The regulatory mechanism of garlic skin improving the growth performance of fattening sheep through metabolism and immunity

1 Introduction

Garlic, as an herbaceous plant, is a natural broad-spectrum plant-based antibiotic due to its unique organic sulfur compounds, which have functions such as antibacterial, anti-inflammatory, antioxidant, and immune regulation (1, 2). Additionally, garlic is rich in vitamins and amino acids, making it a good nutritional supplement (3, 4). Garlic skin (GAS), as a by-product of garlic processing, has similar detoxification, sterilization, disease prevention, and treatment effects as garlic (5). In addition, GAS also contains abundant vitamins and minerals, which are of great significance for enhancing animal physical fitness and improving production performance (6). Research has found that GAS contains allicin, which can increase gastric secretion and gastrointestinal peristalsis, stimulate appetite and promote digestion, kill various pathogenic microorganisms such as Staphylococcus, Bacillus, and Salmonella, prevent and treat diseases such as enteritis, dysentery, and coccidiosis, thereby improving livestock production performance and reducing production costs (7–10). In addition, allicin has antioxidant ability, which can inhibit the production of ROS, reduce animal energy consumption, and improve production efficiency (11, 12).

Healthy breeding is the current direction of animal husbandry development, which not only solves the environmental pollution problem of animal husbandry, but also improves the health level of animals, and promotes the healthy and sustainable development of modern animal husbandry (13, 14). In the development of traditional animal husbandry, antibiotics or related drugs are widely used to reduce the occurrence of animal diseases and improve animal production performance (15). However, the abuse of antibiotics, such as over dosage and over range use, has posed a serious threat to quality of animal products and food safety (16). With the reduction and prohibition of antibiotic use in animal husbandry, research on antibiotic substitutes has become an important research direction in modern animal husbandry (17, 18). Therefore, the application of GAS as a substitute for antibiotics in the sheep farming industry can reduce economic costs and the threat caused by drug-resistant bacteria, which is of great significance for achieving healthy sheep farming. However, there is still limited research on the application of GAS in healthy breeding of meat sheep. This study added GAS to the feed of fattening sheep to explain the regulatory mechanism of GAS on the growth performance of fattening sheep from multiple aspects such as rumen and fecal microbiota, serum and urine metabolism, and rumen epithelial cell transcriptomics. This provides a theoretical basis for the promotion and utilization of GAS as a substitute for antibiotic additives in animal feed, improvinging animal growth performance.

2 Materials and methods

2.1 Experimental animals and experimental design

This experiment was conducted on the basis of adding 80 g/kg dry matter (DM) garlic skin (GAS) to the feed of fattening sheep to improve their growth performance (19). Twelve healthy 3.5 month old Hu sheep (Chinese native sheep breeds) with similar weight (23.0 ± 2.3 kg) were selected and randomly divided into two groups, with 6 sheep in each group. The control group (CON) was fed a basal diet, while the GAS group (GAS) was fed a basal diet supplemented with 80 g/kg DM GAS, with free eating and drinking water. The experimental period was 10 weeks, with a pre-feeding period of 2 weeks and a regular feeding period of 8 weeks. Before the experiment began, the enclosure was disinfected and routine epidemic prevention measures such as deworming were taken on the experimental sheep. During the experiment, the sheep shed maintained ventilation, suitable temperature, and humidity. During the experiment, the nutritional composition indicators of the CON group basal diet and the GAS group 8% GAS diet for fattening sheep are shown in Table 1 (19). The experimental design and workflow are shown in the Figure 1.

Table 1

Table 1. Main components of the diet for fattening lamb during the experimental period.

Figure 1

Figure 1. Experimental design and workflow of feeding fattening lamb with garlic skin diet. Including fecal microbiome, serum and urine metabolome, and transcriptome sequencing of rumen epithelial cells. Twelve Hu lamb were randomly assigned to a basal diet (CON) or a basal diet supplemented with 80 g/kg garlic skin DM (GAS).

2.2 Collection and determination of samples

Before feeding on the last morning of the experiment, feces, serum, and urine samples were collected from fattening sheep. Feces were collected using rectal sampling method and placed in a 2 mL cryotube in liquid nitrogen for fecal microbiological analysis. Blood was collected from the fattening sheep using an empty stomach jugular vein blood collection method, which was left at room temperature for 2 h, centrifuged at 3500 r/min for 15 min, and the supernatant was serum, after absorbing the serum, it was divided into 2 mL cryotubes and stored in liquid nitrogen. Fasting fattening sheep blood was collected using the jugular vein blood collection method. It was left to stand at room temperature for 2 h, centrifuged at 3500 r/min for 15 min, and the supernatant serum was absorbed. The serum was then loaded into a 2 mL cryotube and placed in liquid nitrogen, with a portion of the serum used for serum antioxidant index analysis, including total antioxidant capacity (T-AOC) (A015-1-2, Nanjing Institute of Biotechnology), superoxide dismutase (SOD) (A001-3-2, Nanjing Institute of Biotechnology) Glutathione peroxidase (GSH-Px) (A005-1-2, Nanjing Jiancheng Institute of Biotechnology) and catalase (CAT) activity (A007-1-1, Nanjing Jiancheng Institute of Biotechnology), as well as malondialdehyde (MDA) content (A003-1-2, Nanjing Jiancheng Institute of Biotechnology). The remaining amount was used for serum metabolomics analysis. Fresh urine from 12 fattening sheep was collected and stored in a 2 mL cryotube in liquid nitrogen for metabolomics analysis of urine.

At the end of the experiment, fasting fattening sheep were slaughtered, and rumen epithelial tissue was collected. The tissue was rinsed with physiological saline and stored in a 2 mL cryotube and liquid nitrogen for transcriptome sequencing. The collected gastrointestinal tissue was fixed with 4% paraformaldehyde, and histological sections were made using HE staining method. Neutral gum was used for sealing and microscopy observation. All experimental operations were carried out wearing disposable sterile masks and gloves to avoid contamination.

2.3 16S rRNA sequencing of fecal microorganisms

The fecal samples were thawed at 4°C, and the genomic DNA of the fecal samples was extracted using E.Z.N.A. fecal DNA kit (Omega Bio tek, Norcross, GA, United States). The DNA samples were measured at OD260/OD280 ratio, and the DNA integrity was detected by 1% agarose gel electrophoresis. Using DNA as a template, the PCR amplification was performed by using specific primers for the variable region of 16S rRNA V3-V4. After passing the PCR product detection and purification, it was sent to Shanghai Shenggong Biotechnology Services Co., Ltd. for high-throughput 16S 156 rRNA sequencing of fecal microorganisms based on the Illumina Miseq platform.

