The Heterogeneity of Disruptive Behavior Disorders – Implications for Neurobiological Research and Treatment

Disruptive behavior disorders (DBDs) are reflected by a great variety of symptoms ranging from impulsive-hot-tempered quarrels to purposeful and goal-directed acts of cruelty. A growing body of data indicates that there are neurobiological factors that increase the risk for developing DBDs. In this review, we give a broad overview of recent studies investigating physiological, neural, genetic factors, and specific neurotransmitter systems. We also discuss the impact of psychosocial risk and consider the effects of gene-environment interactions. Due to the heterogeneity of DBDs, it is concluded that specific subtypes of disruptive behavior should be considered both in terms their biological basis and in regard to specific treatment needs.

Reactive aggression is triggered by a frustrating or threatening event and is often accompanied by anger. The aggressive act is initiated without regard for any potential goal (e.g., gaining the victim's possessions). In contrast, proactive aggression is purposeful and goal-directed (e.g., to obtain the victim's possessions, not usually to inflict pain) and involves a minimal level of physiological arousal. Since proactive aggression is driven by the anticipation of reward, it is under the control of positive reinforcement, either directly or vicariously (i.e., through modeling). Reactive aggression is instead under the control of negative reinforcement, when successful in turning off the provocation of the threat that triggered aggression in the first place (Vitaro and Brendgen, 2005).
This two-factor model has been criticized, mainly due to some difficulty in characterizing the nature of specific human aggressive episodes (Bushman and Anderson, 2001). However, considering that aggressive subtypes are not mutually exclusive, a considerable amount of data supports the existence of two relatively separable populations of aggressive individuals: individuals who present with mostly reactive aggression and those who present with high levels of mostly proactive and some reactive aggression: Besides exploratory and confirmatory factor analyses (e.g., Baker et al., 2008) further evidence supporting the distinction between reactive and proactive aggression has been provided by studies showing different correlates of the two forms of aggression. Reactive aggression is related to earlier physical abuse, early onset of attention problems and impulsiveness (Dodge et al., 1997;Barry et al., 2007), peer rejection or victimization (Lamarche et al., 2007;Salmivalli and Helteenvuori, 2007). In contrast, proactive aggression is linked to highly heritable psychopathy-like callous-unemotional traits  and later delinquent behavior (Vitaro et al., 1998;Brendgen et al., 2001).

OPPOsiTiONal deFiaNT disOrder (Odd)
Oppositional Defiant Disorder (ODD) consists of a pattern of negativistic, hostile, and defiant behavior lasting at least 6 months, during which four (or more) of the following behaviors are present: • Often loses temper • Often argues with adults • Often actively defies or refuses to comply with adults' requests or rules • Often deliberately annoys people • Often blames others for his or her mistakes or misbehavior • Is often touchy or easily annoyed by others • Is often angry and resentful • Is often spiteful or vindictive Each of the above is only considered diagnostic if the behavior occurs more frequently than is typically observed in children of comparable age and developmental level and if the behavior causes clinically significant impairment in social, academic, or occupational functioning.
Oppositional Defiant disorder is not diagnosed if the behaviors occur exclusively during the course of a Psychotic or Mood Disorder or if Conduct Disorder is diagnosed.

CONduCT disOrder
The DSM-IV categorizes conduct disorder behaviors into four main groupings: (a) aggressive conduct that causes or threatens physical harm to other people or animals, (b) non-aggressive conduct that causes property loss or damage, (c) deceitfulness or theft, and (d) serious violations of rules. Conduct Disorder consists of a repetitive and persistent pattern of behaviors in which the basic rights of others or major age-appropriate norms or rules of society are violated.
Typically there would have been three or more of the following behaviors in the past 12 months, with at least one in the past 6 months: aggression to people and animals • often bullies, threatens, or intimidates others • often initiates physical fights • has used a weapon that can cause serious physical harm to others (e.g., a bat, brick, broken bottle, knife, gun) • has been physically cruel to people • has been physically cruel to animals • has stolen while confronting a victim (e.g., mugging, purse snatching, extortion, armed robbery) • has forced someone into sexual activity destruction of property has deliberately engaged in fire setting with the intention of causing serious damage has deliberately destroyed others' property (other than by fire setting) deceitfulness or theft has broken into someone else's house, building, or car often lies to obtain goods or favors or to avoid obligations (i.e., "cons" others) has stolen items of non-trivial value without confronting a victim (e.g., shoplifting, but without breaking and entering; forgery) serious violations of rules often stays out at night despite parental prohibitions, beginning before age 13 years has run away from home overnight at least twice while living in parental or parental surrogate home (or once without returning for a lengthy period) is often truant from school, beginning before age 13 years www.frontiersin.org October 2010 | Volume 1 | Article 21 | 3 Stadler et al.
Disruptive behaviour disorders: neurobiological research as low orienting reaction, accelerated habituation and autonomic hyporesponsiveness to punishment cues are associated with aggressive and antisocial behavior in children and adolescents (for a review see Lorber, 2004;Ortitz and Raine, 2004). Children with low resting heart rate appear to be genetically predisposed toward disruptive behavior problems ). On the other hand, an enhanced heart rate and skin conductance response was found especially in those children exhibiting predominantly reactive aggression (Lorber, 2004). This line of research has further revealed that autonomic hyporesponsiveness is of prognostic value. Autonomic underarousal and low autonomic responses in 15-year-old boys are predictive of adult criminal behavior, particularly in boys without psychosocial disadvantages, while heightened autonomic responsiveness appears to be a protective factor against criminal outcome in high-risk male adolescents (Raine et al., 1990(Raine et al., , 1995(Raine et al., , 1997. Even at the age of 3 years, poor fear conditioning is predictive of future criminal behavior in adulthood (Gao et al., 2010) Herpertz et al. (2001, 2003 have found decreased electrodermal responses and accelerated habituation to orienting and startling tones specific for children with CD, irrespective of comorbid ADHD. In addition, these children also exhibited a generalized deficit in autonomic responsivity when exposed to pictures of unpredictable affective quality (Herpertz et al., 2005).
