Exogenous L-carnitine Administration Ameliorates the Adverse Effects of Heat Stress on Testicular Hemodynamics, Echotexture, and Total Antioxidant Capacity in Rams

Heat stress (HS) diminishes the testicular antioxidant defense systems, which adversely affect the testicular blood perfusion. Improving the testicular hemodynamics during HS conditions is of a great impact on the whole reproductive performance in rams. This study aimed to evaluate the ameliorative effects of L-carnitine (LC) on the testicular blood flow and echotextures and also on the total antioxidants (TAC) and nitric oxide (NO) concentrations in the serum during HS conditions in rams. Testicular blood flow was evaluated through scanning of the supra-testicular artery (STA) spectral patterns through pulsed Doppler ultrasonography [peak systolic velocity (PSV), end-diastolic velocity (EDV), time average maximum velocity (TAMAX), resistive index (RI), and pulsatility index (PI)], while the echotexture assessment of testicular parenchyma was performed by a computerized software program. Moreover, TAC and NO concentrations were assayed colorimetrically using the spectrophotometer. There were significant decreases (P < 0.05) in values of PSV at 48 and 168 h (23.45 ± 0.39 and 23.37 ± 1.41 cm/s, respectively), and TAMAX at 1, 48, and 168 h (17.65 ± 0.95, 17.5 ± 0.13, and 16.9 ± 1.05 cm/s, respectively) after LC administration compared to just before administration (31.92 ± 1.13 and 21.58 ± 0.92 cm/s, respectively). Values of RI and PI of the examined STA significantly decreased, especially at 1 h for RI (0.45 ± 0.02) and 1 and 48 h for PI (0.66 ± 0.06 and 0.65 ± 0.05, respectively) after LC treatment to 0 h (0.55 ± 0.03 and 0.84 ± 0.06, respectively). The EDV values did not show any significant (P < 0.05) changes in all the experimental time points. There were significant (P < 0.05) increases in the values of pixel intensity of the testicular parenchyma, especially at 1 and 168 h (78.71 ± 2.50 and 88.56 ± 4.10, respectively) after LC administration, compared to just before administration (69.40 ± 4.75). Serum NO levels tend to increase after LC administration (P = 0.07) concerning just before administration. While TAC values showed significant gradual increase and reached the highest values at 168 h (2.75 ± 0.58 mM/l) after LC administration, compared to 0 h (1.12 ± 0.05 mM/l). In conclusion, exogenous LC administration ameliorates testicular hemodynamic disruptions, as measured by spectral Doppler ultrasonography, via augmentation of the rams' total antioxidant capacity under HS conditions.

