An Overview of the Characteristics and Potential of Calotropis procera From Botanical, Ecological, and Economic Perspectives

Calotropis procera (Aiton) Dryand. (commonly known as the apple of sodom, calotrope, and giant milkweed) is an evergreen, perennial shrub of the family Apocynaceae, mainly found in arid and semi-arid regions. It is a multipurpose plant, which can be utilized for medicine, fodder, and fuel purposes, timber and fiber production, phytoremediation, and synthesis of nanoparticles. It has been widely used in traditional medicinal systems across North Africa, Middle East Asia, and South-East Asia. At present, it is being extensively explored for its potential pharmacological applications. Several reports also suggest its prospects in the food, textile, and paper industries. Besides, C. procera has also been acknowledged as an ornamental species. High pharmacological potential and socio-economic value have led to the pantropical introduction of the plant. Morpho-physiological adaptations and the ability to tolerate various abiotic stresses enabled its naturalization beyond the introduced areas. Now, it is recognized as an obnoxious environmental weed in several parts of the world. Its unnatural expansion has been witnessed in the regions of South America, the Caribbean Islands, Australia, the Hawaiian Islands, Mexico, Seychelles, and several Pacific Islands. In Australia, nearly 3.7 million hectares of drier areas, including rangelands and Savannahs, have been invaded by the plant. In this review, multiple aspects of C. procera have been discussed including its general characteristics, current and potential uses, and invasive tendencies. The objectives of this review are a) to compile the information available in the literature on C. procera, to make it accessible for future research, b) to enlist together its potential applications being investigated in different fields, and c) to acknowledge C. procera as an emerging invasive species of arid and semi-arid regions.


INTRODUCTION
Calotropis procera (Aiton) Dryand. is a soft-wooded, perennial shrub of the family Apocynaceae and subfamily Asclepiadaceae (the milkweed family). It is an evergreen xerophytic plant, generally found in arid and semi-arid habitats (Al-Rowaily et al., 2020). The word "Calotropis" is derived from Greek, meaning "beautiful, " which refers to its flowers; whereas "procera" is a Latin word referring to the cuticular wax present on its leaves and stem . It is known by various common names such as apple of sodom, calotrope, giant milkweed, Indian milkweed, wild cotton, rubber tree, ushar, etc., in different parts of the world. Its subspecies, C. procera subsp. procera and C. procera subsp. hamiltonii, vary from each other in fruit morphology (Dhileepan, 2014). It also shares a close homology with its con-generic plant C. gigantea (CABI, 2021).
Calotropis procera is a multipurpose plant, which provides a wide range of provisioning ecosystem services. It has been widely used in traditional medicinal systems in North Africa, Middle East Asia, South Asia, and South-East Asia (Al Sulaibi et al., 2020). It has also been utilized for fiber, fuel, fodder, and timber purposes since antiquity (Batool et al., 2020). Owing to its socio-economic importance, it has been introduced in several parts of the world outside its native range (Asia and Africa). Morpho-physiological adaptations and the ability to tolerate a wide range of environmental conditions enabled its naturalization in the introduced habitats. Consequently, the plant has also been reported as an invasive weed of wastelands, overgrazed pastures, and poorly managed agricultural fields in several regions (CABI, 2021).
There is a plethora of literature available that demonstrates the pharmacological applications and economic importance of C. procera. However, very few studies have focused on general ecological and biological characteristics of the plant and its survival strategies under arid and semi-arid environments. Even fewer studies have addressed it as an invasive species and provided insights into its invasive abilities, potential distribution, and management options. In this review, multiple aspects of C. procera have been discussed to bring together the information available on the plant in the literature, identify its potential applications, acknowledge it as an emerging invasive species, and emphasize the knowledge gaps in ongoing research.

