Development of in vitro methods to model the impact of vaginal lactobacilli on Staphylococcus aureus biofilm formation on menstrual cups as well as validation of recommended cleaning directions

Introduction Menstrual cups (MC) are a reusable feminine hygiene product. A recent publication suggested that Staphylococcus aureus (S. aureus) biofilms can form on MCs which may lead to increased risk of menstrual Toxic Shock Syndrome (mTSS). Additionally, there is concern that buildup of residual menses may contribute to microbial growth and biofilm formation further increasing mTSS risk. Quantitative and qualitative analysis of in vitro tests were utilized to determine if S. aureus biofilm could form on MC in the presence of the keystone species Lactobacillus after 12 h of incubation. The methodology was based on a modification of an anaerobic in vitro method that harnesses the keystone species hypothesis by including a representative of vaginal lactic acid bacteria. Methods MCs were incubated anaerobically for 12 h in Vaginal Defined Media (VDM) with the two morphologically distinct bacteria, Lactobacillus gasseri (L. gasseri) and S. aureus. Colony Forming Units (CFU) for each organism from the VDM broth and sonicated MC were estimated. In addition, a separate experiment was conducted where S. aureus was grown for 12 h in the absence of L. gasseri. Qualitative analysis for biofilm formation utilized micro-CT (µ-CT) and cryogenic scanning electron microscopy (Cryo-SEM). Results Samples collected from the media control had expected growth of both organisms after 12 h of incubation. Samples collected from VDM broth were similar to media control at the end of the 12-h study. Total S. aureus cell density on MC following sonication/rinsing was minimal. Results when using a monoculture of S. aureus demonstrated that there was a significant growth of the organism in the media control and broth as well as the sonicated cups indicating that the presence of L. gasseri was important for controlling growth and adherence of S. aureus. Few rod-shaped bacteria (L. gasseri) and cocci (S. aureus) could be identified on the MCs when grown in a dual species culture inoculum and no biofilm was noted via µ-CT and cryo-SEM. Additionally, efforts to model and understand the validity of the current labeled recommendations for MC cleaning in-between uses are supported. Discussion The data support continued safe use of the Tampax® cup when used and maintained as recommended.

Introduction: Menstrual cups (MC) are a reusable feminine hygiene product.A recent publication suggested that Staphylococcus aureus (S. aureus) biofilms can form on MCs which may lead to increased risk of menstrual Toxic Shock Syndrome (mTSS).Additionally, there is concern that buildup of residual menses may contribute to microbial growth and biofilm formation further increasing mTSS risk.Quantitative and qualitative analysis of in vitro tests were utilized to determine if S. aureus biofilm could form on MC in the presence of the keystone species Lactobacillus after 12 h of incubation.The methodology was based on a modification of an anaerobic in vitro method that harnesses the keystone species hypothesis by including a representative of vaginal lactic acid bacteria.Methods: MCs were incubated anaerobically for 12 h in Vaginal Defined Media (VDM) with the two morphologically distinct bacteria, Lactobacillus gasseri (L.gasseri) and S. aureus.Colony Forming Units (CFU) for each organism from the VDM broth and sonicated MC were estimated.In addition, a separate experiment was conducted where S. aureus was grown for 12 h in the absence of L. gasseri.Qualitative analysis for biofilm formation utilized micro-CT (µ-CT) and cryogenic scanning electron microscopy (Cryo-SEM).Results: Samples collected from the media control had expected growth of both organisms after 12 h of incubation.Samples collected from VDM broth were similar to media control at the end of the 12-h study.Total S. aureus cell density on MC following sonication/rinsing was minimal.Results when using a monoculture of S. aureus demonstrated that there was a significant growth of the organism in the media control and broth as well as the sonicated cups indicating that the presence of L. gasseri was important for controlling growth and adherence of S. aureus.Few rod-shaped bacteria (L.gasseri) and cocci (S. aureus) could be identified on the MCs when grown in a dual species culture inoculum and no biofilm was noted via µ-CT and cryo-SEM.Additionally, efforts to model and understand the validity of the current labeled recommendations for MC cleaning in-between uses are supported.

Introduction
Staphylococcus aureus is a highly versatile and adaptable grampositive pathogen.It can inhabit the skin and mucous membranes as a harmless commensal (1) and is most often found as a commensal vaginal organism in most reproductive-aged women (2,3).With the insertion of tst in its genome, S. aureus can produce the superantigen, TSST-1.TSST-1 is associated with the pathogenesis of menstrual Toxic Shock Syndrome (mTSS); a rare, recognizable, and treatable disease that can occur in women who have low (<1:32) titers of neutralizing antibody to TSST-1 (4) during or around the time of menstruation (5).Although severe mTSS cases have occurred in women who have never used an intravaginal device, epidemiological research has linked intravaginal device use as a risk factor for mTSS (5).As a facultative anaerobe, S. aureus generates energy through aerobic respiration when present in an oxygen-rich environment but can switch to anaerobic respiration and fermentation for survival when present in oxygen deficient environments.While S. aureus can grow anaerobically, TSST-1 production requires oxidative metabolism.Certain strains of S. aureus can produce the superantigen, TSST-1 under the appropriate environmental conditions such as 21% O 2 and 5% CO 2 (6).Other environmental factors such as a neutral pH, are required for in vitro TSST-1 production (7,8).
Several physiological mechanisms maintain the health of the vagina including the composition (richness and diversity) of the vaginal microbiome.Maintenance of high numbers of vaginal lactic acid bacteria have been identified as the hallmark of health.Since 1896, emphasis has been on members of the genus Lactobacillus as a keystone species due to their ability to ferment sugars to produce lactic acid (9)(10)(11).Lactic acid bacteria that predominate in the vagina of reproductive-age women (12) metabolize extracellular glycogen stored in epithelial cells into lactic acid by anaerobic glycolysis and fermentation-thereby lowering the vaginal pH to create an inhospitable environment for many pathogenic bacterial and viral species (13).Importantly, lactic acid bacteria are thought to serve as the keystone species in maintaining the health of the vagina (14-16) and have been shown to prevent the overgrowth of opportunistic pathogens such as S. aureus (14,17) which are also found in vagina of some women (2).Lactic acid bacteria, found along the vaginal walls and cervix in reproductive age women (18) have been shown to suppress TSST-1 production in some populations of women; additional work is needed to define the characteristics responsible for this suppression of .Different species of lactobacilli can produce a variety of compounds such as lactic acid (L.gasseri) (20), hydrogen peroxide (e.g., L acidophilus, L. crispatus, L. gasseri) (21) and even tetramic acids (L.reuteri) (22) which have been shown to reduce IL-8 in vaginal epithelial cells (23) and even inhibit quorum sensing activities of S. aureus (24).
In nature, microorganisms, including S. aureus, exist primarily by attaching to and growing upon abiotic and biotic surfaces (25,26).These surface-attached microbial agglomerations were named "biofilms" for the first time in 1978 (27).Contributing to the potential pathogenicity of S. aureus is its ability to form biofilms where single cells are aggregated and embedded in a protective matrix that may or may not be adhered to a surface (25).Once in this aggregate, cell-signaling may occur through quorum sensing via the agr system (28).Agr has various biological functions: (1) regulating the expression of staphylococcal virulence factors and (2) facilitating the structuring and detachment of bacteria biofilms.These functions are crucial for the pathogenesis of staphylococci and are always associated with the pathogenicity of highly virulent S. aureus (29).This system controls a variety of virulence mechanisms including production of TSST-1.
Biofilms have been identified on human tissue surfaces, including human vaginal epithelial cells (30-32).However, these adherent bacteria do not typically mature into thick biofilms given that there is a complete turnover rate of 96 h by the vaginal tissues (33).Adherent bacteria and biofilms that originate from the vaginal microbiota have been identified on a variety of removable vaginal medical devices such as tampons, pessaries, and menstrual cups [MC(s)] (31, 32) with the biofilm thickness and diversity related to the labeled wear time (34).In these cases, active disease is absent in a high percentage of women (31,32).Because TSST-1 positive strains can produce higher concentrations of TSST-1 when cultured in biofilm compared to individual, adherent bacteria (6), it is an important element of the overall safety program to understand the potential for biofilm to form on intravaginal durable medical devices.
A MC is a type of reusable feminine hygiene product that is a small, flexible funnel-shaped device that many women use as an eco-friendly alternative to tampons and has been commercially available in the US for managing menstrual flow since 1937 (35).The MC is folded and inserted into the vagina below the cervix, where it collects rather than absorbs menstrual flow.Depending on the manufacturer and the size of the cup, MCs can hold 10-38 ml of menses.MCs can be worn up to 12 h, depending on a women's menstrual flow, then removed and menses disposed.As durable products, MCs are cleaned in between use instead of being disposed after a single use (36).MCs are often made of medical-grade silicone, while a few brands use thermoplastic elastomer (TPA) or rubber (37).
The Tampax MC, which is the focus of this research, is comprised of a single material, liquid silicone rubber, has a recommended use period of one year.Recommended cleaning instructions (as indicated on the product insert) include washing the cup with soap and water between uses throughout the menstrual cycle and boiling the cup for five to seven minutes prior to the initial use and at the end of each menstrual cycle, prior to storing the cup (38).

