https://www.frontiersin.org/journals/analytical-science/sections/omics RSS Feed for Omics section in the Frontiers in Analytical Science journal | New and Recent Articles en-us Frontiers Feed Generator,version:1 2026-05-10T13:34:15.03+00:00 60 https://www.frontiersin.org/articles/10.3389/frans.2025.1596778 https://www.frontiersin.org/articles/10.3389/frans.2025.1596778 2025-04-04T00:00:00Z Editorial Binesh UnnikrishnanChih-Ching HuangHuan-Tsung ChangElefteria PsillakisFederico MariniJohn T. Grant https://www.frontiersin.org/articles/10.3389/frans.2024.1512573 https://www.frontiersin.org/articles/10.3389/frans.2024.1512573 2025-01-03T00:00:00Z Perspective Youngki YouGina ManyErnesto S. Nakayasu https://www.frontiersin.org/articles/10.3389/frans.2024.1397810 https://www.frontiersin.org/articles/10.3389/frans.2024.1397810 2024-09-10T00:00:00Z Original Research Karel BezstarostiLennart Van der WalWouter A. S. DoffJeroen A. A. Demmers https://www.frontiersin.org/articles/10.3389/frans.2024.1425190 https://www.frontiersin.org/articles/10.3389/frans.2024.1425190 2024-08-01T00:00:00Z Original Research Hugo AmedeiNiels Benjamin PaulBrian FooLisa NeuenrothStephan E. LehnartHenning UrlaubChristof Lenz 80 fractions, however, translates into significant demands on sample quantity and mass spectrometric instrument time, and represents a significant barrier to experimental replication and the use of scarce sample material (ex. patient biopsies).MethodsWe developed mini-Complexome Profiling (mCP), a streamlined workflow with reduced requirements for fractionation and, thus, biological material and laboratory and instrument time. Soluble and membrane-associated protein complexes are extracted from biological material under mild conditions, and fractionated by Blue Native electrophoresis using commercial equipment. Each fraction is analysed by data-independent acquisition mass spectrometry, and known protein complexes are detected based on the coelution of known components using a novel R package with a controlled false discovery rate approach. The tool is available to the community on a GitHub repository.ResultsmCP was benchmarked using HEK293 cell lysate and exhibited performance similar to established workflows, but from a significantly reduced number of fractions. We then challenged mCP by performing comparative complexome analysis of cardiomyocytes isolated from different chambers from a single mouse heart, where we identified subtle chamber-specific changes in mitochondrial OxPhos complexes.DiscussionThe reduced sample and instrument time requirements open up new applications of co-fractionation mass spectrometry, specifically for the analysis of sparse samples such as human patient biopsies. The ability to identify subtle changes between similar tissue types (left/right ventricular and atrial cardiomyocytes) serves as a proof of principle for comparative analysis of mild/asymptomatic disease states.]]> https://www.frontiersin.org/articles/10.3389/frans.2023.1278170 https://www.frontiersin.org/articles/10.3389/frans.2023.1278170 2023-11-07T00:00:00Z Editorial Kim K. HixsonAmit DhingraFrancisco Dini-AndreoteMitchel J. DoktyczTimothy J. TschaplinskiLjiljana Paša-Tolić https://www.frontiersin.org/articles/10.3389/frans.2023.1306435 https://www.frontiersin.org/articles/10.3389/frans.2023.1306435 2023-10-13T00:00:00Z Editorial Sophie Ayciriex https://www.frontiersin.org/articles/10.3389/frans.2023.1258558 https://www.frontiersin.org/articles/10.3389/frans.2023.1258558 2023-09-25T00:00:00Z Review Manivannan MadhuS. SanthoshkumarWei-Bin TsengWei-Lung Tseng https://www.frontiersin.org/articles/10.3389/frans.2023.1205583 https://www.frontiersin.org/articles/10.3389/frans.2023.1205583 2023-08-29T00:00:00Z Original Research Seanna HewittRishikesh GhogareWilliam TroxelElvir TenicDaylen IsaacAmit Dhingra https://www.frontiersin.org/articles/10.3389/frans.2023.1259466 https://www.frontiersin.org/articles/10.3389/frans.2023.1259466 2023-08-17T00:00:00Z Editorial Mowei ZhouNicholas B. BorottoHuilin LiTara L. PukalaIan K. Webb https://www.frontiersin.org/articles/10.3389/frans.2023.1124741 https://www.frontiersin.org/articles/10.3389/frans.2023.1124741 2023-07-13T00:00:00Z Original Research Edison Zhamungui SánchezHassan HijaziJana HaidarEnrica MecarelliElda BaudaIsabelle Petit-HärtleinJean-Marie TeulonJean-Luc PellequerElisabetta Boeri Erba https://www.frontiersin.org/articles/10.3389/frans.2023.1186623 https://www.frontiersin.org/articles/10.3389/frans.2023.1186623 2023-06-22T00:00:00Z Perspective Ji KangMeena SeshadriKellye A. Cupp-SuttonSi Wu https://www.frontiersin.org/articles/10.3389/frans.2023.1073540 https://www.frontiersin.org/articles/10.3389/frans.2023.1073540 2023-06-07T00:00:00Z Original Research Federico SabaJulien MariethozFrederique Lisacek https://www.frontiersin.org/articles/10.3389/frans.2023.1118749 https://www.frontiersin.org/articles/10.3389/frans.2023.1118749 2023-05-18T00:00:00Z Review Prashant N. JethvaMichael L. Gross https://www.frontiersin.org/articles/10.3389/frans.2023.1119438 https://www.frontiersin.org/articles/10.3389/frans.2023.1119438 2023-03-31T00:00:00Z Mini Review Lucas C. LazariGilberto Santos de OliveiraJanaina Macedo-Da-SilvaLivia Rosa-FernandesGiuseppe Palmisano https://www.frontiersin.org/articles/10.3389/frans.2023.1157582 https://www.frontiersin.org/articles/10.3389/frans.2023.1157582 2023-03-23T00:00:00Z Mini Review Dominik Schwudke https://www.frontiersin.org/articles/10.3389/frans.2023.1142606 https://www.frontiersin.org/articles/10.3389/frans.2023.1142606 2023-02-24T00:00:00Z Mini Review Audrey Le GouellecCaroline PlazyBertrand Toussaint https://www.frontiersin.org/articles/10.3389/frans.2023.1119489 https://www.frontiersin.org/articles/10.3389/frans.2023.1119489 2023-02-24T00:00:00Z Perspective Jonathan SchulteJan-Niklas TantsJulian von EhrAndreas SchlundtNina Morgner https://www.frontiersin.org/articles/10.3389/frans.2023.1112390 https://www.frontiersin.org/articles/10.3389/frans.2023.1112390 2023-02-13T00:00:00Z Perspective Julien BoccardSerge Rudaz https://www.frontiersin.org/articles/10.3389/frans.2023.1118494 https://www.frontiersin.org/articles/10.3389/frans.2023.1118494 2023-02-13T00:00:00Z Perspective Valentina CalabreseArnaud SalvadorYohann ClémentThomas Alexandre BrunetAnabelle EspeyteArnaud ChaumotOlivier GeffardDavide Degli-EspostiSophie Ayciriex https://www.frontiersin.org/articles/10.3389/frans.2023.1118742 https://www.frontiersin.org/articles/10.3389/frans.2023.1118742 2023-02-10T00:00:00Z Perspective Marie ValmoriVincent MarieFrançois FenailleBenoit ColschDavid Touboul