AUTHOR=Li Xinyu , Zeng Huimei , Durairaj Pradeepraj , Wen Weihuan , Li Tianpeng , Zhao Yanru , Liu Yang , Liu Xue , Zhan Lingpeng , Rao Lang , Yuan Wen , Guo Tengfei , Shen Weijun , Cai Hui , Chen Zhicheng 

TITLE=Fully synthetic phosphorylated Tau181, Tau217, and Tau231 calibrators for Alzheimer’s disease diagnosis

JOURNAL=Frontiers in Aging Neuroscience

VOLUME=Volume 15 - 2023

YEAR=2024

URL=https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2023.1340706

DOI=10.3389/fnagi.2023.1340706

ISSN=1663-4365

ABSTRACT=Background: The calibrator in immunoassay plays an essential role in diagnosing Alzheimer's disease (AD). Presently, the most well-studied biomarkers for AD diagnosis are three phosphorylated Tau (p-Tau): p-Tau231, p-Tau217, and p-Tau181. Glycogen synthase-3beta (GSK3β)-phosphorated Tau-441 is the most commonly used calibrator for p-Tau immunoassays. However, the batch-to-batch inconsistency of the commonly used GSK3β-phosphorylated Tau-441 limits its clinical application.Methods: We have successfully generated and characterized 61 Tau monoclonal antibodies (mAbs) with distinct epitopes by using the hybridoma technique and employed them as capture or detection antibodies for p-Tau immunoassays. Through chemical synthesis, we synthesized calibrators, which are three peptides comprising two epitopes, as well as capture and detection antibody epitopes, for application in immunoassays that detect p-Tau231, p-Tau217, and p-Tau181. The novel calibrators were applied to Enzyme-linked immunosorbent assay (ELISA) and Single-molecule array (Simoa) platforms to validate their applicability and establish a range of p-Tau immunoassays.