Research questions
A recent publication by Nonfoux et al. (39) using in vitro testing methods stated that S. aureus biofilm formation on menstrual cups after 8 h can lead to bacterial colonization on the product that potentially alters mTSS risk and the safe use of MCs.The same publication suggested that current cleaning protocols are not sufficient to remove biofilm (39).Subsequently, a publication by Wunsch et al. (40), investigated various cleaning techniques noting that cleaning with cold tap water (with or without soap) followed by steeping in boiled water for 5 min was most effective at reducing viable bacteria.Thus, the objectives of this research report centered on whether S. aureus biofilms would form on the MC in the presence of lactic acid bacteria (keystone species) and if the instructions on the current package of the Tampax MC were sufficient manage the cleaning (both effectively and easily) of the MC in between uses.The specific research questions included: 1. What are the best environmental conditions to use in an in vitro test system to mimic the exposure of the menstrual cup to the vaginal environment and microbiota? 2. Does the presence of L. gasseri, a vaginal lactic acid bacteria (acting as keystone species) alter the likelihood of S. aureus biofilm formation quantitatively or qualitatively on the MC when the two organisms (dual species system) are incubated for 12 h (labeled wear time of the Tampax MC) in cell densities found in the human vagina?3. Are the current cleaning instructions for the Tampax cup sufficient to remove adherent bacteria based on the results of in vitro test analyses?

Test articles (MC)
The Tampax ® MC (The Procter & Gamble Company, Cincinnati, OH) is composed of Class VI medical grade silicone which meets the highest Class VI medical grade classification following the U.S. Pharmacopeia criteria for the classification of plastics (41).Three different product iterations of the Tampax MC were used in this research: The currently marketed Tampax menstrual cup is comprised of QP1-40 Class VI medical grade silicone as supplied by Dow/Dupont.The QP1-20 menstrual cup is not marketed but is identical to the QP1-40 menstrual cup except for minor differences in starting materials.Two different prototypes of the QP1-20 cup were tested; the only difference between these two MCs (noted as Prototype 1 and Protype 2) is the initiation of a 4 h curing step which was utilized for Prototype 1 only.Tampax MCs come in "regular" and "heavy" flow sizes; all testing was conducted on the "heavy flow" size of the Tampax MC as it is the cup with greatest surface area, and therefore, regarded as the worst case scenario for biofilm formation.The MCs used in both clinical and in vitro experiments as outlined in this paper are also summarized in Supplementary Table S-1.

Bacterial strains
All strains were selected from the ATCC strain collection (https://www.atcc.org/)and selected because they were sourced from the human vagina.S. aureus subsp aureus Rosenbach ATCC 33589 D1470 (587), a facultative anaerobe isolated from vagina of TSS patient and L. gasseri ATCC 9857, an aerotolerant anaerobe isolated from the vagina, were used throughout the quantitative and qualitative biofilm formation assays.S. aureus Rosenbach ATCC 33589, and Escherichia coli ATCC 8739, were used in the MC cleaning study.S. aureus, a Gram-positive organism, was chosen because it was the relevant organism for this study.E. coli, a Gram-negative enteric bacterium, was chosen as a second organism because it is traditionally used in cleaning, disinfection, and sterilization studies to measure log reduction and sufficiency of the applied cleaning methodology (42)(43)(44).

Media
Chemically Defined Medium (CDM), originally described by Geshnizgani (45) and referred herein as Vaginal Defined Media (VDM) is a complex formula, compositionally similar to that of the female genital tract of reproductive women and contains hemin and mucins that provide a nutrient rich environment that mimics the in vivo ecosystem.This formula is sufficient to support long term growth of microorganisms in safety studies that span a duration greater than several hours and is most appropriate for quantifying bacteria growth and/or biofilm formation in the presence of vaginal medical devices as indicated by several published studies (38,(46)(47)(48).Formula refinements were reported by Elkins et al. (49), for use in a dual-species in vitro system and it is this medium, formulated at a pH of 6.5 ± 0.2 to simulate the near neutral pH of the vagina environment during menstruation that was used in the quantitative and qualitative biofilm studies.
Artificial Menstrual Fluid (AMF) is a simple and thicker suspension media commonly used to investigate fluid handling properties of absorbent menstrual products.AMF was selected for the cleaning experiments due to its viscosity which allowed better adherence of the artificial microbial soil to the cup.

Experimental procedures 3.4.1. Research question #1: determination of appropriate in vitro environmental conditions
Two independent experiments were conducted to determine the best environmental conditions, aerobic or anaerobic, to use in an in vitro test system to mimic the exposure of the menstrual cup to the vaginal environment and microbiota.The first experiment aimed at understanding if the menstrual cup could introduce oxygen during MC insertion potentially altering the normally anaerobic nature of the vagina; the second experiment aimed at understanding if the position of the menstrual cup would alter the physical opening of the vagina allowing entrance of oxygen during MC wear.