2.4 Metabolomics detection of serum and urine

Serum and urine samples were sent to Shanghai Baiqu Biotechnology Co., Ltd. for metabolomics testing and analysis. The entire experimental process includes sample processing, non-targeted metabolomics testing, and analysis, all completed by Shanghai Baiqu Biotechnology Co., Ltd. A metabolomics analysis was conducted on frozen serum and urine samples of fattening sheep using Ultra-high-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The obtained raw data was processed and analyzed. When the p-value of Student’s t-test was less than 0.05 and the Variable Importance in the Projection (VIP) of the first principal component of the OPLS-DA model was greater than 1, it was considered that there was a significant difference in metabolites. Subsequently, metabolic pathway analysis of differential metabolites was conducted.

2.5 Transcriptomics analysis

The frozen fattening sheep rumen epithelial tissue samples were transported to Shanghai Sangong Biotechnology Co., Ltd. with dry ice for transcriptome sequencing. First, the total RNA was extracted from the samples by the Trizol method for purity and concentration detection, and the integrity of RNA was detected by 1% agarose gel electrophoresis. The qualified samples were sequenced using Illumina HiSeqTM high-throughput sequencing platform, and the sequencing data were processed and analyzed. Using DESeq2 for differential expression gene analysis, the screening conditions were: p-value<0.05 and fold of difference | Fold Change | > 2. Annotate the differential gene expression function and explore the biological functions involved in differential expression genes (DEGs), mainly including biological processes, cellular components, and molecular functions. Cluster Profiler was used for functional enrichment analysis, and when p-value<0.05, this function was enriched.

2.6 Statistical analysis

All experimental data were organized using Excel, and the SPSSAU data analysis platform¹ was used for the normal distribution test of the data. The SPSS software independent sample T-test was used for statistical analysis. When p < 0.05, the difference was significant, and p < 0.01, the difference was extremely significant. Each group of experiments had 6 replicates.

3 Results

3.1 Growth performance

Previous studies have found that adding 8% GAS to the diet could significantly increase the average daily feed intake and weight gain of fattening sheep (p < 0.05), and the feed conversion rate (average daily weight gain/DM intake) had an upward trend (0.05 < p < 0.10), indicating that GAS could improve the growth performance of fattening sheep (19). Based on the findings of this study, further analysis was conducted on fecal microbiota, urine and serum metabolomics, as well as rumen epithelial cell metabolomics. The mechanism of the effect of adding GAS to the diet on the growth performance of fattening sheep was thoroughly analyzed.

3.2 Fecal microbiota

Microorganisms that coevolve in the gut of animal hosts can form a complex microecosystem within the animal organism, known as gut microbiota (20). The gut microbiota of livestock and poultry mainly consists of Firmicutes and Bacteroidetes, followed by Proteobacteria, Actinobacteria, and Fusobacteria. In the bar chart of the genus level community structure distribution of samples in the GAS and CON groups, the community structure of fecal microorganisms at the genus level was presented (Figure 2). Sporobacter, Bacteroides, Alistipes, Treponema, Ruminococcus and other bacterial genera were detected in both GAS and CON groups. Four significant differences were identified between the GAS group and the CON group, namely Metanobrevibater, Akkermansia, Parasutterella, and Guggenheimella (Table 2). Compared to the CON group, the GAS group significantly upregulated the genus Metanobrevibate; The significantly downregulated ones were Akkermansia, Parasutterella, and Guggenheimella.

Figure 2

Figure 2. Distribution characteristics of fecal microbiota in the GAS group.

Table 2

Table 2. Change characteristics of the main microbial communities in the feces of the GAS diet group.

3.3 Serum antioxidant and metabolomics

The level of serum antioxidant capacity reflects the level of antioxidant stress in the body, which is closely related to the health level of the body and is of great significance for the growth and development of fattening sheep. Serum antioxidant indicators were tested on 12 fattening sheep in the GAS and CON groups, including glutathione peroxidase (GSH-Px), malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) (Table 3). Compared with the CON group, the GAS group significantly increased serum GSH-Px and significantly decreased MDA in fattening lamb (p < 0.05); SOD and T-AOC were slightly higher than those in the CON group.

Table 3

Table 3. Effect of GAS diet on serum antioxidant capacity of fattening lamb.

The metabolites in serum come from various metabolic pathways of the body. Studying metabolites in serum can provide a comprehensive understanding of the body’s metabolism and evaluate its physiological and pathological status. We have screened a total of 166 differential metabolites, including 54 anionic modes and 112 cationic modes (Supplementary Table S1; Figure 3). There were significant differences in serum differential metabolites between the GAS group and the CON group in fattening sheep. Compared to the CON group, the GAS group significantly upregulated a total of 45 differential metabolites, such as Pyrocatechol, Cortisone acetate, Salicyluric acid, Verapamil, Propazine, and Miglitol, A total of 121 types of significant downregulation were observed, such as 2 ‘- O-methylinosine, Hexacosanoic acid, L-Citrulline, Acetohydroxamic acid, Cytosine, and Glycerophosphocholine.

Figure 3

Figure 3. Hierarchical cluster analysis of Serum metabolites in Fattening Lamb. (A) Hierarchical cluster analysis of GAS group serum in cationic mode. (B) Hierarchical cluster analysis of GAS group serum in anionic mode.

Further metabolic pathway analysis was conducted on differential metabolites to identify the key metabolic pathways with the highest correlation with metabolic differences. We screened 42 key pathways with the highest differential correlation with metabolites between the GAS and CON groups’ serum (Supplementary Table S2; Figure 4). The key pathways with the highest differential correlation with metabolites between the GAS and CON groups were Taurine and hydroxylamine metabolism, D-glutamine and D-glutamite metabolism, Arginine and proline metabolism, Pyrimidine metabolism, Biosynthesis of unsaturated fatty acids, and Primary bill acid biosynthesis.

Figure 4

Figure 4. Pathway analysis of serum metabolomics. (A) Pathway analysis of GAS group serum in cationic mode. (B) Pathway analysis of GAS group serum under anionic mode.

3.4 Urine metabolomics

Metabolomics analysis was conducted on the urine of fattening sheep in the GAS and CON groups, identifying 68 differential metabolites in the urine, including 27 anionic modes and 41 cationic modes (Supplementary Table S3; Figure 5). The differential metabolites mainly included amino acids, fatty acids, purine pyrimidines, and organic acids. These differential metabolites were mainly divided into two categories: one was 58 significantly downregulated differential metabolites compared to the CON group, mainly including oxypurinol, 2 ‘- Deoxyuridine, Shikimate, Gamma aminobutyric acid, Indoleacrylic acid, and Dimethylbenzimidazole; The other type was significantly upregulated by 10 metabolic markers, such as Citric acid, 3-Aminopropoanesulfonic acid, L-Cystine, 3-Methodybenzoicacid, 1,2-Diacetylhydrazine, and Photinus luciferin.

