Potential Use of Biological Herbicides in a Circular Economy Context: A Sustainable Approach

In recent years, synthetic herbicides' intense and disordered use has triggered severe contamination of soils and water bodies, causing damage to living organisms, including vegetal herbicide-resistance. In-part, biological herbicides, offer promise in addressing these problems, especially for sustainable weed management in both agricultural and natural resource management contexts. The search for culture media to serve as alternatives to the existing conventional synthetic media has focused on microalgae biomass. The natural properties of these organisms make them ideal raw materials for fermentative processes. Therefore, this study aimed to integrate bioprocesses producing bioherbicidal extract using a submerged fermentation process. For this, a substrate of microalgae recycled biomass to replace commercially used media for fungal growth for relatively cheap biomass obtained from wastewater phycoremediation after anaerobic treatment (UASB). We also verified its potential for weed control. Preliminary tests of microalgae fermentation were conducted in the raw and lyophilized form, using the fungus Trichoderma koningiopsis. To evaluate their phytotoxic potential, the biocompounds were applied to Bidens pilosa, Conyza bonariensis, Urochloa plantaginea, and Euphorbia heterophylla weeds. A central composite rotatable design (CCRD) was used to evaluate the influence of temperature (20–45°C), agitation (0–200 RPM), and pH (3–14) on the improvement of enzyme activities, fungal growth and the effects on Cucumis sativus germination tests. We found that microalgae could be used as a substrate for obtaining bioherbicides. The biocompounds produced in this study were relevant for pre- and post-emergence control of model plants (Cucumis sativus), with vigor indexes of up to 8.05% and herbicide-resistant plants (Conyza bonariensis) with phytotoxic damage of up to 100% after 15 days. From the experiments' design, we found that agitation was the variable that had the most significant effect on enzyme and biomass production, followed by pH and temperature. The enzymes also showed synergistic action when acting together with microalgae and fungi, potentiating the control effect on weeds. Finally, the system integrated bioprocesses through agro-industrial effluent recovery and reuse, considered one of the essential economic viability mechanisms by enhancing the development of more sustainable technologies that embrace the principles of a circular economy.

In recent years, synthetic herbicides' intense and disordered use has triggered severe contamination of soils and water bodies, causing damage to living organisms, including vegetal herbicide-resistance. In-part, biological herbicides, offer promise in addressing these problems, especially for sustainable weed management in both agricultural and natural resource management contexts. The search for culture media to serve as alternatives to the existing conventional synthetic media has focused on microalgae biomass. The natural properties of these organisms make them ideal raw materials for fermentative processes. Therefore, this study aimed to integrate bioprocesses producing bioherbicidal extract using a submerged fermentation process. For this, a substrate of microalgae recycled biomass to replace commercially used media for fungal growth for relatively cheap biomass obtained from wastewater phycoremediation after anaerobic treatment (UASB). We also verified its potential for weed control. Preliminary tests of microalgae fermentation were conducted in the raw and lyophilized form, using the fungus Trichoderma koningiopsis. To evaluate their phytotoxic potential, the biocompounds were applied to Bidens pilosa, Conyza bonariensis, Urochloa plantaginea, and Euphorbia heterophylla weeds. A central composite rotatable design (CCRD) was used to evaluate the influence of temperature (20-45 • C), agitation (0-200 RPM), and pH (3-14) on the improvement of enzyme activities, fungal growth and the effects on Cucumis sativus germination tests. We found that microalgae could be used as a substrate for obtaining bioherbicides. The biocompounds produced in this study were relevant for pre-and post-emergence control of model plants (Cucumis sativus), with vigor indexes of up to 8.05% and herbicide-resistant plants (Conyza bonariensis) with phytotoxic damage of up to 100% after 15 days. From the experiments' design, we found that agitation was the variable that had the most significant effect on enzyme and biomass production, followed by pH and temperature. The enzymes also showed synergistic action when acting together with microalgae and fungi, potentiating the control effect on weeds. Finally, the system integrated bioprocesses through agro-industrial effluent recovery and reuse, considered one of the essential economic viability mechanisms by enhancing the development of more sustainable technologies that embrace the principles of a circular economy.
Keywords: bioherbicide, sustainable weed management, residual biomass valuation, circular economy, design of experiments GRAPHICAL ABSTRACT | HIGHLIGHTS -Bioherbicides as alternatives for weed management; -Valuation of microalgae biomass substitutes for synthetic fermentative medium; -Bioherbicides produced by fungus and microalgae selectively control Conyza bonariensis; -Enzymes and bioherbicides have synergistic action in weed control.

