Effects of dietary Astragalus membranaceus and Codonopsis pilosula extracts on growth performance, antioxidant capacity, immune status, and intestinal health in broilers

The objective of this study was to examine the effects of dietary Chinese herbal medicine (CHM) consisting of Astragalus membranaceus (Fisch.) Bunge (AMT) and Codonopsis pilosula (Franch.) Nannf (CPO) extracts on growth performance, antioxidant capacity, immune status, and intestinal health of broiler chickens. Two groups were formed, each consisting of six replicates of 12 one-day-old healthy male 817 white feather broilers. Broilers were fed either a basal diet (CON group) or a basal diet supplemented with 500 mg/kg CHM. The trial lasted 50 days. The results showed that CHM supplementation resulted in enhanced feed efficiency and antioxidant capacity in both the serum and liver, while it reduced uric acid and endotoxin levels, as well as diamine oxidase activity (p < 0.05). Additionally, CHM treatment increased the height of jejunum villi and upregulated Claudin-1 expression in the jejunal mucosa accompanied by an increase in the mRNA levels of interleukin-6 (IL-6), interferon-γ (IFN-γ), interferon-β (IFN-β), tumor necrosis factor-α (TNF-α), and anti-inflammatory cytokine interleukin-10 (IL-10) (p < 0.05). The presence of dietary CHM caused an increase in the proportions of Bacteroidetes and unclassified Bacteroidales but led to a decrease in those of Firmicutes and Alistipes (p < 0.05). The composition of the jejunal mucosa microbiota was correlated with the feed conversion ratio, serum metabolites, and gene expression based on Spearman correlation analysis. The findings indicated that the consumption of dietary CHM improved the utilization of feed, increased the mRNA expression of pro-inflammatory cytokines in the jejunal mucosa, and decreased the endotoxin level and activities of diamine oxidase and lactate dehydrogenase in the serum, which could potentially be linked to changes in the gut microbiota of broiler chickens.


Introduction
In recent years, chicken meat has been in high demand as a source of protein.Consequently, commercial chicken farms prioritize faster growth and higher feed efficiency as their primary objectives.However, attaining these objectives requires ensuring the health of the chickens, good digestion and absorption, and protection against pathogens.Owing to the exhaustive ban on antibiotics in feed, broiler chickens have become increasingly vulnerable to illnesses, resulting in diminished performance as they face the threat of contagious agents, stressors, and nutritional insufficiencies (1,2).Hence, many researchers are focusing on finding effective solutions to address or mitigate this issue.
Chinese herbal medicines (CHM) and their extracts have been utilized as adjuvants to mitigate the risk of disease and enhance the growth performance of livestock (3)(4)(5).The profusion of herbal remedies, Astragalus membranaceus (Fisch.)Bunge (AMT) and Codonopsis pilosula (Franch.)Nannf (CPO) has been used in traditional oriental medicine.AMT, one of the most widely used tonic herbs in many Asian regions, has been reported to possess components that demonstrate various biological effects, including antioxidative, anti-inflammatory, and antiviral properties (6)(7)(8).AMT has been reported to scavenge oxygen free radicals, making it an important plant in preventing mucosal injury in the intestine, liver, and plasma (9).CPO is known for its ability to enhance spleen function, promote liver health, and exhibit anti-tumor, antioxidant, and antibacterial effects (10)(11)(12).Studies have also shown that AMT and CPO have immunomodulatory effect, improve immune defense function of broiler chickens and mice, and have nourishing effects and can be used as immune adjuvants (13,14).These two plants are often used in tandem to combat multiple of diseases because their synergistic effects are believed to promote lipid oxidation, protect against illnesses, and ameliorate inflammation (15,16).
Recently, researchers found that the combination of CPO and AMT could re-establish the immune balance of the intestinal flora and alleviate colonic mucosal injury in mice with colitis (16).Synergistic extracts of AMT and CPO showed antioxidant activity in weaned piglets and influenced the relative proportion of Firmicutes and Bacteroidetes in the gastrointestinal tract (17).However, to the best of our knowledge, the combined effects of AMT and CPO extracts in broiler chickens have not yet been thoroughly studied.Therefore, the aim of this study was to explore the effects of adding CHM, by considering the example of AMT and CPO extracts, to the diet on the growth performance, immune response, antioxidant capacity, and intestinal health of broiler chickens.

