AUTHOR=Wang Yixuan , Liu Yonghong , Jin Zhaohui , Liu Cui , Yu Xin , Chen Keke , Meng Detao , Liu Aixian , Fang Boyan TITLE=Association Between Mitochondrial Function and Rehabilitation of Parkinson's Disease: Revealed by Exosomal mRNA and lncRNA Expression Profiles JOURNAL=Frontiers in Aging Neuroscience VOLUME=Volume 14 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2022.909622 DOI=10.3389/fnagi.2022.909622 ISSN=1663-4365 ABSTRACT=Rehabilitation has been proposed as a valid measure complementary to management of Parkinson’s disease (PD). However, the mechanism underlying which is not clear yet. The differential expressions of exosomal messenger RNA (mRNA) and long noncoding RNAs (lncRNAs) may play a critical role in PD progression and rehabilitation. For the purpose to compare the differential expressions of exosomal mRNAs and lncRNAs, patients with PD (PWPs, Hoehn & Yahr stages 1.5-2.5, n = 6) and age‐ and sex‐matched healthy controls (HCs, n = 6) were included in this study. All PWPs received 2-week rehabilitation treatment in hospital, which seemingly led to improvement in both the motor and non-motor functions. A set of differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs) extracted from exosomes in blood samples via next‐generation sequencing (NGS) were screened out. Compared to HCs, 2337 versus 701 mRNAs and 1278 versus 445 lncRNAs were significantly upregulated and significantly downregulated, respectively in pre-rehabilitation (pre-rehab) PWPs; 2490 versus 629 mRNAs and 1561 versus 370 lncRNAs were significantly upregulated and significantly downregulated, respectively, in post-rehabilitation (post-rehab) PWPs. Compared to pre-rehab PWPs, 606 versus 1056 mRNAs and 593 versus 1136 lncRNAs were significantly upregulated and significantly downregulated, respectively, in post-rehab PWPs. Overall, 14 differentially expressed mRNAs (DEmRNAs) and 73 differentially expressed lncRNAs (DElncRNAs) were expressed in the blood exosomes of HCs, pre- and post-rehab PWPs, simultaneously. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses identified 243 significantly co-expressed lncRNA-mRNA pairs. One DEmRNA of interest (ENSG00000099795, NDUFB7) and three corresponding DElncRNAs (ENST00000564683, ENST00000570408, ENST00000628340) were positively related. Quantitative real-time polymerase chain reaction (qRT-PCR) validated that the expression levels of NDUFB7 mRNA and the 3 DElncRNAs increased significantly in pre-rehab PWPs, but decreased significantly in post-rehab PWPs compared to HCs. NDUFB7 mRNA is a marker related to mitochondrial respiration. It is reasonably believed that mitochondrial function is associated with PD rehabilitation, and the mitochondrial pathway may involve in the pathogenesis of PD.