Benefits of heat-killed Lactobacillus acidophilus on growth performance, nutrient digestibility, antioxidant status, immunity, and cecal microbiota of rabbits

Introduction Heat-killed probiotics, as a type of inactivated beneficial microorganisms, possess an extended shelf life and broader adaptability compared to their live counterparts. This study aimed to investigate the impact of heat-killed Lactobacillus acidophilus (L. acidophilus, LA) - a deactivated probiotic on the growth performance, digestibility, antioxidant status, immunity and cecal microbiota of rabbits. Methods Two hundred weaned Hyla rabbits were randomly allocated into five equal groups (CON, L200, L400, L600, and L800). Over a 28-day period, the rabbits were fed basal diets supplemented with 0, 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively. Results Results revealed a significant reduction in the feed-to-gain ratio (F/G) in the L600 and L800 groups (p < 0.05). Additionally, the L800 group exhibited significantly higher apparent digestibility of crude fiber (CF) and crude protein (CP) (p < 0.05). Regarding digestive enzyme activities, enhanced trypsin and fibrinase activities were observed in the L600 and L800 groups (p < 0.05). Concerning the regulation of the body’s antioxidant status, the L800 group demonstrated elevated levels of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in both serum and ileal tissue (p < 0.05). In terms of immune capacity modulation, serum tumor necrosis factor-α (TNF-α) levels were significantly lower in the L600 and L800 groups (p < 0.05), while immunoglobulin A (IgA) and immunoglobulin M (IgM) levels were higher (p < 0.05). Additionally, the L800 group exhibited a substantial increase in secretory immunoglobulin A (SIgA) levels in the intestinal mucosa (p < 0.05). In comparison to the CON group, the L800 group exhibited a significant increase in the relative abundance of Phascolarctobacterium and Alistipes in the cecum (p < 0.05). Phascolarctobacterium demonstrated a positive correlation with SIgA (p < 0.05), IgM (p < 0.01), and Glutathione peroxidase (GSH-Px) (p < 0.05), while displaying a negative correlation with TNF-α levels (p < 0.05). Concurrently, Alistipes exhibited positive correlations with IgA (p < 0.05), IgM (p < 0.05), SIgA (p < 0.01), GSH-Px (p < 0.05), SOD (p < 0.05), and T-AOC (p < 0.01), and a negative correlation with TNF-α (p < 0.05). Discussion In conclusion, the dietary incorporation of 600 mg/kg and 800 mg/kg of heat-killed LA positively influenced the growth performance, nutrient digestibility, antioxidant status, immune capacity and cecal microbiota of rabbits. This highlights the potential benefits of utilizing heat-killed probiotics in animal nutrition.