Experimental design, birds, diets, and management
A total of 144 one-day-old male 817 white feather broilers (Nanhai little white chicken) with similar initial weights were purchased from Muyuan Foods Co., Ltd., (Guangzhou, China).The broilers were allocated to two groups, with six replicates per group and 12 chicks per replicate.Birds were fed with either a basal diet or a basal diet supplemented with 500 mg/kg CHM.The determination of the CHM dosage was based on a preliminary experiment conducted by the research group members, taking into account the dosage of extracts mentioned in our previous study (18).The CHM product was purchased from Guangdong Huakang Biopharmaceutical Co., LTD (Guangzhou, China), which mainly consists of a mixture of extracts of AMT and CPO.The main component of this product is polysaccharide.The diet was developed based on the nutrient needs of broiler chickens (Table 1), ensuring that all essential nutrients were provided in amounts that met or surpassed the requirements set for chickens (NRC, 1994).In this study, all the chicks were kept in a controlled environment with specific dark-and-light cycles.All chicks were subjected to the same light cycle arrangement (24 L:0 D) 24 h before the experiment.From the second day of age, the brightness gradually decreased and reached 18 L:6 D on the seventh day.From the eighth day onwards, the light cycle was maintained at 16 L:8 D until the end of the experiment.Furthermore, the chicks were provided with unrestricted access to both water and feed throughout the entire duration of the trial.

Sample collection
On the 50th day, after an eight-hour fasting period, measurements were made of the body weight (BW) and the amount of feed remaining in each replicate.Afterwards, the growth performance parameters for each replicate were computed, which included the mean average daily feed intake (ADFI), mean average daily gain (ADG), and the feed conversion ratio (FCR).FCR = feed intake (g) / weight gain (g).Additionally, any instances of mortality throughout the entire 50-day duration of the experiment were taken into consideration.One chicken was selected from each replicate after weighting at d 50 for blood and tissue samples collection.Following the extraction of around 4 mL of blood from the brachial vein, it was subsequently centrifuged at a temperature of 4°C for 15 min with a force of 3,000 g.After centrifugation, serum was stored at −80°C for further analysis.Following blood collection, chickens were sacrificed through exsanguination.Liver tissue samples were collected uniformly from the left side of each chicken, immediately placed in liquid nitrogen, and subsequently stored at −80°C to facilitate determination of antioxidant capacity.Furthermore, the jejunum, jejunal mucosa, and the contents of the cecum were systematically collected and preserved using different techniques.Both the jejunal mucosa and jejunal tissue samples were obtained from the middle section of the intestinal segment.The jejunal mucosa was collected by scraping the jejunum using a scalpel.Additionally, the scraped jejunal tissue, measuring approximately 3-4 cm in length, was preserved for further analysis.Histomorphological analyses were conducted on sections of scraped jejunal tissue that were preserved in formaldehyde.

Serum and liver antioxidant capacity
The antioxidant levels in serum and livers were assessed with kits to measure various indicators, including total superoxide dismutase (T-SOD), malondialdehyde (MDA), catalase (CAT), total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px).The kits were purchased from Nanjing Jiancheng BioEngineering, and the determination procedure was conducted following the kits' instructions.Before homogenizing with nine times normal saline, the liver samples were thawed on ice, maintaining a ratio of 1 gram of tissue to 9 milli-liters of saline.The website of Nanjing Jiancheng company has a technical support section that offers details about technical approaches. 1 After centrifugation, the supernatant from liver homogenate was obtained for determining and analyzing the index.

Intestinal tissue morphology
Morphology is an accepted method for studying the change of tissue structure, such as livers (19), hearts (20), and jejunum (21).In this study, tissue specimens from the jejunum were collected and preserved in a formaldehyde solution for further analysis.Afterwards, the samples were immersed in paraffin and sliced into segments.The serial sections that were affixed onto a slide, which was then treated with hematoxylin and eosin staining.Six complete crypto-villi units were selected from each sample for analysis.Measurements of the small intestine villi and crypts were performed using the highdefinition LEICA imaging system (version DFC290, Heilbrugger, Switzerland).To assess the morphology of the tissue, measurements of villus height and crypt depth were taken.The measurement of villi height starts from the villi tip and ends at the junction of the villi recess, while crypt depth refers to the depth of the invagination between neighboring villi.Calculate the villus/crypt ratio by dividing the villi height by the crypt depth.This ratio provides insight into the structural integrity of the intestinal tissue.

