Assessment of the Relationship Between Executive Function and Cardiorespiratory Fitness in Healthy Older Adults

Associations between cardiorespiratory fitness and brain health in healthy older adults have been reported using a variety of cardiorespiratory fitness estimates (CRFe). Using commonly used methods to determine CRF, we assessed the relationship between CRFe and executive function performance. Healthy older adults (n = 60, mean age 68 years, 77% women), underwent three CRF tests: a Maximal Graded Exercise Test performed on a cycle ergometer, the Rockport Fitness Walking Test, and a Non-Exercise Prediction Equation. Executive function was assessed by a computerized cognitive assessment using an N-Back task (updating cost) and a Stroop task (interference cost, global and local switch cost). Multiple hierarchical regression analyses were conducted to assess the relationship between different CRFe and executive function performance. Regardless of age and education, cardiorespiratory fitness estimated from the Maximal Graded Exercise Test and the Rockport Fitness Walking Test was significantly associated with the global switch cost. All CRFe were associated with the interference cost. No association was observed between CRFe and local switching costs or the updating costs. In the present study, not all subcomponents of executive function were related to CRFe. Interestingly, the executive functions that were associated with CRFe are those that are known to be the most affected by aging.


INTRODUCTION
The long-term benefits of lifelong physical activity (Larson et al., 2006), particularly on cognitive aging (Gajewski and Falkenstein, 2015a;Berryman et al., 2018), have been extensively studied. However, more studies are needed to determine how physical activity interacts with brain health. One potential mechanism is cardiorespiratory fitness (CRF; Hayes et al., 2013). CRF, expressed aṡ VO 2 max, represents the body's maximal ability to transport and use oxygen, from the atmosphere to the working muscles (Ross et al., 2016). Integrating multiple physiological systems (pulmonary ventilation, cardiovascular function, and muscular work), CRF is positively associated with multiple health outcomes, such as cognitive functions (Hayes et al., 2016), as well as future cognitive states (Wendell et al., 2014). In older adults, participation in both acute and long-term moderate-to vigorous-intensity physical activity, such as aerobic exercise (known to increase CRF), has been shown to improved brain function (Erickson et al., 2019).
In the context of aging research, a Maximal Graded Exercise Test (GXT), the standard procedure for evaluatingVO 2 max, may not be feasible as it requires critical screening procedures and medical supervision to optimize safety (Huggett et al., 2005). To overcome these practical issues, the use of submaximal exercise tests [such as the Rockport Fitness Walking Test (ROCKPORT) or a Non-Exercise Prediction Equations (EQUATION)] may be recommended, although they are likely to be less precise than a GXT. For example, CRF estimate (CRFe) obtained with EQUATION, is often based on self-reported measures that may be subject to memory problems, social desirability, and participant subjectivity, which could lead to measurement errors (Wang et al., 2019).
In older adults (n = 86, M age 65.14, 61.6% women), a crosssectional study found significant associations between three CRFe (namely a GXT performed on a treadmill, the ROCKPORT, and EQUATION) and working memory, processing speed, and memory complaints (McAuley et al., 2011). In their report, the authors emphasized the importance of further research to better quantify potential associations between executive function (EF) and CRFe, particularly EQUATION. EF is required when the behavior or information processing to be performed is not automated and required some control (Klein, 1996). This control may be necessary because of the unpredictability or novelty of the situation (Banich, 2009). EF is also needed when two tasks cannot be processed in parallel in working memory (de Fockert et al., 2001) or consecutively without affecting performance (flexibility or switching). The second characteristic of EF is that they are initiated proactively, voluntarily (even consciously) (Lezak, 1995), and toward a goal (Miller and Cohen, 2001) such as solving a problem, carrying out a predetermined plan (Unterrainer et al., 2004) or optimizing performance on a task. Remembering a phone number for a few minutes would require such control given the continual updating of information in working memory (Shiffrin and Schneider, 1977). This control might go against an automated process (Shallice, 1988) (e.g., inhibition of an automated response such as reading a word in a Stroop task, where one must instead name the color of the ink with which the word is written).