Heat stress (HS) diminishes the testicular antioxidant defense systems, which adversely affect the testicular blood perfusion. Improving the testicular hemodynamics during HS conditions is of a great impact on the whole reproductive performance in rams. This study aimed to evaluate the ameliorative effects of L-carnitine (LC) on the testicular blood flow and echotextures and also on the total antioxidants (TAC) and nitric oxide (NO) concentrations in the serum during HS conditions in rams. Testicular blood flow was evaluated through scanning of the supra-testicular artery (STA) spectral patterns through pulsed Doppler ultrasonography [peak systolic velocity (PSV), end-diastolic velocity (EDV), time average maximum velocity (TAMAX), resistive index (RI), and pulsatility index (PI)], while the echotexture assessment of testicular parenchyma was performed by a computerized software program. Moreover, TAC and NO concentrations were assayed colorimetrically using the spectrophotometer. There were significant decreases (P < 0.05) in values of PSV at 48 and 168 h (23.45 ± 0.39 and 23.37 ± 1.41 cm/s, respectively), and TAMAX at 1, 48, and 168 h (17.65 ± 0.95, 17.5 ± 0.13, and 16.9 ± 1.05 cm/s, respectively) after LC administration compared to just before administration (31.92 ± 1.13 and 21.58 ± 0.92 cm/s, respectively). Values of RI and PI of the examined STA significantly decreased, especially at 1 h for RI (0.45 ± 0.02) and 1 and 48 h for PI (0.66 ± 0.06 and 0.65 ± 0.05, respectively) after LC treatment to 0 h (0.55 ± 0.03 and 0.84 ± 0.06, respectively). The EDV values did not show any significant (P < 0.05) changes in all the experimental time points. There were significant (P < 0.05) increases in the values of pixel intensity of the testicular parenchyma, especially at 1 and 168 h (78.71 ± 2.50 and 88.56 ± 4.10, respectively) after LC administration, compared to just before administration (69. 40 ± 4.75). Serum NO levels tend to increase after LC administration (P = 0.07) concerning just before administration. While TAC values showed significant gradual increase and reached the highest values at 168 h (2.75 ± 0.58 mM/l) after LC administration, compared to 0 h (1.12 ± 0.05 mM/l). In conclusion, exogenous LC INTRODUCTION Global warming is one of the most common topics in the world public opinion these days, with many adverse environmental consequences, especially in the field of animal reproduction competence. Environmental thermal stress (TS) triggers oxidative stress (OS) cascade and deteriorates semen quality and fertilizing potential in rams (1, 2), Holstein bulls (3), Shiba goats (4), rabbit bucks (5), and buffalo bulls (6). In rams, elevated testicular temperature induces OS, which in turn diminishes both sperm motility and viability and increases total sperm abnormalities (7). Thermal stress initiates increases in antioxidant defense systems (8) followed by its decline in long-lasting TS via decrement of the mRNA expression of the mitochondrial superoxide dismutase (SOD)-1 with a subsequent decrease in SOD protein synthesis and activity, and more generation of superoxide anion (SOA) and cell apoptosis (9).
The positive association between arterial blood flow of the testes and its exocrine (sperm) and endocrine (testosterone hormone) functions has been reported in rams (10,11), goat bucks (12), donkeys (13), and stud dogs (14). The main route through which testis could be enriched with oxygen and nutrients supply is the testicular artery (TA). Therefore, any alteration in the testicular blood perfusion affects both spermatogenesis and steroidogenesis, with subsequent loss of fertility (15,16). Spectral Doppler ultrasound is a non-invasive reliable method for an organ function assessment via its blood flow measurement (13,17). The use of testicular blood flow measurement as a potential predictor for male fertility has been applied in many species (14,18,19). Resistant index (RI), pulse index (PI), peak systolic velocity (PSV), end-diastolic velocity (EDV), and timeaveraged maximum velocity (TAMAX) are the most commonly used parameters for providing a clear judgment on the organ hemodynamics (20).
Digital analysis of the testicular tissue B-mode ultrasound image [testicular echotexture (TE)] has become a useful tool for evaluating the male reproductive functions in different animal species (4,21). The pixel matrix of the ultrasound image has a score from 0 to 255, with 0 assessed as black color, and 255 corresponds to white color with varying gray color in-between (21). Testicular histology (22), blood flow (4), and sperm-quality parameters (21) governed the degree of TE.
Decreased testicular blood perfusion in heat-stress conditions was reported in rams (10,11) and goat buck (23). Several attempts have been reported to improve the testicular hemodynamics by administering exogenous treatments in rams and goat bucks with potential outcomes, that finally reflected on the whole male fertility, such as melatonin, gonadotropin-releasing hormone (GnRH), and luteinizing hormone (LH) (12,19,24,25). L-carnitine (LC) is a vitaminlike bioenergetic amino acid that exists ubiquitously in different tissue types, such as heart, skeletal muscle, testes, and epididymis. LC has a substantial role in cell energy production via fatty acids (long-chain) transport through mitochondrial membranes and subsequent ATP synthesis via the β-oxidation process. Moreover, LC clears the mitochondrial matrix from the accumulated toxic acyl-CoA. L-carnitine protects the cell against OS via its antioxidative properties through free radicals (SOA, hydrogen peroxide) capturing and lipid peroxidation inhibition (26)(27)(28). Many studies have declared that LC enhances male fertility via improving reproductive hormones (29,30), semen quality (28), antioxidant capacity (28,31), and gene (GnRH and melatonin) modulation (28,32,33). Therefore, the authors postulated that LC administration to heat-stressed rams could alleviate the heat-stress-mediated testicular hemodynamics impairment and improve testicular functions. To the maximum of the authors' knowledge, studying the effect of LC on the rams' testicular hemodynamics in rams housed under heat-stress conditions has not been elucidated. Therefore, this study was aimed, for the first time, to investigate the effects of LC administration on testicular hemodynamics (RI, PI, EDV, PSV, and TAMAX), TE, serum nitric oxide, and total antioxidant concentrations in heat-stressed Ossimi rams.