The finding of low autonomic arousal to negative and aversive stimuli as well as an impaired recognition of emotional cues like facial expression in children with antisocial behavior and callousunemotional traits suggests a link between the disposition of aggression and a failure of emotion processing in this subgroup Loney et al., 2003). The propensity for aggressive behavior may be associated with a low threshold for activating negative emotions like fear, empathy or guilt. The lack of emotional and autonomic responsiveness may result in a failure to develop behavioral strategies to avoid negative consequences, thus affecting adequate social and moral learning processes (Blair, 2006). In addition, the ability to detect an affective state of another person is considered a precursor of empathy. It has been shown that there is a strong inverse correlation between the ability to empathize with the emotional experiences of others and self-reported acts of Proactive forms of aggression seem specific for CD or antisocial behavior disorder, whereas elevated levels of reactive aggression are found in many disorders, e.g., depression, anxiety, bipolar disorders and posttraumatic stress disorders (Blair, 2003).
There is increasing evidence that stability of aggressive behavior varies between subtypes: According to a longitudinal twin study, reactive aggression decreases over time, while proactive aggression remains stable. Longitudinal analyses have indicated that the stable variance in reactive aggression is mainly due to genetic and non-shared environmental influences, whereas the stable individual differences in proactive aggression are primarily genetically mediated . Thus, there is some indication that these subtypes are mediated through different psychopathological pathways and different developmental trajectories (Murray-Close and Strove, 2009). One of the most consistent neurobiological findings is reduced serotonergic functioning, mainly in reactive aggression, and reduced physiological arousal particularly in proactive aggression (Kempes et al., 2005). Hence, the presence of genes associated with deficient serotonergic functioning may predispose a child to angry outbursts of reactive behavior when faced with provocation or threat. In contrast, a biological/genetic predisposition to physiological hyporeactivity may render a child less sensitive to stressors and to the aversive consequences of aggressive behavior (i.e., punishment), thus increasing the risk for proactive aggressive behavior.
To understand the different psychopathological pathways of aggressive and antisocial behavior, it seems also important to investigate those processes which are necessary for social learning. From a neuro-scientific point of view, one should therefore ask which biological and psychosocial factors hamper the development and refinement of control and inhibition processes (e.g., to inhibit hot-tempered impulses), adequate emotion processing (to perceive and interpret emotional cues) or the development of empathy (e. g. perspective taking, to feel with others).

phySiological correlateS
Psychophysiological research has provided substantial evidence suggesting that decreased autonomic baseline arousal, e.g., low resting heart rate and low resting electrodermal activity as well  (Carr and Lutjemeier, 2005). Poor empathic responding, particularly reduced responsiveness to the fear and sadness of others, has been linked to heightened levels of proactive aggression (Blair, 2001). Patrick and Bernat (2009) have reviewed findings on electrocortical response measures (EEG/ERP) in individuals exhibiting aggressive behavior: It has been repeatedly shown that impulsive/ reactive-aggressive individuals show an enhanced cortical slow-wave activity in brain areas including frontal and temporal regions, especially in the delta frequency range (Volavka, 1990). Furthermore, particularly among individuals exhibiting impulsive aggression, a reduced amplitude of the P300 response is a consistent finding. Although, a reduced P300 appears to be a general indicator of externalizing behavior characteristic for various disorders, indicating a dispositional vulnerability to impulse control problems (Krueger et al., 2002) it has been proposed that this electrophysiological pattern differs from proactive aggression and callous-unemotional traits (Patrick et al., 1997;Frick and White, 2008).

Structural and functional brain correlateS
Emotion processing in the human brain involves a complex network of brain regions including the amygdala, orbital frontal cortex, cingulate cortex, insular cortex, and other interconnected regions (Dolan, 2002).
In adults with aggressive and antisocial behavior, a number of brain imaging studies have demonstrated structural abnormalities in these brain regions (for a review see Plodowski et al., 2009). Prefrontal gray matter has repeatedly been found to be reduced in adult antisocial individuals and is inversely related to psychopathic behavior (Raine et al., 2000;Yang et al., 2005). Interestingly, the study by Yang et al. (2005) revealed that unsuccessful psychopaths, but not successful psychopaths, had a significant reduction in prefrontal gray matter volume compared with control subjects. The prefrontal structural deficit may underlie low autonomic arousal, poor fear conditioning and reduced stress responsivity in antisocial, psychopathic behavior (Raine et al., 2000;Kruesi et al., 2004). Furthermore, poor fear conditioning is hypothesized to be associated with poor conscience development, as individuals autonomically less responsive to aversive stimuli seem to be unsusceptible to socializing punishment (Raine, 1993).
