Can Methane-Eating Bacteria in Drylands Help Us Reduce Greenhouse Gases?

What is a dryland? The first thing that may come to your mind is a desert-like place where nothing can live or grow. Despite the scarcity of water, dryland ecosystems are diverse and will expand due to global climate change. The main cause of global warming is the increase of greenhouse gases in our atmosphere. To solve this, we obviously need to reduce the emission of greenhouse gases, but the study of microorganisms in nature also gives us exciting clues for how to address the problem of global warming. Microorganisms live in all possible Earth environments, and luckily some of them can even take greenhouse gases from the air as their food! In this article, we describe our search of the global soils for bacteria that can consume one of the most powerful greenhouse gases, methane (CH4). Contrary to what was expected, we found that these bacteria live in drylands all over the world!


Figure Figure
Methods that we follow to find and study soil methanotrophs. We selected drylands all around the world and took soil samples ( ). We analyzed the properties of those soils, such as organic matter content and pH ( ). We extracted the genetic information (DNA) of the bacteria present in the soil ( ). Studying the DNA, we obtained information about the abundance, richness, and community structure of the methanotrophs from each soil sample ( , ). Then we used mathematics to figure out which are the most relevant soil or climate conditions for methanotrophs ( ).

THE LARGEST TERRESTRIAL ECOSYSTEM ON EARTH: DRYLANDS
Drylands are characterized by scarce rainfalls and consequently do not have lush vegetation. However, drylands cover a whole range of di erent ecosystems, from the driest place on Earth, the hot desert of Atacama in Chile, to the leafy eucalyptus forests in Australia where Koalas live ( Figure . ). Dryland ecosystems also contain huge numbers of organisms, many of which are plants and animals that live only in drylands and have adapted to the harsh conditions. Drylands are the biggest terrestrial ecosystem, occupying almost half of the Earth's land surface ( %) and are home to more than % of the human population. So, you can see why drylands are extremely important areas of the earth to research.
Living beings and non-living substances in the environment, like plants and water, are intimately connected in by cycles in nature. These non-living substances are called abiotic factors. Water is ABIOTIC Non-living abiotic factors in an environment include temperature, water, and light. crucial for all processes that are related to life, from plant growth to the development of communities of soil microorganisms. Therefore, water is the most important abiotic factor in an ecosystem. We measure the availability of water in an ecosystem with a measurement called aridity, a mathematical relationship between the amount of ARIDITY Mathematical relationship between the amount of precipitation (rain, fog, or snow) and the evaporation of water. It describes how deficient water is in an ecosystem. precipitation (rain, fog, or snow) and the evaporation of water. The less available water there, the more arid a place is ( Figure . ).
In drylands, where water is not always available, the natural cycles between living beings and non-living substances are hugely a ected.
When there is no water from rain and the humidity goes down, this a ects the carbon (C) and nitrogen (N) cycles, reducing the abundance of these elements in soil, which impacts plants, animals, ABUNDANCE The number of individuals of a certain type present in an environment. and microorganisms. All this makes drylands extremely vulnerable to ongoing climate change.

SOIL BACTERIA AND METHANE
The Earth is surrounded by a gaseous layer called the atmosphere, which protects us form solar radiation and helps maintain the overall temperature of the Earth. The principal components of the atmosphere are nitrogen ( %) and oxygen ( %), but there are many other gases in the atmosphere as well. Some atmospheric gases, such as carbon dioxide (CO ) and water vapor, are the greenhouse gases, so called because they trap the Sun's heat, working like the glass in a greenhouse. The greenhouse gases let the Sun's light reach the Earth's surface but prevent heat from leaving the atmosphere. This trapping of heat contributes to global warming.
The most abundant human-produced greenhouse gas in the atmosphere is CO , released from burning fossil fuels. However, the second most important gas contributing to global warming is methane (CH ). Methane is a simple molecule formed by one atom of carbon (C) and four atoms of hydrogen (H). The warming e ect of one molecule of methane is equivalent to molecules of CO , making it a super-powerful greenhouse gas. Methane is produced by methanogens, a group of microorganisms that do not need oxygen METHANOGENS Group of microorganisms that do not need oxygen to survive and therefore can live in oxygen-free environments. They produce methane while decomposing organic matter, such as leaves or wood fragments. to survive and therefore can live in oxygen-free environments like rice fields, lake sediments, and wetlands. Methanogens also live in the digestive tracts of animals, such as the stomachs of cattle and even in humans! Methanogens are responsible for animal burps and farts! Methanogens also produce methane while decomposing organic matter, such as leaves or wood fragments. In addition to agriculture, other human activities, such as the oil and gas industries also release large amounts of methane into our atmosphere [ ] (Figure ).
The methane released into the atmosphere is greatly contributing to climate change and there is only one group of organisms that can consume it, the methanotrophs. This group of microorganisms METHANOTROPHS Group of microorganisms that are capable of using up methane as their source of carbon and energy. They are methane eaters. is capable of using up methane as their source of carbon and energy. These microorganisms basically eat methane ( Figure )! In drylands, methane production is low because of the scarcity of water (remember, methanogens typically live in flooded soils and other oxygen-free environments). However, due to the large extent of drylands and the global increase of methane in the atmosphere, dryland ecosystems could be of great interest if methanotrophs are also present and abundant there.