ECOLOGY AND BIOLOGY Geographical Distribution, Macromorphology, and Reproductive Biology
Calotropis procera is native to Africa, Arabian Peninsula, Western Asia, the Indian Subcontinent, and Indo-China (GRIN, 2021). However, the introduction of the plant outside its native boundaries has led to its naturalization in parts of Africa, Australia, and America (GRIN, 2021). The broad native and exotic geographical range of C. procera is presented in Figure 1. Calotropis procera is an evergreen shrub that may grow up to 6 m (usually 2.5-4 m) in height and has a deep taproot system (CABI, 2021; Figure 2). Young stems are grayish-green in color, smooth, and pubescent, whereas the mature stems have a deeply fissured bark . The leaves are large, pale green, succulent, arranged in opposite phyllotaxy, and covered with cuticular wax (Batool et al., 2020; Figure 2). The plant contains a milky sap, which oozes out of any wound or injury in the aboveground parts (CABI, 2021; Figure 2).
Reproductive maturity in the plant is attained approximately 190 days after germination (Bebawi et al., 2015). Flowering takes place throughout the year, and pollination is carried out by insects, mostly bees and butterflies (Al Sulaibi et al., 2020;Batool et al., 2020). The inflorescence is dense and multiflowered umbellate cyme (3-15 flowers in a cluster; Figure 2), and the flowers are five-petaled, bisexual, sweetsmelling, and white in appearance with a characteristic purple tip (Al Sulaibi et al., 2020;Figure 2). Fruiting is limited to the warm months of the year when pollinators are the most abundant (Menge et al., 2017a). The fruits are ellipsoid or ovoid, containing 350-500 seeds with tufts of white, silky hair or pappus (Al Sulaibi et al., 2020; Figure 3). Seeds are generally disseminated by wind and water and occasionally, by birds and animals (Al Sulaibi et al., 2020). Seed longevity depends on several factors such as rainfall, soil moisture, seed burial depth, and soil type (Bebawi et al., 2015). Maximum seed germination (68-100%) occurs at 30 • C and the maximum emergence (88%) is observed from a depth of 3 cm (Menge et al., 2016a). The plant also propagates through root suckers and regenerates through broken/cut stems and roots .

Stress Physiology
Calotropis procera has an exceptional ability to adapt and maintain productivity in severe arid conditions (Ramadan et al., 2014). It is a C 3 plant that can survive drought, salinity, extreme temperatures, high vapor pressure deficit, and high photosynthetic active radiations (Frosi et al., 2013;Rivas et al., 2020). It can easily thrive in prolonged dry seasons with rainfall >150 mm per year (Dhileepan, 2014). The plant grows abundantly in xerophytic conditions on a variety of soils, without irrigation or application of fertilizers . The plant has a great potential to endure stress caused by roadside pollutants and contaminated soils (Khalid et al., 2018;Ullah and Muhammad, 2020).
Plants surviving in the hostile environment of arid/semi-arid regions have advanced morpho-physiological adaptations and special defense mechanisms. So is the case of C. procera, in which multiple processes contribute to the resistance, resilience, and recovery of individuals growing under abiotic stress conditions (Rivas et al., 2017). The stems and leaves of C. procera are characterized by thick cuticle, lactiferous canals, and low specific leaf area (Tezara et al., 2011;Hassan et al., 2015). Leaves are found to be narrower and thicker under optimum moisture conditions, whereas they are broader and thinner under dry conditions (Pompelli et al., 2019). These factors help in the conservation of acquired resources and creating a water permeability barrier, thereby reducing the transpiration rate (Pompelli et al., 2019).
The plant also shows physiological and biochemical adaptations in terms of gas exchange and metabolic adjustments (Frosi et al., 2013). An efficient antioxidative system, leaf sugar dynamics, and photoprotective mechanisms guard the photosynthetic machinery of the plant under an extreme xerophytic environment (Rivas et al., 2017(Rivas et al., , 2020. Furthermore, the plant maintains a high photosynthetic rate despite reduced stomatal conductance, thus increasing water use efficiency, which is a fundamental characteristic for survival in arid and semi-arid ecosystems (Tezara et al., 2011;Frosi et al., 2013) as well as being able to quickly adjust the aquaporins of the root system when under salt stress (Coêlho et al., 2021). A metabolomic study revealed that C. procera rapidly adjusts the levels of soluble sugars, amino acids, triacylglycerols, and membrane lipids in response to water availability and water loss (Ramadan et al., 2014). Myo-inositol signaling is found to be induced in response to drought and salt stress in C. procera (Mutwakil et al., 2017).