There is now also evidence for functional and structural brain abnormalities in youths with CD. One study reported a reduction of gray matter volume in right temporal lobe in CD compared to control subjects (Kruesi et al., 2004). Prefrontal volumes in subjects with CD were 16% smaller than in controls, but the difference did not reach statistical significance. Two recent studies used a voxel-wise method (voxel-based morphometry) to compare gray matter volume CD patients and age-, sex-, and intelligencematched control subjects (Sterzer et al., 2007;Huebner et al., 2008). Both studies reported decreased gray matter volume in mesial temporal lobe structures, including the amygdala, and a significant association of these gray matter deficits with the severity of conduct problems. Orbitofrontal gray matter volume was also found to be reduced , in line with previous reports of conduct problems in individuals with early acquired prefrontal lobe damage (Anderson et al., 1999). Sterzer et al. (2007) additionally found a gray matter volume reduction in bilateral anterior insular cortex, which correlated with empathy levels as assessed behaviorally. This finding supports the notion of a crucial involvement of the anterior insula in the experience of empathy (De Vignemont and Singer, 2006). A recent VBM study in a non-clinical sample of boys with conduct problems and callous-unemotional traits reported increased instead of decreased gray matter in orbitofrontal and anterior cingulate cortices . This finding was interpreted as a delay in cortical maturation, as the physiological prefrontal age-related gray matter decrease found in normally developing children was not observed in the group of callous-unemotional boys. Despite the obvious differences in sample characteristics between the study by De Brito et al. (2009) and the other two VBM studies by Sterzer et al. (2007) and Huebner et al. (2008), the seemingly opposing results of these studies call for research relating structural brain changes to specific subtypes of aggressive behavior and to potential abnormalities in brain development (Moffitt et al., 2008).
Functional magnetic resonance imaging (fMRI) studies have examined the neural correlates of emotion processing in aggressive and antisocial individuals. In adult psychopaths, functional abnormalities in the amygdala, the OFC, and the anterior cingulate cortex (ACC) have been shown (Kiehl et al., 2001;Veit et al., 2002). The first study on emotion processing in aggressive children and adolescents investigated neural processing of negative affective pictures in a group of boys with CD (Sterzer et al., 2005). This study demonstrated a negative relationship between aggressive behavior and the responsiveness of the left amygdala to strong negative affective pictures. A positive correlation was found with symptoms of anxiety and depression, which are often comorbid features in CD . In low-anxious adolescents, however, the reduced responsiveness of the amygdala seems to be a specific characteristic reflecting a neural impairment in the recognition of emotional stimuli as an early substrate of pathological aggression. Whereas a recent study by Herpertz et al. (2008) showed contradictory results, the notion of amygdala hyporeactivity in youths with high levels of aggression was further supported by two fMRI studies by Marsh et al. (2008) and Jones et al. (2009), who found reduced right sided amygdala activation to fearful expressions in children with aggressive conduct problems and callous-unemotional traits compared to children with ADHD and healthy controls. In sum, these results indicate that amygdala hyporesponsiveness might be specifically associated with unemotional, low-anxious coldtempered forms of aggressive behavior. As amygdala function is an important determinant of social interactions at the perceptual level, a reduced responsiveness to affective stimuli is in keeping with current models regarding the mechanisms that underlie deficient social interactions and pathological aggression (Davidson et al., 2000;Dolan, 2002;Blair, 2005).
In addition, viewing of affective pictures was accompanied by a pronounced deactivation in the right dorsal ACC in CD patients compared to controls (Sterzer et al., 2005). This deactivation could reflect an affect-evoked suppression of neural activity during emotional exposure, possibly interfering with cognitive functions that might be crucial in the regulation of emotional behavior. In a follow-up study, the authors probed the relationship between the observed functional neural deficits in ACC and Pharmacological challenge studies that assess 5-HT function by measuring the prolactine PRL) response to the 5-HT release/ reuptake inhibitor fenfluramine (FEN) have yielded contradicting results in children and adolescents: The PRL-FEN response was found to be significantly enhanced in aggressive boys (Halperin et al., 1994), differed between aggressive and non-aggressive boys of young age only (Halperin et al., 1997;Pine et al., 1997), or was found to be unrelated to aggression (Schulz et al., 2001). Those studies detecting a positive correlation of 5-HT with aggression were the ones with the youngest samples (Halperin et al., 1994;Pine et al., 1997). Age may thus be an important factor in explaining these inconsistent results and aggressive behavior may be associated with developmental abnormalities of the 5-HT system: Younger children, who initially have an enhanced prolactin response to fenfluramine probably fail to undergo normal developmental changes in 5-HT function and subsequently have a blunted response (Halperin et al., 1997).
Together, although there are still contradictory results in regard to serotonin functioning, there is evidence to suggest that serotonin is involved, at least in part, in mediating reactive-aggressive components of conduct-disordered behavior. Whereas the inhibition of reactive-aggressive behavior might be more strongly associated with serotonergic processes, other neurotransmitter systems, especially norepinephrine, seem to play a role in other subtypes of aggressive behavior, e.g., proactive aggression. Norepinephrine is involved in mediating the impact of aversive cues in human choice behavior (Rogers et al., 2004a,b). This suggests that reduced levels of norepinephrine, leading to reduced responsiveness to aversive cues, might be related to a heightened risk for proactive aggression. In line with this suggestion, there have been a series of reports suggesting that antisocial behavior is associated with reduced norepinephrine levels (Raine, 1993).
In conclusion, although there are still contradictory results, there is some neurobiological evidence pointing toward a role for the serotonin and/or norepinephrine system in CD. Based on the results discussed above, however, future studies should focus on well-defined subgroups of aggressive individuals (i.e., reactive and proactive aggression) in order to explore the role of neurotransmitters in pathological aggression more closely.

neuroendocrine correlateS
Hypothalamic-pituitary adrenal (HPA) axis function is an important determinant of interindividual differences in aggressive and antisocial behavior (Van Goozen and Fairchild, 2009). Besides genetic factors involved in the modulation of the HPA-axis reactivity (Wüst et al., 2000;DeRijk et al., 2006), early stress factors (maternal prenatal smoking, maltreatment) might have -due to the brain plasticity of the developing child -an important influence on HPA-axis activity.