Figure 5

Figure 5. Hierarchical cluster analysis of urine metabolites in fattening lamb. (A) Hierarchical cluster analysis of GAS group urine in cationic mode. (B) Hierarchical. Cluster analysis of GAS group urine in anionic mode.

Enrichment analysis and topological analysis were conducted on the pathways involved in differential metabolites, and 23 metabolic pathways related to differential metabolites in urine were identified (Supplementary Table S4; Figure 6). Metabolic pathway analysis showed that Cysteine and methionine metabolism, Pyrimidine metabolism, Methane metabolism, Riboflavin metabolism, Tryptophan metabolism and Valine, leucine and isoleucine biosynthesis, etc.

Figure 6

Figure 6. Pathway analysis of urine metabolomics. (A) Pathway analysis of MHB group urine in cationic mode. (B) Pathway analysis of MHB group urine under anionic mode.

3.5 CircRNA bioinformatics

CircRNA (Circular RNA) has the characteristics of being abundant, evolutionarily conserved, and relatively stable in the cytoplasm, which enables it to have many functions and participate in gene expression regulation through various ways of action (21). We performed circRNA sequencing on rumen epithelial cell samples from the GAS and CON groups. By analyzing the differential expression of circRNA, we screened for significant differences in circRNA expression between the GAS group and the CON group. Among them, there were 10 significantly expressed differentially expressed circRNAs, of which 7 were significantly upregulated and 3 were significantly downregulated (Supplementary Table S5; Figure 7A).

Figure 7

Figure 7. Differential expression analysis of circRNA between GAS group and CON group. (A) Volcano map of circRNA expression differences between GAS group and CON group. The horizontal axis represents the fold change (log (B/A)) value of the transcript expression difference between different groups, while the vertical axis represents the P-value of the transcript expression change. The smaller the p-value, the greater the – log (p-value), and the more significant the difference. Red represents upregulated transcripts, green represents downregulated transcripts, and black represents non differential transcripts. (B) Histogram of host gene functional annotation classification for differentially expressed circRNA between GAS and CON groups. The horizontal axis represents the functional classification, while the vertical axis represents the number of genes within the classification (right) and their percentage in the total number of annotated genes (left). Light colors represent host genes, while dark colors represent all genes. (C) The top 30 functional scatter plots show significant enrichment of circRNA between the GAS group and the CON group. The vertical axis represents functional annotation information, while the horizontal axis represents the Rich factor corresponding to the function. The size of the Q-value is represented by the color of the dot. The smaller the Q-value, the closer the color is to red. The number of differentially expressed circRNA host genes is represented by the size of the dot.

After selecting differentially expressed circRNAs, GO analysis was conducted to investigate the distribution of differentially expressed circRNA target genes in annotation function between the GAS and CON groups, in order to elucidate the effect of feeding garlic skin containing diets on gene function. The target genes for differentially expressed circRNA are mainly involved in cellular processes, metabolic processes, biological regulation processes, and biological processes such as cell composition, tissue, or biogenesis. They were mainly located in the cellular and organelle parts, and their main molecular functions are focused on binding and catalytic activity (Figure 7B). To further test whether the target genes of differentially expressed circRNA are enriched in certain functions, we conducted functional enrichment analysis on the genes and selected the top 30 functions with the highest functional enrichment of differentially expressed circRNA target genes (Table 4; Figure 7C). The target genes for differentially expressed circRNA between the GAS and CON groups were mainly involved in the regulation of iron ions and immune responses, which is closely related to the efficacy and role of GAS itself. GAS contains a large amount of sulfur compounds, which can enhance the body’s immune system and effectively block the formation of lipid peroxidation (22). This is consistent with the above results.

Table 4

Table 4. 30 significantly enriched differential circRNAs in the GAS diet group.

3.6 LncRNA bioinformatics

LncRNA (Long Chain Non coding RNA) has a conserved secondary structure that can interact with proteins, DNA, and RNA, participate in the regulation of various biological processes, and play an important role in numerous life processes (23, 24). Differential analysis of transcript expression was performed on the rumen epithelial tissues of the GAS and CON groups. A total of 130 differentially expressed transcripts (lncRNA and mRNA) were screened (Supplementary Table S6; Figure 8A), with 46 significantly upregulated transcripts, including 7 significantly upregulated lncRNA, 39 mRNA, and 84 significantly downregulated transcripts, including 39 lncRNA and 45 mRNA.

Figure 8

Figure 8. Differential expression analysis of transcriptome between GAS group and CON group. (A) Volcano map of transcript expression differences between GAS group and CON group. (B) Histogram of functional annotation classification of genes corresponding to GAS and CON differential transcripts. (C) The top 30 functional scatter plots show significant enrichment of differentially expressed transcripts in the GAS and CON groups.

The annotation analysis of gene functions corresponding to differential transcripts revealed that the gene functions of differential transcripts are mainly involved in biological processes such as cellular processes, metabolic processes, and regulation of biological processes, located in positions such as cells, cell components, and organelles (Figure 8B). The main molecular functions are concentrated in binding and catalytic activities. Functional enrichment analysis examined the enrichment function of differentially expressed genes, and the corresponding gene functions of transcripts were mainly enriched in the immune regulatory response of the body (Table 5; Figure 8C). The allicin contained in GAS has bactericidal effects, which can enhance the immunity of livestock and poultry (25). This is consistent with the analysis results of gene function enrichment corresponding to transcripts.

Table 5

Table 5. Gene functions corresponding to 30 significantly enriched differential transcripts in the GAS group.

3.7 MiRNA bioinformatics

MiRNA (MicroRNA) is a 17-24 nt single stranded non coding RNA molecule that can interact with mRNA to affect the stability and translation of target mRNA, regulate gene expression, cell growth, development, and other biological processes (26, 27). The study of miRNA sequencing is of great significance for understanding the mechanisms of biological growth and development, as well as the occurrence and development of diseases. A total of 1,314 differentially expressed miRNAs were screened between the GAS and CON groups, of which 769 were significantly upregulated and 545 were significantly downregulated (Figure 9A).

Figure 9

Figure 9. Differential expression analysis of miRNA between GAS group and CON group. (A) Volcano map of miRNA expression differences between GAS group and CON group. (B) GAS group and CON group differentially expressed miRNA GO annotation classification histogram. (C) The functional scatter plot shows a significant difference in miRNA enrichment between the GAS group and the CON group, with a top 10 * 3 degree (biological process, cellular component, and molecular function each with a top 10 degree).