Several studies conceive EF as a "fractionable" concept (Alvarez and Emory, 2006), which relies on distinct cognitive mechanisms of attentional control. EF can be conceptualized as a three-factor model (Friedman and Miyake, 2017) that includes switching (local and global), inhibition, and working-memory updating. Some studies suggest that the subcomponents of EF are affected differently by aging. For instance, updating tends to be less affected by age compared with inhibition and switching (Maldonado et al., 2020). Moreover, in normal aging, the decline in global switching (e.g., the cost of maintaining and coordinating two tasks relative to performing a single task) is known to be more pronounced and to occur earlier compared to local switching (e.g., the cost associated with alternating between two tasks) (Kray and Lindenberger, 2000;Wasylyshyn et al., 2011;Kray and Dörrenbächer, 2019). AsVO 2 max has been shown to decrease with age (Hawkins and Wiswell, 2003), many studies have investigated its relation to EF in older adults. Understanding the relationship between CRF and EFs is of particular interest, as they are known to predict older adults' ability to maintain independent living (Vaughan and Giovanello, 2010) and has been associated with many behaviors that affect daily life, including driving (Anstey et al., 2005), walking (Mirelman et al., 2012), medication adherence (Insel et al., 2006), urinary incontinence (Lussier et al., 2013), and use of problem-solving strategies (Taconnat and Lemaire, 2014). EF was associated with the Instrumental Activities of Daily Living (IADL) scale (Cahn-Weiner et al., 2002) and was associated with a measure of life expectancy and quality of life (Quality Adjusted Life Year) (Davis et al., 2010) in older women.
Association has been reported in older adults between EF and GXT CRFe (van Boxtel et al., 1997;Barnes et al., 2003;Brown et al., 2010;Verstynen et al., 2012;Weinstein et al., 2012;Berryman et al., 2013;Albinet et al., 2014;Freudenberger et al., 2016). Moreover, an association between a z-composite score combining the CRFe provided by the ROCKPORT and a GXT performed on a treadmill was found with EF (Prakash et al., 2011). Performance on the ROCKPORT was also directly associated with inhibition as measured by a flanker task (Colcombe et al., 2004). In a study assessing physical activity levels through questionnaires and accelerometers, CRF with the ROCKPORT and EF with a three-factor model (Miyake et al., 2000) (but only using a global switching task), only inhibition was associated with physical activity . As CRF was not measured by GXT, the lack of association between performance on the global switching task and CRFe could have been explained by the choice of CRFe. To our knowledge, no study has used all types of CRF estimation to assess potential associations with EF, and few studies have examined all subcomponents of EF. To address this shortcoming, the objective of this study was to assess in older adults, CRFe (GXT, ROCKPORT, EQUATION) and their association with EF [switching (both local and global), inhibition, and workingmemory updating]. Furthermore, to minimize the task impurity that characterized EF task (Friedman and Miyake, 2017) and to reduce the effect of processing speed on EF , performance for each EF was computed in terms of different costs calculated in percentages. We hypothesized that the EF subcomponents (inhibition and global switching) known to decline the most during aging would be strongly associated with the most precise CRFe (GXT and, to a lesser degree, ROCKPORT). No significant association is expected between EF subcomponents that are known to be less altered during aging such as updating and local switching.

Experimental Procedures
As a screening procedure to optimize participants' safety, each participant was assessed by a geriatrician before CRF testing. The CRF tests [GXT, ROCKPORT, and EQUATION (Jurca et al., 2005)] were conducted on two non-consecutive days (7.15 ± 1.66 days apart). On the first day of the assessment, participants completed a neuropsychological and a geriatric assessment, followed by a GXT. On the second day, participants completed a questionnaire assessing their habitual physical activity levels (Self-Reported Physical Activity) to complete the EQUATION, and then proceed with the ROCKPORT.

