Looking for Earthworms in Deadwood

Fallen branches, logs, and tree stumps are a valuable habitat in forests, giving food and shelter to many organisms, including earthworms. Unfortunately, deadwood is often removed from forests because its value is not fully appreciated. We wanted to overcome this by developing a sampling method for earthworms living in deadwood in forests. By testing our new sampling method in oak forests, we found that including deadwood in earthworm surveys improves our knowledge of forest earthworm populations. We also found a greater number of young earthworms in deadwood, where conditions were warmer and moister than in the soil. By surveying deadwood for invertebrates, such as earthworms, we can better understand the important role that deadwood in forests plays in maintaining biodiversity.

An epigeic earthworm found in deadwood. The pale band (or "saddle") toward the head end (to the right) tells us this is an adult earthworm. plant material, recycling its nutrients back into the environment. But earthworms do not just live in the ground, they can be found in all sorts of unexpected places-even up in trees and inside logs! Logs and fallen trees are called deadwood and are an important DEADWOOD In forests, woody material that is no longer living, including fallen branches, logs, stumps, and standing dead trees.
habitat for many di erent types of earthworm, giving them shelter and food (Figure ). There are three main groups of earthworms: epigeic (living above-ground), endogeic (shallow-burrowing in soil), EPIGEIC Earthworms that live on the surface of the soil in organic matter-rich habitats, like leaf litter. the remains of organisms, such as plants), and its decomposition is important for maintaining healthy forest soils.

DEADWOOD IS AN IMPORTANT BUT UNDER-STUDIED HABITAT
Despite being a key source of soil organic matter and an important habitat, deadwood is often removed from forests when trees are cut down for timber and firewood, putting many species of animals at risk of going extinct [ ]. Protecting forest biodiversity is important.

BIODIVERSITY
The variety of life in a habitat, a high level of which is usually desirable.
Biodiversity is the variety of life in a habitat, and we depend on the services that healthy and highly biodiverse forests provide, such as storing carbon and protecting soil. The more information we can learn about the amount of biodiversity present in deadwood, the better we can understand the importance of keeping it in forests. We do not have a full idea of which earthworms live inside deadwood, because we do not currently have a scientific way of looking for them there. Earthworm surveys normally only look in the soil and may miss earthworms living in other places. By developing a way to survey deadwood for earthworms, we can learn more about earthworm lifecycles and perhaps show how important it is to keep deadwood

SURVEYING DEADWOOD FOR EARTHWORMS
To try out our method, we visited forests of common oak trees (Quercus robur) in Surrey, UK, and in each forest, we marked out a × m square plot ( Figure ). In each plot, the total volume of deadwood was recorded and we tried to determine which tree species the deadwood belonged to. We also estimated how decayed the deadwood was, based on a -to-ranking system, in which is least decayed (freshly fallen) and is most decayed (deadwood completely broken down into the soil). Since small branches have very few earthworms in them, we selected five pieces of deadwood that were larger than cm in diameter, and of mid-to late-decay stage (loose bark and wood beginning to soften). We analyzed the deadwood by placing it onto a sheet and measuring its length and diameter, so we could calculate its volume. Deadwood temperature was measured by inserting a digital kitchen thermometer beneath any loose bark. We then removed any moss and loose bark and inspected it for earthworms, and the remaining wood was taken apart and inspected. Once all earthworms were collected, the deadwood was then returned to its original location, with moss and loose bark replaced as best possible.

SURVEYING SOIL FOR EARTHWORMS
We also sampled the soil for earthworms using a standard method. This involved digging soil pits ( × cm wide and cm deep) Looking for Earthworms in Deadwood at five locations in the plot (Figure ). First, we placed the soil onto a sheet to hand-sort for earthworms, and then we poured L of mustard water ( g of table mustard powder mixed with L of water) into each soil pit, to extract deep-burrowing earthworms, some of which can dig m deep! This mustard powder irritates the earthworms' skin and encourages them to come up to the surface. Soil moisture and temperature measurements were also taken. Once all earthworms were collected, the soil was placed back into the holes. All earthworms from deadwood and soil were preserved in alcohol and were weighed, then each species was identified using a microscope and an earthworm identification guide [ ].

WHAT DID THE SURVEYS REVEAL?
Overall, we found a total of , earthworms and di erent earthworm species. The number of earthworm species was di erent between soil and deadwood, with seven species found in deadwood, and twelve species found in soil ( Figure ). One species, Eisenia fetida, was found only within deadwood. This is an epigeic earthworm that is often found in compost heaps. Six species were found only in the soil: two epigeic, three endogeic, and one anecic species. All other earthworm species were found in both deadwood and soil. Many more juvenile (young) earthworms were found in deadwood than soil, and deadwood was much moister than the soil and warmer by around • C.

BIOMASS
The mass of an organism or community of organisms in an area or ecosystem.
(mass of all earthworms) was much greater in soil than in deadwood ( Figure ). On average, the deadwood surveys contributed an additional earthworms and g earthworm biomass per m forest plot.

THE BENEFITS OF DEADWOOD SURVEYS
By adding deadwood to our forest earthworm surveys, we found both a greater abundance of earthworms and more earthworm species than we would have found by doing only soil surveys. There was a much greater proportion of juvenile earthworms inside deadwood, where temperature and moisture conditions were more favorable. Earthworms are very sensitive to temperature and moisture extremes, so being protected in decaying wood allows earthworms to stay active throughout the year, especially during summer drought and freezing winter conditions. Removing deadwood from forests may therefore have a negative e ect on the many earthworm species that rely on it for shelter and food. Based on our results, we can say that forest-based earthworm research that does not include deadwood is likely to underestimate earthworm populations, and forests without deadwood will have fewer earthworms. With more development, our survey method could be used for studying other important invertebrates that live in deadwood, such as insects [ ]. Hopefully we can use this information to improve forest management practices, to make sure that deadwood is left in place to protect forest biodiversity. . "Strategies lombriciennes," in Organisms as Components of Ecosystems, eds U. Lohm and T. Person (Stockholm: Ecological Bulletin). p.

CONFLICT OF INTEREST:
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
COPYRIGHT © Ashwood, Vanguelova, Benham and Butt. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. and what e ect the changing climate is having on their condition. For this I study all aspects of a tree's environment, from the soil around its roots to the air around its leaves and the animals that rely on it.

KEVIN R. BUTT
Kevin is an ecologist at the University of Central Lancashire. He has studied earthworms for more than years and is interested in how these ecosystem engineers are able to assist humankind through their everyday activities, such as burrowing and eating organic matter. He has studied earthworms throughout Britain and undertaken research across Europe and in the USA. One of his current projects is examining the action of earthworms on Charles Darwin's estate, recreating experiments of the great scientist, whilst another is investigating giant rainworms in the mountains of Germany.