AUTHOR=Du Jiagang , Zhuang Rufei , Zhang Yuanju , Wang Yan , Liu Yunguo , Zhang Qianqian , Zhang Jianying TITLE=Dietary metabolic energy/lysine ratios improve the yolk color and Hastelloy unit without affecting the egg performance of brown laying hens JOURNAL=Frontiers in Animal Science VOLUME=Volume 6 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/animal-science/articles/10.3389/fanim.2025.1638293 DOI=10.3389/fanim.2025.1638293 ISSN=2673-6225 ABSTRACT=This experiment evaluated the impact of varying ratios of dietary metabolic energy: lysine (ME: Lys) in the diet on egg production, egg quality, and feed intake of laying hens. A total of 432 Hy-line brown laying hens, aged 55 wk, were reared over a period of 11 weeks. Hens were individually assigned to a basal standard diet with a ME to Lys ratio of 275:64 kcal/mg, along with five additional ratio types: 270:74 kcal/mg, 275:74 kcal/mg, 280:74 kcal/mg, 285:74 kcal/mg, and 290:74 kcal/mg. Feed and fecal digestibility were determined and egg quality were assayed on wk 1, 5, 9 and 11. The results indicated that an ME/Lys ratio of 285:74 kcal/mg improved yolk color pigment during weeks 9 and 11. Additionally, a ratio of 290:74 kcal/mg contributed to higher Hastelloy unit measurements during weeks 5, 9, and 11. In summary, while different ME/Lys ratios had no impact on overall egg production, both the 285:74 and 290:74 kcal/mg ratios demonstrated positive effects on deeper yolk color pigment and higher Hastelloy unit values.