Metabolic Profile
Several researchers have reported the presence of metabolites such as flavonoids, tannins, terpenoids, saponins, alkaloids, steroids, and cardiac glycosides in various parts of the plant (Mossa et al., 1991;Moustafa et al., 2010;Al-Rowaily et al., 2020). A list of secondary metabolites reported from the plant has been provided in Table 1.
A total of 80% of the laticifer fluid of C. procera corresponds to rubber and the rest 20% is rich in basic proteins (anti-oxidant enzymes, cysteine proteases, tryptophan, etc.) with molecular masses in the range of 5-95 kilodaltons (Freitas et al., 2007;Das et al., 2011). A recent study deduced amino acid sequences of five previously identified cysteine peptidases from the latex of C. procera (procerain, procerain B, CpCP1, CpCP2, and CpCP3) . These possess similar biochemical characteristics and high sequence homology with several other papain-like cysteine peptidases . The presence of nearly 15 chitinase isoforms has also been reported in the latex of C. procera (Freitas et al., 2016).

Cytotoxicity and Phytotoxicity
Calotropis procera causes acute toxicity in various plant and animal cells, including human beings. Different plant parts, particularly the latex, are therefore tested against various cancer cell lines (Ibrahim et al., 2015;Viana et al., 2017;Al-Qahtani et al., 2020). Similarly, antibacterial and antihelminitic potential of the plant is being utilized in pharmacology (details provided in section "Pharmacological Applications"). However, the toxicity-bioactivity relationship of C. procera is still not well investigated. A few studies suggested that the plant induces acute cardiotoxicity and hepatotoxicity (de Lima et al., 2011). On the other hand, a safety evaluation study by Mossa et al. (1991) revealed that the use of C. procera extract in single high doses (up to 3 g kg −1 ) is not toxic for guinea pigs until the treatment of >90 days is provided. In another study, latex proteins of the plant when administrated orally, had no adverse immunological reactions in mice even at 5,000 mg kg −1 ; but their intraperitoneal administration caused death after 1 h in response to a dose of 150 mg kg −1 (Bezerra et al., 2017). These toxic aspects are not extensively researched and more studies are required to validate the medicinal prospects of C. procera. Apart from that, extracts of the plant also possess significant pesticidal and fungicidal properties. It has been observed that lifehistory traits of Sitophilus oryzae L. (Coleoptera: Curculionidae) and Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae) were modulated by leaf extracts, latex proteins, and flavonoids isolated from C. procera (Nenaah, 2013a). Whole-plant extracts of the plant caused mortality of larva, reduced the number of eggs, and inhibited the oviposition of Rhipicephalus microplus Canestrini (Ixodida: Ixodidae) (Khan et al., 2019). Cysteine peptidases and osmotin purified from the latex of C. procera promoted membrane permeability, leakage of cellular content, and induction of reactive oxygen species in Fusarium spp. Freitas et al., 2020). Such studies implicate that plant has a potential to be utilized as bioinsecticide and biofungicide in agricultural and industrial practices.

