own transcription.

UVA and UVB Increased Anthocyanin Accumulation in Peach by Up-Regulating the Expression of Anthocyanin-Related Genes
Peach is an economically important fruit crop cultivated worldwide. Fruit coloration is one of the critical traits of exterior quality and commercial value for the consideration in consumer choice. Anthocyanin is the predominant component responsible for red pigmentation in peach fruit (Cheng et al., 2014). Light is an essential environmental factor influencing the production of anthocyanin and fruit coloration in peach, indicating the light signaling pathway is involved in the process (Zoratti et al., 2014). In this study, we conducted the comprehensive analysis of the effects of different UV lights on anthocyanin accumulation in peach. The results showed that peach fruit accumulated anthocyanin under UVA or UVB treatment and the induction was strengthened by the irradiation of UVA and UVB together (Figure 1). The induction was due to enhanced expression of genes encoding enzymes of the anthocyanin biosynthetic pathway.
The effects of UV irradiation on anthocyanin accumulation were not apparent until after continuous UV light treatment for 72 and 144 h. However, the transcript levels of multiple genes were induced in a short time (Figure 2). The results were similar with the significant elevation in the transcripts of AtCHS, AtF3H, and AtDFR in Arabidopsis under blue light treatment for 1 h (Cominelli et al., 2008). Maximum transcriptional response was observed following irradiation with of UVA + UVB, which correlated with anthocyanin accumulation. The molecular evidence of the synergistic effect of UVA + UVB on the regulation of anthocyanin biosynthesis needs further study.
The Function of PpHY5 Is Conserved in Different Light Signaling Pathways for Anthocyanin Accumulation HY5, a member of the bZIP family transcription factor, is an essential regulator in multifaceted developmental processes, The ratio of LUC/REN of the empty vector (SK) plus promoter was used as the calibrator (set as 1). LUC: firefly luciferase; REN: renilla luciferase; green circles: G-box; blue triangles: ACE motif; blue circles: E-box; red circles: mutated E-box. Data were presented as means (±SE) from six independent biological replicates and Student's t-test was used for statistical analyses compared with corresponding control. Asterisks ( * * ) indicated a highly significant difference at P < 0.01. such as photomorphogenesis, pigment accumulation, chloroplast development, nutrient assimilation, and carbon/nitrogen balance (Gangappa and Botto, 2016). HY5 has also been functionally characterized to be involved in the regulation of anthocyanin accumulation in response to light in a variety of plants (Shin et al., 2013;Jiang et al., 2016;An et al., 2017). After exposure to blue light, the transcript level of SlHY5 in tomato was induced sharply within 3 h, and the SlHY5 protein accumulated steadily and peaked at 48 h . In peach, PpHY5 had a strong response to both UVA and UVB at the transcription level, and the expression rapidly increased within 6 h. Expression of PpHYH, the closest homolog of PpHY5, was also induced by UVA and UVB treatments. Meanwhile, a decrease in the transcription level of PpCOP1.1 was observed.
In Arabidopsis under white light, the hy5 mutant exhibited skotomorphogenic phenotype with less anthocyanin content and longer hypocotyl length, conversely the cop1 mutant presented photomorphogenic phenotype with higher anthocyanin content and short hypocotyl length. Overexpression of PpHY5 in hy5 and PpCOP1.1 in cop1 restored wild type phenotypes under white light ( Figure 4A). Moreover, the RT-qPCR analysis showed that anthocyanin biosynthetic structural genes, including AtPAL, AtCHS, AtCHI, AtF3H, AtDFR, AtANS, and AtUFGT, were significantly up-regulated in PpHY5/hy5 and down-regulated in PpCOP1.1/cop1 transgenic lines than those in the corresponding mutant ( Figure 4C). The phenotypes in Arabidopsis transgenic lines and mutants implied that PpHY5 and PpCOP1.1 acted as counterparts. Under WWL, PpHY5 also directly participated in UVA-and UVB-induced anthocyanin accumulation which depended on PpCRY1 and PpUVR8.1 photoreceptors, indicating that PpHY5 exhibited a positive regulatory role in anthocyanin biosynthesis in response to various wavelengths of light environment.
We further investigated the role of PpHY5 in the regulation of anthocyanin accumulation using the dual-luciferase assay. PpHY5 positively activated the transcription of PpCHS1, PpDFR1, PpCHS2, and PpMYB10.1 (Figure 7B). This is similar to results seen in tomato, apple, and pear Liu et al., 2018;Tao et al., 2018). We also found that PpHYH had a similar effect to PpHY5 in regulating anthocyanin accumulation ( Figure 7B). In addition, PpHY5 could bind to the E-box to regulate its own transcription ( Figure 7E). As hypothesized, UVA and UVB light signals for anthocyanin biosynthesis were via PpHY5 in peach (Figure 8). These findings suggested that PpHY5 had a conserved function across different species and various wavelengths of light in regulating anthocyanin accumulation.

