AUTHOR=Lanna Mariana Ferreira , Resende Lucilene Aparecida , Aguiar-Soares Rodrigo Dian de Oliveira , de Miranda Marina Barcelos , de Mendonça Ludmila Zanandreis , Melo Júnior Otoni Alves de Oliveira , Mariano Reysla Maria da Silveira , Leite Jaqueline Costa , Silveira Patricia , Corrêa-Oliveira Rodrigo , Dutra Walderez Ornelas , Reis Alexandre Barbosa , Martins-Filho Olindo Assis , de Moura Sandra Aparecida Lima , Silveira-Lemos Denise , Giunchetti Rodolfo Cordeiro 

TITLE=Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 8 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.538203

DOI=10.3389/fbioe.2020.538203

ISSN=2296-4185

ABSTRACT=The characterization of immune events in sponge implants would be useful to identify the immunological events that could support the selection of optimized protocols for novel applications of this model. Here, the changes in histological/morphometric, immunophenotypic and functional features of infiltrating leukocytes were assessed in sponge implants for Swiss, BALB/c, and C57BL/6 mice. A gradual increase of fibrovascular stroma and a progressive decrease in leukocyte infiltration, mainly composed of polymorphonuclear cells with progressive shift towards mononuclear cells at late time-points, were observed over time. Usually, Swiss mice presented more prominent immune response with late mixed pattern of cytokine production. While BALB/c mice showed an early activation of the innate response with a controlled cytokine profile, C57BL/6 mice presented a typical early pro-inflammatory response with persistent neutrophilic involvement. A rational selection of the ideal time-point/mouse-lineage would avoid bias or tendentious results. Criteria such as: low number of increased biomarkers, no recruitment of cytotoxic response, minor cytokine production and lower biomarker connectivity guided the choice of the best time-point for each model (Day5/Swiss; Day7/BALB/c; Day6/C57BL/6) with wide application for screening purposes, such as identification of therapeutic biomolecules, selection of antigens/adjuvants, and follow-up of innate and adaptive immune response to vaccines candidates.