Production of transgenic first filial puppies expressing mutated human amyloid precursor protein gene

Propagation of transgenic animals by germline transmission using assisted reproductive technologies such as in vitro fertilization (IVF) is the most efficient way to produce transgenic colonies for biomedical research. The objective of this study was to generate transgenic puppies from a founder dog expressing the mutated human amyloid precursor protein (mhAPP) gene. Experiment I assessed the characteristics of the semen prepared by freshly diluted, swim-up, and Percoll gradient methods using a computer-assisted semen analyzer (CASA). Motile and progressively motile sperm counts were higher in the Percoll gradient samples (p < 0.05) than in the swim-up and freshly diluted samples. In Experiment II, a total of 59, 70, and 65 presumptive zygotes produced by fresh, Percoll gradient, and swim-up methods, respectively, were transferred to surrogates (5 for each group); the Percoll gradient (27.27%) and swim-up samples (14.29%) showed the highest blastocyst formation rates, while fresh diluted semen did not produce any blastocyst. Experiment III examined the full-term developmental ability of embryos. Among the 5 surrogates in the Percoll gradient group, one (20.0%) became pregnant; it had 4 (6.15%) sacs and delivered 4 (6.15%; 2 males and 2 females) live puppies. Among the 4 puppies, 2 (50.0%) were found to transmit the transgene on their nail and toe under GFP fluorescence. Furthermore, the integration and expression of the mhAPP transgene were examined in the umbilical cords of all the IVF-derived puppies, and the presence of the transgene was only observed in the GFP-positive puppies. Thus, semen prepared by the Percoll method could generate transgenic puppies by male germline transmission using the IVF technique. Our result will help propagate transgenic dogs efficiently, which will foster human biomedical research.


Introduction
Transgenic animals are useful in studying human diseases as they can provide deep insights into the molecular mechanisms contributing to the pathogenesis of specific diseases.It could assist in developing improved treatment regimens by identifying agents that can abrogate the onset of the disease, slow its progression, or alleviate its symptoms.However, selecting appropriate animals is vital in disease model experiments as closer genetic and physiological similarities with humans could generate more valuable and usable information (1).Although mice are widely used in transgenic models and have contributed immensely to human biomedical science, the results obtained from the mouse model cannot directly apply to humans due to huge physiological and genetic differences (2).Canine models can overcome some of those issues as they have close physiological and genetic similarities with humans (3).Furthermore, dogs live with humans in the same environment and share similar food, which makes them unique candidates.Dogs exhibit more than 600 genetic defects, and of those, 400 have a high degree of similarity with humans (4,5).In particular, transgenic dogs are a powerful model for preclinical assessment of the safety and efficacy of stem cell or gene therapy for human diseases.
The first transgenic animals were generated by pronuclear microinjection (6).After that, various methods such as spermmediated gene transfer (7), lentiviral transgene delivery (8), and CRISPR/Cas9 system (9) were used to produce transgenic animals.Somatic cell nuclear transfer (SCNT) is the most versatile tool for producing transgenic animals as it has greater efficiency (10).Using the SCNT, we generated transgenic dogs overexpressing mutated human amyloid precursor protein (mhAPP) (11).Dogs overexpressing the mhAPP gene may be used to understand the pathological and developmental characteristics of Alzheimer's disease in humans (12).It is essential to propagate the founder animals to develop specialized transgenic colonies as many animals are needed for biomedical research to avoid statistical bias and to obtain more precise information.SCNT can produce many duplicates of genetically engineered animals bypassing conventional breeding procedures.However, some reproductive complexities, such as ovulating immature oocytes and lack of a reliable in vitro maturation system, make canine cloning more challenging than other species (13,14).The time, cost, and procedural intricacy related to SCNT could be reduced significantly if transgenic puppies could be obtained from founder transgenic dogs using natural breeding or assisted reproductive technologies (ART).
The reproductive abilities of male and female transgenic dogs have been thoroughly investigated, and it is reported that they have standard reproductive capabilities (15).Hence, they can be propagated naturally by germline transmission, where transgene can be incorporated with sperm or oocytes and genetically transferred to their descendants.Germline transmission is the most efficient way to produce transgenic colonies in a shorter period at a relatively low cost (15).The first transgenic puppy produced by germline transmission by natural mating was reported by Hong et al. (15); they produced offspring using cloned transgenic female dogs.However, male transgenic animals are more suitable because when transgenic semen is combined with IVF technologies, it is possible to produce transgenic offspring more efficiently.To the best of our knowledge, no studies have described the successful germline transmission of transgenes to progeny using the IVF technique in canines.
IVF is a well-established reproductive technique that can be used in mammals for a variety of reasons.In humans, it is primarily used to overcome male or female infertility-related problems (16).In farm animals, an increase in selection intensity is the leading cause of IVF.Although puppies have been born from IVF before (17), the overall usage of IVF in canines is far lower than that of other mammals.Successful IVF requires an improved sperm preparation method as semen may have both functional and morphological abnormal spermatozoa.Sperm preparation methods that improve the selection of spermatozoa with normal morphology, intact acrosomes, high curvilinear or straight-line velocity, and linearity are positively correlated with successful IVF (18).Various sperm separation methods have been developed and successfully used for decades in different species.Among them, the swim-up and Percoll gradient methods are the most widely applied (16,19).However, the results are not always consistent.Mammalian spermatozoa differ in size, shape, and weight and species-specific differences have been reported.A comparison of these common sperm preparation methods still needs to be performed for canines.This study aimed to produce transgenic puppies by germline transmission using IVF techniques and compare two semen preparation methods for canine IVF, the Percoll gradient, and swim-up methods using a computer-assisted semen analyzer (CASA, Hamilton Thorne v.12.4 Build 006A HTC).