MATERIALS AND METHODS
The experimental procedures of this study were carried out in the summer season (June-August 2021) at the farm of the Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza Governorate, Egypt (30.0276 • N, 31.2101 • E), following the accreditation of the ethical committee for animal use.

Animals and Care
Fifteen adult Ossimi rams (55.35 ± 3.5 kg body weight) and aged 3.5 ± 0.16 years, were used in this study. The inclusion criteria were adjusted based on the clinical, cardiovascular, and andrological fitness, based on physical and ultrasound examination. Rams that showed normal heartbeats, pulse rate, capillary refilling time, rectal temperature, and absence of hyperechogenic masses in the testicular ultrasonograms were favored the inclusion criteria. Therefore, only 15 rams were selected, for experimental procedures, out of 23 rams that were submitted to the above-mentioned examinations. Rams' housing was in the open air in a paddock belonging to FIGURE 1 | A schematic diagram shows the experimental design, in which fifteen Ossimi rams exposed to environmental heat stress (temperature-humidity index = 32.5) were administered L-carnitine (LC; 20 mg/kg BW; IV) and underwent ultrasound examination and blood sampling just before L-carnitine administration (0 h) and 1, 4, 24, 48, and 168 h post-LC administration.
our department, subjected to normal environmental light, temperature, and humidity. The feeding regime was a mixed formula of concentrate pellets (14% crude protein and energy requirements of 6.39 MJ/kg diet) and green feed with ad libitum availability of fresh tape water. They were examined regularly during the experimentation with prophylactic vaccination and deworming programs against endemic diseases in Egypt.

Rams' Heat-Stress Assessment
The temperature (T) and relative humidity (RH) at each time point during the study were over 36 • C and 60%, respectively, according to the Egyptian meteorological authority (Cairo, Egypt). The temperature-humidity index (THI) was calculated [THI = T -(0.31 -0.31 RH) (T -14.4)] following a previous study that described the relationship between sheep heatstress and meteorological conditions (34). Based on the study mentioned later, the rams used in this study were subjected to severe environmental heat stress (THI ≥ 32.5).

Experimental Protocol
As explained in Figure 1, heat-stressed rams (n = 15) were examined sonographically either by spectral Doppler for the supra-testicular artery of the right and left testis and Bmode scanning of the testicular parenchyma or blood sampling just before (0 h) treatment with LC. Each ram was treated with a single intravenous (jugular vein) injection of LC [20 mg/kg body weight; Mepaco Corporation, Egypt; (35)] and examined at 1, 4, 24, 48, and 168 h after LC administration. The examination time points were selected based on the LC pharmacokinetics, especially the clearance time (36). All the experimental procedures, namely, testicular hemodynamic measurements and echotextures, nitric oxide assessment, and total antioxidant capacity assay, were repeated three times 2 weeks intervals during the same heat-stress conditions.

