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        <title>Frontiers in Acoustics | Acoustofluidics section | New and Recent Articles</title>
        <link>https://www.frontiersin.org/journals/acoustics/sections/acoustofluidics</link>
        <description>RSS Feed for Acoustofluidics section in the Frontiers in Acoustics journal | New and Recent Articles</description>
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        <pubDate>2026-05-14T05:44:40.88+00:00</pubDate>
        <ttl>60</ttl>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/facou.2025.1568083</guid>
        <link>https://www.frontiersin.org/articles/10.3389/facou.2025.1568083</link>
        <title><![CDATA[Measurement of dynamic electrokinetic effects at the glass/electrolyte interface using a mega-Hertz-level mechanical wave]]></title>
        <pubdate>2025-06-05T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Sudeepthi Aremanda</author><author>Yifan Li</author><author>Gideon Onuh</author><author>Ofer Manor</author>
        <description><![CDATA[We measured the dynamic electrical properties of a spontaneously charged glass surface in an electrolyte solution by using a MHz-level surface acoustic wave (SAW) actuator to introduce a same-frequency mechanical wave into the glass substrate. The mechanical wave vibrated ions in the nanometer-thick electrical double layer (EDL) to appear at the glass/electrolyte interface. The out-of-equilibrium EDL leaked an electrical field, which was modulated by ion vibration frequency to reveal the presence of ions and their dynamic motion. A previous study excited EDLs on the piezoelectric lithium niobate substrate of a SAW actuator in contact with an electrolyte solution, but it remained unclear whether the mechanical or electrical components of the SAW in the piezoelectric substrate dominated the EDL excitation. Here, we isolated the SAW mechanical component in glass and showed that it introduces a similar ion electrokinetic vibration in the excited EDL at the glass/electrolyte interface using sodium nitrate and potassium chloride solutions as electrolytes. The measured electrical field leakage spectra were of similar magnitude to those measured in the previous study and exhibited similar non-monotonic behaviors, taking local maxima where the SAW period (the inverse of its frequency) was synchronized with the ion relaxation times in the EDL. At these frequencies, the synchronization maximized ion vibration displacement, thereby amplifying the electrical field leakage. Our findings may be used to study the electrokinetic properties of solid surfaces and ion dynamics in EDLs. Moreover, SAW-actuated fluidic platforms may support out-of-equilibrium EDLs relevant to ion-selective membranes and the film stability of electrolyte solutions.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/facou.2025.1544464</guid>
        <link>https://www.frontiersin.org/articles/10.3389/facou.2025.1544464</link>
        <title><![CDATA[Sorting droplets beyond the Rayleigh limit under acoustic-gravity forces: a theoretical approach]]></title>
        <pubdate>2025-03-20T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Jeyapradhap Thirisangu</author><author>Karthick Subramani</author>
        <description><![CDATA[The physics of droplets beyond the Rayleigh limit offers a novel acoustic sorting method that is fundamentally different from the techniques applied to sort droplets within the Rayleigh limit. Using this “acoustic sorting beyond Rayleigh limit” method, we theoretically demonstrate that by controlling the frequency (f) and acoustic energy density (Eac), smaller droplets can be suspended while larger ones settle against gravity, or larger droplets can be suspended while smaller ones settle. Intriguingly, this method also enables the suspension of intermediate-sized droplets, allowing both smaller and larger droplets to settle simultaneously. Furthermore, acoustic sorting is demonstrated for droplets of identical size but with varying interfacial tensions.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/facou.2024.1512579</guid>
        <link>https://www.frontiersin.org/articles/10.3389/facou.2024.1512579</link>
        <title><![CDATA[Direct observation of small scale capillary wave turbulence using high speed digital holographic microscopy]]></title>
        <pubdate>2024-12-20T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>William Connacher</author><author>Jeremy Orosco</author><author>Oliver T. Schmidt</author><author>James Friend</author>
        <description><![CDATA[IntroductionIt is now known that capillary waves driven upon a fluid interface by high frequency (>1 MHz) ultrasound exhibit capillary wave turbulence: the appearance of waves with phase and wavelength far removed from the excitation signal that drives them. These waves are responsible in significant part for atomization, a useful application for ultrasound, though the physics responsible for their appearance is poorly understood.MethodsWe use high-speed digital holographic microscopy to observe these capillary waves, an important step towards understanding their generation and atomization phenomena.ResultsWe observe Zakharov-Kolmogorov weak wave turbulence for a limited range of input power, and find broader turbulence phenomena outside this range. We see discrete thresholds as the input power is increased, where higher and higher frequency responses are driven in the capillary waves with sudden onset between regimes.DiscussionWe employed spatial analysis to find extensions of the capillary wave response to higher frequencies, suggesting there is additional information in the spatial distribution of the capillary wave that is rarely if ever measured. We verified via frequency modulation that nonlinear resonance broadening is present, which undermines the use of Faraday wave or parametric wave theories to characterize these waves, important in the context of atomization which is now, definitively, not a Faraday wave process.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/facou.2024.1502136</guid>