Although there are more contradictory results in children and adolescents compared to antisocial adults, it seems that both basal cortisol and cortisol levels during stress could be valuable biological markers of DBD, whereby cortisol levels are inversely related to aggressive behavior (Vanyukov et al., 1993;Van Goozen et al., 1998b, 2000McBurnett et al., 2000;Pajer et al., 2001;Snoek et al., 2004;Gordis et al., 2006;Pompa et al., 2006;Fairchild et al., 2008). In two longitudinal studies it was shown that a low basal cortisol level specific temperament factors predisposing to DBDs (Stadler et al., 2006). Interestingly, the temperament dimension "novelty seeking" , which comprises high impulsivity and a quicktempered personality, was a significant predictor for ACC responsiveness to affective pictures. Individuals with high novelty seeking were characterized by deficient behavioral control strategies and a lower level of sociomoral reasoning. The deficient activation in the dorsal ACC of patients with CD might hence be a linking factor between temperament factors, cognitive and emotional processing, and behavioral outcome. In that sense, a close relationship between high novelty seeking and an impaired capacity to cognitively control emotional behavior might underlie the predisposition for the development of DBDs.
Taken together, structural brain imaging and fMRI studies on emotion processing in pathological aggression to date suggest that the disposition to aggressive behavior might originate from an impairment of both the recognition of emotional stimuli and the cognitive control of emotional behavior.

neurotranSmitter SyStemS
Serotonin (5-hydroxytryptamine, 5-HT) is thought to be one of the most essential central neurotransmitters to inhibit aggression directed against oneself and others. While a wealth of studies in adult and animal research consistently indicate an inverse relationship between central serotonin and indices of aggression and, more specifically, with impulsive behavior and reactive aggression (i.e., Virkunnen et al., 1995), studies in children have yielded conflicting results. Both direct central and indirect peripheral measures of central serotonergic function have been assessed in children with DBDs. In accord with the findings in adults, an inverse correlation between 5-hydroxyindol acetic acid (5-HIAA) in samples of lumbar cerebrospinal fluid (CSF) and aggression was found (Kruesi et al., 1990). Some studies further indicate that low CSF 5-HIAA correlate with poor outcome (Kruesi et al., 1992) and sociability (Constantino and Murphy, 1996;Clarke et al., 1999). One study reported a positive correlation between CSF-5-HIAA and measures of aggression and impulsivity/hyperactivity (Castellanos et al., 1994), but in less aggressive ADHD patients. A useful alternative method of studying the central 5-HT system in a non-invasive way is the rapid tryptophan depletion test (RTD). The ingestion of a tryptophan-free mixture of amino acids significantly reduces plasma tryptophan, the precursor of serotonin, and as a consequence also central 5-HT levels. Up to now, however, this method has barely been used in children and adolescents. First results indicate an inverse relationship between aggressive behavior and experimentally induced low 5-HT levels  whereas no relationship between executive functioning and altered 5-HT was found (Le Marquand et al., 1998).
In studies of aggressive children using whole blood serotonin assays, peripheral measures of 5-HT yielded mixed results: Some found increased peripheral 5-HT concentrations in DBDs (i.e., Pliszka et al., 1988;Hughes et al., 1996;Unis et al., 1997) or no differences compared to normal or psychiatric controls (i.e., Rogeness et al., 1982;Cook et al., 1995); others reported a negative correlation (Hanna et al., 1995;Van Goozen et al., 1999) or a decrease of platelet serotonin uptake in CD patients (Stadler et al., 2004; for a review see van Goozen and Fairchild, 2009). is predictive of later aggressive behavior. Interestingly, the inverse correlation was more specific for proactive forms of aggression and was not related to affective aggression (McBurnett et al., 2000). This result was recently confirmed by Lopez-Duran et al. (2009) who found higher cortisol reactivity in reactive-aggressive compared to proactive and non-aggressive prepubertal children from a non-clinical sample with an elevated risk for school-age conduct problems. Thus, there is some evidence that increased HPA-axis activity might be specific for the reactive aggression subtype. In line with this hypothesis, Van Goozen et al. (1998b) reported that conduct-disordered children with low levels of anxiety showed a smaller cortisol reaction during a stress situation compared to control children and aggressive children with high levels of anxiety. A positive relationship between anxiety and cortisol levels in children with CD was also found by McBurnett et al. (1991). Thus, low HPAaxis activity seems to indicate fearlessness and thus hyporesponsivity to threatening stimuli (Van Goozen et al., 2000). Accordingly, a recent study showed that children with CD and high callousunemotional traits exhibit low cortisol reactivity compared to CD children with low levels of callous-unemotional traits (Stadler et al., 2010). A meta-analysis conducted by Hawes et al. (2009) supports the prediction that low levels of cortisol are associated with a risk for childhood antisocial behavior, but the relationship is not as strong as assumed. This lack of a strong association may be due to the fact that the relationship between HPA-axis activity and antisocial behavior varies depending on type of aggressive behavior, the presence or absence of callous-unemotional traits, patterns of internalizing comorbidity, and early environmental adversity.
