Conquering the World: The Invasion of the Red Swamp Crayfish

One of the biggest threats to biodiversity is the invasion of non-native species, also called invasive alien species. It is crucial for scientists to determine the main introduction routes by which non-native species enter into an ecosystem. We studied the red swamp crayfish (Procambarus clarkii), a freshwater crustacean, and looked at the genetic characteristics of 122 crayfish populations (22 native and 100 introduced) to understand their differences and where they came from. Our results showed a great amount of genetic variability among crayfish populations and that, this crayfish species has invaded different parts of the world via multiple introduction events and pathways, with humans playing a key role in these introductions. It is therefore vitally important to control the likely sources of invasion to avoid further introductions and prevent the rapid expansion of crayfish populations.

One of the biggest threats to biodiversity is the invasion of non-native species, also called invasive alien species. It is crucial for scientists to determine the main introduction routes by which non-native species enter into an ecosystem. We studied the red swamp crayfish (Procambarus clarkii), a freshwater crustacean, and looked at the genetic characteristics of crayfish populations ( native and introduced) to understand their di erences and where they came from. Our results showed a great amount of genetic variability among crayfish populations and that, this crayfish species has invaded di erent parts of the world via multiple introduction events and pathways, with humans playing a key role in these introductions. It is therefore vitally important to control the likely sources of invasion to avoid further introductions and prevent the rapid expansion of crayfish populations.

WHAT ARE INVASIVE ALIEN SPECIES AND WHY ARE THEY A PROBLEM?
We live in a changing world, where we can travel over great distances more easily and quickly than ever before. Humans have accidentally, or intentionally, transported lots of species out of their natural homes, or native ranges, for centuries. However, the frequency of such severe problems for the environment, the economy, and human well-being. For example, the accumulation of zebra mussel (Dreissena polymorpha) shells in water intake pipes used for irrigation of fields can block the pipes and cause the farmers to lose large amounts of money. As another example, the digging activity of a fish called the common carp (Cyprinus carpio) increases the cloudiness of the water, which can reduce the ability of aquatic plants to perform photosynthesis by decreasing the amount of light that reaches them. Given their serious impacts, invasive alien species are considered one of the greatest threats to biodiversity, ecosystem services and human well-being.
Scientists are constantly asking questions about biological invasions. Why are some species successful while others fail? [ ]. How are invasive alien species introduced? Why do they become invasive? Where are they distributed? [ ]. Researchers know that the invasion process consists of several stages. The success of their establishment often depends on the number of individuals released and how they are introduced. Usually, there is a greater chance of establishment if hundreds of individuals are introduced multiple times into a specific site than if there is one single introduction event involving only a few individuals [ ]. Therefore, understanding why these species are introduced, where they came from, how they spread, and how the invasion process works are all key factors in determining the best way to prevent the establishment or spread of invasive alien species and decrease their negative impacts worldwide [ , ].

THE INVASIVE RED SWAMP CRAYFISH (PROCAMBARUS CLARKII)
The red swamp crayfish (Procambarus clarkii) is native to the southern United States and north-eastern Mexico, but can now be found in inland waters on all continents except Australia and Antarctica [ ]. This freshwater crustacean easily reaches high densities, become an invasive species, and it causes severe ecological and economic impacts, such as preying upon native plants and animals, transmitting diseases to other aquatic species, and damaging dykes and canals in rice fields due to its burrowing activity ( Figure ). But how has the red swamp crayfish become a widespread invader? The introductions of crayfish have mainly been due to its economic value as seafood [ ]. Yes! Eating freshwater crayfish is common in many countries (for example, there are Crawfish Festivals in the United States and Sweden) and generates tens of billions of U.S. dollars per year around the world. Due to a lack of awareness of its impacts, and hoping to make some money, people have intentionally introduced this species into the wild in many locations worldwide.
Since the red swamp crayfish has an important commercial value, many introductions to new areas have been well documented by scientists, crayfish enthusiasts, or commercial companies [ ]. For example, when crayfish were introduced into Spain, the person who brought them into the country, the precise date and place of introduction, and the number of crayfish introduced were all recorded. However, it is uncommon to have such detailed information, so genetic tools are needed to unravel the invasion history for most invasive species. In the case of the red swamp crayfish, we did both: we used the available historical information and genetic tools to confirm the invasion routes of this organism.

HOW THE RED SWAMP CRAYFISH TOOK OVER THE WORLD
The first known introductions of the red swamp crayfish into new locations took place in the s: in California and in the Hawaiian Islands in United States, in Japan, and in China. In the mid-s, a batch of crayfish was sent to Uganda and Kenya, and soon afterwards, to other African countries. Simultaneously, these animals spread over Mexico and reached Costa Rica, Puerto Rico, Venezuela, and the Dominican Republic in the s, and Brazil in the mid-s. The red swamp crayfish was legally introduced into Spain in and , first from Louisiana and probably later from Africa or south-east Asia. Currently, this species is present in at least countries worldwide [ ]. As you can see, the red swamp crayfish has traveled a lot! By using this information and collecting crayfish from di erent places around the world, we were able to uncover its invasion process, describe its genetic variability, and understand its global invasion patterns.