PpCRY1 and PpUVR8.1 Contributed to UVA and UVB Signaling Pathways in Peach
Many studies have revealed that the photoreceptors are indispensable for anthocyanin biosynthesis. VviUVR1 and MpUVR8, the UVB receptors in grape and liverwort, played a FIGURE 8 | A possible model for UVA-and UVB-induced anthocyanin biosynthesis in peach fruit. The UV light receptors PpCRY1 and PpUVR8.1 respectively perceive UVA and UVB light signals and influences the expression of PpHY5, an essential regulator in UVA and UVB signaling pathway. PpHY5 positively induces its own transcription by interacting with an E-box in its own promoter and activates the expression of PpMYB10.1 to regulate anthocyanin accumulation. The transcription of anthocyanin biosynthetic structural genes is directly regulated by PpMYB10.1 and some of them were also regulated by PpHY5. The blue arrows indicate the pathways verified in the present work. The black arrows indicate the pathways that have been previously reported in peach.
predominant role in the UVB-induced flavonoid accumulation (Loyola et al., 2016;Clayton et al., 2018). The phenotypes of alleviated hypocotyl elongation inhibition and decreased anthocyanin accumulation were observed in the antisense Brassica napus CRY1 (BnCRY1) transgenic plant (Chatterjee et al., 2006). In apple, cryptochrome genes MdCRY1 and MdCRY2 have been identified to regulate anthocyanin accumulation and MdCRY2 also participated in the photoperiodic control of flowering (Li et al., 2013a,b). Overexpression of SlCRY1a led to higher anthocyanin accumulation in tomato  and SlUVR8 mediated the expression of SlCHS in response to the UVB environment (Li et al., 2018). We cloned the PpCRY1 and PpUVR8.1 from peach and characterized their functions as UVA and UVB receptors by complementation of Arabidopsis mutants. In our study, overexpressing PpUVR8.1 or PpCRY1 in the corresponding transgenic line promoted the expression of AtHY5 in response to UVA or UVB irradiation, respectively. The results provided evidences that PpCRY1 and PpUVR8.1 acted as the photoreceptors of UVA and UVB and were associated with eliciting the transcript accumulation of HY5.
In conclusion, our study showed that UVA and UVB accelerated anthocyanin biosynthesis in the peel of "Hujingmilu" peach. The functions of crucial genes and photoreceptors in UV light signaling transduction pathways from peach were clarified. The data suggested that PpHY5 was both involved in UVA-and UVB-induced anthocyanin accumulation via a relatively conserved signaling pathway in PpCRY1-dependent and PpUVR8.1-dependent manner, respectively (Figure 8). PpHY5 regulated anthocyanin accumulation by activating the expression of anthocyanin biosynthetic-related genes. However, the results were mainly based on transcriptional control, which still needs to be verified at the post-transcriptional level in future experiments. Additionally, it would be worthy to investigate whether a direct interaction or an indirect interaction containing other unknown factors exists between PpCRY1 or PpUVR8.1 and PpHY5.

DATA AVAILABILITY STATEMENT
The raw data were deposited in NCBI sequence read archive (SRA) under accession number PRJNA665861.

AUTHOR CONTRIBUTIONS
YZ carried out the experiment, analyzed the data, and drafted the manuscript. MC helped to provide the experiment materials. CZ and RJ helped to provide technical assistance. TM, HW, AA, KL-W, and CX were involved in the revision of the manuscript. CX initiated the project, designed the research framework, coordinated the study, and participated in writing the manuscript. All authors read and approved the final manuscript.