Functional annotation analysis of differentially expressed miRNA targeting mRNA genes revealed that genes differentially expressed in biological processes were mainly concentrated in cellular processes, single biological processes, biological regulation, and metabolic processes. The number of annotated genes in cell components with a high proportion was mainly concentrated in cells, organelles, and membranes, while protein binding and catalytic activity were higher in molecular functions annotated (Figure 9B). GO has three ontologies, with the highest enrichment level of top 10 in the molecular function, cellular component, and biological process of the gene (Table 6; Figure 9C). Compared with the CON group, the top 10 miRNA targeted mRNA genes in the GAS group were mainly enriched in transcriptional regulation, located in organelles and cell membranes, and the molecular functions were mainly protein, ATP, nucleotide, and DNA binding, consistent with the results of miRNA gene functional annotation.

Table 6

Table 6. Gene functions of 30 significantly enriched differential miRNAs targeting mRNA in the GAS group.

4 Discussion

4.1 The effect of GAS on the growth performance and intestinal tissue morphology

Sheep (Ovis aries) alongside goat, are considered as poor man’s cow and they play an important role in the household economy of a poor farmer (28). Sheep provides wool along with good quality meat and milk due to which its role in the backyard and commercial farming cannot be neglected. Many approaches are being adapted for the proper growth and health of sheep among which use of natural products as therapeutic and growth promoting substances is gaining popularity from the past few decades (29–31). Research has found that GAS has strong functions such as antioxidation, detoxification, sterilization, disease prevention, and aiding digestion. Adding an appropriate amount of GAS to feed can not only improve feed taste and increase feed utilization, but also enhance animal immunity and prevent disease occurrence (5).

In pig breeding, adding garlic powder to the basic diet can improve growth performance, meat quality, and regulate gut microbiota (32). In the diet of calves, supplementing with garlic extract can significantly increase feed intake, feed conversion rate, and average daily weight gain (33). Previous studies have found that adding GAS to the diet of fattening sheep can improve the average daily weight gain and feed conversion efficiency of fattening sheep (19). This is consistent with the early experimental results of garlic and its extract allicin in livestock and aquatic animals (including poultry) (33, 34). The bioactive compounds present in GAS are essentially polyphenols, which have a positive impact on energy metabolism (35). Therefore, the effect of GAS on the growth performance of fattening sheep can be partially attributed to the improvement of energy utilization efficiency. On this basis, we further investigated the mechanism of adding GAS to the diet to improve the production performance of fattening sheep.

The tissue structure of the gastrointestinal tract plays an important regulatory role in the physiological, metabolic, and immune functions of animal bodies (36, 37). The results showed that adding 8% GAS to the diet had no significant effect on the gastrointestinal structure of fattening sheep (Supplementary Tables S7, S8; Supplementary Figures S1, S2). In addition, studies have found that GAS can protect gastrointestinal health and inhibit nematode infections (38). Meanwhile, GAS can increase the number of cellulose degrading bacteria in the rumen (39). This indicates that GAS can promote the growth and health of fattening sheep by protecting gastrointestinal health, improving gastrointestinal digestion and emission capacity, rather than regulating the growth performance of fattening sheep by regulating changes in gastrointestinal tissue structure.

4.2 The effect of GAS on the fecal microbiota

The analysis of microbial diversity at the fecal genus level between the GAS and CON groups showed significant changes in the abundance of four microbial communities. Compared with the CON group, the GAS group significantly upregulated Methanobrevibacter. Methanobrevibacter in the intestine can reduce the accumulation of intestinal gases, maintain an anaerobic environment in the hindgut, utilize nutrients in the intestinal cavity, and compete with other microorganisms for symbiosis. This is of great significance for stabilizing the intestinal microbiota and protecting intestinal health (38). The downregulated bacteria included Akkermansia, Parasutterella, and Guggenheimella. Akkermansia, as a bacterium that helps resist obesity, can be reduced within a reasonable range of abundance in the body (40). Reducing Parasutterella in the intestine is beneficial for maintaining gastrointestinal ecological stability and increasing gut microbiota diversity, reducing intestinal and metabolic diseases, and promoting intestinal health (41). This indicates that adding GAS to the diet is beneficial for the proliferation of probiotics in the gut of fattening sheep, reducing the number of harmful bacteria, thereby improving the composition of gut microbiota, promoting nutrient absorption, and promoting the growth performance and health of fattening sheep.

4.3 The effect of GAS on serum antioxidant indicators

The levels of superoxide dismutase, peroxidase, and catalase in serum are important indicators for measuring the body’s antioxidant capacity (42, 43). MDA is the final product of lipid peroxidation reaction, which can cause cross-linking and polymerization of life molecules such as proteins and nucleic acids, and has cytotoxicity (44, 45). The higher the content of malondialdehyde, the greater the harm to the body (46). Compared with the CON group, the GAS group significantly increased serum GSH-Px in fattening sheep, decreased MDA (p < 0.05), and slightly higher SOD and T-AOC than the CON group. This indicates that adding GAS to the diet can improve the antioxidant capacity of fattening sheep, reduce energy consumption and disease occurrence, and improve the growth efficiency of fattening sheep.

4.4 The effect of GAS on the metabolomics of serum and urine

Through serum and urine metabolomics analysis, it was found that metabolites such as taurine, taurocholate, glycine, and uric acid were significantly upregulated in the GAS group. Taurine, as a regulatory amino acid, has multiple functions such as antioxidant, regulating energy metabolism, detoxification, and promoting digestion and absorption (47). Taurocholate is formed by the combination of various substances such as taurine, glycine, and bile acids, promoting the digestion and absorption of fatty acids and vitamins (48). Uric acid is a natural antioxidant that can combat oxidative stress, eliminate oxygen free radicals, and maintain the body’s immune function (49). In addition, carbohydrates such as xylitol, mannose, and D-fructose are significantly reduced. Among them, an increase in the content of xylitol and mannose can stimulate the gastrointestinal tract to a certain extent, leading to intestinal ringing, loss of appetite, and diarrhea (50). High levels of fructose can cause an increase in ROS levels, leading to oxidative stress in the body (51).

The metabolic pathways that show significant changes in serum mainly include arginine and proline metabolism, taurine metabolism and taurine metabolism, and pyrimidine metabolism. The main metabolic pathways in urine are tryptophan metabolism, pyrimidine metabolism, and riboflavin metabolism. Arginine and proline metabolism participate in multiple metabolic pathways, synthesize immunoglobulins, and improve the body’s immune function (52). Taurine metabolism and secondary taurine metabolism enhance the digestion and absorption of lipids in the gastrointestinal tract, and exhibit anti-inflammatory and antioxidant effects in diseases that affect animal production, such as animal heat stress, gastrointestinal injury, and mastitis (53). Pyrimidine metabolism is of great significance in maintaining nucleic acid balance and repairing damaged DNA, providing nitrogen or carbon sources for microbial growth (54). Tryptophan metabolism enhances intestinal immune function and prevents intestinal diseases (55). Riboflavin metabolism is involved in the metabolism of proteins, fats, and carbohydrates in the body, promoting animal growth, improving animal production performance, and cold tolerance. It is an essential nutrient for animals (56).