INTRODUCTION
One of the narrowest bottlenecks of modern agriculture, preventing productivity, is the presence of hardy weeds. Natural ecosystem dynamics may drive this phenomenon; however, herbicides used to control these plants bear substantial responsibility for generating resistance (Perotti et al., 2020). They can be found in all parts of landscape and property, including rural, urban, coastal, national parks, and private farms, forming large dense patches threatening the ecosystem (Department of Environment Conservation, 2006). The presence of weeds in agricultural fields can generate annual crop losses of more than the U.S. $100 billion worldwide (Appleby et al., 2000). The global consumption of herbicides for managing these weeds is about 95 million tons per year, representing the share of 47.5% among all pesticides used in the world (De et al., 2014). The strong dependence on the intensive and disordered synthetic herbicides for weed control has been questioned for many years. The problems resulting from their overuse have been increasingly frequent and significant. As a result, the public's concern with synthetic herbicides' environmental safety has increased interest in developing alternative methods that are efficient in controlling weeds and minimizing ecological impacts (Cordeau et al., 2016).
A more sustainable alternative for weed management is bioherbicides produced through secondary fungal and plant metabolites. These bioproducts are emerging in the weed control area because they provide a more sustainable pathway for modern agriculture (Cordeau et al., 2016;Todero et al., 2018). The use of microorganisms in agriculture can promote or reduce invasive plants' development as a form of biocontrol and to protect the environment (Boyette and Hoagland, 2015;Radhakrishnan et al., 2018). The restructuring of biodiversity in terms of richness, diversity, and uniformity after applying bioherbicides was more significant than synthetic herbicides (Zhang et al., 2019).
In many countries, bioherbicides are an emerging technology for biological control. There are few bioherbicides on the market, and most are aimed at the organic market and have few or no technical details on application costs. Thus, it is a great challenge to obtain this information since this content is not available in the public domain, either through published patents or scientific publications (Mupondwa et al., 2015). So far, more than 20 bioherbicides have been officially registered worldwide (Zhang et al., 2019). Some of which have already had their sales interrupted due to production and marketing costs. Others are still in the testing phase to overcome commercialization challenges and competition with cheaper synthetic herbicides (Cordeau et al., 2016).
To date, few studies provide a complete techno-economic evaluation in studies involving the production of bioherbicides by the microbial route. More than 50% of the operating costs for formulating a bioherbicide are associated with raw material acquisition when commercial and chemically defined (Mupondwa et al., 2015). Microalgae biomass in fermentation processes is a relatively recent issue. Some microalgae can be grown in residues from the production of biogas, the digested ones, to recycle nutrients such as phosphorus and nitrogen present in this material. In biotechnological processes, residual biomass presents potential as fermentative substrates. It is a cheap source of raw material and rich in proteins and carbohydrates. Thus, the reintegration of this biomass in new processes allows the development of the circular economy, adding value to its use, bringing economic viability through the use of cheap and environmental biomass for the proper management of residues from biogas production (Michelon et al., 2016;Stiles et al., 2018;Drumm et al., 2019).
Recycled microalgae biomass allows simultaneous organic waste management and supplementation through its use as a substrate to improve fermentation performance and end-product efficiency. The application of these microalgae from digestate management provides a circular economy developmental approach, enabling alternative uses of the same biomass and establishing promising solutions in food and environment security (Stiles et al., 2018). Recycled microalgae biomass is promising in biotechnological processes, as are bioherbicides because they are strategies that aim to minimize the exploitation of natural resources and costs. They seek the insertion of more natural and eco-friendly products into the global market. Therefore, the study aimed to integrate bioprocesses producing bioherbicidal extract with a submerged fermentation process using recycled microalgae biomass as a substrate. The aim was to replace commercially-available media for fungal growth for relatively cheap biomass obtained from wastewater phycoremediation after anaerobic treatment (UASB) and to verify its potential in the control of weeds resistant to synthetic herbicides. The circular economy integration with an approach in bioherbicide production remains a new subject in the literature. To the best of our knowledge, this is the first study focused on that process that seeks to produce a closed cycle in the entire swine production chain, biogas energy generation, and recovery of raw material, resulting from the polishing of effluents for microorganism cultivation, aiming for the production of bioherbicides.

