AUTHOR=Xiao Qiuyun , Liu Zihan , Zhao Xuan , Xiong Hai 

TITLE=Multiple Site-Specific One-Pot Synthesis of Two Proteins by the Bio-Orthogonal Flexizyme System

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 8 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00037

DOI=10.3389/fbioe.2020.00037

ISSN=2296-4185

ABSTRACT=Acetylation of lysine is a reversible post-translational modifications (PTMs), which plays a key role in regulating gene expression and dynamic transitions in chromatin remodeling. Here, we have developed the first one-pot bio-orthogonal Flexizyme systems: the acetyl-lysine (AcK) and non-hydrolyzable thioacetyl-lysine (ThioAcK) were site-specifically incorporated into human histone H3 and H4 at different lysine positions in vitro, either individually or in pairs. In this study, we demonstrated its high efficiency and accuracy toward one-pot synthesis of two desired histone variants.