Participants
This study was approved by the Ethics Committee of the geriatric institution where it was conducted and was part of a larger clinical trial (NCT02455258). Informed consent was obtained from all participants. Participants were recruited from the community through advertisements in newspapers, magazines dedicated to the older adults, flyers, websites, and through the research center's pool of participants. To be included, participants had to be sedentary (less than 150 min of moderate-intensity exercise per week) and at least 60 years old. Exclusion criteria were the use of a walking aid or hormone therapy, having undergone surgery under general anesthesia, participation in a structured physical activity program within the past 6 months, smoking within the past 5 years, having been diagnosed and/or treated for major depression or uncontrolled medical conditions such as neurological disorders and cardiorespiratory diseases, and presenting significant or uncorrected perceptual limitations or significant cognitive impairment [as determined by a cutoff score of 26 on the Mini-Mental State Examination (Folstein et al., 1975)]. Table 1 presents the characteristics of the participants (n = 60, mean age 68 years, 77% women).

Assessment of Cardiorespiratory Fitness
Under the supervision of a geriatrician and a certified kinesiologist, GXT was performed on a cycle ergometer (Corival Recumbent, Lode B.V., Groningen, Netherlands) with a metabolic cart (Moxus, AEI Technologies Inc., Naperville, IL, United States). The full protocol has been described elsewhere (Berryman et al., 2013(Berryman et al., , 2014. The test started at 35W for women and 50W for men. Participants were instructed to maintain a pace between 60 and 80 rpm. Each minute, the power of the ergocycle was increased by 15W. The test ended when the participant was unable to maintain the required pace. Constant positive verbal encouragement was given during the test to ensure maximal effort from the participant. The highestVO 2 over a 30s period was considered asVO 2 peak (in ml.kg −1 .min −1 ). All analyses for the GXT included only participants who achieved a respiratory exchange ratio (RER) greater than 1.1, a value that qualifies participants' effort during the test as excellent (Balady et al., 2010).
For the ROCKPORT, participants were asked to walk one mile (1,609 m) as fast as possible (Fenstermaker et al., 1992) on an indoor track. Participants performed this test alone or in small groups of two to four people. A stopwatch was used to record the time achieved and heart rate was recorded throughout the test by a Polar RS 800 beat-to-beat recorder (Polar Electro Oy, Kempele, Finland). CRF was estimated using an equation that included age, biological sex, body mass, time to complete the ROCKPORT, and post-ROCKPORT heart rate (Kline et al., 1987;Fenstermaker et al., 1992). ROCKPORT CRFe in ml.kg −1 .min −1 = 132.853 -  (Kline et al., 1987). Cardiorespiratory fitness was also estimated with the equation derived by Jurca et al. (2005): EQUATION. This equation was designed to consider sex, age, Body Mass Index, and resting heart rate, as well as self-reported habitual physical activity levels [as assessed by a Self-Reported Physical Activity questionnaire (SRPA)]. EQUATION CRFe in ml.kg −1 .min −1 = [Sex (Men = 1, Women = 0)] × (2.77) -Age × (0.10) -Body Mass Index × (0.17) -Resting Heart Rate × (0.03) + SRPA + 18.07. The SRPA categorizes the participant's self-reported level of physical activity. Category 1: Inactive or low levels of physical activity other than usual daily activities (value = 0). Category 2: Regular (≥5 days/week) participation in physical activities for at least 10 min at a time and requiring low level of exertion (value = 1). Category 3: Engagement in aerobic exercises at a comfortable pace for 20-60 min per week (value = 2). Category 4: Participation in aerobic exercises at a comfortable pace for 1-3 h per week (value = 3). Category 5: Participation in aerobic exercises at a comfortable pace for over 3 h per week (value = 4).