Care and management of animals
A total of 12 mixed-breed pro-estrus dogs aged between 1 and 7 years with a body weight of 20-25 kg were obtained from a breeder.Among them, seven dogs were used as oocyte donors, and five were recipients.During experiments, dogs were reared in indoor kennels (2.5 × 1.5 m).The pregnant recipients were kept in the research facility, and non-pregnant and oocyte donors were returned to the breeder.Commercial food was given to dogs once daily, and water was provided ad libitum.
Licensed veterinarians have performed all surgical procedures under general anesthesia.All surgical procedures were conducted following the animal study guidelines, which the ethics committee approved at the Abu Dhabi Biotech Research Foundation, Republic of Korea (Permit no.C-16-01).We used propofol (1 mg/kg body weight) to induce the anesthesia and 2% isoflurane inhalation to maintain the anesthesia.

Chemicals and reagents
Unless otherwise mentioned, we purchased all chemicals and reagents from Sigma-Aldrich (St. Louis, MO, United States).

Collection of in vivo matured oocyte
Ovulation time was determined by measuring the serum progesterone concentration once daily and by ovarian ultrasonography (20,21).The in vivo matured oocytes were collected by flushing both fallopian tubes at 72-84 h after ovulation.The reproductive tracts were accessed by mid-ventral laparotomy.The flushing medium was

Semen preparation
A 27-month-old transgenic dog expressing the mhAPP gene was used as the semen donor.The dog was clinically healthy, and the semen parameter was within the normal range.After 3 days of abstinence, the second sperm-rich fraction of ejaculate was collected by manual stimulation.Semen was washed by centrifugation at 750 × g for 5 min in wash-TALP (TALP: Tyrode's albumin lactate pyruvate) and divided into three fractions.Semen was then prepared for IVF using three different methods: freshly diluted, Percoll gradient, and swim-up procedures for further experimentation.The composition of wash-TALP, Cap-TALP (capacitation-TALP), and IVF-TALP is given in Supplementary Table S1, which is a slight modification of Mahi and Yanagimachi (22) and Nagashima et al. (17).

Fresh dilution
The semen was centrifuged at 400 × g for 5 min to pellet the sperm.Sperm were then resuspended in Cap-TALP and incubated at a concentration of 7.5×10 6 sperm/mL for 4 h at 38°C with 5% CO 2 for capacitation.

Swim-up procedure
Semen was prepared for the swim-up procedure as previously described by Parrish et al. (23).Briefly, fresh semen (0.5 mL) was layered in duplicate (0.25 mL) under 1 mL of Cap-TALP and incubated for 30 min at 38°C in a humidified atmosphere of 5% CO 2 and 95% air.The top 0.80 mL of medium from each tube was collected, pooled, and centrifuged at 1000 × g for 10 min.The pellet was washed by centrifugation in fresh Cap-TALP, the final pellet was reconstituted in 0.5 mL of Cap-TALP, and its concentration was adjusted to 7.5×10 6 sperm/mL.