Quantitative PCR
We used TransZol reagent (TransGen Biotech, Beijing, China) to extract RNA from jejunal mucosa and with the NanoDrop 2000C Ultramicrospectrophotometer (Thermo, Shanghai, China) to measure the quality of the RNA (OD 260/280).RNA was reverse-transcribed by RT EasyTMII Kit, and cDNA was synthesized.A specific primer sequence was combined with cDNA, and real-time quantitative PCR was conducted to analyze cDNA gene expression.The primers were created with the assistance of the NCBI primer tool and their specificities were confirmed.Qingke Biological Co., Ltd.synthesized the primers utilized in the research, and all pairs of primers exhibited an amplification efficiency of approximately 100% (Table 2).The PCR amplification system contained a total volume of 20 μL, and the cycling conditions consisted of 30 s at 95°C, followed by 10 s at 95°C and 30 s at 60°C for 40 cycles.To determine the relative gene

16S sequencing and cecal microbiota analysis
The 16 s rRNA sequencing of feces was entrusted to Lianchuan Biotechnology Co., LTD (Hangzhou, China).The hexadecyltrimethylammonium bromide (CTAB) method was used to extract genomic DNA from the contents of the cecum.The concentration of DNA was determined and the quality of the DNA extraction was evaluated using agarose gel electrophoresis, employing an ultraviolet spectrophotometer.DNA obtained from the samples was used to amplify the 16S rRNA V3-V4 region.PCR products were purified using AM-Pure XT beads from Beckman Coulter Genomics in Danvers, MA, USA.Subsequently, the quantification was performed using Qubit from Invitrogen in the United States.The refined PCR samples were evaluated using an Agilent 2,100 Bioanalyzer (Agilent, USA) and the Illumina Library Quantification Kit from Kapa Biosciences (Woburn, MA, USA).The samples obtained through sequencing were divided based on barcode information for the double-ended data.Data was spliced and filtered after the joint and barcode sequences were removed.Afterwards, dada 2 was used in conjunction with qiime DADA 2 denoise-paired to carry out length filtering and denoising, which led to the detection of ASV (feature) sequences and the generation of an ASV (feature) abundance chart.Singletons ASV were then removed.The acquired ASV (characteristic) sequences and ASV (characteristic) abundance table were utilized for the analysis of alpha diversity and beta diversity.The alpha diversity analysis assesses domestic diversity using six indexes: observed_ species, shannon, simpson, chao1, goods_coverage, and pielou_e.Additionally, four types of distances (unweighted_unifrac, weighted_ unifrac, jaccard, bray_curtis) were calculated to evaluate the diversity between habitats (samples/groups).The characterization of microorganismal features differentiating the fecal microbiota was performed using the linear discriminant analysis (LDA) effect size (LEfSe) method. 2 We analyzed the Spearman's correlations using the heatmap function from the R package (version 3.6.3).Spearman correlation methodology can be the website, 3 and use OmicStudio tools on 4 perform clustering correlation heat map with symbols (23).

Statistical analysis
All data was analyzed using independent samples t-test in SPSS version 23.0 software (SPSS, Inc., Chicago, IL, USA).The data were expressed as mean ± standard deviation (SD).Values at p < 0.05 were statistically significant and values at 0.05 < p < 0.10 are trending.

Growth performance
Data on growth performance are shown in Table 3.Compared with those in the CON group, the BW and ADG were higher in the CHM group; however, there were no significant differences observed among BW, ADG, and ADFI between the two groups from day 1 to day 50.However, FCR of the CHM group was lower than that of the CON group (p = 0.02).