Introduction
Probiotics, defined as living microorganisms with proven health benefits upon proper administration, have been extensively studied for their capacity to enhance host immunity, optimize gut health, and improve overall animal growth performance (1,2).Commonly employed probiotic strains encompass Lactobacillus acidophilus, Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae (3)(4)(5).However, the widespread utilization of live probiotics has unveiled associated risks, including variable colonization patterns, instability of live bacteria, and potential strain transfer across the intestinal barrier (6).Consequently, concerns regarding these risks have led to an increased adoption of paraprobiotics, specifically inactivated probiotics (7).Various methods, including heat, high pressure, and ultraviolet light, have been explored for probiotic deactivation, with heat treatment often preferred.This choice is due to its effectiveness, cost-efficiency, and ability to preserve probiotic integrity, ensuring prolonged functionality and stability.Heat treatment stands as a widely adopted approach for preserving probiotic attributes across different contexts (8).Recent studies have substantiated that non-viable bacteria, notably inactivated probiotics, can confer health benefits akin to their living counterparts.These benefits encompass the modulation of immunity, pathogen suppression, and the enhancement of gut microbes to uphold overall animal health.Notably, these functional attributes of inactivated probiotics are attributed to their release of lipophosphatidic acid (LTA), peptidoglycan, and extracellular polysaccharide (EPS) components (9,10).Additionally, the ease of storage and transportation of inactivated probiotics, compared to live bacteria, further underscores their practical advantages (7,11,12).
The weaning period poses a critical juncture for rabbits, characterized by an underdeveloped digestive tract and exposure to multiple stressors, including dietary transitions and relocation to new enclosures, potentially impacting their overall growth and developmental trajectories (13).Post-weaning, the abrupt cessation of breast milk antigen supply results in a rapid decline in intestinal immunity (14).Consequently, rabbits become more susceptible to environmental variations, eliciting stress responses that can manifest as diarrhea and, in severe cases, culminate in death (15).
Prior research has demonstrated the positive impact of dietary supplementation with alive LA on enhancing the dietary conversion rate, promoting growth performance, and augmenting beneficial intestinal flora in rabbits (4).Moreover, LA has been shown to fortify immunity, improve the integrity of the intestinal barrier, diminish the occurrence of diarrhea, and reduce mortality rates (4,5,16).Despite these established benefits, there remains a scarcity of literature addressing the utilization of heat-killed LA in rabbit production.This study aims to bridge this knowledge gap by investigating the repercussions of incorporating heat-killed LA into rabbit diets.The investigation also encompasses an in-depth analysis of its effects on growth performance, digestibility, digestive enzymes, antioxidant status, and immune capacity in rabbits.The results of this research will provide valuable scientific insights and provide a powerful reference for the rational application of heat-killed LA in rabbit production.

Animals and management
A total of 200 healthy Hyla meat rabbits, uniform in weight at 35 days of age, were selected and randomly allocated into 5 groups, each comprising 8 replicates, with 5 rabbits in each replicate.Rabbits in the control group (CON) were exclusively fed a basal diet without any supplementation.In contrast, rabbits in the 4 treated groups, namely L 200 , L 400 , L 600 , and L 800 , received basal diets enriched with 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively, for a duration of 28 days.The heat-killed LA was sourced from Biosource Biotechnology Co., Ltd.(Shenzhen, China) and contained 1 × 10 9 cfu/g of LA in a dried product stored at room temperature (the main ingredients in this product include inactivated Lactobacillus acidophilus and silica).The formulation of the base diet adhered to the nutritional recommendations for rabbits as outlined by De Blas (17).The composition and nutritional levels of the base diet are detailed in Table 1.Both feeding and digestion experiments were carried out at the rabbit farm located within the Animal Husbandry Teaching Base of Hebei Agricultural University.Prior to the commencement of the experiments, meticulous cleaning and disinfection procedures were implemented for all rabbit cages.The feeding trial spanned a duration of 35 days, encompassing a pre-feeding phase of 7 days and a subsequent standard trial period of 28 days.Throughout the trial, all rabbits were afforded ad libitum access to both feed and water.

Growth performance
The composition and nutritional levels of the base diet are detailed in Table 1.Initial body weight (IBW) and final body weight (FBW) were recorded at the beginning and at the end of the experiment (70 days of age) by weighing on an empty stomach.Record the feeding amount and remaining feed amount of each group of rabbits, calculate the average daily feed intake (ADFI), average daily weight gain (ADG) and feed-to-weight ratio (F/G).ADFI, ADG and F/G were calculated as follows: ADFI = total feed intake/experimental days; ADG = (FBW − IBW)/experimental days; F/G = ADFI/ADG.
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Nutrient digestibility
At the end of the experiment, a 7 days digestibility test was conducted.During the trial, feces from each replicate were collected, weighed, and frozen at −20°C to be prepared for chemical analysis.Crude protein (CP), crude fat (EE), crude fiber (CF), acid detergent fiber (ADF), and neutral detergent fiber (NDF) and other nutrients in feed and feces were determined using the Association of Official Analytical Chemists (AOAC) method (18).Calculation of the digestibility of rabbits according to the total manure collection method.The digestibility of nutrients was calculated according to the following formula: where t is the nutrient intake [g] during collection and f is the amount of nutrients excreted in the feces [g].