Percoll gradient procedure
Semen was prepared for the swim-up procedure as previously described (23).A 90% (v/v) and 45% (v/v) isotonic Percoll solution was prepared by adding 5 mL of Cap-TALP (capacitation -TALP) to 45 mL of Percoll and 1 mL Cap-TALP to 1 mL of 90% Percoll, respectively.Percoll gradient was prepared in a 15 mL conical tube by adding 1 mL of 90% Percoll to the bottom of the tube and 2 mL of 45% Percoll on its top.Finally, 0.5 mL of semen was placed carefully on top of the Percoll gradient without mixing the layers.The tube was then centrifuged at 700 × g for 10 min.The pellet was diluted in 1 mL Cap-TALP, and the second washing was done by centrifugation at 700 × g for 5 min.The pellet was diluted by adjusting sperm concentration to 7.5×10 6 sperm/mL using Cap-TALP.

Measurement of sperm characteristics
Experiment I was conducted to assess the various characteristics of spermatozoa associated with their fertility in each group.Nine parameters, namely, motility, progressive motility, average path velocity (VAP), linear velocity (VSL), curvilinear velocity (VCL), straightness coefficient (STR), linear coefficient (LIN), mean amplitude of head lateral displacement (mALH), and frequency of head displacement (BCF), were measured using CASA (Hamilton Thorne Inc., Beverly, United States).This device consists of a phasecontrast microscope, a camera, a minitherm stage warmer, and an image digitizer.The settings for the canine sperm samples were established using the method described in Rijsselaere et al. (24).Sperm samples (8 μL) were placed in a cell chamber (Leja Products B.V., depth 20 μm) under a microscope, and the tracks from at least 1,000 sperm were recorded and evaluated.On average, 4 images were evaluated in 32 frames per analysis at 20 ms intervals.The Cell Motion Analyzer 2.0 software (CMA, Medical Technologies; Montreux, Switzerland) was used to evaluate the percentage of motile sperm and track velocities.

In vitro fertilization and embryo transfer
Prior to IVF, oocytes were washed several times in IVF-TALP.A group of 6-8 oocytes with homogenously dark cytoplasm and normal morphology in 6 μL of IVF-TALP were transferred into a 40 μL of IVF-TALP droplet.The IVF droplets were overlaid with light mineral oil; 4 μL of sperm suspension (3.0×10 4 sperm/ drop) was added to each droplet and co-incubated for 4 h at 5% CO2 and 38.5°C in humidified air.After 4 h, oocytes were pipetted gently in IVF-TALP medium to remove adherent sperm and transferred to pre-equilibrated IVF-TALP droplets.After co-incubation for 12 h, oocytes were denuded in TCM 199 supplemented with HEPES (4.7 mg/mL), 10% (v/v) FBS, and 1% (v/v) antibiotic-antimycotic.
The ovary was approached using a mid-ventral laparotomy.The fallopian tube corresponding to the ovary with more corpora lutea was selected for embryo transfer.Presumptive zygotes were loaded into a 3.5 Fr × 5.5" Sterile Tom Cat Catheter (Argyle™ Covidien, Dublin, Ireland) in 2-4 μL IVF-TALP and gently advanced 2.5 cm (2/3 distal position) into the oviduct via the infundibulum and zygotes were slowly dispensed.

Embryo collection
Experiment II was conducted to examine the in vivo pre-implantation development of the embryos.Embryos were collected on day 7 post-transfer (10 days after ovulation) as previously described (25).Briefly, a Foley balloon catheter (FBC) was used for flushing the reproductive tracts using a balloon tip catheter without excision.Uterine and oviduct flushings were performed under general anesthesia on day 7 post-embryo transfer (ET).After surgical exposure of the reproductive tract, a needle puncture was made in the uterine body using a fine needle, which was then removed and replaced with a balloon catheter caudal to the bifurcation of the uterus.After ligating the FBC into the cervix and inflating the balloon, the upper part of the designated segment of the uterus was ligated to prevent backflow and the needle was inserted allowing 10 mL TCM 199 supplemented with HEPES flushing medium to flow through three or four times.After collection, the perforation was closed using a single stitch.

Pregnancy diagnosis
In Experiment III, the full-term developmental ability of IVF-derived embryos was evaluated.Pregnancy was diagnosed using a portable ultrasound machine with a 3.5 MHz curved transducer (SonoAce R7; Samsung Medison, Seoul, Korea) after 25 days of ET in either the standing or dorsal recumbence position.Ultrasonography was repeated weekly to monitor fetal development.