In addition to specific aggression subtypes, one should also take into consideration that different hormones might interact and determine the etiology of aggression simultaneously. Popma et al. (2007) demonstrated that testosterone and aggression were only positively correlated in delinquent boys with low basal cortisol levels whereas there was no relationship between testosterone and aggression in those with high basal cortisol levels. Similarly, Dabbs et al. (1991) found a stronger relationship between testosterone levels and violence in male adolescents with low cortisol levels, compared to those with high cortisol levels. These findings indicate that high cortisol levels might be protective against aggression. Thus, intact cortisol reactivity might buffer the effect of other risk factors by increasing anxiety levels or behavioral inhibition (Kagan et al., 1988). Moreover, Van de Wiel et al. (2004) demonstrated that disruptive children who exhibit low cortisol reactivity profit less from an intervention aimed at decreasing behavioral problems compared to children with intact cortisol reactivity. The knowledge that children with low cortisol reactivity might be at risk for developing aggressive behavior and might be resistant to current intervention programs leads to the necessity to develop new treatment approaches. Patients with an elevated biological risk should perhaps be treated with psychopharmacological means or additive methods aimed at adjusting the biological alterations, before or in combination with psychotherapeutic procedures.
Although several studies demonstrated a positive relationship between testosterone and DBDs (Scerbo and Kolko, 1994;Brooks andReddon, 1996, Gerra et al., 1998), the correlation between aggression and testosterone in humans is less clear-cut than in animals (Archer, 1991;Archer et al., 2005). Some studies failed to find abnormal testosterone levels in children with CD (Van Goozen et al., 1998b;Loney et al., 2006) or in association with callous-unemotional traits (Loney et al., 2006). Van Goozen et al. (1998a) found that adrenal androgens were significantly higher in boys with CD than normal controls and other psychiatric controls (Van Goozen et al., 2000) and were strongly associated with aggression and delinquency (Van Goozen et al., 1998b). In a prospective longitudinal study, disruptive boys had higher levels of the main androgen metabolites testosterone and 5alpha-dihydrotestosterone (Maras et al., 2003).The relationship between testosterone and aggressive behavior seems to be gender-specific and modulated to some extent by comorbid symptoms such as attention problems (Granger et al., 2003). Furthermore, recent findings indicate that testosterone levels are related to social dominance, (Mazur and Booth, 1998;Rowe et al., 2004;Van Bokhoven et al., 2006) or peer-group leadership (Rowe et al., 2004) rather than aggression per se. One further explanation why studies have yielded mixed results (for an overview see also Van Goozen and Fairchild, 2009) may be that the relationship between testosterone and aggression is modulated by genetic factors. Recently, Sjöberg et al. (2008) demonstrated that testosterone levels and monoamine oxidase A (MAOA) genotype interact to predict antisocial behavior. Relative to controls, testosterone levels were elevated only in those patients with antisocial personality disorder expressing the low-activity form of the MAOA.
Although the impact of testosterone on aggressive behavior still is not fully clarified, interestingly, there is evidence indicating that testosterone modulates the processing of emotional stimuli: In humans and non-human animals, testosterone reduces central fear responses and stress axis reactivity (Van Honk et al., 2005;Hermans et al., 2006Hermans et al., , 2007 as well as the aversive responses to unconscious threat stimuli (Wirth and Schultheis, 2007). This lack of perceived threat may make high-testosterone individuals more likely to engage in testosterone-facilitated dominance behavior, and to approach dominance challenges, thus leading to an impaired detection of social signals that serve the correction of behavior and the inhibition of aggression.

genetic factorS
Twin, adoption, and family studies suggest genetic influences on aggression (Miles and Carey, 1997;Rhee and Waldman, 2002), with heritability estimates for dimensional measures of aggression ranging from 44% to 72% in adults. A consideration of specific behavioral or emotion processing components seems to be an effective approach in genetic research: The Twins Early Development Study (TEDS) has shown a remarkably high heritability for callous-unemotional traits (Viding et al., 2005). It is striking that high levels of antisocial behaviors combined with high levels of CU are under strong genetic influence and no influence of shared environment whereas antisocial behavior in children with low levels of CU shows moderate genetic and shared environmental influence.
In addition, behavioral genetic studies have provided evidence that heritable factors are important for both subtypes of aggressive behavior, proactive and reactive aggression Baker et al., 2008). Whereas proactive aggression seems to be predominantly influenced by genetic variables, in reactive aggression genetic as well as non-shared environmental influences seem to contribute to the course and the stability of aggression . Proactive aggressive behavior may thus be related to stable and heritable psychopathic callous-unemotional personality characteristics, which to some extent explains the genetic stability observed in proactive aggression.
Interestingly, advanced approaches combining genetic and neuroimaging studies have yielded evidence that a specific genetic vulnerability might be related to deficient neural processing of emotional stimuli. Mechelli et al. (2009) recently showed that the valine 158 methionine polymorphism is associated with increased gray matter volume and heightened reactivity during emotion processing within the limbic system in children. As this study was conducted in healthy children, further research in DBD is needed. However, these results indicate that genetic factors affect brain function and might thus moderate the vulnerability to affective psychopathology in childhood.
Since CD is a heterogeneous disorder that becomes manifest in various behavioral dimensions and often co-occurs with other child behavioral disorders including comorbid ADHD and emotional disorders, it is likely that different neurotransmitter systems and the relative balance between them have varying degrees of influence over the behavioral phenotype. Variations in genes involved in these neurotransmitter systems are likely to mediate this delicate balance and have an effect on the function of these neurotransmitters in the brain.
In addition, it seems that the influence of genetic factors is moderated by environmental factors (Caspi et al., 2002; see also below): A functional polymorphism in the gene encoding the neurotransmitter-metabolizing enzyme MAOA was found to moderate the effect of maltreatment. Maltreated children with a genotype conferring low levels of MAOA expression were at high risk to develop antisocial problems whereas children with high levels of MAOA expression were not. These findings may partly explain why not all victims of maltreatment grow up to victimize others, and they provide epidemiological evidence that genotypes can moderate children's sensitivity to environmental insults.