Microalgae Biomass and Fungal
The microalgae used in this study derive from Chlorella spp. obtained from phycoremediation of swine wastewater, implemented at EMBRAPA Swine and Poultry (Concórdia, SC, Brazil). Microalgal biomass comprises 56.1% protein, 34.7% carbohydrate, 1.7% lipid, and 7.8% minerals. To produce the biocompound from microalgal biomass, we used the fungus Trichoderma koningiopsis, a microorganism isolated from Digitaria ciliares (Reichert Júnior et al., 2019). The microorganism's growth and maintenance occurred in Potato Dextrose Agar (PDA) and was maintained in a bacteriological greenhouse at 28 • C for 7 days before the start of fermentation.

Fermentation Process
Preliminary tests were performed relating the biochemical composition of microalgae to the chemical composition of synthetic media optimized by Bordin et al. (2018), using microalgal biomass under different conditions: (1) raw biomass (with 89% humidity); (2) lyophilized biomass (72 h; 50 • C and vacuum pressure); (3) raw and lyophilized biomass pretreated with an ultrasound probe (80% power, 10 min time and three pulses). Table 1 describes the composition and conditions used in the preliminary tests.
The fermentation medium was carried out in 300 ml flasks with a useful volume of 150 ml and sterilized in an autoclave for 20 min and 121 • C. After T. koningiopsis was inoculated with

Assay
Microalgae Description    approximately 10 6 spores m L −1 . Fermentation was maintained on an orbital shaker at 28 • C, 120 RPM for 72 h. The final biomass (containing microalgae plus fungal mycelium) was determined using the dry cell mass method, expressed as grams of dry biomass per liter (Gern et al., 2008), and the liquid extract (filtered, named by this study as biocompound) was used for enzymatic quantification.

Enzymatic Activities Quantification
The quantification of amylase enzymes was based on the adapted methodologies of Fuwa (1954) and Pongsawadi and Yagisawa (1987). For cellulases, the filter paper was used as a hydrolysis substrate Ghose (1987). After enzymatic reactions, the release of reducing sugars was measured by the DNS (3,5 dinitrosalisylic acid) method of Miller (1959).
Where: (Aa) mean of samples; (Vb) control value; (MNaOH) molarity of sodium hydroxide; (t) reaction time; (Vc) volume of extract used. Peroxidases were quantified using the method proposed by Khan and Robinson (1994) and Devaiah and Shetty (2009). We added 1 mL of the fermented extract to the reaction medium and subjected to the thermostatic bath for 20 min at 35 • C. The final reaction was analyzed using a spectrophotometer at 470 nm.
To determine laccase activity, the methodology proposed by Hou et al. (2004) was adapted using 2,2 ′ -azino-di-(3ethylbenzothialozin-6-sulfonic) (ABTS) as the enzyme substrate. The reaction mixture contained 0.4 mL of ABTS 10 mM and 3.4 mL of sodium acetate buffer 50 mM, pH 4.8, and 0.2 mL of the enzyme extract and was subject to a thermostatic bath at 40 • C for 5 min. The samples were analyzed using a spectrophotometer at 420 nm.

Resistant Weed Leaf Surfaces
The fermented biocompounds in the preliminary tests were evaluated for their bioherbicidal potential against weeds grown in a greenhouse. Leaf damage was evaluated after 7 and 15 days of treatment based on methodologies recommended by the Brazilian Weed Science Society (1995). The biocompound was applied using a brush because of the viscosity of the extract. The plants selected for the study included resistant to commercial herbicides: Bidens pilosa, Conyza bonariensis, Urochloa plantaginea, and Euphorbia heterophylla (Heap, 1999(Heap, , 2019Cerdeira et al., 2007;Dias et al., 2018).

Germination Tests
The activity of biocompounds was evaluated in the germination of seeds of the species Cucumis sativus. They were performed in previously disinfected Gerbox boxes and lined with Germitest R moistened with the respective treatment (biocompound produced), according to the Rules for Seed Analysis (Brazil, 2009). Four replications of 25 seeds were sown, totaling 100 seeds per treatment, conditioned in BOD, at a constant temperature (25 • C), and a photoperiod of 12/12 h of light/dark.
We analyzed the number of germinated seeds and the number of seeds that germinated and became healthy plants for the germination test. The seeds were evaluated at 4, 8, and 10 days after the treatment, and we measured the hypocotyl and primary root length (plants with hypogeal germination). Germinated seeds are those grown to the top radicle of 2 mm. Germination and vigor indices were calculated using Equations 2 and 3, respectively.
Where, (ni) is the number of seeds germinated in each repetition and (A) is total seeds used.