Pharmacological Applications
The search for environment-friendly prototypes to replace chemically synthesized drugs is rapidly increasing. Thus, a lot of research has been focused on the plant species mentioned in traditional medicinal systems. The pharmacological activities of C. procera have been popular in the past to cure several diseases in human beings such as cold, fever, leprosy, asthma, rheumatism, eczema, indigestion, diarrhea, elephantiasis, skin diseases, and dysentery (Al-Rowaily et al., 2020). The decoction of aboveground parts is being used to treat fever, joint pain,    Frontiers in Plant Science | www.frontiersin.org muscular spasm, and constipation in Saudi Arabia (Mossa et al., 1991). The plant is also used to treat neuropsychiatric disorders in Burkina Faso (Kinda et al., 2020). The medicinal attributes of C. procera can be credited to secondary metabolites and cardiotonic substances present in the plant (Hagaggi and Mohamed, 2020;Mehmood et al., 2020). The extracts of aboveground plant parts of C. procera exhibited strong antipyretic, analgesic, antidepressant, and neuromuscular blocking activity (Mossa et al., 1991;Garabadu et al., 2019). Extracts from bark and leaves showed notable antibacterial potential against Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli (Mehmood et al., 2020). A broad antibacterial spectrum has been shown by extracts of both aerial parts of C. procera and its endophytic bacteria, Bacillus siamensis (Hagaggi and Mohamed, 2020). Leaf extracts of C. procera also reduce blood glucose to a significant level, thereby indicating its antihyperglycemic potential (Nadeem et al., 2019).
Latex of C. procera contains cardiac glycosides, which inhibit the proliferation of MCF-7 cells through cytotoxicity, apoptosis, and autophagy (Al-Qahtani et al., 2020). Chitinase isoforms present in the latex are also cytotoxic to tumor cell lines and are capable of reducing inflammation by iNOs-derived NO mechanism . Crude latex also possessed antioxidant and antiapoptotic activities against the toxicity of 4-Nonylphenol (Sayed et al., 2016). It has shown anthelmintic effects against Haemonchus contortus by damaging its cuticle and causing ultrastructural changes (Cavalcante et al., 2020). Latex of C. procera is also a promising phytotherapeutic option for treating inflammatory conditions of the colon (Kumar et al., 2019). The protein fraction of the latex has the potential to relieve inflammation and pain associated with arthritis (Kumar et al., 2011). Oral mucositis, an intense inflammatory reaction that can lead to tissue damage and ulceration, was found to be curable using PII-IAA, a homogenous cocktail of laticifer proteins of C. procera . Similarly, intestinal mucositis is observed to be abolished by latex proteins of C. procera (de Alencar et al., 2017).
In addition to that, anti-inflammatory and gastromucosal protective effect of the stem bark of C. procera has also been observed (Tour and Talele, 2011). Root bark also consists of oxypregnane oligoglycosides, which has cytotoxic potential against U373 glioblastoma and PC-3 prostate cancer cell lines (Ibrahim et al., 2015). An earlier retrieval of sensorimotor activities, reduced ROS, increased total antioxidant activity (particularly, the enhanced activities of arylesterase and paraoxonase), suggested a positive impact of roots of C. procera on functional recovery upon a nerve injury (Zafar et al., 2020).

Phytoremediation
Calotropis procera is a phytoaccumulator of several heavy metals such as manganese, lead, chromium, iron, copper, nickel, cobalt, strontium, and cadmium (D'Souza et al., 2010;Almehdi et al., 2019;Ullah and Muhammad, 2020). As determined from biophysical measurements, roots and leaves of C. procera are also tolerant against aluminum toxicity (Hussain et al., 2018). C. procera can also be used as a phytomonitoring tool to assess metals in the environment (Gajbhiye et al., 2019). A high accumulation of chromium has been observed in the roots (up to 188.2 mg kg −1 ) and shoots (up to 68.2 mg kg −1 ) of C. procera, which is detoxified by regulation of cellular homeostasis via redox signaling (Usman et al., 2020). Fruits and leaf powder of C. procera were also found to adsorb, respectively, Acid red 73 and Congo Red dye, the colorant dyes used in dyeing processes, which are harmful to aquatic life due to their release in the water bodies (Kaur and Kaur, 2017;EL-Adawy and Alomari, 2020). It has also been observed that old leaves of the plant have a greater ability to accumulate heavy metals compared to any other plant parts (Almehdi et al., 2019). This suggests that C. procera uses the metabolically less active leaves as sinks for heavy metals (Almehdi et al., 2019).