HOW TO FIND AND STUDY METHANOTROPHS
In our research, we were interested in learning if methanotrophs are common in the soils of drylands worldwide, and if they are sensitive to climate conditions and soil properties, like most soil microorganisms. First, we selected dryland sites all around the world ( Figure . ). At each site, we collected climate information, such as mean annual temperature, annual precipitation, and aridity. We also took soil samples and analyzed properties, such as the amount of organic matter (Organic Carbon), pH, and sand content ( Figure . ). High soil organic matter indicates the soil is fertile, meaning it has the nutrients that plants, soil animals and microorganisms need to grow. pH analysis tells us how acidic the soil is. pH is one of the most important factors regulating soil bacteria growth. For example, when soils are very acidic like vinegar, only specific acid-tolerant bacteria can live there. Soil grains are very close to each other but also leave spaces for air and water to enter. The amount of sand, the largest type of particle in the soil, tells us how big these soil spaces are. This way, a high sand content means there are big spaces, so that air can enter the soils easily, but water and nutrients can also drain easily.
To study the methanotrophs in our soil samples, we need the genetic information (DNA) of these bacteria [ ]. First, we obtain all the DNA present in our soil samples, through a process called DNA extraction DNA EXTRACTION Laboratory procedure in which cells are broken open to release the genetic material (DNA) they contain inside, without damaging the DNA.
( Figure . ). This process is done in the laboratory using powerful enzymes that break the cells open without damaging the genetic information. We then analyse the extracted DNA for a specific region that is present only in methanotrophs. This stretch of DNA is a gene called pmoA. The pmoA gene contains the instructions for the protein that allows methanotrophs to eat atmospheric methane. Knowing the concentration of the pmoA gene in each soil sample tells us how many methanotrophs were living in that sample ( Figure . ). There are several closely related species of methanotrophs that all have similar DNA information, but di erent species have tiny genetic di erences in kids.frontiersin.org June | Volume | Article | Figure   Figure Microbial communities can be described using three properties. Abundance is the total number of bacteria of a certain type present. Richness is the number of di erent kinds of bacteria present in an environment. Community structure describes how many di erent kinds of bacteria there are and how abundant each kind is.
their DNA, so we can use DNA to identify di erent methanotrophs, like a fingerprint ( Figure . ).
Our DNA studies help us to obtain information about the abundance (total number of bacteria of a certain type present), richness (the

RICHNESS
The number of species (di erent kinds) of organisms present in an environment.
number of di erent kinds of bacteria present), and community structure (the di erent kinds of bacteria and the abundance of each

COMMUNITY STRUCTURE
The combined richness and abundance in the community.
kind) of the methanotrophs from each soil sample (Figure ). Then, we use mathematics to figure out which soil or climate conditions are the most important for methanotrophs ( Figure . ).

WHERE THE METHANOTROPHS LIVE
We were not sure if we would find methanotrophs in drylands, since these microorganisms need methane to live and drylands are not the typical ecosystem for methane production. So, finding methanotrophs in all our dryland soil samples was an extraordinary finding! We can now say that methanotrophs are widely distributed in global drylands. Surprisingly, we even found some methanotrophs that are usually found in humid places, such as Denmark, Scotland, or New Zealand.
We also found that, in drylands, the average annual temperature and aridity are not the main conditions influencing the abundance and richness of methanotrophs. Abundance and richness may be driven by other factors, such as rainfall. However, climate conditions like mean annual temperature, rainfall, aridity, and soil properties, such as organic matter, pH, and sand content did a ect the community structure of methanotrophs. For example, higher temperatures increased the abundance of certain methanotrophs that are heat-resistant. In other words, those drylands with higher temperatures, the methanotroph communities may contain more heat-resistant methanotrophs. Climate conditions can also a ect soil properties, for example by favoring the breakdown of rocks, which increases sand content, or by modifying soil pH and organic matter. These soil properties a ect the amount of air that can get into the soil, which we found to be very important for the community structure of the methanotrophs.

WHAT WE LEARNT FROM METHANOTROPHS IN DRYLANDS?
As we found, methanotrophs are abundant and widely distributed in drylands worldwide. Both climate and soil a ect the communities of methanotrophs. Moreover, we found the community structure of methane eating bacteria was highly dependent on climate conditions, such as amount of rainfall and temperature, and soil characteristics as soil organic content.
Because we found that climate influences the methanotrophs, we expect that ongoing climate change will modify methanotroph communities in the coming years, a ecting the consumption of atmospheric methane. To date we knew methanotrophs lived on cold and humid places, which will be surely a ected by climate change. The vast amount of land that drylands cover and the many methanotrophs they contain may make these areas extremely important for consuming atmospheric methane in the future. In other words, dryland bacteria can help us reduce greenhouse gases! Taking good care of drylands now and continue studying the hidden marvels they contain is important to deal with our future warmer planet. Methane-Eating Bacteria in Drylands can help us!
. What can DNA in the environment tell us about an ecosystem? Front. Young Minds : . doi: . /frym. .