Assessment of Cognitive Functions
Cognitive testing was performed using a computerized tabletbased EF assessment battery (Lussier et al., 2020), which includes a digit Stroop task (allowing us to examine the EF inhibition, global and local switching subcomponents) and an n-back task (updating). Before each task, detailed instructions appeared on the screen (which were also read aloud), followed by a familiarization block.
The digit Stroop task was modeled after the Five Digit test (de Paula et al., 2017); a digit-Stroop. The Stroop task consisted of four distinct conditions: reading (60 trials), counting (60 trials), inhibition (60 trials), and switching (73 non-switching trials and 48 switching trials). Figure 1 illustrates the Stroop task conditions. Participants were instructed to respond as quickly as possible while minimizing errors by pressing the buttons on either the right or left side of the screen with their thumbs. In the reading condition, participants had to identify the digit (from 1 to 6) displayed on the screen. Up to six digits could be presented simultaneously on the screen. The digits and the quantity of digits were always matched (e.g., digit 2 appearing at two locations). In the counting condition, participants had to report the number of asterisks (up to six asterisks could be presented). In the inhibition condition, participants had to report how many digits (up to six) appeared on the screen. In this condition, the identity of the digits presented on screen was incompatible with the quantity of digits displayed (e.g., the digit "4" appearing at five locations on the screen). In the switching condition, the instructions were the same as in the inhibition condition, except for the trials (i.e., 48 switching trials out of the 121 trials) in which the digits were surrounded by a white frame, indicating that participants had to report the identity of the digit instead of the number of digits presented. For all conditions, two consecutive trials never gave the same answer. Also, the number used as an answer was equiprobable. The n-back task included three conditions: 1-back (4 blocks of 10 trials), 2-back (4 blocks of 10 trials), and 3-back (2 blocks of 10 trials). Figure 2 illustrates some of the trials of the 2-back condition. Participants were asked to indicate whether a nonverbal stimulus displayed on the screen was identical (by pressing the "=" button) or different (by pressing the " =" button) to the one presented n steps earlier in the sequence. The participant had to use his right thumb to enter his answer. Participants were asked to be as accurate as possible and to respond within a 3,000 ms delay. Each block consisted of 40% target and 60% non-target letters. The duration of the inter-stimulus interval was 750 ms. During this period, accuracy feedback was presented based on the participant's last response, such that the pressed button turned green (accurate answer) or red (error). If the participant accuracy was 75% or more in one condition, the following n-back condition was activated. If not, the task ended. Only the performance on the 1-back and the 2-back were analyzed because most participants were unable to perform the 3-back.
Performance on each EF was computed in terms of different costs calculated as a percentage (interference, local switch, global switch, and updating cost) using reaction time (RT). When calculating costs, it is common to compare the more complex condition to a simpler condition to isolate the process that differentiates them. Therefore, to isolate the local switching cost (the cost associated with alternating between two tasks), we used the performance of the switching trials and the non-switching trials. To isolate the global switching cost, the cost of maintaining and coordinating two tasks relative to performing a single task, we used the performance of the non-switching trials and the inhibition trials. The rationale is that both trials share the same visual presentation and instruction, except that in the nonswitching trials the participant has to keep in mind two sets of instruction and prepare themselves accordingly as they don't know in advance which trials (non-switching trials vs. switching trials) will be presented [task uncertainty (Kray et al., 2002)]. To isolate the updating cost, we used the respective performance on the 2-back (which is demanding in terms of updating) and the performance of the 1-back (which barely necessitates updating).
The costs were computed according to standard procedures (Li et al., 2018). Interference cost: [(inhibition trial RT -counting trial RT) / counting trial RT], local switching cost: (switching trial RT -non-switching trial RT) / non-switching trial RT, global switching cost: (non-switching trial RT -inhibition trial RT) / inhibition trial RT, and updating cost: (n-back 2 trial RTn-back 1 trial RT) / n-back 1 trial RT. Only the RTs associated with a correct response were included in the computation of the respective cost. Maximum RT of 4,000 ms and a minimum RT of 200 ms were allowed. Table 1 shows participants' cognitive data.

