Tiny Fungi in the Soil Are Like Medicine for Nature

Like humans, plant and animal species have a specific type of place, called an ecosystem, where they prefer to live. If the ecosystem changes too much, some species will disappear, much the same way people avoid living in the desert because it is too hot and dry. Humans modify many ecosystems, sometimes so severely that almost no plants or animals can live there anymore. To help damaged ecosystems recover, we often start by planting trees or other plants. Biologists found that mycorrhizal fungi, tiny fungi living in the soil and inside plant roots, could speed up ecosystem recovery by making plants grow back faster and stronger. In this article, we describe how the recovery of ecosystems can be enhanced by mycorrhizal fungi, and when mycorrhizal fungi are especially helpful.

many functions and serve as homes for native plants, animals, and tiny microorganisms in the soil. Some ecosystems also filter and store water, keep excess carbon out of the atmosphere, and preserve a healthy soil layer where plants can grow and produce fruits and vegetables for us to eat. Ecosystems provide these functions if they are healthy and intact, but sometimes ecosystems become disturbed and stop functioning well. Such disturbances include fires, floods, or tree fall after storms. Fortunately, healthy ecosystems can usually recover easily from these disturbances. However, if disturbances occur frequently over a long period, or if they are very intense, ecosystems have more di culty recovering and might change. For example, a forest might become a grassland after a very intense storm pulls all the trees over. As another example, after a long period of drought (when there is not enough water for plants to survive) plants may have a hard time growing back even when water is available again. If, after disturbances, ecosystems change to become less complex and have fewer species living in them, we call them degraded ecosystems.

DEGRADED ECOSYSTEM
An ecosystem that has been damage or destructed, which also negatively a ects the organisms living in the ecosystem and hampers the functioning of the ecosystem.
Humans use ecosystems to create space for cities, to produce food in agricultural fields, or to mine for stones, sand, or metals for building or manufacturing. These human activities often disturb ecosystems so severely and for so long that the ecosystems are pushed over the edge from where they can no longer recover without help. Ecologists, which are scientists who study how ecosystems function ECOLOGIST A scientist that studies how ecosystems function, and how the organisms in an ecosystem interact with each other and the environment. and can be home to many species, can help restore degraded ecosystems, helping them to regain their health and their functions. When ecologists restore ecosystems, they might plant trees to help forest recovery after trees are cut down for timber production, or sow seeds to help grasslands recover after they have been used as agricultural fields. Even with this help, sometimes the new plants do not grow well, or the ecosystem looks di erent after restoration than it did before the disturbance. Why does restoration sometimes not work as well as other times? Recently, ecologists have begun to understand that successful ecosystem restoration needs to take care of both the plants and animals aboveground and the organisms that live belowground, in the soil.

WHY ARE THE SMALL CREATURES LIVING IN THE SOIL SO IMPORTANT?
Because plants cannot move, they use their roots to look for nutrients and water in the soil. The soil is an important part of the ecosystem and is the habitat of many small creatures [ ]. One teaspoon of soil is inhabited by more organisms than there are people on earth-around  water from the soil, therefore helping the plants to grow better. So, let us call them helper fungi from now on. In addition, these helper fungi can protect plants against pathogens that can cause plant diseases! In return for the benefits helper fungi provide, plants give helper fungi some of their carbon-a nutrient that plants produce with the help of sunlight during photosynthesis. So, the partnership between plants and fungi is a win-win situation for both partners, called symbiosis.
Ecologists tested the e ects of the helper fungi for assisting ecosystem restoration ( Figure ). The process of adding helper fungi to the soil is called soil inoculation. Studies testing the e ects of soil inoculation

SOIL INOCULATION
Adding soil organisms (for example, mycorrhizal fungi or bacteria) to the soil to make plants grow better.
on ecosystem restoration showed that replanted tree seedlings and grasses grew better when helper fungi were added to the soil [ ]. In addition, the studies showed that grasslands restored by adding helper fungi were richer in plant species.

THE POWER OF HELPER FUNGI VARIES
Di erent types partnerships exist between helper fungi and plants, depending on which species of fungi are most abundant in a specific ecosystem ( Figure B). Plants and helper fungi di er in when and how much they need each other and how fairly they trade resources and benefits with each other. So, restoration of ecosystems with plants that depend strongly on helper fungi might need mycorrhizal fungi as a medicine more than other ecosystems in which the plants are more independent from helper fungi [ ]. When scientists looked through all ecosystem restoration experiments that used helper fungi, they actually did find that the benefits of adding helper fungi varied between studies [ ]. So, helper fungi are a good medicine for plants, but when they are most powerful depends on the plants and the ecosystem.
The scientists concluded that helper fungi were the most helpful in the following three conditions: • for plants that give shelter to bacteria in their roots, making the plants hungry enough for two-themselves and the bacteria (like pea or bean plants); • for plants that have trouble finding nutrients because their roots are too big to reach into the small soil pores where many nutrients are sitting (like some grasses); and • for plants growing on soils that are both severely disturbed and nutrient-poor.

WANT TO HELP YOUR OWN HOUSE PLANTS?
About % of all plants-even your potted plants at home-have a symbiosis with one type of helper fungi, called arbuscular mycorrhizal fungi. You can keep your plants healthy by making your own arbuscular mycorrhizal fungal inoculum (Figure ). Many gardeners already do this! You will need a shovel, a -liter bucket to mix the soil, -l of sand, seeds of grass and clover plants, and -flowerpots.
The steps: • Plant selection: Select one or two plants from your garden that form arbuscular mycorrhizal symbioses (maybe a grass and a clover plant) and dig them out together with a good portion of soil around the roots.
• Source inoculum: Remove the leaves and stems of both plants, cut their roots into small pieces, and mix all roots and soil well. This is your source inoculum, which we will multiply so that we do not have to dig out too many garden plants to create the inoculum. • Inoculum multiplication: Mix the source inoculum with sand ( part of inoculum with part of sand, or even of part inoculum with parts of sand) and put it into the flowerpots. Add the grass and clover seeds to the pots, water them, and let them grow for -months. The seeded species are called bait plants

BAIT PLANTS
Plants used as a lure to attract mycorrhizal fungi in their roots, where mycorrhizal fungi reproduce and grow. The multiplied fungal material in the roots and the surrounding soil can then be used to produce a fungal inoculum. because they attract the arbuscular mycorrhizal fungi. When the bait plants start growing, the arbuscular mycorrhizal fungi will colonize their roots and the fungal population will grow. The longer the plants grow, the more fungi will be in the soil. • Inoculum harvest: After -months, remove all leaves from the bait plants, cut their roots into small pieces, and mix them with the soil. The inoculum is now ready to be added in small amounts to your potted plants-either dry or mixed with water.

AUTHOR CONTRIBUTIONS
LN and NM conceived the original idea and wrote the first draft of the manuscript. All authors gave final approval for submission. . doi: . /frym. .

CONFLICT OF INTEREST:
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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