LTBI-negative close contacts of tuberculosis are more likely to develop the disease: enlightenment and lessons from a cluster outbreak

Background Tuberculosis (TB) prevention and control among groups living together, such as students, workers, older adults in nursing homes, and prisoners, present many challenges due to their particular age and environmental factors, which can make them more susceptible to TB clusters with significant societal impact. This study aimed to evaluate a TB cluster outbreak epidemic in a university and provide suggestions for improving TB control strategies for groups living together. Methods Pulmonary TB screening and close-contact investigation were conducted using acid-fast staining, sputum culture, GeneXpert testing, tuberculin skin testing (TST), interferon-gamma release assay (IGRA), and chest computed tomography (CT). GraphPad Prism 9.5.1 was utilized for data analysis. Collected epidemic data were comprehensively analyzed by rate comparison. Results The TB cluster outbreak epidemic was identified with an index case confirmed positive. The initial screening was conducted on potential close contacts of the index case, and the TST’s positive rate (diameter ≥ 5 mm) and strong positive rate (diameter ≥ 15 mm) among these close contacts were 65.60% (21/32) and 34.40% (11/32), respectively. Moreover, the latent TB infection (LTBI) rate (diameter ≥ 10 mm) was 43.75% (14/32), and the IGRA’s positive rate was 9.30% (3/32). Chest CT scans did not reveal any abnormalities. Surprisingly, 5 of the close contacts developed active TB in the second screening, accompanied by changes from negative to positive TST and/or IGRA results, after 3 months of follow-up. Accordingly, we expanded the screening scope to include another 28 general contacts. We found that the positive rate (78.00%, 25/32), strong positive rate (50.00%, 16/32), and LTBI rate (62.50%, 20/32) of the 32 close contacts were significantly higher than those of the additional general contacts (28.00%, 8/28; 14.3%, 4/28; 25.00%, 7/28), as indicated by p < 0.05. Conclusion In the event of an epidemic TB outbreak, it is essential to rapidly identify the source of infection and initiate timely screening of close contacts. The initial screening should be focused on individuals without LTBI, who are at higher risk of developing TB. In purified protein derivative-negative individuals living in groups, additional vaccination or revaccination with Bacille Calmette-Guérin may help prevent cluster outbreaks of TB.


Introduction
Tuberculosis (TB) is an old infectious disease caused by Mycobacterium tuberculosis. According to the Global Tuberculosis Report 2022 published by the World Health Organization (WHO) in October 2022, there were 10.6 million new TB cases and 0.96 TB deaths worldwide in 2021 (1). In addition, China has a large population and is among the countries with a high burden of TB, with an estimated 842,000 new cases in 2020, posing a major threat to people's health and livelihoods (2). Furthermore, the COVID-19 pandemic has disrupted access to TB services, resulting in an increase in the incidence and mortality rates of TB, delaying global TB control by approximately 10 years (3). It is reassuring that during the COVID-19 epidemic, various countries explored many effective strategies for the prevention and control of infectious diseases, providing an example for the prevention and control of TB (4).
Bacille Calmette-Guérin (BCG) is the only vaccine for preventing TB. However, it has been reported that the protective effect of BCG vaccination only maintains for 10-15 years (5, 6), indicating that many adolescents over the age of 15 in countries with universal BCG vaccination policies may be at increased risk of M. tuberculosis infection. Interestingly, this age group in China tends to be gregarious university students, the new generation of migrant workers, and young freelancers. In developing countries, individuals between 25 and 44 years are prone to TB (7), which is consistent with a systematic review and meta-analysis (8).
Environmental and host factors are the two major risk factors for TB. Environmental factors determine the possibility of the first infection, whereas host factors determine the probability of postinfection morbidity. University students, the new generation of migrant workers, young freelancers, and prisoners are characterized by collective living, low income, poor living environment, heavy workload, etc. Recently, a study performed in four cities in China quantified the transmissibility of M. tuberculosis among students and non-students and found that TB transmissibility from the non-student to student population strongly influenced students (9). It can be concluded that these particular environmental and host conditions provide fertile ground for the outbreak and epidemic of TB in these populations (9)(10)(11).
In this study, we reported an outbreak of TB at a university in Beijing, China. We identified the individual with active TB (ATB) and conducted two rounds of screening of close contacts using etiologic, molecular, imaging, and immunologic techniques. Our results indicated that five close contacts developed ATB. This study provides a new strategy for preventing and controlling tuberculosis in cluster populations.