In humans, length variation of the serotonin (5-HT) transporter (5-HTT) gene-linked polymorphic region (5-HTTLPR) that results in allelic variation in 5-HTT expression is associated with decreased serotonergic function and 5-HT-mediated psychopathology. This specific polymorphism in the serotonin transporter gene is associated with deficits in early behavioral functioning and serotonin metabolism, and extreme aggression among monkeys who experienced insecure early attachment relationships but not in monkeys who developed secure attachment relationships with their mothers during infancy (Suomi, 2005).
Additional animal studies have suggested that specific serotonin receptors may be involved in impulsive behaviors. Investigations with knockout mice that lack the 5-HT1B receptor show increased cocaine acquisition and alcohol intake, as well as hyperactivity and aggressive behavior (Brunner et al., 1999).

early health factorS, pSychoSocial factorS and gene-environment-interaction
Early health factors as well as several psychosocial risk factors increase the probability for CD. For example, birth complications may impair brain development and lead to a dysregulation in biological processes that can ultimately result in aggressive and antisocial behavior (Raine, 2002a,b). One important factor which is assumed to be associated with deficient brain development is maternal smoking during pregnancy. Prenatal nicotine disrupts the development of the noradrenergic neurotransmitter system (Levin et al., 1996) and interferes with neural development and autonomic functioning.
Besides health factors, psychosocial and parental factors have to be considered. A child's risk of developing CD is increased by parent psychopathology: Maternal depression, paternal alcoholism and/or criminality and antisocial behavior in either parent (Pfiffner et al., 2005;Kopp and Beauchaine, 2007) have been specifically linked to DBDs. Inconsistent parenting, higher levels of punishment with a concurrent reduction in reasoning and rewards, parents' negative perception of their child's adjustment, and reduced parental monitoring are additional determinants of DBDs and, most notably, seem to predict high probability of transition from ADHD to ODD and CD (Patterson et al., 2000). There is some evidence that variables presumed environmental in the first instance (e.g., parenting) may also reflect underlying genetic vulnerability within families. For example, at least part of the relationship between parenting and child behavior might reflect parental response to the child's genetically influenced temperament: Larsson et al. (2008) revealed an underlying bidirectional process involved in parental negativity and childhood antisocial behavior. On the one hand, negative parenting environmentally mediates the risk for child antisocial behavior; on the other hand, Larsson et al. (2008) also showed a strong impact of genetically influenced antisocial behavior on parenting.
Life stressors such as poverty, unemployment, low socioeconomic status, and affiliation to an ethnic minority (McCabe et al., 2001) are known to have an adverse effect on parenting and are therefore also related to the development of DBDs. In general, the impact of psychosocial factors seems to be more relevant for disruptive behavior starting early in child development (McCabe et al., 2001). Loeber et al. (2005) showed that the proportion of boys committing violent offences linearly increases as a function of the number of risk factors: The probability of future violence ranges from 3% in the absence of risk factors to 100% with nine or more risk factors. The 11 strongest predictors were the following: truancy, low school motivation, onset of severe aggressive conduct problems before age 10, cruelty to people, depressed mood, physical aggression, callous or unemotional behavior, low family socio-economic status, high parental stress, and bad neighborhood. Besides the above-mentioned risk factors, protective factors have to be considered as well: Fergusson and Lynskey (1996) revealed that adolescents seem to be more resilient to externalizing behavior despite high exposure to family adversity during childhood when they had higher IQ, lower novelty seeking and lower affiliations with delinquent peers, thus showing a combination of favorable intellectual ability, temperamental characteristics, and wider environmental factors.
As mentioned above, recent studies also indicate that the impact of psychosocial factors can be influenced by genetic variables. There is some evidence that inconsistent findings in regard to specific genetic variants can be explained by gene-gene or gene-environment interactions. A significant gene-gene interaction was recently confirmed by the Mannheim Study of Children at Risk (Hohmann et al., 2009), indicating that genes in the dopamine and serotonin systems are related to externalizing behavior and that both interact: Adolescents carrying two copies of the 5-HTTLPR short allele and the DRD4 7r variant scored highest on aggressive and/or delinquent behavior compared to other genotypes.
In addition, there is mounting evidence demonstrating the interaction between early risk factors like physical abuse and specific genetic variants, e.g., a functional polymorphism in the promoter region of the MAOA gene (Caspi et al., 2002;Foley et al., 2004;Kim-Cohen et al., 2006). Inconsistent findings regarding the interaction of genetic and early environmental factors (Haberstick et al., 2005;Huizinga et al., 2006) could reflect effects from various sources: In a family study by Thapar et al. (2005) low birth weight and the val alleles of the COMT gene predicted antisocial disorder in children with ADHD. Earlier physical abuse and genetic effects were also found in twin studies in association with conduct problems (Boivin et al., 2005;Jaffee et al., 2005). In addition, maternal hostile-reactive behaviors seem related to genetic factors in the child (see Moffit, 2005) Previous adoption studies show significant genetic influence on delinquent behavior. The highest rate of delinquent behavior was discovered in adopted children with delinquent biological and adoptive parents, and the second highest if only the biological parents but not the adopting caregivers were delinquent (Bohman, 1996). The effect of parental separation (of the adopting parents) and negative emotionality of biological parents (prior to adoption) on the children adopted in early infancy was studied in the Colorado Adoption Project (CAP): Children's externalizing behavior at school and in the family correlated significantly with the negative emotionality in the divorced families compared to the nondivorced (O' Connor et al., 2003). The Twins Early Development Study (TEDS, Trouton et al., 2002) on approximately 3700 twins illustrated a high genetic influence on aggressive behavior when associated with callousness-unemotionality while the impact of environmental factors was weak (Viding et al., 2005). Similar findings were obtained from another large twin sample (Larsson et al., 2006). Viding et al. (2009) furthermore revealed that, contrary to the development of callous-unemotional traits, negative parental discipline operates as a non-shared environmental risk factor for development of conduct problems during the transition to early adolescence.