Based on metabolomics analysis of serum and urine, adding GAS to the diet of fattening sheep can affect the levels of metabolites in serum and urine, increase beneficial metabolite levels, reduce harmful metabolites, and lead to changes in related metabolic pathways. These metabolites and metabolic pathways are related to energy metabolism, protein synthesis, disease treatment, and the immune capacity of fattening sheep, improving their antioxidant, antibacterial, and disease resistance abilities, thereby enhancing their growth performance.

4.5 The effect of GAS on transcriptomics of rumen epithelial tissue

CirRNA, as a novel type of RNA, has a circular structure and is involved in biological processes such as protein synthesis, immunity, and metabolism, playing an important role in organisms (57). LncRNA is a non coding RNA with a length greater than 200 nt, involved in the regulation of various life activities (58). MiRNA is a type of non-coding single stranded RNA molecule with a length of approximately 22 nt encoded by endogenous genes, involved in biological processes such as metabolism, immune response, and inflammation treatment (59). Through transcriptome sequencing of rumen epithelial tissue, the function and structure of genes were studied at the transcriptional level, revealing the effect of feeding GAS on gene expression in fattening sheep. Compared with the CON group, the GAS group showed significant upregulation of 7 cirRNAs and significant downregulation of 3 cirRNAs; 7 significantly upregulated lncRNAs and 39 significantly downregulated lncRNAs; 769 miRNAs were significantly upregulated, and 545 miRNAs were significantly upregulated. GO enrichment analysis was performed on differentially expressed cirRNAs, lncRNAs, and miRNAs, and it was found that these differentially expressed cirRNAs, lncRNAs, and miRNAs mainly involve functions such as energy metabolism, protein synthesis, antioxidant regulation, and immune response. This is consistent with the antioxidant, antibacterial, anti-inflammatory, and digestive promoting effects of GAS, confirming that GAS can promote the digestive ability of fattening sheep, improve their immune ability, and have important significance for the growth performance and health of fattening sheep.

In recent years, studies have found that adding a certain amount of garlic to the daily feed of various animals significantly improves feed conversion rate, daily feed intake, and survival rate of young offspring, effectively reducing the incidence of diseases (7, 8). GAS is a by-product of garlic production, and burning it as waste can easily pollute the environment. However, GAS is also rich in allicin and sulfides, which can resist the growth of various bacteria, viruses, and fungi, and have strong antibacterial effects (5). This experiment added GAS to the diet of fattening sheep as feed, and found that adding GAS can improve the growth performance of fattening sheep. We explained how GAS improves the growth performance of fattening sheep from the perspectives of serum antioxidant capacity, fecal microbiota analysis, serum and urine metabolites, and transcriptomics. This provides a reference for the application of garlic by-product GAS as feed in animal production, and also provides a theoretical basis for GAS to replace antibiotics and growth promoting agents in animal production.

5 Conclusion

In summary, adding 8% garlic peel to the diet improved the growth performance of fattening lamb and changed the composition of gut microbiota. Compared with the CON group, the GAS group significantly increased the relative abundance of Metanobrevibrater in the gut microbiota, while the relative abundance of Akkermansia, Parasutterella, and Guggenheimella was significantly reduced. Analysis of serum antioxidant indicators showed that compared with the CON group, the GAS group significantly increased serum GSH-Px and decreased MDA in the fattening lamb serum (p < 0.05), SOD and T-AOC slightly increased. Serum and urine metabolomics analysis showed that differential metabolites mainly included amino acids, fatty acids, dipeptides, and carbohydrates; the main signaling pathways included amino acid metabolism (taurine and taurine metabolism pathway, D-glutamine and D-glutamate metabolism pathway, arginine and proline metabolism pathway, cysteine and methionine metabolism pathway, tryptophan metabolism pathway, and biosynthesis of valine, leucine, and isoleucine), pyrimidine metabolism, and riboflavin metabolism pathway. These metabolic pathways were closely related to the improvement of growth performance and health status of fattening lamb. The transcriptome sequencing of rumen epithelial cells showed that the biological processes involved in circRNA, transcripts, and miRNA target genes mainly focused on cellular processes, biological regulation, and metabolic processes. The cellular locations were mainly cells and organelles, and molecular functions were mainly enriched in catalytic activity and protein connections. Functional enrichment analysis showed that differential expression was enriched in iron ion regulation, immune regulation, and transcriptional regulation functions. These results fully indicate that adding 8% GAS diet to fattening lamb can regulate protein synthesis and energy metabolism, improve gut microbiota composition, enhance serum antioxidant capacity, enhance body immunity, and improve growth performance and health level of fattening lamb. This has good reference value for healthy breeding.

Data availability statement

The original contributions presented in the study are publicly available. These data can be found in the following repository: https://www.ncbi.nlm.nih.gov/bioproject, accession numbers: PRJNA1116814, PRJNA1050748, PRJNA1116343.

Ethics statement

The animal study was approved by Animal Care Committee of Anhui Agriculture University (SYXK(Wan)2016-007). The study was conducted in accordance with the local legislation and institutional requirements.

Author contributions

YX: Conceptualization, Data curation, Writing – original draft. MY: Data curation, Formal analysis, Investigation, Writing – review & editing. SS: Writing – review & editing. LW: Methodology, Project administration, Writing – review & editing. ZZ: Funding acquisition, Project administration, Writing – review & editing. YL: Software, Validation, Writing – review & editing. HC: Funding acquisition, Writing – review & editing.

Funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by Anhui Key Research and Development Program (2023j11020001), Anhui Natural Science Foundation (2208085MC75), and National Natural Science Foundation of China (32272875).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fvets.2024.1409518/full#supplementary-material

Footnotes

1. ^https://spssau.com/

References

1. Gul, ST, and Alsayeqh, AF. Probiotics as an alternative approach to antibiotics for safe poultry meat production. Pak Vet J. (2022) 42:285–91. doi: 10.29261/pakvetj/2022.061

Crossref Full Text | Google Scholar

2. Caliskan, GU, and Emin, N. Protective efficacy of fresh and aged macerated garlic oils in safflower oil against intra-abdominal adhesions in rats. Pak Vet J. (2023) 43:290–6. doi: 10.29261/pakvetj/2023.030

Crossref Full Text | Google Scholar

3. Del Rayo Camacho-Corona, M, Camacho-Morales, A, Góngora-Rivera, F, Escamilla-García, E, Morales-Landa, JL, Andrade-Medina, M, et al. Immunomodulatory effects of Allium sativum L. and its constituents against viral infections and metabolic diseases. Curr Top Med Chem. (2022) 22:109–31. doi: 10.2174/1568026621666211122163156

PubMed Abstract | Crossref Full Text | Google Scholar

4. Jiang, XY, Liang, JY, Jiang, SY, Zhao, P, Tao, F, Li, J, et al. Garlic polysaccharides: a review on their extraction, isolation, structural characteristics, and bioactivities. Carbohydr Res. (2022) 518:108599. doi: 10.1016/j.carres.2022.108599

PubMed Abstract | Crossref Full Text | Google Scholar

5. Singiri, JR, Swetha, B, Ben-Natan, A, and Grafi, G. What worth the garlic Peel. Int J Mol Sci. (2022) 23:2126. doi: 10.3390/ijms23042126

PubMed Abstract | Crossref Full Text | Google Scholar

6. Serrano-Jara, D, Rivera-Gomis, J, Tornel, JA, Jordán, MJ, Martínez-Conesa, C, and Pablo, MJC. Oregano essential oil and purple garlic powder effects on intestinal health, microbiota indicators and antimicrobial resistance as feed additives in weaning piglets. Animals (Basel). (2023) 14:111. doi: 10.3390/ani14010111

Crossref Full Text | Google Scholar

7. Aboubakr, M, Farag, A, Elfadadny, A, Alkafafy, M, Soliman, A, and Elbadawy, M. Allicin and lycopene possesses a protective effect against methotrexate-induced testicular toxicity in rats. Pak Vet J. (2023) 43:559–66. doi: 10.1007/s11356-023-28686-4

Crossref Full Text | Google Scholar

8. Xu, S, Liao, Y, Wang, Q, Liu, L, and Yang, W. Current studies and potential future research directions on biological effects and related mechanisms of allicin. Crit Rev Food Sci Nutr. (2023) 63:7722–48. doi: 10.1080/10408398.2022.2049691

PubMed Abstract | Crossref Full Text | Google Scholar

9. Huang, W, Yao, C, Liu, Y, Xu, N, Yin, Z, Xu, W, et al. Dietary Allicin improved the survival and growth of large yellow croaker (Larimichthys crocea) larvae via promoting intestinal development, alleviating inflammation and enhancing appetite. Front Physiol. (2020) 11:587674. doi: 10.3389/fphys.2020.587674

PubMed Abstract | Crossref Full Text | Google Scholar

10. Shi, X, Zhou, X, Chu, X, Wang, J, Xie, B, Ge, J, et al. Allicin improves metabolism in high-fat diet-induced obese mice by modulating the gut microbiota. Nutrients. (2019) 11:2909. doi: 10.3390/nu11122909

Crossref Full Text | Google Scholar

11. Nadeem, MS, Kazmi, I, Ullah, I, Muhammad, K, and Anwar, F. Allicin, an antioxidant and neuroprotective agent, ameliorates cognitive impairment. Antioxidants (Basel). (2021) 11:87. doi: 10.3390/antiox11010087

Crossref Full Text | Google Scholar

12. Bottje, WG. Oxidative metabolism and efficiency: the delicate balancing act of mitochondria. Poult Sci. (2019) 98:4223–30. doi: 10.3382/ps/pey405

PubMed Abstract | Crossref Full Text | Google Scholar

13. Jian, Z, Zeng, L, Xu, T, Sun, S, Yan, S, Yang, L, et al. Antibiotic resistance genes in bacteria: occurrence, spread, and control. J Basic Microbiol. (2021) 61:1049–70. doi: 10.1002/jobm.202100201

Crossref Full Text | Google Scholar

14. Seidavi, A, Tavakoli, M, Asroosh, F, Scanes, CG, Abd el-Hack, ME, Naiel, MAE, et al. Antioxidant and antimicrobial activities of phytonutrients as antibiotic substitutes in poultry feed. Environ Sci Pollut Res Int. (2022) 29:5006–31. doi: 10.1007/s11356-021-17401-w

PubMed Abstract | Crossref Full Text | Google Scholar

15. Yaqoob, MU, Wang, G, and Wang, M. An updated review on probiotics as an alternative of antibiotics in poultry – a review. Anim Biosci. (2022) 35:1109–20. doi: 10.5713/ab.21.0485

PubMed Abstract | Crossref Full Text | Google Scholar

16. Ding, D, Wang, B, Zhang, X, Zhang, J, Zhang, H, Liu, X, et al. The spread of antibiotic resistance to humans and potential protection strategies. Ecotoxicol Environ Saf. (2023) 254:114734. doi: 10.1016/j.ecoenv.2023.114734

PubMed Abstract | Crossref Full Text | Google Scholar

17. Al-Khalaifa, H, Al-Nasser, A, Al-Surayee, T, Al-Kandari, S, Al-Enzi, N, Al-Sharrah, T, et al. Effect of dietary probiotics and prebiotics on the performance of broiler chickens. Poult Sci. (2019) 98:4465–79. doi: 10.3382/ps/pez282

Crossref Full Text | Google Scholar

18. Tian, M, He, X, Feng, Y, Wang, W, Chen, H, Gong, M, et al. Pollution by antibiotics and antimicrobial resistance in LiveStock and poultry manure in China, and countermeasures. Antibiotics (Basel). (2021) 10:539. doi: 10.3390/antibiotics10050539

Crossref Full Text | Google Scholar

19. Zhu, W, Su, Z, Xu, W, Sun, HX, Gao, JF, Tu, DF, et al. Garlic skin induces shifts in the rumen microbiome and metabolome of fattening lambs. Animal. (2021) 15:100216. doi: 10.1016/j.animal.2021.100216

PubMed Abstract | Crossref Full Text | Google Scholar

20. Kuziel, GA, and Rakoff-Nahoum, S. The gut microbiome. Curr Biol. (2022) 32:R257–r264. doi: 10.1016/j.cub.2022.02.023

Crossref Full Text | Google Scholar

21. Zhou, WY, Cai, ZR, Liu, J, Wang, DS, Ju, HQ, and Xu, RH. Circular RNA: metabolism, functions and interactions with proteins. Mol Cancer. (2020) 19:172. doi: 10.1186/s12943-020-01286-3