Samples collection
Upon completion of the experimental period, rabbits underwent a 12 h fasting period.Subsequently, one rabbit was randomly chosen from each replicate, and a blood volume of 10 mL was collected from the marginal ear vein.Blood samples were collected in vacuum containers and subjected to centrifugation at 3,000 × g for 10 min at 4°C to isolate the serum.The serum samples were preserved at −20°C until further analysis.Following blood collection, rabbits were humanely euthanized using the pentobarbital method.Tissue samples from the jejunum, ileum, and cecum were obtained using meticulously sterilized surgical instruments.The jejunum, ileum, and cecal chyme were carefully stored in sterile lyophilized test tubes.The 5 cm jejunum and ileum segments were placed on an ice tray and the mucosa was gently scraped off with a slide (the mucosa volume was 3-3.5 mL).The scraped mucous membranes were stored in sterile lyophilized test tubes at −80°C for subsequent analysis.

Immune capacity
The quantification of IgA, IgG, IgM, IFN-γ, TNF-α, IL-4, and IL-6 levels was conducted using enzyme-linked immunosorbent assay (ELISA).Similarly, intestinal mucosal Secretory SIgA and MUC2 were also assessed via ELISA kits.All ELISA kits utilized in the study were procured from Beijing Borealis Technology Co (Beijing, China), and specific procedural guidelines outlined in the respective kit instructions were followed for accurate implementation.

Digestive enzyme activity and antioxidant status
The α-amylase, trypsin, lipase activity, and cellulase activity of intestinal contents, as well as the total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and malondialdehyde (MDA) content in serum, jejunal and ileal tissues were measured using ELISA kits (Beijing Borui Long Term Technology Co (Beijing, China)).

16S rRNA gene sequencing and analysis
The cecum contents, approximately 5 mL, were collected in sterile cryopreservation tubes and stored at −80°C.Total genomic DNA was extracted from cecal digesta using the OMEGA Soil DNA Kit (M5635-02; Omega Bio-Tek, Norcross, GA, United States) following the manufacturer's instructions.The quantity and quality of the extracted DNA were assessed using a NanoDrop NC2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States) and agarose gel electrophoresis, respectively.PCR amplification of the V3-V4 region of the bacterial 16S rRNA gene was performed using forward primer 338F (5′-ACTCCTACGGGAGGCAGCA-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′).The amplification procedure consisted of: 98°C for 5 min, followed by 25 cycles of 98°C for 30 s, 53°C for 30 s, and 72°C for 45 s, with a final extension at 72°C for 5 min.The amplicons were purified with Vazyme VAHTSTM DNA Clean Beads (Vazyme, Nanjing, China) and quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA, United States).After individual quantification, the amplicons were pooled in equal amounts, and paired-end sequenced (2 × 250 bp) at Shanghai Personal Biotechnology Co., Ltd.(Shanghai, China) on the Illumina NovaSeq Platform using the NovaSeq 6,000 SP Reagent Kit (500 cycles).To estimate microbial diversity in individual samples, alpha diversity indices, such as the Chao1 richness estimator, Shannon diversity index, Simpson index, and Goods_coverage.Observed_species at the amplicon sequence variant (ASV) level were calculated using the ASV table in QIIME2.

Date analysis
Statistical data analyses were performed using Excel 2020 and SPSS 26.0 software.One-way analysis of variance (ANOVA) was used to test for significant differences between groups of data, while Duncan's method was used for multiple comparisons.p-value <0.05 indicated significant differences between groups.

Growth performance
The supplementation of heat-killed LA in the diet did not yield statistically significant differences (p > 0.05) in FBW, ADF, and ADG among rabbits, as indicated in Table 2. Nevertheless, noteworthy reductions were observed in the Feed-to-Gain ratio (F/G) within the L 600 and L 800 groups, and these reductions were statistically significant (p < 0.05).