Studying familial mechanisms, Deater-Deckard et al. (1999) separated genetic parental effects (warmth, acceptance negativity, hostility, withdrawal) from those stemming from the child (low task orientation). The latter contributed more to the genetic variance than familial factors. In later studies on the same group of children (Deater-Deckard and O'Connor, 2000;Deater-Deckard and Petrill, 2004) conduct problems covaried not only with parental negative affect and control as well as with some educational situations but also showed a genetic effect (higher concordance in MZ vs. DZ twins).

temperamental and Social cognitive riSk factorS
It has been proposed that the ability to cognitively control behavior and emotions is determined by various temperament factors . Certain temperamental vulnerabilities can disrupt normal developmental processes during early childhood, such as the development of emotional regulatory abilities.
Temperamental factors can thus place a child at risk for antisocial and aggressive behavior (Robison et al., 2005). Recent evidence shows that reactive aggression in adolescence is predicted by temperamental difficulties in childhood (Vitaro and Brendgen, 2002;Ortitz and Gandara, 2006). More specifically, reactively aggressive preadolescents have shown more negative emotionality (e.g., irritability) during earlier childhood than proactively aggressive or non-aggressive peers (Vitaro and Brendgen, 2002). The risk for the development of aggressive behavior problems is also markedly increased in children and adolescents with high impulsivity/ novelty seeking (Tremblay et al., 1994;Schmeck and Poustka, 2001) and to some extent in adolescents with reduced anxiety or harm avoidance (Sigvardsson et al., 1987). Children with low anxiety are likely to respond less to punishment cues or may represent the subtype of conduct-disordered children with callous-unemotional traits (Christian et al., 1997), predicting an especially severe and chronic pattern of conduct problems and delinquency . As already mentioned, callous-unemotional traits are highly heritable (Viding et al., 2005 and are associated with low cortisol levels (Loney et al., 2006;Stadler et al., 2010) and abnormal fMRI responses to emotional stimuli (Marsh et al., 2008;Jones et al., 2009).
Cumulative and interacting effects of different risk factors have to be kept in mind. Children with a difficult temperament are, for example, at high risk to provoke negative parenting and, as a consequence, harsh parental discipline might negatively contribute to social cognitive processing in the child . Adequate social cognitive processes comprising the encoding of social stimuli, interpretation of cues, clarification of goals, access of adequate behavioral responses are affected in aggressive children. A number of studies have shown that reactively aggressive children misread interpersonal cues and interpreted ambiguous or prosocial communication as hostile, whereas proactive aggressive children mostly show problems concerning the clarification of goals, response access and response decision (Dodge and Coie, 1987;Crick and Dodge, 1996).

treatment
Given the high prevalence and chronicity of DBDs, their effective treatment is a major public health challenge. Psychopharmacotherapeutic approaches for ODD and CD comprise neuroleptics and mood stabilizers and, in ADHD, mostly psychostimulants (for a review see Steiner et al., 2003). Whereas psychosocial treatment approaches theoretically might be effective for both proactive and reactive aggression, psychopharmacology appears effective in the first instance for reactive types of aggression as its effectiveness is mediated by a reduction of impulsivity which is mainly biologically determined (Steiner et al., 2003). Furthermore, psychopharmacological treatment is often limited by side-effects and is usually not effective beyond the treatment period. Nonpharmacologic approaches therefore play an important role in the treatment of DBD.
In general, cognitive-behavioral therapy has been used to alter highly reactive-aggressive behavior. Psychotherapeutic interventions focus on anger management, hostile attribution biases and problem-solving skills. On the other hand, highly proactive aggressive behavior could be influenced by contingency management procedures and social problem-solving skills programs that focus on making aggressive children identify and perceive the negative effect of their aggressive and antisocial behavior. However, accumulating evidence indicates that that the proactive subtype is less treatment sensitive (Vitiello and Stoff, 1997). This might be due to the biologically determined hyporesponsiveness to punishment cues that interferes with contingency management therapy, e. g. the application of negative consequences to aggression.
According to Kazdin (1997Kazdin ( , 2000 three treatment approaches have been well evaluated in the past and show significant evidence: Parent management therapy (PMT), cognitive problem-solving skills training (PSST) and multi-systemic therapy (MST). Parent management therapy commonly uses interventions focused on parenting factors, like coercive social interactions between child and parents or teachers. Major components of parent training programs include: contingency management; increasing parents' ability to monitor and respond appropriately to the child's behavior; clear and concrete and specific goal/target setting; and application of rewards and of appropriate negative consequences. Parent training also focuses on the reduction of parental stress and burden since the interaction between psychosocial adversity and child risk factors further advance the development to severe DBDs.
Whereas cycles of early coercive behaviors in childhood between parent (and teacher) and child are clearly one of the earliest and most powerful antecedents of antisocial behavior, other dysfunctional domains like peer settings become more and more powerful in middle childhood. Children who are oppositional and aggressive toward their parents are at high risk to become involved in aggressive, rejecting relationships with peers. Such relationships accelerate the further development of antisocial behavior (Reid et al., 1999) even beyond specific individual deficits in disruptive children (i.e., poor emotional control and problem-solving skills), and also aggravate internalizing symptoms (e.g., poor self-esteem, depression). As the efficacy of PMT programs declines with increasing age (Barkley, 1997), psychotherapeutic interventions that focus on cognitive processes (e.g., problem-solving skills) are being increasingly applied in middle childhood and adolescence.