Ethic declaration
The study protocol was approved by the Research Ethics Committee of the 8th Medical Center of PLA General Hospital. All procedures followed the Declaration of Helsinki and relevant policies in China. Written informed consent was obtained from all participants before enrolment.

Participants and groups
In May 2021, a male student with ATB was identified at a university in Beijing, China. The diagnosis of ATB was followed by the Tuberculosis Diagnostic Criteria (WS288-2017) formulated by the National Health and Family Planning Commission of China (NHFPC). To avoid the TB pandemic in students, we conducted two rounds of TB screening among close contacts. According to the degree of exposure, we divided all close contacts into two exposure levels: individuals in exposure level 1 are close contacts living in the same room with the index case or good friends of the index case, and individuals in exposure level 2 are close contacts who attended classes in the same classroom with the index case.

Investigation methods
This study examined the index case, including his basic information, onset and consultation, diagnosis and treatment, and close contacts. The close contacts were primarily screened using symptoms, M. tuberculosis acid-fast staining and sputum culture, GeneXpert test, chest computed tomography (CT), tuberculin skin test (TST), and interferon-γ release assay (IGRA). Preventive medication was administered to close contacts with normal chest CT, strong positive TST, and positive IGRA results. After 3 months, an additional screening was performed among close contacts with negative TST and/or negative IGRA results in the first screening. Their positive conversion rate was determined. Individuals whose TST and/or IGRA results became positive on normal chest CT were administered preventive medication. Abnormal chest radiographs and strongly positive TST results were assessed for individuals with suspicious symptoms. TB diagnosis was confirmed using smears prepared thrice, one-time culture, and molecular biological detection using the sputum or bronchoalveolar lavage fluid. Follow-up included medical observation of other screening objects for 6 months ( Figure 1).  Flow chart of the disposal of this cluster outbreak of a TB epidemic.

2.4.
Frontiers in Public Health 04 frontiersin.org spread evenly onto the front of a glass microscope slide, forming an oval sputum film of 10 mm × 20 mm. The slide was allowed to air dry and heat-fixed before staining with the Ziehl-Neelsen method using an M. tuberculosis acid-fast staining kit (product code BA4011, BaSo, Zhuhai, Guangdong province, China), following the manufacturer's instructions. Smear results of 1+ or more were recorded as positive for statistical analysis. Furthermore, the rest of the sputum collected from the close contacts was utilized for sputum culture. In brief, 1-2 times the volume of 4% NaOH was added to the sputum sample, mixed using a vortex mixer, and shaken. After 15-20 min, 0.067 M phosphate buffer solution (PBS) was added and mixed. The sample was then subjected to centrifugation at 3,000 g for 20-30 min to obtain the supernatant, which was removed. The residue was mixed with 0.5 mL of PBS, and 0.1 mL to 0.3 mL of the mixture was inoculated onto a Roche culture tube (product code BA7005C-2, BaSo, Zhuhai, Guangdong province, China) and incubated at 37°C in an incubator. The tubes were observed once on the 3rd and 7th days after inoculation, and subsequently once a week until no colonies were negative at week 8, while colonies appearing positive were recorded.