PubMed Abstract | Crossref Full Text | Google Scholar

22. Panyod, S, Wu, WK, Ho, CT, Lu, KH, Liu, CT, Chu, YL, et al. Diet supplementation with Allicin protects against alcoholic fatty liver disease in mice by improving anti-inflammation and Antioxidative functions. J Agric Food Chem. (2016) 64:7104–13. doi: 10.1021/acs.jafc.6b02763

PubMed Abstract | Crossref Full Text | Google Scholar

23. Bridges, MC, Daulagala, AC, and Kourtidis, A. LNCcation: lncRNA localization and function. J Cell Biol. (2021) 220:9045. doi: 10.1083/jcb.202009045

PubMed Abstract | Crossref Full Text | Google Scholar

24. Schmitz, SU, Grote, P, and Herrmann, BG. Mechanisms of long noncoding RNA function in development and disease. Cell Mol Life Sci. (2016) 73:2491–509. doi: 10.1007/s00018-016-2174-5

PubMed Abstract | Crossref Full Text | Google Scholar

25. Borlinghaus, J, Albrecht, F, Gruhlke, M, Nwachukwu, I, and Slusarenko, A. Allicin: chemistry and biological properties. Molecules. (2014) 19:12591–618. doi: 10.3390/molecules190812591

PubMed Abstract | Crossref Full Text | Google Scholar

26. Correia de Sousa, M, Gjorgjieva, M, Dolicka, D, Sobolewski, C, and Foti, M. Deciphering miRNAs' action through miRNA editing. Int J Mol Sci. (2019) 20:249. doi: 10.3390/ijms20246249

PubMed Abstract | Crossref Full Text | Google Scholar

27. Saliminejad, K, Khorram Khorshid, HR, Soleymani Fard, S, and Ghaffari, SH. An overview of microRNAs: biology, functions, therapeutics, and analysis methods. J Cell Physiol. (2019) 234:5451–65. doi: 10.1002/jcp.27486

PubMed Abstract | Crossref Full Text | Google Scholar

28. Hussein, SN, Ibrahim, AA, and Shukur, MS. Molecular identification of Sarcocystis species in sheep (Ovis aries) and goats (Capra hircus) of Duhok Province, Iraq. Pakistan Vet J. (2023) 43:248–54. doi: 10.3390/ani12162048

Crossref Full Text | Google Scholar

29. Okada, S, Inabu, Y, Miyamoto, H, Suzuki, K, Kato, T, Kurotani, A, et al. Estimation of silent phenotypes of calf antibiotic dysbiosis. Sci Rep. (2023) 13:6359. doi: 10.1038/s41598-023-33444-0

PubMed Abstract | Crossref Full Text | Google Scholar

30. Khan, A, Saleemi, MK, Ali, F, Abubakar, M, Hussain, R, Abbas, RZ, et al. Pathophysiology of peste des petits ruminants in sheep (Dorper & Kajli) and goats (Boer & Beetal). Microb Pathog. (2018) 117:139–47. doi: 10.1016/j.micpath.2018.02.009

PubMed Abstract | Crossref Full Text | Google Scholar

31. Attia, YA, Hassan, RA, Addeo, NF, Bovera, F, Alhotan, RA, Al-qurashi, AD, et al. Effects of Spirulina platensis and/or Allium sativum on antioxidant status, immune response, gut morphology, and intestinal lactobacilli and coliforms of heat-stressed broiler chicken. Vet Sci. (2023) 10:678. doi: 10.3390/vetsci10120678

Crossref Full Text | Google Scholar

32. Janz, JA, Morel, PCH, Wilkinson, BHP, and Purchas, RW. Preliminary investigation of the effects of low-level dietary inclusion of fragrant essential oils and oleoresins on pig performance and pork quality. Meat Sci. (2007) 75:350–5. doi: 10.1016/j.meatsci.2006.06.027

PubMed Abstract | Crossref Full Text | Google Scholar

33. Huang, RH, Qiu, XS, Shi, FX, Hughes, CL, Lu, ZF, and Zhu, WY. Effects of dietary allicin on health and growth performance of weanling piglets and reduction in attractiveness of faeces to flies. Animal. (2011) 5:304–11. doi: 10.1017/S1751731110001953

PubMed Abstract | Crossref Full Text | Google Scholar

34. Gong, HZ, Wu, M, Lang, WY, Yang, M, Wang, JH, Wang, YQ, et al. Effects of laying breeder hens dietary β-carotene, curcumin, allicin, and sodium butyrate supplementation on the growth performance, immunity, and jejunum morphology of their offspring chicks. Poult Sci. (2020) 99:151–62. doi: 10.3382/ps/pez584

PubMed Abstract | Crossref Full Text | Google Scholar

35. Lan, W, and Yang, C. Ruminal methane production: associated microorganisms and the potential of applying hydrogen-utilizing bacteria for mitigation. Sci Total Environ. (2019) 654:1270–83. doi: 10.1016/j.scitotenv.2018.11.180

Crossref Full Text | Google Scholar

36. Wang, S, Wang, L, Fan, X, Yu, C, Feng, L, and Yi, L. An insight into diversity and functionalities of gut microbiota in insects. Curr Microbiol. (2020) 77:1976–86. doi: 10.1007/s00284-020-02084-2

Crossref Full Text | Google Scholar

37. Taylor, AM, Thompson, SV, Edwards, CG, Musaad, SMA, Khan, NA, and Holscher, HD. Associations among diet, the gastrointestinal microbiota, and negative emotional states in adults. Nutr Neurosci. (2020) 23:983–92. doi: 10.1080/1028415X.2019.1582578

PubMed Abstract | Crossref Full Text | Google Scholar

38. Ding, H, Ao, C, and Zhang, X. Potential use of garlic products in ruminant feeding: a review. Anim Nutr. (2023) 14:343–55. doi: 10.1016/j.aninu.2023.04.011

PubMed Abstract | Crossref Full Text | Google Scholar

39. Zhong, R, Xiang, H, Cheng, L, Zhao, C, Wang, F, Zhao, X, et al. Effects of feeding garlic powder on growth performance, rumen fermentation, and the health status of lambs infected by gastrointestinal nematodes. Animals (Basel). (2019) 9:102. doi: 10.3390/ani9030102

Crossref Full Text | Google Scholar

40. Cani, PD, Depommier, C, Derrien, M, Everard, A, and de Vos, WM. Akkermansia muciniphila: paradigm for next-generation beneficial microorganisms. Nat Rev Gastroenterol Hepatol. (2022) 19:625–37. doi: 10.1038/s41575-022-00631-9

PubMed Abstract | Crossref Full Text | Google Scholar

41. Wang, Y, Tao, H, Huang, H, Xiao, Y, Wu, X, Li, M, et al. The dietary supplement Rhodiola crenulata extract alleviates dextran sulfate sodium-induced colitis in mice through anti-inflammation, mediating gut barrier integrity and reshaping the gut microbiome. Food Funct. (2021) 12:3142–58. doi: 10.1039/D0FO03061A