Genomic DNA extraction, RT-qPCR
G-DEX™ II Genomic DNA Extraction kit (Intron Biotechnology, Suwon, South Korea) was used to isolate canine genomic DNA.After isolation, 100 ng of DNA was amplified using 10 pmol of specific primers in a 20 μL PCR mixture having 2× Lamp Taq PCR Master Mix 3 (BioFact, Daejeon, Korea).We used the PCR condition as follows: 35 denaturation cycles each for 30 s at 95°C, annealing for 30 s at 55°C after that, extension for 30 s at 72°C, and a final extension for 5 min at 72°C.We separated the PCR product on 1.5% agarose gel after staining with EcoDye™ Nucleic acid staining solution (BioFact, Daejeon, Korea).The photographs were taken using UV illumination, and images were scanned using a Gel Doc EQ system (Bio-Rad Laboratories, Inc., Hercules, CA, United States).
The relative copy number of the mhAPP gene in 4 transgenic puppies was compared to the original transgenic semen donor dog (SBM16).The experiment was repeated 3 times in a 20 μL reaction volume mixture consisting of 2× SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) and 10 pmol of specific primers on a 7,500 real-time PCR System (Applied Biosystems, Foster City, CA, United States).We used 1 cycle of 10 min at 95°C, and 40 cycles of 15 s at 95°C, 15 s at 55°C, and 30 s at 72°C as amplification parameters for real-time quantitative PCR (RT-qPCR).At the end of the amplification, a melting curve analysis was recorded to confirm that there was no contamination by primer dimers.Standard curves were established using 5 serial dilutions of a reference plasmid.Fluorescence was acquired to determine the threshold cycle during the log-linear phase of the reaction at which fluorescence rose above the background.The DNA concentration was measured by using a 7,500 real-time PCR (qPCR).The qPCR uses real-time fluorescence to measure the quantity of DNA present at each cycle during PCR.Data obtained by qPCR were plotted in Excel, and the ΔΔCt method was used to calculate the relative concentration of gene expressed compared with GAPDH.Details of the primers used in this study are presented in Table 1.

Statistical analysis
We used the SPSS statistical program (SPSS Inc., Chicago, IL, United States) for statistical analyses.A one-way analysis of variance (ANOVA) followed by Duncan's post-hoc test was used to analyze the sperm parameters, pre-implantation developmental competence of IVF-derived embryos, and relative mhAPP copy number.Blastocyst quality and pregnancy rate were compared using Pearson's chi-square test and Fisher's exact test.The normal distribution of data was evaluated before performing statistical analysis.GraphPad Prism (version 4.0; GraphPad Software, San Diego, CA) was used for the graphical presentation of data.Data were represented as mean ± standard error, and p-values less than 0.05 were considered statistically significant.

Semen parameters
Before the commencement of the experiment, the founder dog was evaluated for the basic semen parameters.The volume of semen, the concentration of spermatozoa, %motility, and %progressive motility were 2.57 ± 0.23 mL, 1.73 ± 0.37 × 10 8 /mL, 77.33 ± 3.93%, and 62.67 ± 1.86%, respectively, for the founder dog.The values are the mean ± SE of three replications.
During the experiment, nine parameters, namely, motility, progressive motility, VAP, VSL, VCL, STR, LIN, mALH, and BCF were measured in the freshly diluted, Percoll gradient, and swim-up treatment groups and are presented in Figure 1.All parameters were significantly higher for the spermatozoa in the Percoll gradient and swim-up groups than those in the freshly diluted semen groups (p < 0.001).

Developmental competence of the IVF-derived embryos
To determine the pre-implantation developmental competence of the embryos generated by IVF, presumptive zygotes at the one-cell stage were transferred to surrogates with an estrous cycle similar to that of the egg donor.Embryos were recovered after 7 days, and their stage was categorized as cleaved (two-cell stage), morula, and blastocyst stage (Table 2).There were no significant differences in embryo recovery and development up to the morula stage among the different groups.
The blastocyst formation rate was significantly higher in the Percoll gradient (27.27%) and swim-up (14.29%) groups than in the freshly diluted group (0%; p < 0.05).Representative images of embryos and blastocysts derived by the IVF are shown in Figure 2. To determine the quality of the blastocysts, they were stained with Hoechst 33258, and the total cell numbers were counted.The average cell numbers in the blastocysts were 259.75 ± 26.24 and 237.41 ± 31.74 for the Percoll and swim-up, respectively (2G).However, the difference between these groups was not statistically significant.