Understanding potential to introduce oxygen with Tampax menstrual cup insertion
The following procedure was used to determine possible residual oxygen remaining in the Tampax cup after folding and insertion into the vagina and then correlating the measurement with oxygen content based on ideal gas law calculations.Two folding techniques described in the package insert of the Tampax cup are: the C fold and punchdown fold (Figure 1).A heavy flow Tampax menstrual cup was held upright (cup open end pointed upward and level to avoid spilling) and filled to the rim using Millipore water.The cup was folded either using the C fold or punchdown fold technique causing some of the water to be expelled leaving water only in the void volume (volume remaining after folding).Water remaining in the cup was measured on an analytical balance.The ideal gas law, (PV ¼ nRT) was used to calculate the amount of oxygen that would be present in the cup after folding where: P = 1 atm.V = void volume in cup (in liters) n = moles of air R = gas constant (0.08206 L•atm/K•mol) T = 310.15K (body temperature in Kelvin).
The equation was solved for n (number of moles of air) and multiplied by the concentration of oxygen in the atmosphere (20.95%).
Subsequently, the time to reach low or zero O 2 was calculated using the starting (131.00 torr) and ending (98.00 torr) partial pressure from Wagner et al. (50) and the following assumptions to calculate the rate of change of O 2 : pressure remains constant [total pressure at elevated body temperature calculated using Gay Lussac's Law P 1 /T 1 and P 2 /T 2 where P 1 is pressure of air at ambient temperature (1 atmosphere), T 1 = 273.15K, P 2 is unknown and T 2 = 310.15K (body temperature); solving for the unknown (P 2 = the pressure of the void volume at body temperature) yields 1.13 atm or 862.95 torr at 37°C], the decrease in partial pressure of O 2 increases partial pressure of all other gasses in air, an increase in partial pressure of N 2 and other gasses in air is proportional to volume decreases via and the volume decrease is due to absorption of O 2 only.
As Robb (51) noted, oxygen can diffuse through silicone rubber, 0.014 cm 3 /min, but the diffusion rate per cm 2 is quite slow relative to the rate of absorption by the body (50).A silicone rubber Tampax MC ranges in thickness from approximately 3 to 5 mm, a fairly thick barrier to O 2 on the timescale of this study.
The mechanism of O 2 decline is suspected to be by absorption through capillaries and subsequently into the tissues/cells that are supplied by local capillaries (52, 53).Finally, movement of the diffused oxygen will enter epithelial cells which is crucial for mitochondrial respiration (54, 55) Also, bodily fluids, such as menses, which flow from the uterus into the vaginal vault via the cervix are moving and in flux (56).It is postulated that the small amount of O 2 in the cup has dissipated into the surrounding tissues within 30 min.

Clinical study to determine anatomical location of menstrual cup after insertion
An observational study was designed (Sponsor: The Procter & Gamble Company), and the protocol and informed consent documents sent to Advarra IRB (Columbia, MD, USA) for review.The study was conducted in compliance with the applicable Federal Regulations Guidelines for Good Clinical Practice (57).Inclusion and exclusion criteria were reviewed with each potential subject and if all inclusion and none of the exclusion criteria were met, the subject was accepted into the study after completing informed consent.Inclusion and exclusion criteria are contained in Supplementary Table S-2.Effort was made to enroll a group of women with a wide range of Body Mass Indices (BMIs) representative of the user population; BMI of the premenopausal women was recorded.Subjects had regular periods, had at least 1 child and were comfortable using intravaginal devices.MRI scans were obtained at Proscan Tri-County, OH.No imaging aids were added to the MC except an imaging marker was placed at the vaginal opening.Images were taken: scout (obtained for localization purposes allowing the technician to select the area of dedicated image acquisition), supine, axial, sagittal, coronal, and sagittal (during valsalva maneuvers).Images were analyzed using RadiAnt DICOM viewer.

Research question #2: does the presence of L. gasseri alter the likelihood of S. aureus biofilm formation quantitatively or qualitatively?
In this study, the determination of biofilm was based on characterization of biofilm viability using quantitative indirect methods (cfu/ml determination following sonication) complemented by direct qualitative measure to visualize the morphology (μ-CT and cryo-SEM).

Quantitative evaluation of biofilm formation
The quantitative biofilm formation study was performed multiple times.Each experiment included one beaker designated as the media control (Vaginal Defined Media, VDM, and a dual species culture of S. aureus and L. gasseri) and at least 3 beakers designated as broth (VDM, a co-culture of S. aureus and L. gasseri and the Tampax cup).In experiments which encompassed a qualitative assessment of biofilm formation, an additional beaker was included so that the total number of beakers for quantitative assessment remained at 3 (Figure 2).
Each cup was submerged and suspended upside down in the vertical position inside a sterile beaker containing 250 mL of VDM with a dual species culture of 10 4 cfu/mL S. aureus (ATCC 33589) and 10 8 cfu/mL L. gasseri (ATCC 9857).The original S. aureus cell density was selected based on human vaginal washing samples from Johnson, et al. (58), and L. gasseri cell density was selected based on analysis of microbial composition of human vaginal swabs (59).Based on the results of earlier experiments (section 3.4.1.1 and 3.4.1.2) supporting anaerobic conditions for the in vitro study, the beakers were incubated in an anaerobic chamber (Anaerobe Chamber Coy Model Type B Serial number AC12020) at 35°C ± 2°C without shaking for 12 h.Following this 12 h period, samples were collected for plating and enumeration from the dual species culture media control and broth using selective media under appropriate growth conditions: Mannitol Salt Agar (MSA) for S. aureus (aerobic) and Human Bilayer Tween (HBT) (Remel R0147/VWR 90008-708) for L. gasseri (anaerobic).Plates were incubated for 48 h ± 2 h at 35 ± 2°C and cfu/mL were determined separately for both S. aureus and L. gasseri.The maximum incubation time of 12 h was selected since the labeled wear time for the Tampax MC is up to 12 h.
Additionally, the MC was removed from the broth and gently rinsed 3 times with 300 mL phosphate-buffered saline (PBS) (Difco), before sonicating in 200 ml of PBS for 1 min using the BactoSonic instrument (Bandelin Electronics).Sonication was utilized to release any adhered organisms from the menstrual cup, samples were taken from the PBS for plating and enumeration and cell density counts for both S. aureus and L. gasseri were determined as described previously for the media control and broth samples.The simple ultrasound sonication protocol applied prior to cfu determination has been demonstrated to disrupt large planktonic aggregates of S. aureus otherwise recalcitrant to disruption by shear forces to obtain accurate estimates of cell numbers in laboratory liquid cultures of S. aureus (60).
From these data points, the following comparisons were made: • Cell density values (cfu/mL) obtained from three cup types made of medical grade silicone were compared to understand if there were significant differences in organism recovery between the cups at 12 h.• The number of organisms recovered from the media control (without MC) was compared to the number of organisms recovered from the broth (with MC) to evaluate the impact of the cup on organism growth.• The number of non-adherent (planktonic) organisms recovered from the broth was compared to the organisms recovered from the sonicated cup to quantify the number of organisms adhered to the cup.
In all comparisons, cell density data were log transformed prior to statistical analysis.Additionally, each data point recovered from the sonicated cups was normalized against the broth volume before used for statistical analysis to allow for appropriate comparison (200 mL PBS vs. 250 mL media).Across all experiments, the total number of datapoints for Media Control and Broth was n = 5 and n = 16, respectively.Each of these 16 Broth beakers contained a single menstrual cup.Two of these cups, randomly selected, were removed from the Broth, and sent without further manipulation (e.g., no rinsing/ sonication) for qualitative assessment of S. aureus and L. gasseri via µ-CT and cryo-SEM (Section 3.4.2.2.1).Cell density values were not determined from the cups sent for qualitative analysis.The remaining 14 cups were processed for quantitative biofilm evaluation after sonication.Fourteen successful data points were generated for L. gasseri, and eleven successful data points were generated for S. aureus.Three S. aureus data sets were excluded due to being outside of countable range.
An additional experiment was conducted with S. aureus alone in monoculture (n = 3) using the same reagents and preparation; the only difference being that L. gasseri was not added to the test system.In this study, both quantitative and qualitative assessment was conducted; one MC, randomly selected, was submitted for µCT and cryo-SEM analysis to identify any possible biofilm formation.