GeneXpert test
For sputum pretreatment, one mL of sputum sample and 2 mL of 4% NaOH were added to a screw-capped pretreatment tube. The tube was tightly closed and placed in a biosafety cabinet for vortexing for 1 min to ensure complete mixing. After treatment, the samples were allowed to rest at room temperature for 15 min, followed by the addition of 45 mL of 0.067 M PBS to the liquefied sputum. The screw cap was then tightened, and the sample was centrifuged at 3,000 g for 20 min. The supernatant was discarded, and the precipitate was resuspended in 2 mL PBS. Sequentially labeled according to the specimen's serial number, the Xpert MTB/RIF reaction box was used for testing by slowly aspirating 0.5 mL of the sputum precipitate suspension from the well of the reaction box. The lid of the box was then closed, and the GeneXpert XVI (Cepheid AB, Solna, Sweden) was used for testing, following the manufacturer's instructions. After 2 h, the test results were read: (1) M. tuberculosis Ct value less than 16 was considered high; (2) Ct value 16-22 was considered intermediate; (3) Ct value 22-28 was considered low; (4) Ct value greater than 28 was considered very low. A sample with a Ct value less than 28 was reported as M. tuberculosis positive, and a Ct value higher than 28 was declared as M. tuberculosis negative.

IGRA
A total of 5 mL of venous blood was collected from each participant using heparin-anticoagulated vacuum collection tubes, gently inverted to mix the anticoagulant and blood. Three separate culture tubes were prepared: T-tube containing M. tuberculosisspecific antigens ESAT-6 and CFP-10, N-tube as a background control without any antigens, and P-tube as a positive control supplemented with the non-specific stimulating antigen plant hemagglutinin (PHA). One mL of the blood sample prepared above was added to each tube, gently mixed, and incubated for 22 h in an incubator at 37°C. The supernatant was collected after centrifugation at 1,000 g for 10 min.
The IFN-γ concentration was detected by a TB-IGRA Detection Kit (Beijing Wantai Biological Pharmaceutical Co., LTD, Beijing, China) following the manufacturer's instructions. The results were scored using a microplate reader with a single filter (450 nm) or a dual filter (450 nm, 600-650 nm). According to the manufacturer's instructions, the TB-IGRA assay result was deemed positive if the value of T-N was ≥14 and N/14 (T: concentration of testing tube; N: concentration of background control tube) (12, 13).

Definitions and statistical analysis 2.7.1. Index case
The index case is an initially identified case of new or recurrent TB in a person of any age in a specific household or another comparable setting in which others may have been exposed (14).

Cluster epidemic situation
For a highly concentrated unit, such as schools, two or more cases of TB with epidemiological correlation, which occurred within 3 months, resulted in a TB cluster epidemic.

Secondary confirmed cases
Secondary confirmed cases met the Pulmonary TB Diagnosis Criteria WS288-2017 and were epidemiologically related to the index case during the cluster epidemic period (May to December 2021).

Standard of the criterion for the TST result
Tuberculin skin test method: Briefly, 5 IU tuberculin purified protein derivative (PPD) was injected into the anterior one-third center of the palmar side of the left forearm, and skin induration was examined after 72 h according to the Technical Guidelines for tuberculosis Prevention and Control in China 2021 (15). The TST result is interpreted as follows: (1) Negative (−), the mean induration diameter is less than 5 mm, or no reaction was observed; (2) Generally positive (+), the mean induration diameter is ≥5 mm but <10 mm; (3) Moderately positive (++), the mean induration diameter is ≥10 but <15 mm; (4) Strongly positive (+++), the mean induration diameter is ≥15 mm or the skin at the injection site has local double circles, bullae, necrosis, and lymphangitis. The judgment principle for LITBI: no BCG vaccination and interference from non-tuberculosis mycobacteria (NTM), TST ≥ 5 mm; And BCG vaccinators and/or NTM endemic areas, TST ≥ 10 mm. Definition of positive TST conversion: If the previous TST result was less than 5 mm, the repeat test result should be ≥10 mm; if the previous test had a TST result of 5-10 mm, the repeat test result should increase by 10 mm or more.