Post-implantation developmental competence
The basic parameters for producing transgenic puppies are summarized in Table 3.We have retrieved 86 oocytes (12.29 ± 1.10/ dog) from 7 dogs; 65 presumptive zygotes were transferred to 5 dogs (13.4 ± 1.03 zygotes/ recipients) in the Percoll group and 1 recipient (20.0%) became pregnant.It had four (6.15%) sacs and delivered four (6.15%) live puppies by natural birth.Among the four puppies, two were female and two were male (Table 4).The birth weight was 575 g (AF, Alzheimer's female AF1), 460 g (AF2), 550 g (AM, Alzheimer's male AM3), and 450 g (AM4), respectively.The first parturition was initiated late on the 59th day post-embryo transfer and continued until early morning on the 60th day, leading to a discrepancy in the full-term gestation period between the first parturition and the others.No pregnancy was established in the swim-up group.

Germline transmission and parentage analysis
Among the four puppies, two (50.0%) were found to transmit the transgene as observed by GFP fluorescence on the nail and toe (Figure 3).The integration and expression of the mhAPP transgene in the puppies were examined in the umbilical cords of all the IVF-derived puppies.The presence of the transgene (Figure 4A) and their expression (Figure 4B) were only observed in the GFP-positive puppies (AF1 and AF2 for the mhAPP gene).Microsatellite analysis of 18 canine loci revealed that the IVF-derived offspring were identical to their sperm and oocyte donors (raw data can be obtained by requesting the corresponding author).