PubMed Abstract | Crossref Full Text | Google Scholar

42. Purba, RAP, Suong, NTM, Paengkoum, S, Schonewille, JT, and Paengkoum, P. Dietary inclusion of anthocyanin-rich black cane silage treated with ferrous sulfate heptahydrate reduces oxidative stress and promotes tender meat production in goats. Front Vet Sci. (2022) 9:969321. doi: 10.3389/fvets.2022.969321

PubMed Abstract | Crossref Full Text | Google Scholar

43. Ban, C, Paengkoum, S, Yang, S, Tian, X, Thongpea, S, Purba, RAP, et al. Feeding meat goats mangosteen (Garcinia mangostanaL.) peel rich in condensed tannins, flavonoids, and cinnamic acid improves growth performance and plasma antioxidant activity under tropical conditions. J Appl Anim Res. (2022) 50:307–15. doi: 10.1080/09712119.2022.2068557

Crossref Full Text | Google Scholar

44. Purba, RAP, and Paengkoum, P. Exploring the phytochemical profiles and antioxidant, antidiabetic, and Antihemolytic properties of Sauropus androgynus dried leaf extracts for ruminant health and production. Molecules. (2022) 27:27. doi: 10.3390/molecules27238580

Crossref Full Text | Google Scholar

45. Purba, RAP, and Paengkoum, P. Farang (Psidium guajava L.) dried leaf extracts: phytochemical profiles, antioxidant, anti-diabetic, and anti-hemolytic properties for ruminant health and production. Molecules. (2022) 27:27. doi: 10.3390/molecules27248987

Crossref Full Text | Google Scholar

46. Del Rio, D, Stewart, AJ, and Pellegrini, N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. (2005) 15:316–28. doi: 10.1016/j.numecd.2005.05.003

PubMed Abstract | Crossref Full Text | Google Scholar

47. Ma, J, Yang, Z, Jia, S, and Yang, R. A systematic review of preclinical studies on the taurine role during diabetic nephropathy: focused on anti-oxidative, anti-inflammation, and anti-apoptotic effects. Toxicol Mech Methods. (2022) 32:420–30. doi: 10.1080/15376516.2021.2021579

PubMed Abstract | Crossref Full Text | Google Scholar

48. Yu, X, Meng, X, Yan, Y, Wang, H, and Zhang, L. Extraction of Naringin from pomelo and its therapeutic potentials against hyperlipidemia. Molecules. (2022) 27:27. doi: 10.3390/molecules27249033

Crossref Full Text | Google Scholar

49. Kumar, B, Kumawat, BL, Khan, FA, das, GK, Maurya, SK, Chandra, P, et al. Comparative analysis of biochemical, hormonal, and mineral compositions of preovulatory and cystic ovarian follicles in buffalo during the non-breeding season. Zygote. (2023) 31:246–52. doi: 10.1017/S0967199423000084

PubMed Abstract | Crossref Full Text | Google Scholar

50. Zhang, Y, Vera, JM, Xie, D, Serate, J, Pohlmann, E, Russell, JD, et al. Multiomic fermentation using chemically defined synthetic Hydrolyzates revealed multiple effects of lignocellulose-derived inhibitors on cell physiology and xylose utilization in Zymomonas mobilis. Front Microbiol. (2019) 10:2596. doi: 10.3389/fmicb.2019.02596

PubMed Abstract | Crossref Full Text | Google Scholar

51. Loh, CH, Kuo, W-W, Lin, S-Z, Shih, C-Y, Lin, P-Y, Situmorang, JH, et al. Corrigendum to “PKC-δ-dependent mitochondrial ROS attenuation is involved as 9-OAHSA combats lipoapotosis in rat hepatocytes induced by palmitic acid and in Syrian hamsters induced by high-fat high-cholesterol high-fructose diet” [Toxicology and Applied Pharmacology, 470, (2023), 116557]. Toxicol Appl Pharmacol. (2023) 476:116658. doi: 10.1016/j.taap.2023.116658

Crossref Full Text | Google Scholar

52. Wu, G, Bazer, FW, Burghardt, RC, Johnson, GA, Kim, SW, Knabe, DA, et al. Proline and hydroxyproline metabolism: implications for animal and human nutrition. Amino Acids. (2011) 40:1053–63. doi: 10.1007/s00726-010-0715-z

PubMed Abstract | Crossref Full Text | Google Scholar

53. Wang, Z, Ohata, Y, Watanabe, Y, Yuan, Y, Yoshii, Y, Kondo, Y, et al. Taurine improves lipid metabolism and increases resistance to oxidative stress. J Nutr Sci Vitaminol (Tokyo). (2020) 66:347–56. doi: 10.3177/jnsv.66.347

PubMed Abstract | Crossref Full Text | Google Scholar

54. Garavito, MF, Narváez-Ortiz, HY, and Zimmermann, BH. Pyrimidine metabolism: dynamic and versatile pathways in pathogens and cellular development. J Genet Genomics. (2015) 42:195–205. doi: 10.1016/j.jgg.2015.04.004

PubMed Abstract | Crossref Full Text | Google Scholar

55. Xue, C, Li, G, Zheng, Q, Gu, X, Shi, Q, Su, Y, et al. Tryptophan metabolism in health and disease. Cell Metab. (2023) 35:1304–26. doi: 10.1016/j.cmet.2023.06.004

PubMed Abstract | Crossref Full Text | Google Scholar

56. Suwannasom, N, Kao, I, Pruß, A, Georgieva, R, and Bäumler, H. Riboflavin: the health benefits of a forgotten natural vitamin. Int J Mol Sci. (2020) 21:950. doi: 10.3390/ijms21030950

PubMed Abstract | Crossref Full Text | Google Scholar

57. Liu, CX, and Chen, LL. Circular RNAs: characterization, cellular roles, and applications. Cell. (2022) 185:2390. doi: 10.1016/j.cell.2022.06.001

PubMed Abstract | Crossref Full Text | Google Scholar

58. Gudenas, BL, and Wang, L. Prediction of LncRNA subcellular localization with deep learning from sequence features. Sci Rep. (2018) 8:16385. doi: 10.1038/s41598-018-34708-w

PubMed Abstract | Crossref Full Text | Google Scholar

59. Mehta, A, and Baltimore, D. MicroRNAs as regulatory elements in immune system logic. Nat Rev Immunol. (2016) 16:279–94. doi: 10.1038/nri.2016.40

PubMed Abstract | Crossref Full Text | Google Scholar