Statistical Analysis
The normality assumption was tested using the Kolmogorov-Smirnov test and the Shapiro-Wilk test. The CRFe on the ergocycle was determined as the reference CRFe in the agreement analysis. Systematic bias was assessed using a paired t-test. The magnitude of the difference (Cohen, 1988) was classified using the effect size approach: small (0.2), moderate (0.5), and large (0.8). A regression analysis of the differences was computed to assess proportional bias (Bland and Altman, 1999). Pearson's correlation coefficients (r) were also computed to assess linear relationship between each estimate. The strength of the associations was interpreted as very weak (0-0.19), weak (0.2-0.39), moderate (0.40-0.59), strong (0.6-0.79), and very strong (0.8-1) (Campbell and Swinscow, 2009). Fisher r-to-z transformations were calculated to assess whether there were significant differences between the correlation coefficients.
To assess the relationship between CRFe and EF, two-stage multiple hierarchical regression analyses were performed. Age and education were entered in the first block. In addition to age and education, CRF scores (1 analysis for each estimate) were entered in the second block. Dependent variables were the interference cost, the local switching cost, the global switching cost, and the updating cost. All analyses were performed using Statistical Package for the Social Sciences 27.0 (SPSS Inc., Chicago, IL, United States) and statistical significance was set at p < 0.05.

Cardiorespiratory Fitness Estimates Agreement Analysis
On the basis of the RER criterion (≥1.1), seven participants were excluded from GXT-specific analyses. Seven participants (including one overlapping with the RER criterion) were also excluded from the ROCKPORT-specific analyses because their CRFe were deemed to be too low. These participants' CRFe values were below a cutoff (10.8 ml.kg −1 .min −1 ) determined by previously collectedVO 2 peak data from a GXT performed on a cycle ergometer in a similar sample of 59 healthy older adult participants.
A strong positive correlation was found between GXT and EQUATION; r(51) = 0.64, p < 0.01. A moderate positive correlation was observed between GXT and ROCKPORT; r(45) = 0.58, p < 0.01, and the EQUATION and the ROCKPORT, r(51) = 0.52, p < 0.01. Using Fisher r-to-z transformation, no difference was found between correlation coefficients.

Relation Between Cardiorespiratory Fitness Estimates and Executive Function
Because the analysis included fewer than five independent variables, a sample size of 60 was considered adequate (Harris, 1985). The assumption of singularity was met, as no independent variable correlated with another at 0.70 or greater. Hierarchical regressions were calculated to predict EF costs based on Age and Education (Model 1) and Age, Education, and a CRFe (Model 2). Note that Age is included in the computation of ROCKPORT and EQUATION. Significant associations were observed between Age and GXT (−0.32) and Age and ROCKPORT (−0.47), whereas no significant association was found between Age and the EQUATION. Tolerance (>0.2) and VIF (<10) were all within acceptable limits. Mahalanobis distance scores indicated no multivariate outliers. Table 2 presents the summary results of the hierarchical regressions.