Qualitative evaluation of biofilm formation
Qualitative analyses via μ-CT and cryo-SEM were conducted for MCs that were incubated in the presence of the microbial dual species culture of L. gasseri and S. aureus as well as monoculture of S. aureus.Consistent with the published literature (31), biofilm was defined as ≥ 7 bacterial cells clustered to form a surfaceattached structure.
3.4.2.2.1.Contrast-enhanced micro-computed tomography: biofilm tracking of menstrual cup.MCs were incubated with the dual species of cultured bacteria (L.gasseri and S. aureus) for 12 h as described above in the quantitative biofilm formation study.At the end of 12 h of incubation, MCs were removed from the broth and, without rinsing or sonication, immediately placed on dry ice to preserve the morphology of adherent bacteria.MCs were transported to the µ-CT imaging facility where they were kept at −80°C until staining.
Menstrual cups were thawed to ambient temperature for 10 min.Subsequently, cups were exposed to osmium tetroxide (OsO 4 ) vapor for 2 h at 25 °C.Osmium exposure was completed inside an airtight container inside a chemical hood.The proposed underlying staining mechanism and the interaction of OsO 4 with bacteria is an active process of oxidation, which refers to the binding of osmium metal species onto molecules of unsaturated lipids, and other carbohydrates present in the cell membranes via oxidation (61,62).3D x-Ray images were obtained on a µ-CT instrument, Scanco µCT 100HE (Scanco Medical AG, Switzerland).The µ-CT instrument used was a shielded cone beam microtomograph with an x-ray tube with a micro-source and an adjustable focal spot diameter.The x-ray beam passes through the stained menstrual cup, where x-rays are attenuated based on the specimen's osmium accumulation.Hence, the extent of attenuation correlates to the mass density the x-rays pass through.The attenuated transmitted x-rays continue to a digital detector array and generate a 2D projection of the sample.A 3D image of the cup was generated by collecting hundreds of individual 2D projections at different directional angles as the sample was rotated (63).The MC was placed inside a 73 mm low density holder and kept at ambient temperature and relative humidity.The system operated with the following settings: energy level of 45 kVp; 3,000 projections; 55 mm field of view, FOV; and an averaging of 5. Hence, the µ-CT x-ray instrument could acquire a dataset with a high isotropic spatial resolution of ∼20 μm per pixel.
After scanning and subsequent completion of data reconstruction, data was transformed with the AVIZO (Thermofisher, V2020) software for image analysis.Maximum projections were created to identify areas of high osmium concentration and appeared as bright spots on the 3D image.These high osmium accumulation areas were marked and served as a guide during cryo-SEM imaging.
3.4.2.2.2.Cyro-SEM: biofilm tracking of menstrual cup.An area of high contrast within an osmium-stained menstrual cup was excised and securely mounted on a holder with an equal mixture of OCT compound and colloidal graphite.The mounted sample was flashfrozen in liquid nitrogen slush and transferred under vacuum into a Quorum PP3010T cryo-prep chamber.Additional sublimation was performed on the sample at −85°C for 10 min and sputtercoating with a layer of Pt for 100 s to mitigate sample charging.Scanning electron microscopy (SEM) imaging was performed using a Hitachi Ethos NX5000.The use of cryo-SEM is recognized as an invaluable tool in the visualization of biofilm that can reveal details about biofilm structure and topography that other microscopy techniques cannot (64,65).This tool, while limited in use due to the expense of equipment and a skill set necessary to operate the equipment and interpret the images, avoids the complication associated with complex chemistrydriven methodologies (e.g., microscopy such as FISH probes) that require oxygen to work rendering them inconducive to visualization of L. gasseri which requires anaerobic environmental conditions.This experiment consisted of a test cup [artificial soil (as defined below) with cleaning], a positive control cup (artificial soil without cleaning) and a negative control cup (no artificial soil, with cleaning).Prior to the start of the experiment, all MCs were boiled for 5-8 min to ensure a sterile device, consistent with packing insert directions.Artificial soil (e.g., organic matter) was prepared by combining 3 mL of artificial menstrual fluid (AMF) (Q-Labs Cincinnati, OH) and 3 mL of an inoculum of either E. coli (ATCC 8739) or S. aureus (ATCC 33589) in saline.A starting inoculum of 1.0 × 10 8 cfu/ml was chosen for both S. aureus and E. coli to enable evaluation of a sufficient log reduction consistent with available cleaning standards (e.g., FDA).AMF comprised of sterile sheep blood and other proprietary materials was prepared a maximum of 24 h in advance of each experiment; organisms were prepared immediately prior to each experiment.The AMF/organism mixture was vortexed for 1 min and then 500 µl of the mixture was spread via sterile loop on the internal and external surfaces of the test cup and positive control cup and allowed to dry at ambient temperature for approximately 1 h.After sufficient drying, the test cup and negative control cup were cleaned externally and internally for 30 s using lukewarm water and nonscented, non-antibacterial Soft Soap (Colgate-Palmolive, Piscataway, NJ SKU 7418226800 LOT PS001551)) and then rinsed with water.A 30 s cleaning protocol was utilized as it is a timeframe that will most likely elicit the highest compliance rate among menstrual cup users; cleaning intervals longer than 30 s are unlikely to be followed.The test cup and negative control cup as well as the positive control cup, which was soiled but not cleaned, were each placed in a beaker with 200 ml sterile PBS and sonicated for 5 min to remove any adhered organisms (60).After sonication, aliquots were taken from the PBS and plated onto modified letheen agar with Tween (MLAT) agar.Aliquots were filtered through 0.45 µm Sterile gridded Filter Pall Microfunnel 0.45 µm Cat# 4852 as necessary to enable countable range of organisms.The S. aureus and E.coli plates were incubated for a minimum of 24 h at 35 ± 2°C (Figure 3).