Nutrient digestibility
As presented in Table 3, the digestibility of CP exhibited a notable increase in the L 800 group, achieving statistical significance (p < 0.05).Moreover, the digestibility of CF and NDF in the L 600 and L 800 groups demonstrated a significant improvement when compared to the CON group (p < 0.05).Conversely, the digestibility of GE and other nutrients, including DM, ADF, EE, Ash, Ca, and P, did not manifest significant differences in rabbits when compared to the CON group (p > 0.05).

Digestive enzyme activity
The effects of the different treatments on intestinal digestive enzyme levels are presented in Figure 1.Compared with CON group, the activities of jejunum and ileum trypsin and cecum cellulase in L 600 and L 800 groups were significantly increased (p < 0.05) (Figures 1B,E,G), and the jejunum lipase activity in L 800 group was also significantly higher (p < 0.05) (Figure 1C).Compared with CON group, α-amylase activity in jejunum and ileum and lipase in ileum of rabbits in experimental groups were not significantly different (p > 0.05).

Antioxidant indexes
In comparison to the CON group, the SOD activity in the serum and intestine tissue of the L 600 and L 800 groups exhibited a significant increase (p < 0.05) (Figures 2B,F,J).Additionally, the serum T-AOC activity was significantly elevated in the L 800 group (p < 0.05, Figure 2D).Furthermore, the T-AOC activity in the ileum of rabbits in the L 600 and L 800 groups demonstrated a noteworthy increase in comparison to the CON group (p < 0.05) (Figure 2L).However, there were no statistically significant differences observed in MDA content and GSH-Px activity within the serum, jejunum, and ileum between the experimental groups and the CON group (p > 0.05).

Serum immune capacity
In Figure 3, it was shown that serum TNF-α levels were significantly reduced in the L 600 and L 800 groups compared with the CON group (p < 0.05) (Figure 3D).The serum IgA levels in the L 800 group were significantly increased (p < 0.05) (Figure 3E).In addition, serum IgM levels were significantly higher in the L 600 and L 800 groups compared with the CON group (p < 0.05) (Figure 3G).And IFN-γ, IL-4, IL-6, and IgG levels were not significantly different compared with the CON group (p > 0.05).

Intestinal mucosal immune capacity
The findings presented in Figure 4 indicate a substantial increase in SigA content in the jejunal mucosa of rabbits within the L 800 group compared to the CON group (p < 0.05) (Figure 4A).Similarly, ileum mucosa was significantly increased in the L 600 and L 800 groups (Figure 4C).However, there were no significant differences observed in mucosal MUC2 content in the jejunum and ileum between the experimental groups and the CON group (p > 0.05).

Cecum microflora
The operational taxonomic unit (OTU) analysis revealed varying numbers of identified OTUs across the experimental groups, with  5.The microbial diversity within an individual sample was assessed by the Chao1, Goods_ coverage, Shannon, Simpson indices, and Observed_species but no significant differences between all groups were observed (Figure 6).Principal component analysis of OTUs was performed to evaluate the similarities and differences between the control group and the experimental group (Figure 7), and the results showed that the cecal microbiota of CON group was significantly separated from that of L 400 , L 600 , and L 800 groups.Taxonomic profiling indicated that Firmicutes and Bacteroidetes accounted for most of the intestinal bacteria of rabbits (Figure 8).At the phylum level, there were no significant differences in the relative bacterial abundance among all groups (p > 0.05) (Table 4).The relative abundance of the 20 dominant genera in each group at the genus level was also analyzed.Ruminococcus and Oscillospira were found to be the main genera in the 4 treatment groups (Figure 9).As shown in Table 5, compared with the CON group, the relative abundance of Phascolarctobacterium in L 600 and L 800 groups was significantly increased, Subdoligranulum in L 200 group was significantly increased, and Dehalobacterium in L 400 , L 600 , and L 800 groups was significantly decreased.The relative abundance of Alistipes in group L 800 was significantly increased (p < 0.05).