Obtaining Blood Samples and Biochemical Analysis
Blood samples (5 ml) were obtained from each ram through jugular venipuncture into plain tubes, just before ultrasonographic assessments at each time point. After centrifugation (15 min) of the drawn blood samples at 1,207 g, the serum samples were harvested and stored (−20 • C) till further measurements of nitric oxide metabolites (NOMs) and total antioxidant (TAC) concentrations.
Concentrations of NOMs were measured using commercial kits (nitric oxide kit, Bio-diagnostic, Dokki, Egypt) using a spectrophotometer at 540 nm wavelength. The NOMs inter-and intra-assay coefficient variations were 6.9 and 5.3%, respectively, with assay sensitivity of 0.225 µmol/l in nitrites form (37).
Assessment of TAC was done using commercial kits (total antioxidant capacity, Bio-diagnostic, Dokki, Egypt) spectrophotometrically at a wavelength of 505 nm, based on a previous study (38). The assay rationale is the measurement of the suppressive ability of the sample on sodium benzoate conversion to thiobarbituric acid substances via reactive oxygen species (ROS) generated from Fenton's reaction (39).

Ultrasound Examination
During all the studied time points, sonographic measurements including pulsed-wave Doppler of the right and left supratesticular arteries (STAs) and TE were carried out by the same investigator. An ultrasound device (SonoScape E1V, SonoScape Medical Corp., China) supplied with a 7-14 MHz linear array probe was used for all ultrasonographic measurements. Following a previous study (23), the animals were restrained properly without any sedative agents administration, and the scrotal wool was shaved totally on both sides of each testis till the spermatic cord, and the transducer was loaded with a large amount of ultrasound gel for obtaining clear images for the testes and STA. Just before the testicular inlet, the rams' STA appeared tortuous and convoluted meshed with testicular veins. The instant spectral pattern of the STA (systole and diastole) governed the vessel's discrimination. After a clear view of the STA waveform, pulsed-wave Doppler was used for measurements of the following parameters: (1) PSV (cm/s); (2) EDV (cm/s); and (3) TAMAX (cm/s). From the later-mentioned Doppler parameters, both RI (RI = PSV -EDV/PSV) and PI (PI = PSV -EDV/average velocity) were calculated. All the device settings (brightness, focus, contrast, and gain) were adjusted and fixed throughout the study by the same investigator. The angle between the targeted vessel longitudinal axis and the Doppler beam was ≤60 • with 0.5 mm Doppler gate diameter and 50 MHz high-pass filters.
For TE assessment (22), a clear image, without artifacts, of the longitudinal and transverse aspects of both testes was frozen and saved for further digital computed analysis. The saved images were recalled and examined for testicular echogenicity (TE; the average number of pixels) and pixel heterogeneity (PH; the standard deviation of the pixels) utilizing image analysis software (Adobe Photoshop 64 CC software; Adobe Systems, 2016), by adjusting a 1 cm × 1 cm square in the testicular parenchyma (in 3 different areas) concerning the central positioning of the mediastinum testis (the most hyperechoic part of the testis) (Figure 2).

Statistical Analysis
The obtained data concerning the testicular hemodynamics (PSV, EDV, RI, PI, and TAMAX), TE and PH, and biochemical analysis (NOMs and TAC) are presented as means ± SEM. The normality and homogeneity of the results were tested using the Kolmogorov-Smirnov test and chi-squared test, respectively. The differences between rams regarding the right and left testes were nonsignificant; therefore, the obtained results were pooled, and the comparisons were done between the different studied time points. Repeated measures ANOVA test was used for discrimination of the differences among the means in the studied time points, and finally with Bonferroni post hoc test. GraphPad Prism5 software was used for all the studied statistical assessments. P < 0.05 was considered as statistically significant.