Statistical Analysis
Analysis of variance (ANOVA) and the effect of variables were used to validate data using the tool Protimiza Experimental Design (Rodrigues and Iemma, 2014).

Enzyme Production and Substrate Interaction
From the initial fermentation tests, we found that there was substantial production of peroxidases using lyophilized biomass. The ultrasonic pretreatment was not determinant for the production of this enzyme ( Table 2). In comparison with the results found by Bordin et al. (2018), using a synthetic medium for the growth of the fungus, greater production of peroxidases is observed using microalgae biomass. For the other enzymes, the replacement of the synthetic medium by biomass was not promising. Assay L4 was the largest producer of peroxidase and amylase (16.200 and 6.98 U mL −1 , respectively). The assay L3 presented the highest cellulase and biomass production. Cellulose is the main carbohydrate in microalgae's cell wall and is prone to enzymatic degradation, whereas starch, the substrate of amylase, is found in plastids (intracellular) (Chu et al., 1982;Sander and Murthy, 2009;Velazquez-Lucio et al., 2018). The high biomass production may be linked to the higher substrate existence (66.6 g L −1 ) and also to the higher cellulase yields that break down the cellulose and generate a valuable substrate for fungal growth, the glucose.
Studies that analyzed the co-cultivation of microalgae with filamentous fungi in pelletizing processes, to remove microalgal cells from the liquid medium, found that microalgae cells were incorporated into the fungal hyphae mesh, with increased biomass production without a change in the composition of the microalgae lipids. The synergistic effect of biomass production and lipid accumulation is also due to the fungal utilization of carbohydrates in the microalgae cell wall (Xie et al., 2013).