DISCUSSION
The purpose of this study was to examine the associations between three CRFe (GXT, ROCKPORT, EQUATION) and EF (switching, inhibition, and working-memory updating) in healthy older adults. All CRFe were associated with the interference cost. We found significant negative relationships between the global switching cost and the GXT as well as the ROCKPORT. This suggests that exercise-based protocols, such as GXT and ROCKPORT, appear to be better predictors of the relationships between CRF and EF, as they yielded consistent results for the different EF subcomponents. While the variances explained by each CRFe are statistically significant, they remain small and should be interpreted with caution in terms of clinical significance. No significant relation was observed between the global switching cost and EQUATION. Such a discrepancy could stem from the inherent challenge of self-reporting physical activity levels (Sylvia et al., 2014), a variable included in the EQUATION. No relationship was observed between the three CRFe and the local switching costs or the updating costs. It has been reported that global switching is more sensitive to agerelated decline compared to local switching (Verhaeghen and Cerella, 2002;Wasylyshyn et al., 2011;Ferguson et al., 2021). Therefore, it is possible that similarly to updating, local switching is less sensitive to age-related decline in CRF.
Although our study highlighted the diversity of EFs, as not all EFs subcomponents are related to CRFe, we cannot reject the possibility that the associations between GXT and ROCKPORT CRFe and the global switching and interference cost, are related to shared cognitive processes. This would be compatible with the proposal that the inhibition function depends on more fundamental processes (i.e., goal maintenance and management) that are shared with other EFs (Friedman et al., 2008(Friedman et al., , 2011, and in the case of the present study, particularly with global switching. Correlation coefficients between the three CRFe (characterized as moderate to strong) were similar to previous studies (Mailey et al., 2010;McAuley et al., 2011), suggesting that the three CRFe are equivalent. However, in our study, we detect the presence of a systematic bias. The EQUATION and the ROCKPORT estimates, respectively, overestimated and underestimated the GXT score. Previous studies have also reported overestimation of CRF by the EQUATION (McAuley et al., 2011) and underestimation of CRF by the ROCKPORT (Fenstermaker et al., 1992;Warren et al., 1993) when compared to a GXT performed on a treadmill. Despite these differences in agreement between each CRFe, similar negative associations were found between each CRFe and interference cost. Our findings also suggest that education is a significant predictor of interference cost in the model that included GXT or ROCKPORT. Further research in older adults is needed to explain how educational attainment (regardless of the effect of age) might affect a specific cognitive domain (Boller et al., 2017).
Based on our results, CRFe explains a small percentage of variance in EF performance in older adults. Further research is needed to explain how this occurs and how CRF interacts with other mechanisms associated with brain health. For example, the cerebral circulation hypothesis (Rogers et al., 1990) suggests a better vascularization of the frontal lobes. This would benefit performance in tasks requiring EF, as they depend on the integrity of the subcortical frontal circuit, which includes the prefrontal cortex (Gunning-Dixon and Raz, 2003;Alvarez and Emory, 2006). The hypothesis of neurotrophic stimulation (Spirduso, 1980) proposed that the benefit could be related to the production of growth factors, such as Brain-derived neurotrophic factor (BDNF; Szuhany et al., 2015) and Insulin-like growth factor 1 (IGF-1; Cheng et al., 2000) that affect brain metabolism. Other hypotheses also point to preserved vessel elasticity (Gauthier et al., 2015), highlight the importance of inflammation-related markers (Cotman et al., 2007) and their impacts namely on sarcopenia and muscle strength (Schaap et al., 2006), or posit that dopaminergic neurotransmission (Backman et al., 2010) possibly changes after physical exercise (Petzinger et al., 2015).
An important limitation of this study is the small sample size, which limits the number of predictors we could use. Also, because the sample was homogeneous, the potential effect of biological sex could not be analyzed. Furthermore, the cross-sectional design does not allow conclusions to be drawn about the causality of the relationship between CRFe and EF. Another limitation of our study is that the use of beta blockers, potentially related to a reduction in CRF (Nielen et al., 2019), was not evaluated.