Association of cecum microflora with immunity and antioxidant capacity
In order to further understand the potential relationship between gut microbes and immunity and antioxidant capacity, Spearman correlation analysis was used to analyze the relationship.As shown in Figure 10

Discussion
The weaning stage poses a critical period for rabbits, susceptible to external stimuli such as dietary changes and environmental shifts, potentially leading to appetite loss, and even an increase in mortality and diarrhea rates (19).Zhu et al. (20) observed that the supplementation of 500 mg/kg of inactivated complex probiotics in the diet positively influenced cecum microbiota, consequently improving diet conversion and growth performance in yellow-finned   21) demonstrated enhanced feed efficiency and ADG in pigs through the utilization of 20 mg/kg of heat-killed L. plantarum.Consistent with prior studies, the current research underscores that the addition of 600 mg/kg and 800 mg/kg of heat-killed LA significantly reduces F/G and enhances the growth performance of rabbits.This improvement in feed efficiency correlates with the observed elevation in the activity of digestive enzymes (lipase, protease, cellulase) within the intestinal tract of rabbits (22).Notably, the potential role of heat-killed LA in regulating gut microflora and diminishing the incidence of diarrhea aligns with the findings of Moal et al., who observed a reduction in harmful bacterial adhesion in the intestine through the use of inactivated probiotics (23), resulting in a decreased frequency of diarrhea in piglets (24).In summary, we hypothesize that the improved growth performance may also be related to heat-killed LA by modulating gut microflora and mitigating the occurrence of diarrhea.
Apparent digestibility serves as a key indicator of an animal's proficiency in nutrient digestion within the diet, and this is intricately linked to digestive enzyme activity, contributing positively to growth performance (25,26).Mammalian food nutrients primarily undergo digestion orchestrated by various digestive enzymes, with a minor portion being subject to digestion by intestinal bacteria (27,28).Palamidi et al. (29) reported an elevation in lipase activity and  improved digestibility in broilers when fed heat-killed complex probiotics.Similarly, Dawood et al. (30) observed a significant increase in amylase, protease, and lipase activities following the inactivation of L. plantarum, leading to enhanced nutrient digestion.In concordance with these findings, the current study demonstrated a substantial increase in trypsin, lipase, and cellulase activities upon supplementing diets with 600 mg/kg and 800 mg/kg of heat-killed LA.This trend aligned with the concurrent increase in the apparent digestibility of CP and CF in rabbits.Furthermore, the inactivation of LA was found to enhance the intestinal microbial composition, favoring an increased relative abundance of beneficial bacteria such as Lactobacillus and Bacillus in the intestine (31,32).This augmentation in beneficial microbial communities within the gut can potentiate their ability to induce the production of endogenous enzymes (33,34).Consequently, the inactivation of LA appears to contribute to the enhancement of digestive enzyme activity, ultimately improving digestibility and, consequently, the growth performance of rabbits.Oxidation reactions, yielding ROS, are constant processes within organisms.Under normal physiological conditions, animal cells maintain a delicate equilibrium between ROS production and clearance (35).Excessive accumulation of free radicals poses a significant threat to tissues, leading to intestinal damage, nutrient malabsorption, and severe diarrhea (36,37).Antioxidant defense enzymes, including SOD and GSH-Px, play pivotal roles in  Venn diagram showing common and unique operational taxonomic units (OTUs) in the cecal digest of rabbits.According to the feature abundance table, the number of features in each group was calculated, and the number of common and unique features in each group was visualized by Venn diagram, if the feature existed in only one group, it was unique to that group.Principal co-ordinates analysis diagram of cecum microflora composition.Principal Coordinate Analysis (PCoA) is based on a sample distance matrix for ranking.The PCoA analysis allows the difference among the groups to be observed.The different colours in the results represent the different groupings and the closer the distance, the more similar the structure of the microbial composition and the less difference.