        <link>https://www.frontiersin.org/articles/10.3389/facou.2024.1502136</link>
        <title><![CDATA[Detaching cells in culture medium using forced vibration for removing a centrifugation from culture process]]></title>
        <pubdate>2024-11-29T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Hayato Shimoguchi</author><author>Chikahiro Imashiro</author><author>Kenjiro Takemura</author>
        <description><![CDATA[Recent advancements in cell culture have significantly impacted various fields, including drug discovery and regenerative medicine. Consequently, there is an increasing need to minimize the contamination risks and labor involved in cell culture processes. Traditional cell detachment methods typically employ proteolytic enzymes followed by centrifugation to remove these enzymes after cell detachment. This process often requires numerous manual interventions which can lead to potential contamination and deterioration of cell quality. In this study, we propose a novel cell detachment method that eliminates the need for centrifugation even with less trypsinization time. Our approach involves reducing the duration of trypsinization, collecting the trypsin before complete cell detachment, and subsequently detaching the cells using forced vibration within the culture medium. We conducted experiments to optimize the enzyme treatment time and vibration conditions. Our results demonstrated that this method achieved an 82.8% detachment rate of cells from the culture surface. These findings indicate that the proposed cell detachment technique is effective in removing cells from the culture substrate and the following subculture process without the need for centrifugation.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/facou.2023.1326759</guid>
        <link>https://www.frontiersin.org/articles/10.3389/facou.2023.1326759</link>
        <title><![CDATA[Integrating ultrasonic neuromodulation with fiber photometry]]></title>
        <pubdate>2023-12-20T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Aditya Vasan</author><author>Uri Magaram</author><author>Janki Patel</author><author>James Friend</author><author>Sreekanth H. Chalasani</author>
        <description><![CDATA[Ultrasound has been used to modulate neural activity in rodents and primates; however, combining ultrasound stimulation with in vivo imaging in freely moving animals has been challenging. Here, we design and validate a transducer to overcome these challenges in the rodent. We develop a head-mounted ultrasound transducer that can be combined with a fiber photometry system. This combination allows us to monitor ultrasound-evoked responses in striatal neurons in awake and freely moving animals. Together, this system allows for a high-resolution analysis of ultrasound-evoked biology at the level of both neural circuits and behavior in freely moving animals, critical to providing a mechanistic understanding of ultrasound neuromodulation.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/facou.2023.1261027</guid>
        <link>https://www.frontiersin.org/articles/10.3389/facou.2023.1261027</link>
        <title><![CDATA[Acoustofluidics]]></title>
        <pubdate>2023-10-02T00:00:00Z</pubdate>
        <category>Mini Review</category>
        <author>James Friend</author>
        <description><![CDATA[The propagation of acoustic waves in fluids and solids produces fascinating phenomena that have been studied since the late 1700s and through to today, where it is finding broad application in manipulating fluids and particles at the micro to nano-scale. Due to the recent and rapid increase in application frequencies and reduction in the scale of devices to serve this new need, discrepancies between theory and reality have driven new discoveries in physics that are underpinning the burgeoning discipline. While many researchers are continuing to explore the use of acoustic waves in microfluidics, some are exploring vastly smaller scales, to nanofluidics and beyond. Because many of the applications incorporate biological material—organelles, cells, tissue, and organs—substantial effort is also being invested in understanding how ultrasound interacts with these materials. Surprisingly, there is ample evidence that ultrasound can be used to directly drive cellular responses, producing a new research direction beyond the established efforts in patterning and agglomerating cells to produce tissue. We consider all these aspects in this mini-review after a brief introduction to acoustofluidics as an emerging research discipline.]]></description>
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