Source of Fiber
Calotropis procera is an emerging source of natural fiber. Efforts have been put to screen efficient genotypes from its wild populations, which can be improved through conventional breeding programs to develop suitable varieties for cultivation (Majeed et al., 2020). Its fiber is natural, renewable with low density, high strength, crude oil sorption capacity (about 75 times its weight), and hydrophobic-oleophilic characteristics (Hilário et al., 2019;dos Anjos et al., 2020;Raghu and Goud, 2020). It is composed of 64.0 weight % cellulose, 19.5 weight % hemicelluloses, and 9.7 weight % of lignin (Song et al., 2019). The fibers exhibit thermal stability and can endure a temperature up to 200 • C (Yoganandam et al., 2019). Alkali treatment may enhance the tensile strength, modulus, and length of the fiber (Raghu and Goud, 2020). The chemical polymerization of polyaniline enhances fiber conductivity (dos . For increasing the absorption efficiency of organic oils and solvents, the fiber can be treated with 0.1 M sodium hydroxide or 1% sodium chlorite (dos Anjos et al., 2020). Also, fiber length can be improved by a cell expansion mechanism derived from plasma membrane intrinsic proteins (Aslam et al., 2013).
Owing to its antimicrobial tendency, the bast fiber from C. procera can substitute cotton (Gossypium sp.) wool for surgical or stuffing purposes (Basu, 2020). Stuffing material for mattresses and pillows can also be prepared from the fiber (Oun and Rhim, 2016). These natural fibers are also promising candidates for the fabrication of composites (Yoganandam et al., 2020) and the production of cellulose nanocrystals (Song et al., 2019). Reports suggest that fiber of C. procera can also be used as a biosorbent for the removal of contaminants due to oil spill (Hilário et al., 2019;dos Anjos et al., 2020).

Synthesis of Nanoparticles
Green nanotechnology has become an emerging field for the costeffective and eco-friendly production of metallic nanoparticles (NPs) for multiple industrial applications, and C. procera has successfully facilitated their fabrication. Cysteine proteases present in the latex were used to produce copper and gold NPs, which showed excellent biocompatibility with HeLa, A549, and BHK21 cell lines (Das et al., 2011;Harne et al., 2012). Silver NPs prepared using latex of C. procera showed strong antibacterial and antifungal activities (Mohamed et al., 2014). Cerium oxide NPs produced using C. procera flower extract have proved to be effective against gram-negative bacteria (Muthuvel et al., 2020). The therapeutic potential of silver NPs containing root extracts of C. procera was found to be significant against 10 strains of medically important bacteria and human epidermal primary keratinocytes cell line due to the metal-phytochemical moiety (Sagadevan et al., 2020). Similarly, iron NPs prepared in the leaf extracts are found to be efficient, cost-effective, and eco-friendly with strong antifungal activity (Ali et al., 2020a).

Miscellaneous
Calotropis procera is used as an alternative for fodder during dry periods when other plant species are scarce (Frosi et al., 2013). Its use for fuel, timber, and building purposes dates back to the nineteenth century (Al Sulaibi et al., 2020;Batool et al., 2020). The plant has also been acknowledged for its ornamental value (de Oliveira et al., 2009). The plant yields valuable hydrocarbons and holds the potential to produce bioenergy and biofuel, which could be used as diesel substitutes in the future (Kumar, 2018). Studies also recommend the use of its enzyme extract to tenderize muscle foods such as pork, beef, and chicken (Rawdkuen et al., 2013), dehair crude leather (Lopéz et al., 2017), and coagulate milk for the production of fresh cheese (Abebe and Emire, 2020). C. procera leaves are also a potential source of natural colorants for textile fabrics (Hussaan et al., 2017). Cuticular wax derived from the plant is an eco-friendly hydrophobic material, which can have several industrial applications . Apart from that, C. procera is one of the alternative raw materials for making excellent varieties of handmade paper (Aswal et al., 2020).

