AUTHOR=Mares Jason , Costa Ana Paula , Dartora William J. , Wartchow Krista M. , Lazarian Artur , Bennett David A. , Nuriel Tal , Menon Vilas , McIntire Laura Beth J. TITLE=Brain and serum lipidomic profiles implicate Lands cycle acyl chain remodeling association with APOEε4 and mild cognitive impairment JOURNAL=Frontiers in Aging Neuroscience VOLUME=Volume 16 - 2024 YEAR=2024 URL=https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2024.1419253 DOI=10.3389/fnagi.2024.1419253 ISSN=1663-4365 ABSTRACT=At least one-third of the identified risk alleles from Genome-Wide Association Studies (GWAS) of Alzheimer's disease (AD) are involved in lipid metabolism, lipid transport, or direct lipid binding. In fact, a common genetic variant (e4) in a cholesterol and phospholipid transporter, Apolipoprotein E (APOEε4), is the primary genetic risk factor for late-onset AD. In addition to genetic variants, lipidomic studies have reported severe metabolic dysregulation in human autopsy brain tissue, cerebrospinal fluid, blood, and multiple mouse models of AD. We aimed to identify an overarching metabolic pathway in lipid metabolism by integrating analyses of lipidomics and transcriptomics from the Religious Order Study and Rush Memory Aging Project (ROSMAP). Coordinated differences in lipids were found to be dysregulated in association with both mild cognitive impairment (MCI) and carriership of the APOEe4 genotype. Interestingly, these correlations were weakened when adjusting for education. Indeed, in this cohort, the cognitively non-impaired APOEε4 carriers have higher education levels in the ROSMAP cohort, suggesting that this lipid signature may be associated with a resilience phenotype. Network correlation analysis identified multiple differential lipids within a single module that are substrates and products in the Lands Cycle for acyl chain remodeling. In addition, our analyses identified multiple genes in the Lands Cycle acyl chain remodeling pathway, which were associated with cognitive decline independent of amyloid-β (Ab) load and tau tangle pathologies. Our studies highlight the critical differences in acyl chain remodeling in brain tissue from APOEe4 carriers and individual non-carriers with MCI. A coordinated lipid profile shift in dorsolateral prefrontal cortex from both APOEe4 carriers and MCI suggests differences in lipid metabolism occur early in disease stage and highlights lipid homeostasis as a tractable target for early disease modifying intervention.