Criteria for close contacts
The criteria for judgment and screening of close contacts are based on "the expert consensus on epidemiological investigation and on-site management of school tuberculosis outbreaks" (16). Index cases exhibiting positive etiology, pulmonary cavity, or symptoms of pulmonary TB should adhere to the following criteria: personnel who were in direct contact with the index case for 8 h or more, or whose cumulative contact time reached or exceeded 40 h, in the same class, dormitory, or other enclosed spaces within the 3 months preceding diagnosis until 14 days post-treatment. Conversely, an index case with negative etiology, no cavity, and no symptoms should meet the Frontiers in Public Health 05 frontiersin.org following criteria: personnel in the same class and dormitory as the index case from 1 month before diagnosis until 14 days posttreatment. In the event that new TB cases are identified among close contacts, the screening parameters of contacts will be expanded according to the level of exposure. Individuals working in the same office building and/or residing on the same dormitory floor would be considered general contacts.

Statistical analysis
Data in this study were analyzed using GraphPad Prism software 9.5.1 version (San Diego, CA, United States). Fisher's exact test was used for categorical variables with small sample sizes, and the chi-square test was used for categorical variables with large sample sizes. A p-value of less than 0.05 was considered a significant difference. Furthermore, the calculations of the relative risks (RR) with a 95% confidence interval (CI) were done by Poisson regression. Exact Poisson regression was used when a cero frequency was found. The program Stata ® (Stata Corp., College Station, Texas, United States) was used for this analysis (17).

Information of the index case
The index case was a 23-year-old male patient that developed chest pain and intermittent fever in May 2021 without prominent cough, expectoration, or other symptoms. Chest CT revealed patches and nodular hyperdense shadows with cavity formation in the upper lobe of the left lung ( Figure 2). The following results were obtained from the examination of the blood sample: (1)  3.2. The results of symptoms, TST, IGRA, and chest CT in close contacts screened in the first round of screening The index case was a patient with a positive TB etiology and lung cavities. According to the criteria for a close-contact population, 32 screening objects were primarily identified. The detailed information of these 32 close contacts can be found in Table 1. Furthermore, we also determined the symptom, chest CT, TST, and IGRA of these close contacts. Our results showed that the positivity rate of symptom screening was 0.00% (0/32), the positivity rate of TST (+, ++, and +++) was 65.60% (21/32), the strong positivity rate of TST (+++) was 34.40% (11/32), and the latent infection rate of TST (++ and +++) screening was 43.75% (14/32) ( Table 2). Furthermore, we also found that the positivity rate of IGRA and chest CT in 32 close contacts were 9.30% (3/32) and 0.00% (0/32), respectively ( Table 2). Based on these screening results, three close contacts with a strong positivity rate of TST (+++) and a positivity rate of IGRA received prophylactic medication, while the remaining close contacts were followed up for 3 months.

Comparison of TST, IGRA, and chest CT results of general contacts and close contacts screened in the second round of screening
We further compared the results of TST, IGRA and chest CT between general contacts and close contacts screened in the second round of screening. Our results showed that (Table 4)    Yes, the current evidence supports preventive anti-tuberculosis treatment for close contact and the close contact agreed and received preventive therapy; Refuse, the current evidence supports preventive anti-tuberculosis treatment for close contacts but the close contact did not consent to prophylactic treatment; No, the current evidence does not support preventive anti-tuberculosis treatment for close contact and the close contacts did not receive preventive therapy.
Frontiers in Public Health 07 frontiersin.org TB infection (diameter ≥ 10 mm) in close contacts of the index case was 2.5 times higher than that in general contacts (p = 0.037, RR 2.50, 95%CI: 1.06-5.91). In addition, we found that the risk of IGRA positivity or abnormal chest CT in close contacts of the index patient was not significantly different from that in general contacts (Table 4).