CALOTROPIS PROCERA AS AN INVASIVE SPECIES
Calotropis procera is a native of Asia and Africa but widely naturalized throughout the arid and semi-arid parts of the world (as described in section "Geographical Distribution"). Owing to its spread in new and larger areas, and adverse effects on the native ecosystems, C. procera has been declared as an invasive species in several regions of the world. It is a serious environmental weed of South America, the Caribbean Islands, Australia, the Hawaiian Islands, Mexico, Seychelles, and several Pacific Islands (Dhileepan, 2014).
In South America, the plant was introduced for ornamental and forage purposes; however, it has spread beyond the introduced areas by colonizing habitats with different environmental characteristics (Rivas et al., 2020). It is said to be an aggressive invader of the Caatinga ecoregion (Al Sulaibi et al., 2020) and others regions of northeastern Brazil where it has been introduced at the beginning of the nineteenth century (Frosi et al., 2013). The probability of its spread in the Canga ecoregion of Espinhaço mountain ranges of Brazil has also been suggested (de Oliveira et al., 2009). Due to its fast growth and drought tolerant abilities, it has spread extensively in the Caribbean Islands (Pompelli et al., 2019). Recently, it has been reported to spread along the coastal dunes of the Caribbean region of Colombia (Gracia et al., 2019).
In Australia, the plant may have introduced intentionally as an ornamental or accidentally with the packaging of camel saddles from India in the early 1900s (Dhileepan, 2014). It was reported from Katherine, Northern Territory, for the first time in the 1950s and thereafter, it has spread up to 3.7 million ha in drier parts of Northern Territory, Western Australia, and Queensland (Dhileepan, 2014). It has invaded the rangelands and Savannahs of Australia, threatening their biodiversity and productivity (Campbell et al., 2013(Campbell et al., , 2020. In the Gulf of Carpentaria region, its infestations have increased tremendously within the past few years and it has now approached the Burdekin catchment (Campbell et al., 2013). C. procera has also colonized in the rehabilitated Mary Kathleen uranium mine site in Queensland, Australia (Lottermoser, 2011). Ecological modeling based on climate change projections suggests that the uninvaded regions of northern and north-eastern territories of Western Australia and north-western Queensland are at potential risk of invasion by C. procera (Menge et al., 2016b).
Calotropis procera adopts an adult-persistence-populationsurvival strategy, characterized by lesser recruitment of fresh seedlings and relative stability of adult populations . It can grow in a wide range of open habitats, such as along roadsides, watercourses, riverbeds, coastal dunes, deserts, semi-deserts, scrublands, overgrazed pastures, and disturbed areas (Dhileepan, 2014;Hassan et al., 2015). Being a metallophyte, C. procera invades polluted areas, contaminated sites, rehabilitated mines, ironstone rupestrian fields, etc., as pioneer vegetation (de Oliveira et al., 2009;Lottermoser, 2011). It also has a widespread persistence near unmanaged crop fields and thus, it may impose adverse effects on the crops through allelopathy .
A phenological study of C. procera stated that ornamental and economic value of the plant leads to its distribution across the globe and functional traits such as large leaves, winddispersed seeds, hermaphrodite flowers, and ability to attract pollinators have facilitated its invasion process (Sobrinho et al., 2013). A difference in the reproductive phenology between the individuals of invaded range and native range has also been observed, with individuals present in the invaded range having a longer reproductive window (Sobrinho et al., 2013). Plasticity in phenological and functional attributes enables it to dominate the urban ecosystems of South Cairo, Egypt Pompelli et al., 2019). Disturbance levels in the soil also affect seed establishment in C. procera, and therefore, its uncontrolled spread is witnessed in areas subjected to natural and anthropogenic interference (Menge et al., 2017b). Also, C. procera is capable of defending itself against herbivores by producing latex with toxic steroidal cardenolides and releasing irritating volatiles (Fernandes et al., 2020).
Currently, management options practiced for C. procera include mechanical removal, chemical control, and management of invaded or susceptible areas. The plant can be removed mechanically along with its roots to prevent reproduction via suckers . The use of mechanical equipment that severs the root system can achieve a mortality rate of up to 72% in C. procera, but the disturbance often promotes new seedling recruitments (Campbell et al., 2020). Foliar herbicides such as imazapyr, metsulfuron-methyl, 2,4-D butyl ester, fluroxypyr, triclopyr, and triclopyr plus picloram reported up to 80% efficacy in controlling the plants when applied to stump <5 cm in height (Vitelli et al., 2008). C. procera cannot stand competition with tall weeds, bushes, and grasses, and therefore, cannot invade intact grasslands (Menge et al., 2017b). Management is suggested in colder months when pollinator pressure is low and plants are not reproducing (Menge et al., 2017a). Because the plant needs nearly 1 year to produce fruits after emergence, conservation managers can manage its patch in a given area by constantly targeting new seedlings for 2 years to exhaust the seed bank (Bebawi et al., 2015).
In its native range, C. procera has several natural enemies that may act as potential biocontrol agents for the plant. A total of 65 insect species and five mite species have been reported to attack C. procera (Dhileepan, 2014). Among the herbivorous insects, larvae of Danaus spp. were observed to bypass host defenses, and feed on healthy, rapidly growing C. procera in the Brazilian Caatinga (Fernandes et al., 2020). The fruit fly, Dacus persicus Hendel (Diptera: Tephriti7dae) is also a prospective biological control agent for the plant in Australia owing to its field host specificity, high reproductive capacity, and damage potential (up to 100% damage to the immature seeds and 62% reduction in the biomass of infested fruits) (Ali et al., 2020b). Dhileepan (2014) suggested three pre-dispersal seed predators, Paramecops farinosus Schoenherr (Coleoptera: Curculionidae), D. persicus and Dacus longistylus Wiedemann (Diptera: Tephritidae) as prospective biocontrol agents of C. procera. Among the fungal pests, Passalora calotropidis is known to cause leaf spot disease in C. procera (Kiran et al., 2020). Since C. procera is an emerging invasive species of arid and semi-arid regions, suitable management strategies are needed to be devised and implemented as soon as possible so that spread and impact of the plant can be timely contained.