Application on Resistance Weeds
The biocompounds obtained in the F4 and L4 assays showed better enzymatic responses, so they were applied to weeds to evaluate the phytotoxic effects through the biocompound, produced using the raw and lyophilized biomass. Table 3 presents the results of the phytotoxic damage found and compared with other studies in the literature. The percentage of effects were related to the control assay, which used only distilled water.
There was a selective effect on the weed Conyza bonariensis. That is a 100% phytotoxic effect within 15 days after applying the extract using raw microalgae biomass and fungus (Figure 1). This species can produce an excessive number of viable non-dormant seeds that can germinate and complete various life cycles in 1 FIGURE 1 | Illustration after the 15th day of treatment with the biocompound produced.
year, suggesting that their control is extremely important (Wu et al., 2007).
The phytotoxic effects after the 15th day in C. bonariensis were greater with the use of biocompound produced from raw biomass in fermentation than lyophilized biomass. In addition to reducing costs with the microalgae drying process, these results are interesting because they suggest satisfactory control of a species that is considered highly resistant to conventional herbicides. Studies such as Hosseinizand et al. (2017) revealed that microalgae drying processes could produce adverse effects on Chlorella surface lipids' color, structure, and composition, and carbohydrates. In this case, these adverse effects may be influencing the excretion of enzymes and other bioactive metabolites in fermentation with T. koningiopsis and consequently altering the biocontrol characteristics in C. bonariensis between the two biocomposites. Enzyme action has been described as a key mechanism to allow the entry of microorganisms and their compounds into the target plant, facilitating the access and dispersion of pathogens through the host (Reichert Júnior et al., 2019). They may be associated with virulence factors as they act in the degradation of the walls of plant cells, proteins and, lipid membranes (Cordeau et al., 2016). Several microorganisms have enzymes, and other bioactive secondary metabolites, that can combat or prevent chemical defense mechanisms of plants and regulate plant growth (Keen and Staskawicz, 1988;Vinale et al., 2008). Some strains of Trichoderma spp. are prolific enzyme producers, including those that degrade plant cell walls (Harman et al., 2004;Noll et al., 2016;Seppälä et al., 2017). The enzymes quantified in this study have been reported to be auxiliary agents attenuating of phytotoxic effects on plants during biological control because of their possible mechanisms of action on plants (Cordeau et al., 2016;Bordin et al., 2018;Reichert Júnior et al., 2019).
The effects of selectivity for Conyza bonariensis are related to possible compounds released by microalgae during enzymatic degradation. In the fermentation process, because the results of phytotoxicity in studies of T. koningiopsis for growth in commercial media (glucose, peptone, yeast extract, and other salts) were specific for the species Euphorbia heterophylla (Bordin et al., 2018;Reichert Júnior et al., 2019). Recently Coelho et al. (2019) found that the degradation of Chlorella vulgaris cell walls from an enzymatic pool generated the release of bioactive compounds such as proteins, saturated and monounsaturated fatty acids, amino acids and some types of carotenoids such as β-carotene, astaxanthin, canthaxanthin, and lutein, all of which have antioxidant activity (Gouveia et al., 2006). It was speculated that these compounds, along with the fungus metabolites, may exhibit certain types of selectivity as biocontrol agents in plants; however, the mechanism by which this occurs remains unclear.
In comparison with other studies presented in Table 3, promising results using the fungus T. koningiopsis were also found by Camargo et al. (2019) in B. pilosa, C. bonariensis, and E. heterophylla with up to 100% phytotoxicity. In the study, the authors presented an innovative solution combining synthetic herbicides (2,4-dichlorophenoxyacetic acid, glyphosate, and ammonium glufosinate) in fermentation with T. koningiopsis generating an effective compound with synergistic action between bioherbicides and synthetic herbicides in the control of the three plants. In the studies by Reichert Júnior et al. (2019), more promising results were found with the use of adjuvants and with a repeated application on target plants up to 8 times within 30 days. Some studies show that when exposed to stress conditions, plants produce a large number of reactive oxygen species (ROS). These substances oxidize and modify cellular components such as proteins, lipids, and nucleic acids, preventing them from performing their original functions. They are highly harmful at high concentrations. ROS regulates stomatal behavior, pathogen defenses, and various processes, including programmed cell death (Apel and Hirt, 2004;Mittler et al., 2004;Gill and Tuteja, 2010;Hoseini and Zargari, 2013;Schippers et al., 2016;Huang et al., 2019). One of the mechanisms used by plants to circumvent such a situation is based on enzymatic antioxidant defense systems (catalase, peroxidase, superoxide dismutase, glutathione reductase, etc.), and non-enzymatic systems that act synergistically and interactively to neutralize free radicals (Apel and Hirt, 2004;Benavides et al., 2005;Huang et al., 2019). When there is an imbalance in cells between elimination systems and ROS levels, the cells enter an oxidative state that can lead to death (Huang et al., 2019). High concentrations of the peroxidase enzyme in the biocompound formulation may cause stress symptoms in the plant because these enzymes act on the polysaccharide cell wall structure. The high percentage of phytotoxic effects in C. bonariensis suggest that there are increased levels of ROS interacting with the plant defense system, resulting in oxidative modification. It is also interesting to mention that Trichoderma has already been disclosed as a microorganism that increases defense-related plant enzymes production, including various peroxidases, chitinases, β-1,3glucanases as well as the lipoxygenase hydroperoxide-lyase pathway (Koike et al., 2001;Howell, 2003;Vinale et al., 2012). These findings, combined with the possible antioxidant products released by microalgae during fermentation, could explain the phytotoxic effects presented and the selectivity for C. bonariensis species.

