How Can Studying Mucus Protect Coral Reefs?

Corals are an association between animals and plants that deposit a skeleton and build reefs. Coral reefs are now in danger because the marine environment around them is degrading, causing corals to get ill more often. Scientists have used fragments of healthy coral colonies or planted baby corals to help restore reefs that are in bad shape. But for these efforts to succeed, it is vital for the scientists to select healthy corals for planting. Corals use a substance called melanin to trap bacteria that grows in their tissues. We discovered that the enzyme phenoloxidase, which is responsible for making melanin, can be found in the snotty covering of corals, called mucus. We can take samples of this mucus to measure phenoloxidase without harming the corals. By doing so, we can detect whether a coral colony is responding to the presence of harmful bacteria even before it shows any signs of illness.

Corals are colonies of polyps that are interconnected. Each coral polyp is a small, soft-bodied sac, with a mouth surrounded by tentacles that are used to feed. Each polyp produces and sits on a calcareous skeleton. Corals grow into various forms. The whole colony is covered by a mucus sheet for protection.

WHAT ARE CORALS?
Corals are not plants or rocks, but animals that live in tropical seas. Have you ever seen an anemone? Well, the polyps of corals are very POLYPS Soft, slimy animals in the shape of a pear, longer than wider. In the apical part are surrounded by tentacles that serves to feed themselves. Individually, are cnidarians known as anemones, but when form colonies, are known as corals. similar to tiny anemones. In corals, many polyps group very close together to form a colony, and all those polyps are interconnected. Coral polyps have tentacles surrounding their mouths that help them to feed themselves, by trapping small animals from the surrounding water. The tentacles also help the polyps to eat the bacteria that grow on the surfaces of their bodies ( Figure ). Coral polyps sit on rocks that they make from calcium carbonate, which is called the calcareous skeleton. The calcareous skeleton is composed of calcium (a mineral

CALCAREOUS SKELETON
An external skeleton that corals create and then grow on top of, composed of calcium carbonate (CaCO ). Due to this skeleton, corals can build reefs, some of which are so big that they can be seen from space. that gives structure to our bones and teeth), and carbon (the main component of carbon dioxide, a gas that belongs to the greenhouse gases). Corals produce the calcareous skeleton by removing carbon dioxide and calcium from the water.
The calcareous skeletons of corals form big structures, better known as reefs, that house many other marine organisms. Coral reefs also protect the people and communities that are close to the coast, because reefs can reduce the energy that comes from hurricanes by up to %. Thus, corals are not just beautiful marine organisms, but they are also very important for other marine species and for human beings.

CORALS CAN GET DISEASES!
Corals, like every animal on this planet, can get diseases. When corals get sick, the color of their surface changes. For example, corals with black band disease lose some of their surface tissue, leaving a bright white skeleton free of polyps. The main sign of black band disease is the development of a band through the colony, which may be black, red, or brown. There are di erent kinds of diseases, such as white or yellow band disease or dark spot disease. Once signs of disease are seen in corals, the disease may spread rapidly through colonies, and in time, the whole colony may die. But what can these animals do to protect themselves from getting sick?

CORALS HAVE AN IMMUNE SYSTEM
Even though corals look like simple animals, they have a complicated immune system. This system has mechanisms capable of healing

IMMUNE SYSTEM
An organism's system for fighting or resisting the growth or invasion of harmful organisms to prevent disease. injuries, killing or engulfing harmful microorganisms, and helping corals to stay healthy [ ]. Like humans, corals secrete a snotty layer called mucus that protects and separates their tissues from the surrounding seawater. The mucus layer plays an important role in the immune system of corals. It contains substances that can battle harmful bacteria that might otherwise cause some coral diseases. In humans, mucus works similarly, covering our guts and the insides of our noses and throats. If this mucus layer is breached, our inner tissues, like the coral tissues, can get infected.
Corals also produce a substance called melanin to trap harmful bacteria and other microorganisms. Melanin is made by specific enzymes called phenoloxidases (PO). An increase in the activity of ENZYMES Molecules in the body that help convert one substance into another.