One of the strengths of the present study lies in the detailed analyses of the respective EF. From the creation of the tasks   to the cost computation, initiatives were taken to isolate agerelated declines in EF from a more general decline in processing speed. The use of a computerized assessment may be more sensitive to detect differences in EF in a sample of healthy older adults than traditional neuropsychological assessment (Erickson et al., 2019). This study is innovative in measuring three different CRFe in the same sample of older adults, including a GXT performed on a cycle ergometer. Estimation of CRF during a GXT performed on a cycle ergometer has been reported in numerous intervention studies (Blumenthal et al., 1991;Fabre et al., 2002;Jonasson et al., 2016) and offers many advantages in terms of cost, space, portability and ease of simultaneous measurement of other physiological measures (Smith et al., 2016). Indeed, a test without weight-bearing (Huggett et al., 2005) that does not depend upon the participants' walking ability, especially on a treadmill, might be easier to implement in frail older adults, populations with balance issues, increased risks of falls, orthopedic restrictions (Beltz et al., 2016), joint pain or poor lower limb control (e.g., after a stroke) (Tang et al., 2013). This study expands our knowledge about the relationship between CRFe and cognitive functions. We examined whether different CRFe were similarly associated with each EF subcomponent in healthy older adults. Comparable relations were observed between the GXT and ROCKPORT estimates with each EF subcomponents. The absence of a relation between the EQUATION and global switch cost indicates that exercise-based protocols might be more suitable in assessing the relationship between CRF and cognition in older adults The EF subcomponent that was associated with CRFe as measured by the GXT and ROCKPORT, are those known to be the most affected by aging (Maldonado et al., 2020). In their meta-analysis of cross-sectional studies, Maldonado et al. (2020) primarily computed EF performance in terms of RTs or accuracies for a single task, which may limit their ability to isolate EF performance from a more general age-related change in processing speed. Although the use of a difference score computation used in the present study may reduce the likelihood that these associations reflect age differences in processing speed, it cannot exclude it. Therefore, our results should also be interpreted with caution.
Aging is a multifactorial process, however, growing evidence shows that age-related changes in EF performance are related to changes in the integrity of several brain regions (Milham et al., 2002;Jolly et al., 2017), including the prefrontal cortex (Allen et al., 2005;Lemaitre et al., 2005;Coubard et al., 2011). In cross-sectional studies, higher CRF was associated with larger hippocampal volume (Erickson et al., 2009) and had a protective effect on white matter integrity (Marks et al., 2007;Johnson et al., 2012). As CRF is a modifiable factor, improving CRF through the adoption of an active lifestyle could possibly mitigate the brain from age-related insult. The same conclusion could be applied to a reduction of cerebrovascular risk factors. In a recent study, Veldsman et al. (2020) report that in older adults, cerebrovascular risk factors were associated with reduced integrity of the frontoparietal network that affects EF.
Future studies assessing the associations between CRFe and EF subcomponents (particularly inhibition and global switching costs as assessed in the present study) could benefit from the use of neuroimaging techniques such as fMRI (Nashiro et al., 2018), functional near-infrared spectroscopy (Lague-Beauvais et al., 2013;Mekari et al., 2019) and electroencephalography (Gajewski and Falkenstein, 2015b;Gajewski et al., 2018). We also encourage investigators to examine the association between cognitive function (particularly EF) and CRFe in clinical populations, such as people living with mild cognitive impairment (Stuckenschneider et al., 2018), as they are known to present global switching impairment (Belleville et al., 2008). The present results support the promotion of the use of exercise-based protocols to assess CRF when possible and the inclusion of at least one task per EF subcomponents. We also recommended the standardization of the cognitive test batteries used (Voss et al., 2019), since it would improve the comparability between studies from different research groups.

DATA AVAILABILITY STATEMENT
The data from this study, including anonymized participantlevel and study-level data (analyzable data sets) and other information (such as protocols), will be shared with qualified researchers as necessary for conducting legitimate research through a direct request from the study principal investigator (LB at louis.bherer@umontreal.ca).

ETHICS STATEMENT
The participants provided their written informed consent to participate in this study. The study protocol was approved by the Research Ethics Board of the Institut Universitaire de Gériatrie de Montréal (IUGM). The study was part of a larger clinical trial (NCT02455258).

AUTHOR CONTRIBUTIONS
LB conceived the grant application to the Quebec Ministry of Health and Social Services. DP contributed to the study design and provided the first draft of the manuscript. ML, NB, and LB actively contributed to manuscript writing and statistical analyses. DP, NB, FC, TV, and JV tested the participants. All authors played a specific role in the study based on their expertise, either by providing advice, measurement tools, and expertise.