Analysis of Design of Experimental Responses
The analysis of enzyme quantification during the biocompound production process is presented in numerous studies that relate the phytotoxic potential through the action of extracellular enzymes such as cellulases, peroxidases, lipases, amylases, etc. (Bordin et al., 2018;Camargo et al., 2019;Reichert Júnior et al., 2019). The conditions studied in the planning aim to explore enzymes' production resulting from the interaction between T. koningiopsis and microalgae biomass during the fermentation process. The investigated variables can modify the behavior of the microorganism in this unconventional substrate.One of the limitations to the industrial implementation of microorganisms isolated from the environment, as in T. koningiopsis, is that the product spectrum generally varies with thermodynamic expectations, even if the system is systematic and repeatable (Temudo et al., 2007). This limitation occurs mainly due to a lack of understanding of how operational factors affect the microbial community/functionality and, therefore, the product spectrum (Mohd-Zaki et al., 2016).
Previous studies showed that changes in fungal morphology could be influenced by medium composition, pH, culture temperature, agitation, aeration, among others (Papagianni, 2004;Liao et al., 2007). Each factor mentioned has different importance for growth morphologies. For example, some strains of Rhizopus sp. require strong agitation, while strains such as Penicillium chrysogenum require high pH values (Metz and Kossen, 1997).
With the satisfactory results obtained using gross microalgal biomass in the initial tests, we decided to continue with this type of biomass in the experimental design. Because of the high production of peroxidases in the preliminary tests, we decided to analyze laccases activities. Table 4 presents the planning matrix and the answers obtained.
Trichoderma species such as T. harzianum have already demonstrated laccase production potential; temperature and pH effects during microorganism growth were important for enzyme production (Ranimol et al., 2018). The authors' maximum activity was 0.971 U mL-1 after 12 days of incubation at pH 5.5 and 30 • C (Ranimol et al., 2018). Other studies reported that laccase activities were positive when there was an additional nitrogen source in the medium. Peptone and casein, two organic nitrogen sources, showed better laccase inducers than inorganic nitrogen sources (Mikiashvili et al., 2006;Bettin et al., 2009;Schneider et al., 2018). In the present study, no external nitrogen source was added, possibly explaining the low laccase yields. Nevertheless, the results obtained after 72 h of incubation with T. koningiopsis in microalgae biomass are promising compared to those found by Ranimol et al. (2018). The best activities were 12 days of fermentation under synthetic media.

Statistical Analysis
Using the Protimiza Experimental Design tool, the possible effects of the studied variables were analyzed, and their interaction in determining enzymatic activities and biomass production. Figure 2 presents the Pareto charts, with a statistical confidence of 95%.
Amylase had a positive and significant effect on agitation (p < 0.05). Table 3 displays the maximum amylase production in the highest agitation assays (200 RPM), suggesting that strong agitation facilitates starch availability inside the microalgal cell, as a substrate for amylase. The effect of agitation was also significant and positive for cellulase and peroxidase activities. However, it was significant and negative for biomass production. These results may suggest that high agitation produces a stress condition to the fungus, releasing enzymes into the liquid medium. The medium agitation influences the free enzymes in contact with the bioavailable substrate; they tend to favor the enzyme-substrate interaction mechanism, producing desirable effects for enzymatic production. Nevertheless, for laccase and lipase, no physical parameters were statistically significant, suggesting that a deeper analysis on the interaction of the filamentous fungi with the microalgae in fermentation processes should be undertaken, since it was already seen that during the interaction there was no lipid consumption (Xie et al., 2013).
Using analysis of variance, we found that only the cellulase response had its model validated with 95% confidence; F 4 | Matrix of experimental design central composite rotatable design (CCRD) 2 3 (coded and real values) and respective answers in terms of enzymatic and biomass production.