Statistics
Analyses were performed in SAS ® 9.4 software, SAS Institute, Inc., Cary, NC, USA.Each data point recovered from the sonicated cups, 200 ml PBS, were normalized against the broth and media control volume of 250 ml VDM before used for statistical analysis.Cell densities for the three cup types were compared to see if there were significant differences in organism recovery between the cups following 12 h of incubation.An ANOVA (analysis of variance) model with cup type as a fixed effect was used to analyze the log count data.Based on these analyses, data from the three cup types were combined and presented as n = 11 in subsequent analyses.Given the nonnormality of bacteria counts, comparisons between the products were performed using log transformed data.An ANOVA model was used to compare the sonicated cup values to the media control value at the 12-h timepoint.For all comparisons, geometric mean ratios and their 95% confidence intervals are presented.All comparisons are 2-sided.

. Understanding potential to introduce oxygen with Tampax menstrual cup insertion
The results of the void volume measurements and available oxygen (mg and mM) in the void volume appear in Table 1.Trace amounts of oxygen were calculated.The C-fold method had the least amount of available oxygen (0.69 mg; 0.0216 mM) compared to the punchdown fold method (1.25 mg; 0.0390 mM).Using the partial pressure from Wagner et al. (50) and the assumptions described in the methods section, time to reach low or zero O 2 was estimated to be approximately 33 min and 1 h for the C-fold and Punchdown fold, respectively.

Clinical study to determine anatomical location of menstrual cup after insertion
Four (4) premenopausal Caucasian women ages 34 through 43 were enrolled in the study after meeting all inclusions criteria and none of the exclusion criteria.Mean BMI score for the group was 29.7 ± 5.3 (range: 24.4-35).Images were captured with the Tampax cup (regular, n = 4 and heavy, n = 3) in situ.Results of the image interpretation (Figure 4) indicate that all MCs that were inserted by participants were placed in the upper part of the vagina encircling the fornices and centered under the cervix.No gaps appeared and the insertion area near the introitus was not held open for any potential air leaks with concomitant ambient oxygen entering the vagina.
The results from both the void volume measurement and the clinical study demonstrated that only a minute concentration of oxygen (C-fold: 0.69 mg and punchdown fold: 1.25 mg) would be introduced in a folded cup.This amount would dissipate quickly via vaginal tissue and would be unlikely to meaningfully change the environmental conditions of the vagina from anaerobic to aerobic.These data provided confidence that the in vitro study should be conducted under anaerobic conditions.A heavy flow Tampax menstrual cup was held upright and filled to the rim with Millipore water.The cup was subsequently folded either using the C or punchdown fold technique causing some of the water to be expelled leaving water only in the void volume (volume remaining after folding).Water remaining in the cup was measured on a 5-place analytical balance to determine void volume (mass/density = volume).The ideal gas law was used to calculate the amount of oxygen that would be present in the cup after folding using the following calculation: Ideal gas law → PV = nRT, where: P = 1 atmosphere, V = void volume in cup (in liters), n = moles of air.R = gas constant (0.08206 L•atm/K•mol), T = 310.15K (body temperature in Kelvin).Subsequently, the time to reach low or zero O 2 was calculated using the starting (131.00 torr) and ending (98.00 torr) partial pressure from Wagner et al. and the following assumptions: pressure remains constant [total pressure at elevated body temperature calculated using Gay Lussac's Law P1/T1 and P2/T2 where P1 is pressure of air at room temperature (1 atmosphere), T1 = 273.15K, P2 is unknown and T2 = 310.15K (body temperature); solving for the unknown (P2) yields 1.13 atm or 862.95 torr at 37°C], the decrease in partial pressure of O 2 increases partial pressure of all other gasses in air, an increase in partial pressure of N 2 and other gasses in air is proportional to volume decreases via P 1 V 1 = P 2 V 2 and the volume decrease is due to absorption of O 2 only.The 3 types of medical grade silicone MCs used in this study were combined for each organism as no statistical differences (p < 0.05) were noted between them in terms of L. gasseri and S. aureus cell density (Table 2).Table 3 summarizes the results from the biofilm formation study following a 12-h incubation in a dual species culture containing S. aureus and L. gasseri.Data from media control showed S. aureus had grown to 10 6 cfu/ml and L. gasseri remained stable at 10 8 cfu/ml organisms.This was expected given the difference in cell densities of the inoculum as well as differences in the doubling time-20 min for S. aureus (66) and ∼150 min for L. gasseri (67),-between the two organisms as bacteria move from the lag phase to the low growth phase.Importantly, the S. aureus cell densities never grew beyond the Cell density values obtained following 12 h of incubation and sonication of the three different cups made of medical grade silicone were compared to understand if there were significant differences in organism recovery between the cups at 12 h.Original count data was log transformed prior to testing.Each data point recovered from the sonicated cups, 200 ml PBS, were normalized against the broth volume of 250 ml VDM before used for statistical analysis.An ANOVA (analysis of variance) model with cup type as a fixed effect was used to analyze the log count data.Additionally, menstrual cups were gently rinsed with PBS before transferred to a beaker with PBS for sonication to release any adhered organisms from the menstrual cup for suspension in PBS.Organisms were plated on selective media under separate and appropriate growth conditions.The number of planktonic organisms recovered from the Broth containing menstrual cups was compared to the number of planktonic organisms recovered from the Media control.Likewise, the number of organisms recovered from the broth was compared to the organisms recovered from the sonicated cup.Each data point recovered from the sonicated cups, 200 ml PBS, were normalized against the broth volume of 250 ml VDM before used for statistical analysis.An ANOVA model with cup type as a fixed effect was used to analyze the log count data.Each experiment contained a broth control for a total n = 16.In some experiments the data obtained from the sonicated cup was out of countable range and in at least 1 experiment, the cup was sent for qualitative assessment via uCT and SEM.Hence, the total n for broth and sonicated cup will not be identical.a Cell density data were log transformed prior to statistical analysis.
L. gasseri recovered from the menstrual cups were consistently 0.5 to 1 log higher than S. aureus.Results from the sonicated MCs were significantly lower when compared to the starting inoculum values (p < 0.0001), 12 h media control (p < 0.0001) and 12 h Broth (p < 0.0001).Although there were no significant differences for either organism when the media control was compared to the Broth, statistically significant differences were noted for both organisms that were recovered from the sonicated cups indicating very few organisms (1.4 × 10 2 cfu/ml S. aureus and 1.3 × 10 3 cfu/ml L. gasseri) adhered to the MC during the time frame of 12 h.
Results presented in Table 4 demonstrate S. aureus growth as a monoculture in the presence of the MC.There was an approximate 2 log increase in S. aureus growth on sonicated MCs when grown as a monoculture vs. when the MCs were grown in the presence of both L. gasseri and S. aureus.These differences show increased S. aureus bacterial attachment to menstrual cup with the same incubation time (12 h) and were significantly different from the results obtained when the test system included L. gasseri 2.10 log 10 vs. 3.96 log 10; p < 0.0001 (Table 4).When the differences between the two inocula (monoculture vs. dual species culture) were examined for the media control, broth, and sonicated cup, all the differences were statistically significant (p < 0.0001) with increased growth in the S. aureus monoculture system (Table 4) demonstrating the important role of L. gasseri in S. aureus growth.