Secondary cases
Five secondary cases (case 2, case 3, case 4, case 5, and case 6) were found in the second round of screening ( Figure 3). By comparing the first and second screening results of the 5 secondary cases, we found that the TST results of all 5 secondary cases changed from negative or weakly positive to strongly positive, the IGRA results suggested that case 4, case 5, and case 6 changed from negative to positive, and the chest CT indicated that all 5 secondary cases had lung injury (Table 5). In addition, the pathogenic diagnostic results revealed positive sputum LAMP result in case 3, positive sputum culture and Xpert results in case 4, and positive BALF LAMP result in case 5 (Table 5). Furthermore, we also conducted an epidemiological investigation. During the 3-month symptom monitoring, there were no obvious symptoms such as cough, expectoration, and fever in these Comparison of the first and second screenings of chest CT for the five secondary cases. In the first screening, no abnormalities were found in the chest CT for the 5 cases. Three months later, the second screening of chest CT was performed, and new lesions were observed in the lungs of all five cases. Left: the first CT image for each case; Right: the second CT image for each case.
Frontiers in Public Health 08 frontiersin.org 5 secondary cases. Only case 4 experienced chest pain and fever at the 11th week. Of these 5 secondary cases, 3 were in the same dormitory with index case, while the other two cases from different dormitories were also good friends of index case and often stayed together ( Figure 4). These results suggest that the risk of exposure was highest among persons living in the same room with index case, but that the LTBI population may have some resistance to transmission from the index case. In addition, we found that even people who did not live in the same room were susceptible to infection if they had frequent, unprotected interactions with index case.

Analysis of TB risk factors of secondary cases
To further determine the potential risk factors for active TB in close contacts of the index case, we analyzed the factors (age, BCG scar, residence area, and exposure levels) in 32 close contacts with or without LTBI and active TB. Our results showed that among these four factors, the exposure level was the significantly higher risk factor for the occurrence of secondary cases (p = 0.030, RR = 13.5, 95% CI: 1.21-141.77, Table 6).

Lessons and recommendations
According to the handling of the above TB epidemic situation, the scope of close contacts and the appropriate handling process should be determined according to whether the indicated case is infectious. The specific process is shown in Figure 5.