Serum physiological and biochemical indexes
The effects of CHM on the physiological serum parameters of broilers are presented in Table 4. Compared with the CON group, the CHM group showed a significant increase in total cholesterol (p = 0.03) and HDL-C (p = 0.01) serum levels, while exhibiting a decrease in UA level (p = 0.01) and LDH activity (p = 0.03).Aspartate aminotransferase level (p = 0.05) was higher in the CHM group than in the CON group.In addition, the CHM group exhibited a significant decrease in DAO activity (p = 0.01) and endotoxin concentration (p = 0.04).Serum levels of triglycerides, low-density lipoprotein cholesterol, albumin, alkaline phosphatase, and alanine aminotransferase were not significantly affected by the experimental treatments.

Immune response
As shown in Table 5, serum levels of immune factors were determined using enzyme-linked immunosorbent assay (ELISA) kits, whereas mRNA levels of immune factors in the jejunal mucosa of broilers were quantified using quantitative polymerase chain reaction (qPCR).Compared with the CON group, the CHM group exhibited decreased levels of IL-10 (p = 0.08) in the serum.Moreover, birds in CHM group had increased mRNA expression levels of pro-inflammatory cytokines IL-6 (p = 0.02), interferon-γ (IFN-γ) (p = 0.01), interferon-β (IFN-β) (p = 0.01) and TNF-α (p = 0.01), as well as anti-inflammatory cytokine IL-10 (p = 0.01) in the jejunal mucosal tissue compared to the birds in the CON group.

Serum and liver antioxidant capacity
Compared with the CON group (Table 6), the CHM group exhibited a significant increase in serum antioxidant parameters, specifically T-AOC (p = 0.01), T-SOD (p = 0.02) and GSH-Px (p = 0.02) content.In addition, the CHM group showed significantly increased T-AOC (p = 0.01), T-SOD (p = 0.01), CAT (p = 0.04) and GSH-Px activity (p = 0.01) in the liver tissues compared to the CON group.

Intestinal physical barrier function
As shown in Table 7, CHM supplementation significantly increased the height of the jejunal villi (p = 0.01) and upregulated the mRNA expression level of Claudin-1 (p = 0.01) in the jejunal mucosa compared to those in the CON group.

Gut microbiota
Figure 1 revealed that CHM supplementation affected broiler cecal microbiota.According to the Venn diagram, the overall number of operational taxonomic units (OTUs) was 1,827, with 442 OTUs common to both groups (Figure 1A).Notably, 426 OTUs were found to be unique to the CHM group.To depict the microbiome space of various groups, we performed non-metric multidimensional scaling (NMDS) using weighted UniFrac and Bray-Curtis distances.The composition of the gut microbiome differed between the two groups (Figure 1B).In contrast to that in the CON group, the inclusion of CHM significantly decreased chao 1 (p < 0.01) and observed_otus (p < 0.01) measures of alpha diversity (Table 8).
Figures 1C-E displays the distribution of the cecal microbiota at the phylum and genus levels.Firmicutes and Bacteroidetes were the most prevalent phyla in the two groups (Figure 1C).CHM significantly increased the relative abundance of Bacteroidetes (p = 0.01; Figure 1E) and decreased Firmicutes compared to those in the CON group (p < 0.01; Figure 1E).Similarly, at the genus level, CHM had a significant effect on the abundance of Bacteroidales_unclassified (p = 0.01; Figure 1E) and caused a significant decrease in the abundance of Alistipes (p = 0.01; Figure 1E).