Qualitative evaluation of biofilm formation
Bright areas of high osmium localization in µ-CT images served as a guide to the regions of interest (ROI) where adherent/biofilm bacteria might be found on the MC (Figures 5A,B).Bright signals were detected on the internal and external part/faces of the cup, with bacteria being identified on these surfaces following dual species incubation.Very few organisms were identified on the cup and thick biofilms, as reported in the Nonfoux, et al., could not be found (Figures 5A,B).Analysis of cryo-SEM images differentiated the morphological differences between rods (L.gasseri) and S. aureus (cocci) (Figure 6).Cryo-SEM images confirmed µ-CT observations showing very few adherent organisms were identified on the cup.Importantly, thick biofilms were not identified-only isolated adherent bacteria scattered on the surface were observed.Figure 7 provides visual evidence that S. aureus monoculture with the MC produced similar results, in that no biofilm was present-only a few adherent cocci organisms consistent with the morphology of S. aureus were identified.

Research question #3: Are the current cleaning instructions for the Tampax cup sufficient in removal of adherent bacteria?
Cell counts collected from negative control cups showed no organisms demonstrating that neither the water nor soap contributed to overall bacterial load on the MC (data not shown).Regression analysis of cfu/ml (log 10 scale) estimated log reduction resulting from cleaning MC that had been pre-coated with nAMF, inoculated with 500 ul of 5 × 10 7 cfu/ml each of either E. coli or S. aureus and subsequently cleaned in lukewarm water manually with non-antibacterial, non-scented liquid soap and water for 30 s followed by a brief rinse.Log reductions in both E. coli and S. aureus were >3.5 logs, indicating a >99.9% reduction in bacteria compared to the initial inoculum values: reduction p-values < 0.0001 (Table 5).Mean log reductions between positive control (no cleaning) and test cups that were cleaned for 30 s for both organisms showed above 3 log reduction in bacterial counts (p < 0.05) (Figure 8).The reduction was significantly different from zero (no reduction) when compared to the uncleaned cup (p < 0.05).