PHENOLOXIDASE
The enzyme responsible for producing melanin. Phenoloxidase is part of the immune system of corals, and when it is being synthesized, other toxic substances are also produced.
PO can tell us if the coral has started to respond to the presence of bacteria [ ]. When PO is being produced, some toxic substances are also produced, which can kill harmful microorganisms. In humans, melanin gives color to our skin. But in corals and anemones (relatives of corals) that have been infected by harmful bacteria, melanin forms darkened clots in their tissues [ , ].
When many corals are damaged or die, the reef loses many of the benefits it provides. In e orts to rescue corals from deteriorating reefs [ ], scientists sometimes use fragments of healthy coral colonies or plant baby corals on the damaged reefs. To do this successfully, the scientists must first make sure that the colonies they select to repopulate those reefs are healthy. This is why we decided to take a look into the mucus layer of healthy and sick coral colonies, to see if we could predict whether healthy looking corals were actually healthy or just not showing signs of illness yet.

COLLECTING CORAL MUCUS
To get a better understanding of the health of coral mucus, we dove down to the reef near our laboratory and, with the help of syringes without needles (one syringe per coral), we carefully sucked up the mucus from the surfaces of healthy and diseased corals. This technique is safe for the corals because it does not damage them at all.
Do you remember how we said that, when corals get diseased, they lose some of their tissues and the diseased areas can be bands or spots with a di erent color than the healthy coral? Well, when we took the mucus from diseased corals, we collected it from two di erent parts of the colony: ( ) over the damaged tissue (where the coral tissue had changed color) and away from the damaged tissue, over healthy-looking tissue. In healthy corals, we just took mucus from one part of the colony (Figures A,B). Each syringe was labeled so we knew where the mucus came from. Then we took the mucus samples to our laboratory.
Back in the laboratory, we tested the mucus samples by adding substances that stimulated the PO enzyme to make a colored product ( Figure C). We measured the quantity of the colored product in each sample with a spectrophotometer. A spectrophotometer is an SPECTROPHOTOMETER Laboratory instrument that measures chemical components dissolved in a liquid, by shining a ray of light into the liquid and reporting how much light is absorbed.
instrument that shines a ray of light through a liquid sample. In our case, the more light the sample absorbs, the more PO activity there is.

WHAT DID WE FIND?
As we mentioned earlier, coral polyps release immune substances into their mucus. Our first discovery was that the PO activity could be measured in the mucus [ ], which is very convenient because, as we mentioned, we can take mucus samples without harming the corals. The second discovery was that there was di erent PO activity in the mucus samples from healthy and diseased corals. The mucus from healthy corals contained a small amount of PO compared with the mucus from diseased corals. Also, the samples from the diseased corals taken from over the damaged tissue contained the highest quantity of PO, compared with the samples taken further away from the damaged area (Figure ). Therefore, we discovered that PO activity in mucus is well-correlated with infection: areas with infection have more PO, and therefore more activation of the immune system, than healthy areas.

WHAT CAN WE DO TO HELP CORALS?
The next step in this research is to determine the normal level of PO activity present in mucus from various healthy coral species. PO activity in the mucus can tell us about the coral's health. Then, when scientists want to collect larvae or fragments from healthy kids.frontiersin.org October | Volume | Article |

Rivera-Ortega and Thomé
Corals Defenses in Mucus

Figure
PO activity in the brain coral Pseudodiploria strigosa is a ected by black band disease. In this plot, the three bars show the PO activity present in the healthy mucus samples (yellow), and in the mucus samples from diseased corals, taken a distance away from the damaged area (purple), and over damaged tissue (green). The plot shows that the activity of PO is low in healthy colonies but increases to medium levels in mucus taken from healthy looking areas of diseased colonies and is highest in samples taken over damaged coral tissue.
coral colonies to help rescue diseased coral reefs, they can test the apparently healthy colonies first, to make sure they are healthy, before they are used in the rescue e orts. Saving sick coral reefs with healthy coral fragments can contribute to the preservation of this extremely important marine ecosystem.

CONFLICT OF INTEREST:
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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