CONCLUSIONS AND FUTURE PROSPECTS
Calotropis procera is a plant with multifaceted biological characteristics that make it a medicinally and socio-economically important species on one hand and a potential invasive species on the other. The present discussion is meant to appraise its expanding global distribution, significant ecological and biological traits, applications in traditional and advanced fields, and infestation as an environmental weed. Also, it is an attempt to recognize the lesser-explored aspects and knowledge gaps in ongoing research.
Although pharmacological and industrial applications of the plant have received due attention, its general biological and ecological attributes (particularly those focusing on the adaptations or plasticity) have not been well-investigated. Also, the toxicity-bioactivity relationship of C. procera, which plays a key role in validating its medicinal aspects, has not been focused upon. Evaluating these basic facets may improve its commercial utilization and pave ways for novel applications. At the same time, covering these knowledge gaps can help understanding its invasive behavior and potential environmental or biodiversity threats that it can pose in the future.
In addition to that, the current and potential spread of C. procera is required to be mapped to carry out its timely management or containment, wherever required. The spread of C. procera can be effectively controlled in the invaded ranges via mechanical, chemical, or biological methods, followed by constant monitoring over the next few years to avoid new plantlets. Recognizing the plant as an important environmental weed can supplement its management programs at research, legislative, stakeholder, and local levels. Also, promoting its utilization at commercial and non-commercial scales can be an economically viable or better to say, economically beneficial way of its management.

AUTHOR CONTRIBUTIONS
DB and BC developed the initial concept and outline. AK and SK took lead in expanding the content. DB, SK, and BC contributed and edited the manuscript. All authors contributed to the article and approved the submitted version.

ACKNOWLEDGMENTS
AK is thankful to MOEF&CC, New Delhi, India, for providing financial support.