Discussion
Silicone materials have been available for use since the 1960s and have played an important and evolving part in products designed for the medical field.Since that time, silicone materials have been developed in various medical grades for skin and mucosal contact, as well as short-term and long-term implantation.Medical-grade silicone molding lends itself to dental and surgical applications, consumer health care products and other medical products and devices.There exists a long history of use of medical grade silicone for fabrication of external and internal devices for the human body (68)(69)(70).The firstknown use of elastomeric silicone, chosen because of its stability and elasticity, was to repair a patient's bile duct in 1946 (68).The material can be cast into virtually any shape (69, 70) and its water-repellant characteristics prevent the material from sticking  aureus biofilm on the cup as well as higher levels of S. aureus growth and TSST-1 production compared to other intravaginal devices.The authors speculate that the menstrual cup introduced a higher volume of air into the test system providing more favorable conditions for S. aureus.Conversely, results from Schlievert's laboratory showed no enhanced S. aureus growth or TSST-1 production compared to no-device controls (73).
No single system of testing is sufficient to answer all possible questions related to risk of toxic shock syndrome, particularly as there is uncertainty about which variables are the most relevant (74).There is also no consensus on how to best test MCs and their constituent components in vitro to determine their potential to increase the risk of S. aureus growth, biofilm formation and TSST-1 production.A variety of testing systems has been described over the years (39, 73, 75) and it should be noted that the Schlievert study most closely followed the original Reiser protocol (75) (see Supplementary Table S-3).Although the Nonfoux et al., article (39) suggested it used the Reiser protocol with modifications, it seems that it only used one component, the WhirlPak bag for incubation with the menstrual cup and bacterial inoculum.It is clear that any system of laboratory testing must be reliable and reproducible and that it should have the power to detect small but significant differences between samples, even at low levels of microbial growth with the potential for subsequent biofilm formation.In vitro methods should not be susceptible to observer bias and should use culture conditions that are physiologically relevant to the human vaginal environment.
Rather than conducting two independent experiments to understand the impact of menstrual cups on S. aureus growth and biofilm production, the same experimental procedure was used to evaluate both endpoints.Nonfoux, et al., showed higher levels of S. aureus growth and toxin production with cups compared to tampons and speculated that the difference may be explained by their manipulation of the MC in the modified Reiser experimental procedure, specifically that a higher volume of air was inserted into the Whirl-Pak bag which has been shown to influence S. aureus growth and TSST-1 production (7,76).Unfortunately, the authors, through placing the MC in a Whirl-Pak bag, failed to mimic the process that a woman uses to fold the MC which decreases the void space prior to vaginal insertion of the MC (Figure 1; Supplementary Table S-3).To address this, the folding and measurement of the void volume of the internal surface was modeled and determined for the amount of oxygen present.MRIs of women who inserted the MC       vaginally and the images indicate that it sits in the fornices surrounding the cervix and not in the introitus where oxygen could possibly enter the vagina during use.Women with diverse BMI scores were included in the clinical study regarding placement of the MC to mimic the scores of the general population of menstruating women (77)(78)(79)(80).
Based on the results of these experiments, an anaerobic environment was selected for testing.This approach is supported by the most recent vaginal oxygen and carbon dioxide values measured during menstruation as reported by Hill, et al., (81).Wagner (50) reported the partial pressure of oxygen within the vaginal lumen before, during and after insertion of a contraceptive diaphragm, which has similar insertion characteristics of a MC.The conclusion reached by the authors in the manuscript is that an increase in oxygen levels within the vaginal canal, due to introducing a pocket of air, creates a favorable condition for oxygen-dependent production of TSST-1 by S. aureus.The authors acknowledge that effects on microbial communities and especially the production of relevant toxin-like proteins was not known or evaluated during the study.In vitro TSST-1 quantification results (7) after a 12-h incubation period demonstrated significant production between 4 and 6 h and a 6-fold difference relative to anaerobic conditions.It should be noted that Wagner (50) only measured oxygen tension for 30 min after insertion which is before the first timepoint in the Schlievert study.Furthermore, electrodes (E524tc; Radiometer, Copenhagen, Denmark) that measured the oxygen were not calibrated and were susceptible to interferences.Most notably, the authors stated "…on insertion of the diaphragm, the luminal PO 2 increased dramatically to 135 mm Hg and then decreased slowly over the next 30 min" which is inconsistent with known pressures for atmospheric gases (160 mm Hg at ambient temperature; 180 mm Hg at 37°C, body temperature).A factual error is made which states "values should be compared with a normal atmospheric PO 2 of approximately 150 mm Hg".The partial pressure of O 2 at sea level is 160 mmHg (82).
Use of dual species culture of vaginal microbes for in vitro assays to address the potential risk of negatively impacting the human vaginal microbiome has been described in the literature (46,47) in response to the specialized testing outlined for tampons (which are Class II medical devices, like menstrual cups) by the U.S. Food and Drug Administration (83).Herein, a novel assay was designed using quantitative and qualitative methods to evaluate the potential for MCs to develop S. aureus biofilms based on experimental conditions inspired by the mixed microflora assay described previously by Weissfeld (84) and Sica (38) including: boiled MCs, media designed to mimic vaginal secretions during menstruation, anaerobic incubation at relevant exposure times (up to 12-h; the manufacturer's labeled maximum time for wear) and dual species culture incubation with a vaginal lactobacillus strain in cell densities reported in the human vagina.Under these experimental conditions, designed to be simple while mimicking as much of the human physiological conditions as possible, highly reproducible results were obtained for S. aureus growth and did not identify the presence of S. aureus biofilm either quantitively or qualitatively.The methods used in these experiments meet the standards required for such an investigation to address the research questions posed at the beginning of this article.
Testing with a single organism (e.g., S. aureus alone) to understand growth in vitro does not allow for evaluating the importance of the interplay between organisms.Numerous, recent publications study growth utilizing more than one organism in a dual species methodology (85-89).Bacterial communities in the human vagina are thought to have a critical role in protecting the host against infectious disease.One of the most important mechanisms of bacteria growth inhibition is based on the presence of a lactic acid producing bacteria.In reproductive age women, it is thought they do so through the production of lactic acid resulting in a low pH environment that restricts the growth of pathogens and other opportunistic organisms (90, 91).The hallmark of vaginal health is correlated to maintaining high number of vaginal lactic acid-producing bacteria.Although there are marked differences in the species composition and rank-abundances of populations in vaginal bacterial communities (12,92,93) among women, it appears that almost all are dominated by homofermentative lactic acid bacteria (93).In the vagina, the keystone species, (e.g., L. gasseri) produces lactic acid through the fermentation of carbohydrates which results in a drop in pH and an inhibition of S. aureus growth.Creating a low pH in the vaginal environment through the production of organic acids may be conserved despite differences in the bacterial species present in the various communities identified in the human vagina.
Multiple publications have noted the specific factors that influence growth inhibition of S aureus when Lactobacilli spp, and specifically L. gasseri, is grown in chemically defined medium.The most prominent reason for the growth inhibition was published by Elkins, et al. (49), indicating that Lactobacillusmediated S. aureus growth inhibition phenomenon was via acidification of the vaginal environment.It could be multifactorial considering some isolated activity in certain strains were observed in spent culture supernatant potentially indicating some bacteriocin-type production.Since the vast majority of lactobacilli required live cultures to exhibit inhibition, this characteristic implies a more general phenomenon indicative of acidity of the environment being the prime driver.Elkins et al. (49), noted that L. gasseri reduced the pH of the medium through fermentation of the glucose in the medium and that other factors were produced, but most specifically focus on the fermentation of the carbohydrates.(Of note, reference 49 utilized the same ATCC strain in their study as was used in in the current study; results showed the L. gasseri dropped the pH.)Boskey et al. (94), showed that during exponential growth, Lactobacillus species acidified CDM growth medium in vitro at rates on the order of 10 6 protons/bacterium(s) supporting the idea that the acidity of the vagina is predominantly produced and regulated by this keystone species.Finally, Dufresne et al. (95), in investigating mTSS using a dual species system of L. gasseri and S. aureus in vitro, noted that increased glucose concentration in a vaginally defined medium could influence S. aureus physiologically when tested in an in vitro vagina model system using a chemically defined medium referred to as VDM.Given this, testing in a dual species system is appropriate when evaluation growth in vitro using VDM.Lactic acid (protonated lactate) has broad antimicrobial activity and vaginal lactobacilli producing lactic acid are known to confer protection against reproductive tract infections when they are predominant in the vaginal microbiota (96).Also, lactobacilli have been noted to produce biosurfactants (97) and Santos, et al. (98), reviewed the presence of microbial biosurfactant which constitute a diverse group of surface-active molecules.They are amphiphilic compounds with both hydrophilic and hydrophobic moieties and with a distinct tendency to accumulate at the interfaces of materials (99).Biosurfactants from various species of lactobacilli have demonstrated anti-biofilm activity against S. aureus strains (100, 101) and have interfered with bacteria and yeasts adherence to silicone rubber (102).Lactic acid bacteria antagonism to S. aureus may act at more than one level by either hampering its growth rate and or by interfering with the modulation of virulence factors such as production of superantigens like TSST-1.Although not investigated in this study, some investigators have noted a modulating effect on staphylococcal enterotoxins/superantigen production in mixed cultures (103)(104)(105).Also noteworthy is that some bacteria produce sophorlipids that have activity against microbial biofilms on medical-grade silicone (106).
Construct validity is the accumulation of evidence to support the interpretation of what a measure reflects.Hence, it concerns the extent to which the test or measure used accurately assesses what it is supposed to evaluate.In research, it is important to operationalize constructs into concrete and measurable characteristics based on one's idea of the construct and its dimensions.The in vitro study designed and utilized in this report is most relevant to address the complexity of studying any potential product/device impact in a complex environment such as the human vagina during menstruation -specifically, the media selection that can mimic the characteristics of human menstrual fluid, anaerobic conditions of the human vagina, paucity of oxygen in a folded cup prior to insertion as well as selection of test strains that have a pedigree of being sourced from the human vagina and prepared in relevant cell densities found in the human vagina.The strains are available through the American Type and Culture Collection (Mannasas, VA, USA) for use by all qualified researchers who apply to obtain them.
The term biofilm describes well established consortium of cells attached to the surface.Formation of biofilms starts with individual cells attaching to the surface, and that stage is usually called initial attachment, and it may lead to biofilm formation.The quantitative and qualitative data from the S. aureus biofilm formation study presented in this paper demonstrated that: (1) very few organisms of the mixed culture adhered to the MC; (2) S. aureus was rarely identified and;(3) the predominant morphology of adherent bacteria were rod-shaped bacteria consistent with the morphology of L. gasseri used in the test system.Biofilm formation is a social group behavior.Each of the steps from initial attachment to the dispersion and transmission of mature biofilm is strictly controlled by multiple regulatory systems or regulators and to develop a young biofilm in vitro may take several days (107, 108).It should be noted that biofilm formation was not observed by either of the two test organisms on the MC during SEM visualization.These data support the importance of the presence of L. gasseri in controlling the growth and adherence of S. aureus to the MC.It is hypothesized that the results from this in vitro system are similar to what would occur in the human vagina when using a MC during menstruation.It is suggested that any future studies involve gentle movement of the test systems to model human movement.
MCs are a comfortable, safe (when used as directed) and efficient option for menstrual hygiene (109).They are marketed in almost 100 different countries and are viewed as a meaningful alternative for use compared to disposable menstrual hygiene products.Different MC brands recommend a variety of techniques for cleaning in-between uses and storage from month to month.Overall, the cleaning directions vary and range from wiping the cup to boiling for 10 min (110).As noted in the same 2021 report, "[m]anufacturers should standardize cleaning terminology to internationally agreed upon definitions" as the terms sterilization, disinfection and sanitization are used interchangeable and inconsistently in directions for caring for the MC (111).The term "cleaning" may be more appropriate for the MC as it involves removal of visible organic and inorganic material from objects as well as a significant number of bacteria from surfaces that is normally accomplished manually using water with detergents.Cleaning is a necessary step prior to subjecting devices to boiling.The methodology described herein utilizes slightly different design parameters than described by Wunsch (40) to understand cleaning effectiveness including use of an artificial menstrual fluid, meant to represent the complex nature of menses (Supplementary Table S-4).The data described herein have demonstrated that, when the cleaning protocol was initiated, the soiled MC with added bacteria using model gram positive and gram negative organisms, had at least a 3 log reduction (99.9%) when S. aureus and E. coli were inoculated on the MC compared to study initiation at t = 0 h.Less than 200 cfu/ml from the MC were identified as part of the sonication protocol.It can be concluded that few organisms adhered to the MC following the cleaning protocol using unscented non-antibacterial soap, water, and manual scrubbing with fingers (and subsequent rinsing) for at least 30 s.These data suggest cleaning the MC with soap and water is an effective technique at removing bacteria between uses during the menstrual cycle.This degree of bioburden reduction (99.9%) achieved in this study is considered the gold standard for cleaning single-consumer reusable devices (43) and has been deemed acceptable by regulatory authorities (112) in the clearance of other reusable, intravaginal devices (e.g., Fem Cap) similar to the menstrual cup.
The processes governing bacterial adherence and subsequent biofilm formation are rather complex, involving several steps under the right environmental conditions and exposure time.When all these co-factors are met, almost all medical device surfaces are susceptible to colonization by adherent bacteria during human use (99,113).Nevertheless, the results presented from our experiments do not support Nonfoux, et al. (39), regarding S. aureus biofilm formation on MC or Wunch et al. (40), regarding cleaning the MC.No visual identification of any biofilm formation by S. aureus using cryo-SEM was observed a well-accepted methodology for visualizing microbes and their modality of growth.As summarized by Schlievert in the 2020 in vitro study, "…devices such as diaphragms, menstrual discs, and menstrual cups, which are not absorbent, do not increase TSST-1 production vs. no-device controls".Given this, Schlievert suggests that the rare reports of mTSS with the MC may be more coincidental than as a risk factor for the disease when the device is used as recommended (73).It is clear, based on these studies and previous reports that the current recommendations to care and manage the Tampax MC between uses are appropriate and provide consumers with an easily accessible method for cleaning the cup between uses during the menstrual cycle.The conclusion is that this work provides data counter to data published by Nonfoux et al. (39), that called into question the safety of the Tampax ® cup during use by menstruating women.Based on the experimental results provided herein, the data support the continued safe use of the Tampax ® cup when used and maintained as recommended.