Relative abundance of cecum microbiota at phylum level.counteracting oxidative stress SOD, functioning as the primary defense against free radicals, not only exhibits superb antioxidant effects but also inhibits the synthesis and release of inflammatory factors (39).T-AOC, encompassing both enzymatic and non-enzymatic antioxidant activities, provides a comprehensive assessment of the organism's antioxidant status (40).Previous research, such as Liu et al. (41) and Dawood et al. (30) has highlighted the antioxidant properties of heat-killed LA and heat-killed L. plantarum, respectively.In this experiment, supplementation with heat-killed LA demonstrated a notable increase in SOD activity and T-AOC capacity in rabbits, with the most pronounced effects observed at supplementation doses of 600 mg/kg and 800 mg/kg.This Relative abundance of cecum microbiota at genus level.augmentation in antioxidant-related enzyme activity may be attributed to the ability of heat-killed Lactobacillus activate the Nrf2 signaling pathway (42,43).Activation of the Nrf2 signaling pathway triggers the transcription and translation of downstream antioxidant genes (such as SOD and T-AOC), bolstering antioxidant status in response to environmental stress and sustaining normal intestinal function (44).Immunoglobulin (IgA, IgM, and SigA etc.) is one of the components of animal immune system and an important parameter reflecting the immune capacity of animal body (45).IgA plays a crucial role in protecting the mucosal surface by preventing the entry, binding, and colonization of toxins and pathogens (46).IgM is the immunoglobulin with the strongest initial immune effect, and is an important barrier against pathogenic invasion (47).In this study, supplementation with heat-killed LA significantly increased the levels of IgA and IgM in serum, which reflected that heat-killed LA could improve the immune capacity of rabbits.As the main immune defense line of intestinal mucosa, SigA has the function of neutralizing pathogens and bacterial exotoxins in intestinal mucosa and maintaining the stability of intestinal flora (48).Danladi et al. found that supplementation of feed with inactivated Lactobacillus plantarum significantly increased the intestinal SigA content of broiler chickens and improved immunity (49).Consistent with the results of previous studies, we found that feed supplementation with 600 mg/kg and 800 mg/kg of heat-killed LA significantly enhanced the secretion of intestinal SigA, protected the intestinal immune barrier, and reduced the occurrence of intestinal diseases.TNF-α is a pro-inflammatory cytokine produced mainly by macrophages and monocytes, which is involved in the inflammatory response, and when its level is too high, it increases intestinal permeability and causes an enteritis response (50).It was found that LTA isolated from Lactobacillus can block the phosphorylation of NF-κB and the phosphorylation degradation of IkB, thereby inhibiting the expression of TNF-α (51).In this experiment, we found that supplementation with heat-killed LA reduced the serum levels of TNF-α.This may be related to the blocking of NF-κB phosphorylation by heat-killed LA.It was also shown that heat inactivation of LA does not cause an inflammatory response in the meat rabbit organism and has no adverse effect on its health.
There are a large number of microorganisms in the intestinal tract of animals, and their species composition and activity play an important role in the immune and inflammatory responses of animals (52, 53).Therefore, the balance of intestinal flora is related to the health of animals.Under normal conditions, the composition of the Heatmap of the correlation between cecum microbiota and immunity.The correlations were significant (*p < 0.05 and **p < 0.01), positive in green and negative in brown.
intestinal flora is relatively but at the same time it can be disturbed by external factors (54).the current study, probiotic supplements or probiotic products were found to be one of the effective ways to maintain a healthy balance of gut microbiota (55)(56)(57)(58).In addition, species-rich communities enhance intestinal microecological stability and reduce susceptibility to bacterial invasion and intestinal inflammation (59).In this study, dietary heat-killed LA can regulate the cecal microflora structure of rabbits and increase the relative abundance of Phascolarctobacterium and Alistipes.Phascolarctobacterium and Alistipes are probiotics that promote intestinal health.Their main function is to produce short chain fatty acid (SCFAs), which play a vital role in maintaining nutrient metabolism and microbial homeostasis (60, 61).Acetic acid is not only the most abundant SCFAs in the gut, but also inhibits the NF-κB signaling pathway by down-regulating the expression of TLR gene, thereby reducing the content of TNF-α in the body and reducing the inflammatory response (62).In addition, SCFAs can also accelerate the activation, differentiation and antibody production of B cells by promoting the synthesis of acetyl-CoA, 5'-adenosine triphosphate and fatty acids in B cells, thus promoting the secretion of SigA, IgA, and IgM (63,64).SCFAs can also act as an activator of Keap1-Nrf2 signaling pathway (65,66), and the activation of Nrf2 is positively correlated with SOD, GSH-Px, and T-AOC (44, 67).The above results showed that heat-killed LA can promote the proliferation of Phascolarctobacterium and Alistipes, thereby reducing serum TNF-α level, and increasing jejunum SigA, serum IgM, serum IgA, ileum GSH-Px, jejunum GSH-Px, ileum SOD and serum T-AOC levels, which may be due to the regulation of SCFAs by Phascolarctobacterium and Alistipes.These results provide a theoretical basis for understanding heat-killed LA-mediated microbial promotion of immunity and antioxidant capacity in rabbits.However, further microbiome-based studies are needed to elucidate the mechanisms by which Phascolarctobacterium and Alistipes promote immunity and antioxidant capacity in rabbits.