Discussion
We reported an outbreak of tuberculosis in Beijing, China. Since the index case sought medical treatment due to symptoms in May 2021, an epidemiological investigation was quickly carried out. The index case was a university student with cavitary pulmonary TB diagnosed by the positive sputum bacteria and strong infectivity. To minimize transmission from the index patient to close contacts, we conducted a thorough search of all contacts of the index patient from 3 months before diagnosis to 14 days after treatment initiation and identified 32 close contacts. In this study, the close contacts should be individuals shared the same class or dormitory with the index case, have been direct contacted with the index case in other confined spaces for more than 8 h, or have been in contact with the index case more than 40 h. TST, chest CT, and IGRA were performed for the close contacts. The latent infection rate of the TST screening (above moderately positive) was 43.75% (14/32) and the IGRA positivity rate was 9.30% (3/32). No abnormalities were found via chest CT screening. According to the technical guidelines for tuberculosis prevention and control, TST positive and IGRA positive contacts gave isoniazid (0.3 g once daily) and rifampicin (0.6 g once daily) preventive treatment for 3 months (15). The second round of screening was performed 3 months after symptom monitoring. In some countries, the second screening is made 8-10 weeks after the first (18). Five secondary cases were found, all of which were previously IGRA negative and/ or TST negative (two cases with TST 5 mm × 5 mm had a history of BCG injection in childhood) and were TST strongly positive and IGRA positive in the second round of screening. Accordingly, close contacts were identified to be in the window period for TB infection at the time of the first screening. Thus,  Frontiers in Public Health 09 frontiersin.org the window period for TB infection requires more vigilance to manage TB epidemic in the army, school, or other collective living groups. In addition, the population without LTBI in the initial screening stage should receive more attention than the individuals with LTBI, as their risk of TB is more significant.
In the fourth national epidemiological survey conducted in China in 2000, the estimated natural infection rate of TB in the entire age group was 44.50% based on TST. In 2013, Gao et al. performed a population-based, multicenter, prospective study on LTBI. Based on their findings, TST might overestimate the LTBI rate in the Chinese population compared with IGRA. The TST positivity rate (TST ≥ 10 mm) ranged from 15.50 to 41.70% after age and sex standardization, and the IGRA positivity rate ranged from 13.50 to 19.80% (19). The IGRA positivity rate of the close-contact population of patients with TB was relatively higher (32.00-48.00%) (13,20). Wu et al. (21) reported that the TST and IGRA positivity rates of newly recruited soldiers in the Chinese army were 41.00 and 21.00%, respectively. When the latent M. tuberculosis infection rate of close contacts screened in this study was lower than or equal to that of the general population, the screening period of some TST-negative close contacts may have coincided with the window period of LTBI. Therefore, interventions comprising 3 months of symptom  Schematic representation of the living environment of five secondary cases and index cases. In the first round of screening, we identified two individuals with LTBI who shared a room with the index patient, one of whom received prophylaxis because of a strongly positive PPD and IGRA(+). In the second round of screening, we identified 5 close contacts who developed ATB (secondary cases), including case 2, case 3, case 4, case 5, and case 6. Interestingly, case 3, case 4, and case 5 lived in the same dormitory (Room B) with the index case, and case 2 (Room A) and case 6 (Room C) were close friends who did not live in the same dormitory with the index case.
Frontiers in Public Health 10 frontiersin.org monitoring, 3 months of re-examination after the initial screening, and preventive treatment only for close contacts with strong TST positivity would ignore some hidden secondary cases and affect the prevention and control effectiveness of the epidemic. Currently, the scope of such screenings is limited. As a result, when the close contacts were screened for collective living groups with etiologically positive and highly infective indicator cases, and their screening period was in the window period, it was unclear whether the scope of preventive medication should be reconsidered according to the degree of their close contact with the index cases. The five patients newly diagnosed after our second screening were all Level 1 close contacts (roommates, close friends) of the index case. Hence, as the screening period was in the window period of tuberculous infection, preventive medication should be administered according to the degree of close contact, regardless of the basis for tuberculous infection, at least for the same dormitory personnel. Three months after the first screening, the second screening found five cases of TB with pulmonary lesions, one with pleural effusion. During this period, we conducted symptom monitoring, there were no obvious symptoms such as cough, sputum and fever in these 5 secondary cases. Only case 4 had chest pain and fever in week 11. In some countries, the second screening is performed 8-10 weeks after the first (18). Generally, the close contacts would receive chest CT once every 3 months to explore new cases, considering the window period of LTBI and it would have high risk of TB infection during this time, 3 months was too long to control TB transmission of new cases. Therefore, the close contacts should be subjected to chest x-ray or CT once a month to detect new cases, because chest x-ray would not show some initial and small lesions in the lung, leading to missed diagnosis, and CT may cause high-dose radiation exposure. Low-dose spiral CT would be recommended for monthly re-screening to detect and treat the disease earlier, effectively block the epidemic and control the spread of the disease.