COLLECTING CRAYFISH THROUGHOUT THE WORLD
We collected a total of , red swamp crayfish from locations in North America, East Asia, and Europe. In the laboratory, we isolated a type of DNA that is commonly used to compare populations of animals, called mitochondrial DNA (mtDNA), from a small piece of MITOCHONDRIAL DNA (mtDNA) DNA located in the mitochondria, which are cellular organelles within many types of cells. muscle tissue from each crayfish. We also obtained genetic sequences from crayfish populations in China and Mexico, which had been determined by other researchers, and we compared these with my samples, too. Like all DNA, mtDNA is made up of a string of four molecules, called nucleotides, which are arranged in a specific sequence. DNA sequences are passed down from our parents, and mtDNA sequences always come from our mothers. We selected a short fragment of the mtDNA sequence that we could compare between crayfish locations, to see how genetically similar these animals were. The specific mtDNA sequence of each individual is called its haplotype. Crayfish with the exact same mtDNA sequence HAPLOTYPE Sequence of nucleotides in a certain region of the mtDNA. are said to have the same haplotype, meaning they are more closely related to each other. If an organism di ers by even one nucleotide, it is said to have a di erent haplotype. So, if two populations of crayfish in di erent areas of the world (for example, one from China and other from United States) share the same haplotype, there is probably a connection between these two locations, and we can use that genetic connection, together to more technical information, to figure out where the crayfish came from. In this way, we were able to reconstruct the invasion routes of red swamp crayfish around the world.

DIFFERENT INVASION PATTERNS WERE FOUND
The , crayfish sequences we studied included di erent haplotypes, which di ered by at least one nucleotide (Figure ). Generally, in the ecosystem that is native to an organism, we ECOSYSTEM Community of living organisms interacting with non-living elements of their environment as a complex system. should find all haplotypes, but populations that have been introduced into new non-native environments usually show a smaller number of haplotypes because a few individuals are usually transported. However, the haplotype variability in an invasive species population can vary for a number of reasons, and this is what we found when we looked at the haplotypes of red swamp crayfish.
One thing we learned was that invasive crayfish populations from the western United States had higher haplotype variability and were very di erent from the invasive populations found in the eastern United States. This is probably because crayfish were introduced multiple times into the western of United States, because there were Reconstructing the Global Invasive Routes Figure   Figure Haplotype sequences of several red swamp crayfish. A fragment of the mtDNA sequence that we selected for comparison is shown at the top of the matrix. The dotted lines underneath represent crayfish sequences from this same location in the DNA. Letters are shown in place of dots in cases where di erences in the nucleotide sequence, representing di erent haplotypes of crayfish, exist. In this picture, di erent colors represent the four nucleotides of DNA: guanine (G, black), thymine (T, red), adenine (A, green), and cytosine (C, blue).
Yellow arrows indicate that the crayfish shown in line numbers , , and belong to three di erent haplotypes than do the other crayfish studied. several crayfish supply companies in California that sold live crayfish and end up being released to wild. In the eastern United States, however, a sole company, Carolina Biological Supply Company, was predominant. We also saw low haplotype variability in Asian crayfish populations, which confirmed the invasion history in East Asia. That is, according to the literature, crayfish were carried from New Orleans to Japan in , of which only crayfish survived. In Europe, the highest haplotype variability was found in southern Spain, where the initial introduction of crayfish occurred, and the haplotype variability decreased northwards, as new populations grew from a few individuals transported from the original southern population. However, we found high variability in other European populations, which is probably due to multiple introductions, as explained before. Additionally, we saw a di erent haplotype in parts of northern Europe, like France, the United Kingdom, Belgium, or The Netherlands, that was not found in southern Spain where the initial introduction occurred. This suggested that other, undescribed introduction routes could have taken place in northern Europe.

TAKE-HOME MESSAGE: CRAYFISH INVASION IS MORE COMPLEX THAN WE THOUGHT
The red swamp crayfish, just like lots of other invasive species, has been introduced into new locations by humans for many di erent purposes. Through our work, we described the complex invasion of the red swamp crayfish in the northern hemisphere, highlighting the key role of humans in its movement from one place to another (Figure ). In recent decades, the red swamp crayfish has been continually introduced in even more locations, which has resulted in its rapid spread all over the globe. Depending on the number of introduction events and the number of individuals introduced, we found invasive populations with low genetic variability (only a few haplotypes) or high genetic variability (multiple haplotypes). Overall, these findings are crucial for understanding how invasive species expand. This knowledge can help us find more e ective strategies to  prevent their spread and the negative e ects that can result when they take over an ecosystem.

ACKNOWLEDGMENTS
I am hugely grateful for the comments and suggestions received from T. C. Michot, L. Boyero, M. I. Sánchez, and M. Ferraguti who improved the first draft of the manuscript. I thank the two young reviewers for their comments and suggestions, their two respective mentors and the editors, Pedro Morais and Susan Debad, for their valuable advices to improve this manuscript and making it more suitable to a young audience.
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