Conclusion
In conclusion, the incorporation of heat-killed LA into the diet demonstrates a capacity to enhance intestinal digestive enzyme FIGURE 11 Heatmap of the correlation between cecum microbiota and antioxidant capacity.The correlations were significant (*p < 0.05 and **p < 0.01), positive in green and negative in brown.activity, bolster antioxidant status, modulate immune capacity, increase the relative abundance of beneficial bacteria in the cecum and overall organismal health in rabbits.This ultimately translates into improved growth performance.Upon comprehensive analysis, the recommended additive levels of heat-killed LA in rabbit diets appear to be 600 and 800 mg/kg.

FIGURE 6
FIGURE 6Alpha diversity indices of cecum microbiota.Chao 1 and Observed_otus indices mainly reflect the richness of the species in the sample; Goods_ coverage reflects the low abundance feature coverage of the sample; Simpson and Shannon reflect the richness and evenness of the species together.

TABLE 1
Composition and nutrient levels of the basal diet (air-dry basis, %).

TABLE 2
Effect of heat-killed Lactobacillus acidophilus on the growth performance of rabbits.,085, 4,421, 3,890, and 4,446 OTUs in the CON, L 200 , L 400 , L 600 , and L 800 groups, respectively.Notably, 1,116 OTUs were shared among the four groups, as illustrated in Figure IBW, initial body weight; FBW, final body weight; ADFI, average daily feed intake; ADG, average daily gain; F/G, ratio of feed and gain.Data are means for 8 replicates of 5 rabbits per replicate.SEM, total standard error of the means (n = 8).a,b Means with different superscripts in the same row are significantly different (p < 0.05).

TABLE 3
Effect of heat-killed Lactobacillus acidophilus on apparent digestibility (%) of energy and nutrients in rabbits.
DM, dry matter; GE, gross energy; CP, crude protein; EE, ether extract; CF, crude fiber; NDF, neutral detergent fiber; ADF, acid detergent fiber; Ca, calcium; P, total phosphorus.SEM, total standard error of the means (n = 8).a,b,c Means with different superscripts in the same row are significantly different (p < 0.05).

TABLE 4
Relative abundance of caecal microbiota at phylum level., total standard error of the means (n = 4).a,b,c Means with different superscripts in the same row are significantly different (p < 0.05). SEM

TABLE 5
Relative abundance of caecal microbiota at genus level.