AUTHOR=Janjić Klara , Samiei Mohammad , Moritz Andreas , Agis Hermann 

TITLE=The Influence of Pro-Inflammatory Factors on Sclerostin and Dickkopf-1 Production in Human Dental Pulp Cells Under Hypoxic Conditions

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 7 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00430

DOI=10.3389/fbioe.2019.00430

ISSN=2296-4185

ABSTRACT=Sclerostin (Sost) and dickkopf (Dkk)-1 are inhibitors of the Wnt signalling pathway that plays a role in regenerative processes. Hypoxia-based strategies are used for regenerative approaches, but the influence of hypoxia on Sost and Dkk-1 production in a pro-inflammatory environment is unclear. The aim of this study was to assess if a pro-inflammatory has a different influence on Sost and Dkk-1 production in dental pulp cells (DPC) under normoxia than under hypoxia. Human DPC were treated with interleukin (IL)-1β, tumor necrosis factor (TNF)α or transforming growth factor (TGF)β, with L-mimosine (L-MIM) or hypoxia or a combination. Sost and Dkk-1 mRNA levels were measured with qPCR. TNFα, TGFβ, L-MIM or combined treatment do not modulate Sost and Dkk-1. IL-1β downregulates Sost. Hypoxia alone and together with inflammatory markers downregulates Dkk-1. In conclusion, there is a differential effect of hypoxia and IL-1 β on the production of Dkk-1 and Sost.