Correlation analysis of cecal microbiota, FCR and jejunal mucosal gene changes
Figure 2 displays the results of Spearman's correlation analysis, illustrating the relationship between the most prominent 20 genus and factors such as FCR, serum differential metabolites (DAO, HDL-C, LDH, UA, and endotoxin), and genes with significant differences in the jejunal mucosa.The abundance of Bacteroidales_ unclassified had a positive correlation with the expression of IFN-β, TNF-α, Claudin-1, and IL-6 mRNAs, while it had a negative correlation with FCR, DAO, LDH, and endotoxin.However, Alistipes showed a negative correlation with the mRNA expression of IFN-β, and a positive correlation with the FCR, DAO, LDH, and endotoxin.Meanwhile, Ruminococcaceae_unclassified exhibited a negative correlation with the mRNA expression of IFN-γ, TNF-α, IL-6, and IL-10, while showing a positive correlation with FCR.Furthermore, Clostridia_vadinBB60_group_unclassified showed a positive correlation with FCR, LDH and UA and a negative correlation with IL-10 expression.FCR is used in animal production as a key indicator for evaluating feed utilization efficiency and production benefits.It can be used to assess chicken performance and is frequently used in meat-producing poultry (24).Birds with low FCR are considered as having high feed efficiency.FCR is affected by genetics, health, food, and the environment in which the chickens are raised (24)(25)(26).Neither of the two groups of our study differed significantly in BW, ADG, or ADFI; however, the BW and ADG were higher in the CHM group than that in the CON group.Therefore, supplementation with CHM reduced the FCR and improved the feed utilization efficiency.According to previous studies, the injection of AMT components into poultry muscle significantly alleviated the decrease in feed efficiency of lipopolysaccharide (LPS)-treated broilers (27).Other studies have shown that the addition of AMT polysaccharide and Glycyrrhiza uralensis polysaccharide reduced the FCR and increased ADG of broilers (28); however, the ADG between the two groups was not significantly different in our study.We speculate that AMT and CPO extracts contain numerous beneficial components, including polysaccharides, saponins and so on, which stimulate livestock digestion and absorption, enhance nutrient utilization, and reduce feed waste, thus increasing feed efficiency (27,(29)(30)(31).
Serum biochemical indexes partially reflect metabolism and health state of the body.UA levels in serum reflect both the production and excretion of UA.The present study indicated that CHM increased the level of HDL-C and reduced those of UA, LDH, DAO, and  Frontiers in Veterinary Science 09 frontiersin.orgendotoxin in serum.LDH levels are primarily associated with the extent of cell necrosis and damage to the cell membrane (32).Higher levels of LDH indicate a greater degree of cell necrosis and damage.This increase in LDH levels can be attributed to the anti-inflammatory and antioxidant properties of the extract, as well as its role in regulating cell function.In addition, a positive correlation has been found between endotoxin levels and DAO activity in blood (33).Increased intestinal permeability can compromise intestinal barrier integrity, allowing bacteria and other harmful substances to enter the bloodstream more easily, thereby triggering inflammation and other health issues.Therefore, the addition of CHM can have a positive effect on body metabolism and intestinal barrier function by increasing HDL-C levels and decreasing endotoxin and LDH levels and DAO activity in the blood.
The antioxidant capacity of the body inhibits the production of free radicals and prevents chain reactions of free radicals, thus alleviating oxidative damage to the host.This improvement in antioxidant capacity is beneficial for the health and production performance of broilers (34).T-AOC comprehensively reflects the antioxidant capacity of both enzyme and non-enzymes defense systems (35).Antioxidative enzymes are composed of significant elements such as T-SOD, CAT, and GSH-Px, which have a critical function in eradicating superoxide anions and hydrogen peroxide.These enzymes protect cells and tissues against the harmful effects of oxidative stress (35)(36)(37).Wang, et al. (38) reported that diets supplemented with AMT increased sheep CAT, T-SOD, and T-AOC levels in the small intestinal mucosa and meat tissue, improving their antioxidant capacity.Similarly, adding AMT to quails increased their T-AOC, GSH-Px, and CAT activities (39).Our results showed increased serum and liver T-AOC, SOD, and GSH-Px, and liver CAT levels in the CHM group.This increase may be due to the bioactive compounds present in the CPO and AMT extracts mixtures, including polysaccharides, saponins, and flavonoids, which demonstrate significant antioxidant effects (40).Furthermore, research has revealed that these herbs can modulate the immune system, stimulate cell regeneration and repair, and support normal metabolism and physiological functions, while simultaneously counteracting free radical damage within the body (41)(42)(43).These effects may be because some compounds in CHM are antioxidants that fight free-radical damage in the body and boost antioxidant activity.
An intact intestinal morphology and a healthy intestinal epithelial barrier are important for maintaining animal health, improving immunity, protecting the host from pathogens, and supporting subsequent growth (44)(45)(46).The jejunum serves as the primary site for nutrient absorption, featuring a multitude of villi on the mucosa.The intestinal mucosa has a direct effect on the ability of the host to resist potentially invading pathogens.As villus height increases, both the intestinal surface area and the number of epithelial cells increase, resulting in an enhanced capacity for nutrient absorption (47).In broilers studies, AMT polysaccharide has been shown to increase villus height in the small intestine (48).Our results showed that CHM supplementation increased villus height and the mRNA level of Claudin-1 in the jejunal mucosa.Claudin-1 is an important intestinal tight junction protein that facilitates formation and reinforcement of intercellular junctions, resulting in improved cell epithelial barrier strength and