Germination Analysis in Cucumis sativus
The biocompounds produced in the experimental planning were applied and analyzed against seed pre-germination (Table 5).
Generally, Cucumber species are used as model plants because they are susceptible and are considered good bioindicators (Redda et al., 2018;Todero et al., 2018). Table 5, together with Figure 3, shows the results found 8 days after the application the biocompound in the C. sativus seeds.
The bioherbicidal effect has been demonstrated for some of the biocompounds evaluated in C. sativus. A particular case was the germination inhibition result for test E14. No hypocotyl and primary root measurements or vigor indices were observed. Non-germination is explained by the high pH (14). A positive control with water at pH 14 was performed, and no germination rate was recorded (Figure 4). Even with promising effects, it is not the objective of this work to generate a biocompound with bioherbicidal effects at pH 14, because in real applications, the use of a bioherbicidal agent at such high pH could have undesirable consequences for other cultures, microorganisms, and even for the environment. The justification for having analyzed such an extreme range was to investigate the microorganisms' performance involved in the fermentation process and its concomitant enzyme production.
Other results of germination rates showed no inhibitory effect on C.sativus. Results lower than 100% of germination are attributed to repetitions between 23 and 24 seeds, germinated in the Gerbox. Thereby generating 98% germination rates for E1 and E5, and 99% for E9 and E10.The main data to be interpreted are represented by the control with seeds soaked only with distilled water, which still did not show the best results, suggesting that some biocompounds can assist in plant development. Nevertheless, we observed changes in some plants' growth that suffered some retardation of their physiological development ( Table 5).
Some treatments showed an effect on the plants' deficiency. Even presenting root and hypocotyl development, they contained fragile physical structures that were very sensitive to any contact. Examples are plants that have been treated with biocompounds E2 (Figure 5A), E11 and E16.
Plants that were in contact with biocompound E11, for example, also presented a reduction in the hypocotyl development (averages of 37.37 mm) ( Figure 5B). The same occurred with plants exposed to the biocompound E16 ( Figure 5C). Also, demonstrating developmental deficiencies and breaking sensitivity had a low hypocotyl length of only 32.56 mm and a 30.03 mm root. Frontiers in Sustainable Food Systems | www.frontiersin.org 9 October 2020 | Volume 4 | Article 521102 Thus, the results presented in Table 5 show that the treatments were efficient for the germination of seeds exposed to the 2.51 mm of E1 biocompound. For the development of hypocotyl and 5.70 mm primary root ( Figure 5D). For the 4.16 mm E9 biocompound for hypocotyl development and 18.26 mm root ( Figure 5E). The E13 biocompound showed no hypocotyl development but roots with 10.90 mm ( Figure 5F). It is also noteworthy that although the E7 biocompound produced adequate hypocotyl, with 58.28 mm. The primary root length of 63.33 mm, its root system, was very thin with a set of deficient and sensitive roots ( Figure 5G). The same happens with biocompound E15 that, in addition to having fragile roots, showed deficiencies in its leaf development ( Figure 5H).
Colonization of Trichoderma spp., in plant roots, caused metabolic changes and pathogenesis-related protein activations. That induced more excellent plant resistance to subsequent attack by numerous microbial pathogens (Vinale et al., 2012).
Although in this study, satisfactory analyses were observed in terms of germination rates of C. sativusseeds (Table 5), we found that all biocompounds affected plant morphology after 8 days of application (Figure 2). The genetic approach and opposition to the physiological investigations carried out in this study could explain the morphological changes that occurred during the plant after germination. This approach could elucidate the enzymes' effects during the development stage since the germination rates and the vigor index consider only physiological parameters. ROS has been found to interact with epigenetic modifiers and hormones that influence various genes' expression and control plant development processes (Gill and Tuteja, 2010;Zeng et al., 2017;Kong et al., 2018).
In this work, we focused on "minimizing costs" in the context of the bioprocess to formulate a biocompound with potential bioherbicide. The biomass of microalgae grown in swine wastewater proved to be a potential source of nutrients. Large-scale production would not be a challenging issue in countries where pig production is abundant. In the fermentative process, biomass can be used in fresh form, without the need for freeze-drying and pre-treatment, thus reducing two unit operations in the process that would directly affect an industrial scale study. It was not necessary to use commercial means and external addition of nutrients for this microorganism. Also, thinking about expanding scale would be a great challenge to overcome as many fungal bioherbicides, seen during this work, use commercial growth media. The investigated parameters of fermentation, such as agitation, temperature, and pH, could be easily adjusted in a bioreactor thinking at an industrial level, without requiring complex investments.

CONCLUSION
Satisfactory biocontrol and selection results were obtained during preemergence for model plants (Cucumis sativus) and C. bonariensis with synergistic effects of secondary metabolites and enzymes produced during the fermentation stage. Responses with up to 100% phytotoxicity were found. The influence of various enzymes can produce stress conditions on the leaf surface of the target plant as well as on the vigor index and morphological characteristics of the seeds. This suggests that the biocompound can be used for pre-and post-emergency control, ensuring greater environmental and food safety. Bioherbicides can be a more suitable and environmentally friendly alternative to commercial herbicides to achieve sustainability in agriculture (Cordeau et al., 2016;Frumi Camargo et al., 2020). Nevertheless, several challenges are overcome, including weed resistance and high production costs with the acquisition of raw material, process factors, the addition of adjuvants, application repetitions, etc. The integration of the circular economy concept in biotechnological processes served in this study as a strategy to minimize the costs in the production process of a biocompound. The use of biomass produced from  industrial processes is a form of polishing effluents from to produce the relatively cheap raw material. The use of these products in fermentation processes benefit the environment, beyond the valorization of raw material.

DATA AVAILABILITY STATEMENT
The datasets generated for this study are available on request to the corresponding author.