Effects of LC Administration on Blood Flow Hemodynamic Patterns
The temporal changes in testicular blood flow patterns of the STA of heat-stressed rams administered LC in this study are Frontiers in Veterinary Science | www.frontiersin.org

Effects of LC Administration on Testicular Echogenic Properties
The changes in echogenicity of the testicular parenchyma after LC treatment concerning the studied time points in this study are given in Table 2. There were significant (P < 0.05) increases in the values of pixel intensity of the testicular parenchyma, especially at 1 and 168 h after LC administration (78.71 ± 2.50 and 88.56 ± 4.10, respectively), compared to just before administration (69.40 ± 4.75). While the PH of the testicular tissue did not exhibit any significant (P < 0.05) changes throughout the study time points.

Effect of LC Administration on Total Antioxidant Capacity and Nitric Oxide Concentrations
The serum total antioxidant capacity and nitric oxide levels in heat-stressed rams administered LC in this work are shown in Table 3. The NO levels in the rams' serum tended to increase (P = 0.07) after administration of LC compared to just before administration. Whereas the total antioxidant capacity showed significant (P < 0.05) gradual increases and reached the highest values at 168 h (2.75 ± 0.58 mM/l) after LC administration, compared to 0 h (1.12 ± 0.05 mM/l).

DISCUSSION
Male reproductive potential under HS conditions faces several obstacles, namely, sperm production decrement, OS, cellular apoptosis, steroidogenesis deficit, testicular hemodynamic disruption, and fertility decline (40). Several trials have been adopted to overcome the negative impact of heat stress in different species by using antioxidants (41)(42)(43)(44). L-carnitine, a natural antioxidant, was used extensively in the perspective of cellular bioenergetics, glucose uptake, steroidogenesis, and antiapoptosis under different environmental conditions in different species (26,29,30,35); however and interestingly, this study is the first, according to the best of the authors' update, to use LC for alleviation the adverse effects of heat stress on testicular blood flow (TBF), TE, nitric oxide (NO), and total antioxidants (TAC) concentrations in Ossimi rams. This study showed that treatment of heat-stressed Ossimi rams with LC exerted a significant improvement in testicular blood perfusion. Interpretation of testicular blood flow enhancement is based on RI, PI, PSV, and TAMAX decreases, which explain the elevation of arterial blood perfusion and decrease of the vessel resistance to blood flow. These changes warrant higher testicular blood perfusion (45,46). The way how LC improved TBF was not certainly clarified in this work; however, many pathways can describe these actions. First, LC may improve TBF through its cardioprotective effect on the myocardium, via the promotion of pyruvate oxidation, even at ischemic injury conditions, and helps the heart to recover properly, which affects directly the testicular irrigation with blood (47,48). Moreover, LC protects the cardiac function through its antiapoptotic properties of the vascular endothelium, evidenced by upregulation of Bcl2 (antiapoptotic) and downregulation of BAX (apoptotic) genes and insulin-like growth factor-1 (49). It would be quite interesting to investigate different parameters of the cardiac and vascular performance regarding the possible testicular blood modulations induced by LC administration in further studies. Healthy vascular endothelial cells produce optimum nitric oxide (potent vasodilator) concentrations resulting in higher blood flow to the reproductive tract (47). Also, LC has a direct effect on NO production in the pulmonary endothelial cells via the carnitine acetyltransferase enzyme (50). In addition, LC deficiency induces mitochondrial dysfunction, lessens ATP production and vascular NO synthesis (51). Surprisingly, the differences in NO concentrations between the studied time points in this study were nonsignificant but tended to increase (P = 0.07). Earlier studies in men indicated that administration of LC either through oral or parenteral routes provoked NO bioavailability (52,53). This discrepancy might be due to the differences in the dose (around 1 g/ram), frequency (single dose), and route of administration (IV) of LC treatment in this study compared to the aforementioned reports. The selected dose might be insufficient to elevate the NO concentration, as 1 g of LC may be enough for exerting antioxidant capacity and protecting the cellular components against lipid peroxidation (53), as proposed by the obtained results; whereas, for the elevation of NO, LC should be administered at a dose of at least 3 g daily (52). Second, the role of LC in mitochondrial functions should be considered, through fatty acid transfer, energy production, and removal of excess acyl-CoA, promoting optimum cellular functions in the whole body organs including heart and testes (26,42,48). Third, LC as a potent antioxidant protects the testicular cells from the free radicals (SOA and hydroxyl radicals) attacks by capturing them (26), these radicals stimulate the nitric oxide conversion to peroxynitrite (54), thus decreasing its bioavailability which, in turn, lead to decrease TBF. Fourth, LC may affect hypothalamic functions, manifested by elevated blood FSH and LH concentrations which, in turn, modulate testicular hemodynamics and functions (29,32).