stability (49, 50).These functions were further confirmed by the decreased levels of DAO and endotoxin in the CHM group.The mucosal immune system is an essential component of the immune system (51).In addition, cells of the innate immune system produce important cytokines that contribute to adaptive immunity (52).IL-6 and TNF-α are innate immunity-related cytokines with pro-inflammatory properties that are crucial for host defense, inducing inflammation and triggering apoptosis (53).In contrast, IL-10 is a vital anti-inflammatory cytokine.Elevated levels of IL-10 during inflammation help modulate and balance the inflammatory process, thereby maintaining homeostasis (54).A study demonstrated that CPO induced increased cytokine (IL-2 and IFN-γ) levels, upregulated the expression of the appropriate mRNA in mice, and improved the immune organ index (55).Another study reported that a polysaccharide derived from CPO could potentially have immunomodulatory effects.It was found to enhance the secretion of cytokines (IL-6 and TNF-α) in RAW 264.7 macrophages, without exhibiting any cytotoxic effects (56).Moreover, CPO has been implicated in promoting the production of IL-2, TNF, and IFN in mice upon extraction of pectic polysaccharide (57).Consistent with our present results, we found that the mRNA levels of cytokines IL-6, IL-10, IFN-β, IFN-γ, and TNF-α were elevated in the jejunal mucosa.Therefore, it can be speculated that herbal medicine's active ingredients act as immunostimulants, activating immune cells and promoting proinflammatory mediator secretion, potentially leading to upregulation of cytokines such as IL-6 and TNF-α, which may subsequently upregulate IFN-β and IFN-γ.Concomitantly, upregulation of the anti-inflammatory cytokine IL-10 maintains homeostasis by counteracting the inflammatory process.These findings indicate that CHM may enhance the gut barrier function and promote intestinal health in broilers.
The gut microflora is crucial for regulating intestinal motility, immune homeostasis, and nutrient absorption (58).Furthermore, research has shown that intestinal flora can assist the host in protecting against pathogens and inflammatory bowel diseases, thus enhancing the host's digestive system health (59).Chao 1 is a measure of the community richness.Our findings showed that the addition of CHM reduced microbial richness and the observed number of OUT's, which was different from other studies (28,60).This may be associated with the ability of CHM to eliminate and impede pathogenic diseasecausing microbes in the gut.Furthermore, NMDS analysis indicated that the microbial communities in the CON and CHM groups differed in composition.At the phylum level, our results indicated that Firmicutes and Bacteroides dominated the cecal microflora, which was consistent with the results of previous studies (61,62).The ratio of Firmicutes to Bacteroidetes is an important indicator of gut microbiota health (63), whereas inflammation and gut permeability improve when the ratio of Firmicutes to Bacteroides decreases (64).Studies have suggested that the addition of herbal medicines, such as CPO and AMT, decreases the ratio of Firmicutes to Bacteroidetes in weaned piglets (17), which is consistent with our results.There is evidence that Bacteroidetes and Firmicutes contribute to broiler digestion (65), and Bacteroides genus displays a positive impact on host health and disease resistance (66).In addition, we observed a higher abundance of Bacteroidales_unclassified and lower abundance of Alistipes after CHM supplementation.A study previous found that inhibiting the proportion of Alistipes increased its metabolism (67).In addition, Alistipes, a microbe that produces indole, can disrupt the serotonin balance in the gut through excessive growth (68).Hence, adding AMT and CPO extracts to broiler diets could enhance gut microbiota and health by increasing Bacteroidetes abundance and modulating the Firmicutes to Bacteroides ratio.Spearman's correlation analysis revealed that the cecum microbiota correlated with the FCR, serum differential metabolites, and gene expression in the jejunal mucosa.Bacteroides are frequently associated with the decomposition of polysaccharides, particularly starch and glucan (69).We found that Bacteroidales_ unclassified was positively correlated with immune factors and tight junction protein mRNA expression, whereas it was negatively correlated with the FCR.Similar with our results, previous studies have found a positive relationship between the Bacteroidetes phylum and the plasma level of the pro-inflammatory cytokine TNF-α (70).The genus Bacteroides was linked to the cytokine IL-6 produced by monocytes and maintained the integrity of the epithelial barrier by controlling intraepithelial lymphocytes (from which IL-6 is formed) (71, 72).Bacteroides spp.have been proven to induce macrophages and monocytes to release TNF-α through LPS-mediated pathways (73).It has also been reported that Bacteroides fragilis supplementation enhances the expression of the tightly wound response proteins Claudin-1 using real-time qPCR and immunofluorescence staining (74).In contrast to the genus Bacteroidales_unclassified in the correlation analysis, we found that the genus Alistipes showed a negative correlation with the mRNA expression of immune factors and tight junction protein factor mRNA and a positive correlation with the FCR, which was different from the findings of previous studies (75,76).Given that Alistipes is a subbranch of Bacteroidetes that is relatively recent in terms of pathogenicity, comparative data suggest that Alistipes may cause colon cancer (77).Moreover, Alistipes, a possible pathogen, is implicated in the pathogenesis, which thrives in an inflammatory environment devoid of lipocalin 2, encouraging inflammation and tumor development (78).The present study showed that CHM supplementation increased Bacteroidales_unclassified abundance and decreased Alistipes abundance.Therefore, CHM supplementation improved feed efficiency and increased the mRNA expression of pro-inflammatory cytokines in the jejunal mucosa.Additionally, CHM supplementation decreased the level of endotoxin and activities of DAO and LDH in the serum.These effects may be associated with alterations in the broiler gut microbiota.

