AUTHOR=Ge Chengfeng , Chen Haiqin , Mei Tiantian , Tang Xin , Chang Lulu , Gu Zhennan , Zhang Hao , Chen Wei , Chen Yong Q. 

TITLE=Application of a ω-3 Desaturase with an Arachidonic Acid Preference to Eicosapentaenoic Acid Production in Mortierella alpina

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 5 - 2017

YEAR=2018

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2017.00089

DOI=10.3389/fbioe.2017.00089

ISSN=2296-4185

ABSTRACT=In the industrial oleaginous fungus Mortierella alpina, the arachidonic acid (AA; C20:4; ω-6) fraction can reach 50% of the total fatty acids in vivo. However, the eicosapentaenoic acid (EPA; C20:5; ω-3) fraction is less than 3% when this fungus is cultivated at a low temperature (12°C). Omega-3 fatty acid desaturase is a key enzyme in ω-3 long-chain polyunsaturated fatty acids (LC-PUFAs) biosynthesis pathways. To enhance EPA production, we transformed the ω-3 fatty acid desaturase (PaD17), which exhibits strong Δ-17 desaturase activity, into Mortierella alpina, thus increasing the AA to EPA conversion rate to 49.8%. This PaD17-harbouring Mortierella alpina reconstruction strain produced 617 mg L-1 of EPA at room temperature in broth medium, this yield was increased to 1.73 g L-1 after culture medium optimisation, (i.e., about three fold higher than that under original culture conditions), with concomitant respective increases in dry cell weight and total fatty acids content to 16.55 g L-1 and 6.46 g L-1. These findings suggest a new platform for the future industrial production of EPA.