In Ossimi rams, decreased testicular blood flow was reported in the summer season (10). However, there were recent studies indicating a marked increase in testicular blood flow to compensate for increased testicular metabolism under HS conditions (55)(56)(57). Indeed, heat stress induces increases in testicular blood perfusion if the heat stress is locally induced on the testes directly (55,57). However, in our experiment, the rams were exposed to thermal environmental conditions for about 3-4 weeks before the onset of the experiment (summer season). Second, our results may not be consistent with the findings in the above-mentioned literature, as these changes are dependent on whether the testicular temperature exceeded the core body temperature or not. In this study, rams were exposed to ambient temperature (>36 • C) and relative humidity (>60%) with temperature-humidity index (32.5) that their effect might be generalized on different body systems. Moreover, the difference in rams' breed adaptability to the thermal conditions may be another factor that should be considered.
Studying the testicular echogenicity provides a complementary noninvasive testicular function evaluation through pixel intensity calculation (21). In this study, there was a significant change in TEs; these changes might be due to increased testicular perfusion with blood. Moreover, the relation between the testicular echogenic and hemodynamic changes has been reported (25) and changes in TE affect the testicular functions (10,21,23). Explanation of increases in TE observed at 1 h might be attributed to the higher vascular irrigation. A recent study proposed that higher cellular condensation and vascular irrigation are correlated with higher echogenic changes of the testicular parenchyma of rams, and vice versa (58). At 168 h, increases in TE might be attributed to the improvement of total antioxidant capacity compared to 0 h with subsequent amelioration of the OS status that may enhance the functionality of the cellular matrix of the testis including Sertoli, Leydig, and the spermatogenic cells (59).
Heat-stressed rams treated with LC, in this study, experienced significant (P < 0.05) higher concentrations of plasma TAC. These findings are in line with many studies conducted in different animal species (26,53,60). The improvement in reproductive antioxidant defense systems may be due to the increase in reduced glutathione concentration and activities of glutathione peroxidase, catalase, and SOD enzymes (28,61,62), which protect the reproductive organs' defense systems against ROS attacks.
The authors thought that comparing the studied parameters before and after administration of LC is appropriate to test the effects of LC on the same individual of rams (to avoid individual variations). Moreover, to validate the results, the experimental procedures were repeated three times at a 2-week interval in the same conditions. However, the inclusion of the control group in the experimental protocol and examination of a big number of rams may be important to be conducted in a further study. In addition, clarification of the effects of different doses, protocols, routes, and forms of LC on testicular functionality, semen quality, and molecular basics of how LC affects TBF in rams in adverse conditions are valuable to be conducted.

CONCLUSION
A single intravenous administration of LC improves testicular hemodynamics (by lessening RI and PI of supra-testicular arteries) and TEs and was followed by an increase in total antioxidants concentrations in Ossimi rams during the heatstress conditions. However, further studies are needed to be conducted to clarify the effects of different doses, protocols, routes, and forms of LC on testicular functionality, semen quality, and molecular basics of how LC affects TBF in rams in adverse conditions.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

ETHICS STATEMENT
The animal study was reviewed and approved by the Ethical Committee for Animal Use, Cairo University, Giza Governorate, Egypt.