Discussion
Our ultimate objective is to standardize a practical approach to generating transgenic puppies from transgenic founder dogs by germline transmission for biomedical research.SCNT offers a unique opportunity for the production of genetically modified animals.The first transgenic dog expressing red fluorescent protein was produced by the SCNT technique (15).We have reported the production of two transgenic puppies expressing the human phosphoenolpyruvate carboxykinase (hPEPCK) and the mhAPP gene using the SCNT procedure (11,26).Herein, we report the production of mhAPP transgenic puppies using germline transmission using IVF.
SCNT is a widely used and reliable technique to produce transgenic animals.However, it is a highly technical, time-consuming, and costly procedure.Deliberate efforts were made to overcome these issues related to SCNT.The most favorable solution to produce many transgenic animals in a shorter time could be producing a founder animal by SCNT and propagating that founder animal by germline transmission either by natural mating or by assisted reproductive techniques such as artificial insemination and IVF.Germline transmission could be a great help in the mass production of transgenic animals for biomedical research as it simplifies the production process and significantly reduces the time and cost of cloning.The reproductive performances of both cloned and transgenic animals are reported to be similar to aged-matched non-cloned animals.Park et al. ( 27) evaluated the reproductive activity of male and female cloned dogs.They reported that male cloned dogs had sperm motility parameters within the reference range, and cloned female dogs had standard patterns of reproductive hormones.Furthermore, viable puppies were obtained when cloned females were artificially inseminated using semen collected from a cloned dog.
The motility of sperm, especially progressive motility, is a commonly used parameter for semen quality.In addition to motility, another vigor parameter, such as velocity (average path, curvilinear, or straight), is also essential in predicting the fertilizing capacity of sperm.In this study, we used CASA to assess the quality of semen specimens prepared using three different methods.Rijsselaere et al. (24) reported that with proper internal image settings, this analyzer could objectively assess canine semen quality for research and practical purposes.CASA can significantly improve the accuracy and precision of dog semen analysis methods.Furthermore, CASA has been successfully used in other mammalian species, such as bulls, boars, and humans, to improve IVF outcomes (28,29).
Several reports describing mammalian sperm preparation using swim-up and Percoll gradient methods have been published (28, 29).However, these reports are inconsistent.Percoll separation has been reported to be more effective for selecting highly motile sperm in cattle (23), but buffalo and stallion sperm were found of higher quality when prepared using the swim-up method (30,31).A direct comparison of the efficiency of motile spermatozoa separation from the same sample has not been conducted in dogs.Our study revealed that both the swim-up and Percoll methods separated the highest number of motile and progressively motile sperm, compared with freshly diluent semen.
In this study, freshly diluted semen failed to produce any blastocysts, while the swim-up method produced 14.29% blastocysts.However, no pregnancy was established.Freshly diluted semen may have much more debris and abnormal or dead spermatozoa which are reflected in less motility, progressive motility, and other parameters.Ultimately, no blastocyst was produced from freshly diluted semen.The Percoll method produced 27.27% blastocyst; successful pregnancy and live puppies were born in this method.Only the Percoll method was shown to produce live offspring in this study.Motility and progressive motility were better for the Percoll-prepared spermatozoa than the swim-up procedure.Higher blastocyst rates in the Percoll protocol may result in successful pregnancies.It is worth mentioning that the pregnancy rate following artificial insemination is significantly influenced by the quality of the semen, especially with regard to the motility and progressiveness of sperm (32).Ren et al. (33) compared four different methods of semen preparation and reported the highest pregnancy rates following intrauterine insemination using sperm prepared via the Percoll method.They argued that the Percoll method produced sperm with superior VAP and VSL, which might explain the higher pregnancy rates.
The availability of mature oocytes is the main limiting factor for biotechnological research in canines.In vivo, matured oocytes are considered the primary source of oocytes as a reliable in vitro system for canines is yet to be developed (13,14).Mammals usually ovulate mature oocytes, while dogs ovulate immature oocytes at their germinal vesicle stage of development (34).As a result, oocytes need 3 to 5 days in the fallopian tube to reach the metaphase II stage, which is considered mature oocytes (35).Therefore, mature canine oocytes are collected by flushing the fallopian tubes (20).In the present experiment, we also collected in vivo matured oocytes from naturally cyclic bitches by flushing the fallopian tubes, and we retrieved, on average, 12.29 oocytes per dog.Nagashima et al. (17) collected in vivo matured oocytes by flushing the excised oviducts of 46 beagle dogs and reported an average of 4.77 oocytes per dog, which is lower than our retrieval rate.The differences in oocyte recovery may be due to breed variation.Nagashima et al. (17) used purebred beagle dog, which is medium-sized, while we used mixed-breed dogs, much larger than beagle dogs.
The first live puppy generated from IVF-derived embryos was reported by Nagashima et al. (17), where they emphasized using in vivo matured oocytes and suitable protocol for sperm preparation.We reported here that only the Percoll gradient method was able to produce transgenic puppies by germline transmission; 4 live puppies (6.15%) were born from 1 pregnant recipient out of 5 recipients.We observed 50.0%germline transmission to the F1 generation; among the 4 puppies, 2 were found to transmit GFP on their nail and toe.Furthermore, as revealed on RT-PCR, GFP-positive puppies showed genomic integration of mhAPP transgene.Puppies expressing the mhAPP gene could produce Alzheimer's disease-like symptoms such as cognitive dysfunction.These transgenic dogs are useful for understanding the developmental and pathological phenomena of Alzheimer's disease and hence helpful in the development of novel therapies.Germline transmission in cloned transgenic animals is reported in various species, such as bovine (36), porcine (37), caprine (38), and marmoset (39).Hong et al. (15) reported that 52.6% of F1 and F2 offspring showed emission of red fluorescence protein in their skin and claws, which were readily apparent even under bright-field illumination.
The birth weight of our transgenic puppies was from 450 g to 575 g.They were healthy, and no congenital anomalies were detected.However, in the course of the experiment, one puppy (AM2) died at the age of 9 days.Post-mortem findings revealed the cause of death as non-specific.It is unlikely that the death of this puppy was due to the detrimental effect of the transgene because the other siblings carrying the same transgene are still alive and healthy.

Conclusion
Successful germline transmission of a transgene in canines could be obtained using the IVF technique.Semen prepared by the Percoll method can produce live transgenic puppies by germline transmission as two out of four offspring showed integration of the transgene into their genome.Our result will benefit the propagation of transgenic dogs easily, economically, and efficiently, which will foster disease model studies in humans.
rows but in the same column marked with a superscript are significantly different (p < 0.05).b The percentage of embryos recovered was calculated based on the number of embryos transferred.cThepercentage of embryos developed in each stage was calculated relative to the number of recovered embryos.

FIGURE 2
FIGURE 2 In vivo collection of oocytes and embryos at different developmental stages.(A) In vivo collected oocytes; (B) two-cell embryos; (C) four-cell embryos; (D) morula stage embryos; (E) blastocyst under bright field; (F) blastocyst under fluorescence light; (G) cell number in the blastocyst.Scale bar = 100 μM.

TABLE 1
List of primers.

TABLE 2
Pre-implantation developmental competence of IVF-derived canine embryos in vivo.

TABLE 3
Production rates of transgenic F1 puppies.Pregnancy rate was calculated relative to the number of surrogates.b Offspring born was calculated relative to the number of embryos transferred. a

TABLE 4
Pregnancy parameter and GFP expression of IVF offspring.