This has lead to the development of multi-focused psychosocial programs for child conduct problems (Kazdin et al., 1987;Webster-Stratton et al., 2004). These programs emphasize social skills, problem-solving techniques, and anger management strategies in reducing child aggression and conduct problems, and individual therapy approaches based on the principals of cognitive-behavioral therapy (Durlak et al., 1991).
Multi-focused psychosocial interventions given early in life to at-risk children have most consistently been shown to be effective and sustainable (Webster-Stratton and Taylor, 2001;Farmer et al., 2002;Kazdin, 2006). However, treatments for youngsters with already well-established disorders of aggression are neither robust nor well-established (Connor et al., 2006).
Multi-Systemic Treatment (MST) is an effective intervention that has been developed to treat severely aggressive adolescents with chronic juvenile justice histories (Henggeler and Borduin, 1990;Henggeler et al., 1993;Chamberlain and Reid, 1998;Borduin, 1999). MST focuses on the individual, family, and extra-familial systems and their interrelations as a way to reduce symptoms and to promote prosocial behavior.
It should be noted that there are limitations to the extant treatment programs. Several biological and psychosocial risk factors might influence treatment outcome. Moreover, parental psychopathology may diminish the effectiveness of PMT and family interventions (Beauchaine et al., 2005). Family structures characterized by single parents, younger mothers, homes headed by a non-biological parent or contact with antisocial peers or low child cognitive functions are further adversarial moderators (Connor et al., 2006).
Concerning biological factors, it was shown that autonomically hypo-aroused children might benefit less from a multi-focused intervention program comprising PMT and PSST in a day-care setting (Stadler et al., 2008). In addition, Van de Wiel et al. (2004) showed that disruptive children who are less responsive to stress in terms of cortisol reactivity benefit less from an intervention aimed at decreasing behavioral problems. Thus, for selecting the best possible treatment options, further continued research is needed to examine biological and psychosocial predictors of treatment outcome.
In summary, it can be concluded that parent management training, cognitive-behavioral approaches, problem-solving skill training and multi-systemic approaches are more effective than individual or group psychodynamic or traditional unfocused and open-ended psychotherapy approaches (Kazdin, 1997;Connor et al., 2006). With regard to the high comorbidity with other externalizing and internalizing disorders as well with learning disabilities and associated academic failure, successful intervention also has to focus on comorbid symptoms. Thus, intervention must be multidisciplinary, usually requiring several components of medical, mental health, case management, and educational intervention (Steiner and Dunne, 1997).

diScuSSion and perSpectiveS
A growing body of data indicates that there are neurobiological factors that increase the risk for developing DBDs. It was already outlined that this does not imply an absence of environmental impact (Hinshaw and Lee, 2003;Rutter, 2005). Neuroscience research has to consider the interacting influence of biological and social risk factors, e.g., maltreatment or parental factors. Social factors might increase the risk for the development of CD, but on the other hand there is compelling evidence that protective social factors can counteract a biologically determined vulnerability. As already mentioned, in monkeys a specific polymorphism in the serotonin transporter gene is associated with deficits in early neurobehavioral functioning, serotonin metabolism and aggression only in those monkeys who experienced insecure early attachment relationships but not in monkeys with secure attachment (Suomi, 2005).
Thus, conflicting results from studies in children with disruptive behavior might be due to insufficient consideration of interacting psychosocial factors. Moreover, inconsistencies in the literature are due to the symptom heterogeneity of DBDs and the diversity of behavioral constructs. In addition, contradictory results in neuroscience might also originate from methodological problems like the diversity of studied groups including symptom heterogeneity as well as age differences (Lorber, 2004). A promising approach in investigating biological mechanisms might be the use of experimen-Although our knowledge on neurobiological correlates of disruptive behavior considerably increased in the last years, neurobiological correlates can only be applied at the group level and are far from being specific enough to be used as a diagnostic or screening tool in individuals. Second, due to the immense plasticity of the nervous system, longitudinal studies of neurobiological markers, optimally starting at birth, would greatly help to understand their contribution to long-term behavioral outcome. Importantly, children with an elevated biological risk should not be judged as not capable of change or impervious to intervention or prevention methods. Concerning the application of psychotherapeutic interventions, the most effective method appears to be an integrated approach that considers both the child and the family, within a variety of contexts throughout the developmental stages of the child and family's life. Due to the heterogeneity of DBDs, future research should focus on the investigation of biological and psychosocial correlates of specific subtypes of aggressive behavior with possibly different etiology and specific treatment needs. tal methods for assessing specific types of aggressive behavior. For example, lower affinity of 5-HT uptake in conduct-disordered boys was revealed only in those boys who showed high anger reactivity to an experimentally induced provocation (Stadler et al., 2004). Thus, subclassifications at the symptom level, such as proactive versus reactive aggression, seem to be more helpful in studying underlying biological and neuropsychological mechanisms of DBDs than the use of the diagnostic criteria provided by DSM-IV or ICD-10. Assuming, for example, that serotonergic dysfunction is associated more closely with specific basic psychopathological dimensions like impulsive reactive aggression, it may be beneficial to use methods that assess this particular phenotype and thus distinguish this type from other forms of aggressive behavior which may not be associated with biological markers. Problems associated with the heterogeneity of patient samples could be overcome by using assessment tools that yield -over and above medical taxonomy -empirically derived data specifying the diagnosis CD. Several questionnaires have been developed in order to assess proactive and reactive aggression in children (for a review see Kempes et al., 2005).