Conclusion
Dietary supplementation with 500 mg/kg CHM (AMT and CPO extracts) improved the FCR and antioxidant capacity in the serum and liver and decreased the levels of UA and endotoxin and activity of DAO.Moreover, dietary CHM raised the concentrations of IL-6, IFN-γ, IFN-β, TNF-α, and the anti-inflammatory cytokine IL-10, while it induced alterations in the microbial composition of the cecum in broiler chickens.Spearman correlation analysis identified a correlation between cecal microbiota composition and FCR or serum differential metabolites or genes with significant differences in the jejunal mucosa, which might be attributed to the increased Bacteroidales_unclassified abundance and decreased Alistipes abundance in broilers.Further research is needed to explore the effects and mechanisms of the active ingredients in CHM, primarily AMT and CPO extracts, on animal health.

FIGURE 1
FIGURE 1 Relative abundance of the cecal microbiota.(A) Venn diagram of OTUs; number; (B) NMDS using Bray-curtis distance; (C) Phylum-level taxonomic composition of the cecal microbiota; (D) Genus-level taxonomic composition and relative abundance of the cecal microbiota; (E) The relative abundance of Phylum Firmicutes and Bacteroidetes and genus Bacteroidales_unclassified and Alistipes.(F) Lefse identified the most differential genera between the CON group and the CHM group.CON, control; CHM, Chinese herbal medicine.Significant differences are denoted by asterisks (*p < 0.05; **p < 0.01).

TABLE 1
Composition and nutrient content of experimental diets.

TABLE 2
The primer sequence of the gene.

TABLE 4
Effects of CHM on serum physiological and biochemical indexes of broilers.

TABLE 3
Effects of CHM on growth performance of broilers.ADG, average daily gain; ADFI, average daily feed intake; FCR, feed conversion ratio; CON, control; CHM, Chinese herbal medicine.

TABLE 5
Effects of CHM on the expression levels of immune factors in the serum and jejunal mucosa of broilers.

TABLE 6
Effects of CHM on serum and hepatic antioxidant capacity of broilers.

TABLE 7
Effects of CHM on jejunal morphology and the mRNA expression of mucosa tight junction protein.

TABLE 8
Alpha diversity indexes of cecal microbiota.