3. 4 . 3 .
Research question #3: Are the current cleaning instructions for the Tampax cup sufficient in removal of adherent bacteria?

FIGURE 4
FIGURE 4 Magnetic resonance imagining of the Tampax menstrual cup in situ, sagittal view.MRI image T2 (transverse relaxation time) sagittal image of the menstrual cup in the female reproductive tract.Menstrual cup in situ contacts cervix and vaginal walls.Green: menstrual cup, blue: vagina, yellow: cervix, red: vaginal fornices, orange: uterus.

FIGURE 5
FIGURE 5 Contrast enhanced 3D x-ray imaging of Tampax menstrual cup using micro-CT.(A): Post-processed Tampax menstrual cup x-ray scan.By implementing morphological operations on the 3D object representing the menstrual cup, inner and outer surfaces were segmented.After segmentation, maximum projection, of both inner and outer surfaces, were acquired from top and side views [top row areas: approximate (40 mm, 37 mm)].Bottom row represents zoomed-in regions of interest [orange squares of top row approximate areas: (11.8 mm, 12.5 mm)] showing high intensity areas (bright areas indicated by yellow arrows in bottom row) of high osmium accumulation, which areas are proxy locations for SEM imaging.(B): Post-processed menstrual cup x-ray scan.A maximum projection of the whole 3D object representing the menstrual cup.Regions of interest (ROIs, orange and green squares) were identified where high osmium accumulation was present as bright local areas (With Bacteria Sample, ROIs Orange Left [7.6 mm, 12.9 mm], Orange Right [10 mm, 7.7 mm], Green Center [3.6 mm, 3.95 mm]; Without Bacteria Sample, ROIs Orange Top [5.9 mm, 7.1 mm], Orange Bottom[4.7 mm, 4.8 mm], Green Center [2.35 mm, 4.8 mm]).3D renderings of ROIs were created to depicted 3D structure and topology of high osmium accumulation per sample, with and without bacteria.

Friberg
to wounds.Menstrual cups first became clinically available in the 1950s (71, 72).A recent review on MCs summarized available published research and concluded that they are a safe option for menstruation management and are being used internationally (37).Recent publications, by Nonfoux et al. (39) and Schlievert (73) have investigated MCs that are composed of this material in in vitro test systems to determine the impact of the device on S. aureus growth and TSST-1 production and/or biofilm formation.Results from Nonfoux et al. showed formation of S.

FIGURE 7
FIGURE 7Cryogenic scanning electron microscopy images of tampax menstrual Cup following 12 h incubation with S. aureus alone.Low magnification cryo-SEM images of excised silicone menstrual cups of S. aureus (red arrowhead).

FIGURE 6
FIGURE 6 Cryogenic scanning electron microscopy images of Tampax menstrual cup following 12 h incubation with S. aureus and L. gasseri.(L-R): Low magnification cryo-SEM images of excised silicone menstrual cups (left image); higher magnification cryo-SEM images of lactobacillus (middle image, green arrowhead) and S. aureus (right image, yellow arrowhead).
SE, standard error; CI, confidence interval.Approximately 500 ul of nAMF (new artificial menstrual fluid) inoculated with 5 × 10 7 cfu/ml was applied to the menstrual cup and allowed to air dry at ambient temperature for approximately 1 h prior to testing Positive control cups (n = 4 and n = 5 for S. aureus and E. coli, respectively) were not cleaned; test cups (n = 8 and n = 9 S. aureus and E. coli, respectively) were cleaned for 30 s with a non-scented, non-antibacterial soap.An ANOVA model with cup treatment as a fixed effect was used to estimate the log reduction resulting from cleaning the menstrual cup with non-antibacterial soap and water.*P < 0.05.

FIGURE 8
FIGURE 8Cell density reduction of S. aureus and E. coli on the Tampax cup after 30 seconds cleaning (cfu/ml).Mean log reduction between positive control (no cleaning) and test cups (30 seconds cleaning with non-scented, non-antibacterial soap.Mean log reductions are above 3 logs, indicating a 99.9% reduction in bacterial count for both E.coli and S.aureus.p-values are well below 0.05, indicating that the reduction in bacterial count is significantly different from zero (no reduction).

TABLE 1
Estimate of amount of oxygen in void volume of Tampax menstrual cup based on ideal gas law calculations and calculation of time to reach zero.

TABLE 2 Sonicated
Tampax cup cell density comparisons following 12 h incubation.

TABLE 3
Media Control and Menstrual Cups were anaerobically incubated over 12 h in VDM-PS containing S.aureus + L.gasseri.At 12 h, samples were collected for plating and enumeration from the Media Control and Broth of each Test Product.
Broth cell density comparison (mean cfu/ml) from Media control, broth, and sonicated Tampax cup after 12 h stagnant incubation in a dual Species culture under anaerobic conditions.

TABLE 4
Comparison of S. aureus cell density following 12 h incubation in a mono-culture vs. dual species culture.
a Dual Species culture contained both S.aureus + L.gasseri; monoculture contained S.aureus alone.b Count data were log transformed prior to testing.An ANOVA model with fixed inoculum effect was used for analysis.c Mean(cfu/ml) uses original scale.