Bacille Calmette-Guerin (BCG) is the only approved vaccine used to prevent TB, its target population includes infants and newborns. However, the preventive effect of BCG vaccine against TB is limited. Continuous stimulation can only induce short-lived effector memory T cells and effector T cells (22). Therefore, the immune effect of BCG weakens with age increasing. In 1995, the World Health Organization highlighted the lack of a scientific basis for BCG revaccination, and the Chinese Health Commission was discontinued in 1997. Nevertheless, some countries still selectively vaccinate high-risk populations, including close contacts with TB. Further, the immune effect of revaccination has been proven to be effective. BCG vaccines can induce immunity training to enable nonspecific immune protection, and this protective effect is continuous (23, 24). According to some studies, revaccination with BCG could reduce the likelihood of TB transmission, with the risk of infection reduced from 5.70% per year to 4.80% per year; infection could be avoided in 17.00% of cases (25). We have analyzed the relationship between a history of BCG vaccination and LTBI and found that there was no significant relationship between a history of BCG vaccination and latent infection, as the protective effect of BCG generally lasts for 5-10 years. Although previous studies have shown that young women living in rural areas, those living in western China, and those without a history of BCG vaccination are more likely to develop severe TB (26), the protective effect of BCG in adults is poor (27,28). Hence, all collective living groups (students, workers, older adults living in nursing homes, and prisoners) should receive TB screening, and personnel without a latent infection should be revaccinated with BCG. Such intervention may reduce the likelihood of a TB outbreak.
During the management of this epidemic, we expanded the scope to screen 28 general contacts after new cases were identified during the second screening. Among the 28 patients, the positivity rate of TST was 28.60% (8/28), strong positivity rate of TST was 14.30% (4/28), and latent infection rate of TST screening (above Frontiers in Public Health 11 frontiersin.org moderate positivity) was 25.00% (7/28). The IGRA positivity rate was 10.70% (3/28). The positivity rate of CT screening was 0.00% (0/28). The TST positivity rate (78.00%), latent infection rate (62.50%), and strong positivity rate (50.00%) of close contacts were significantly higher than those of general contacts (p < 0.05). The positivity rate of IGRA in close contacts was 25.00% and higher than that in general contacts (10.70%), however, the difference was not statistically significant, which might be related to the small sample size. IGRA is less sensitive than TST in terms of its screening effects on the latent infection of tuberculous bacteria; however, its specificity is high (29). The detection of TST is affected by many factors, such as BCG vaccination, environmental non-TB mycobacteria (NTM), and host immune status. The specificity of the results was poor and false positives were detected (30). Although IGRA was not affected Epidemiologic investigation of the tuberculosis outbreak and the process of screening, diagnosis, prevention and treatment of index cases and close contacts.
Frontiers in Public Health 12 frontiersin.org by BCG and most NTM (12,31), its sensitivity was low. The five secondary cases of active pulmonary TB showed a strongly positive reaction based on TST detection, and two remains IGRA negative, which is inconsistent with the results of previous studies. We considered that IGRA was detected using an in vitro experiment, which might have different degrees of false-negative reactions owing to the influence of many factors, such as blood sample collection, blood sample processing, experimental platforms, artificial technical operations, and reagents. Therefore, the combined application of the TST and IGRA in the screening of a close-contact population of TB can improve the sensitivity and specificity of screening for latent TB infection and can achieve complementary advantages to a certain extent. This study also has some limitations: (1) How the index case became infected with M. tuberculosis was not clear; (2) Although this study analyzed the risk of age, BCG scarring, area of residence, and exposure level for close contacts of reported cases, it did not evaluate the role of risk factors such as body mass index, smoking, alcohol consumption, use of other drugs, and disease in the development of secondary cases in close contacts; (3) The differences in plasma cytokine levels and the absolute number and percentage of lymphocyte subsets in the peripheral blood of close contacts in the first and second screening rounds were not analyzed, thus missing the opportunity to further analyze the etiology of secondary cases; (4) The conclusions and recommendations of this study are based on a study with a small sample size, and studies with larger sample sizes are needed to verify our conclusions.

Conclusion
In summary, we addressed a TB cluster outbreak and conducted two rounds of screening for the close contacts of a diagnosed TB case. The results evidently differed from those of previous studies. After the second screening, five secondary cases of pulmonary TB were found to have a negative result (TST/IGRA/chest CT) in the first screening. Thus, the window period of LTBI in close-contact screening should be prioritized, and more attention should be paid to people without any LTBI in the preliminary screening, whose risk of pulmonary TB would be more significant owing to a lack of TB antibody. Accordingly, it is worth considering whether the interval between the two screenings should be shortened and whether the indication of preventive medication should be changed. Thus, in addition to individuals who are strongly positive, other individuals should also receive preventive medication according to their degree of exposure.

Data availability statement
The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding authors.