AUTHOR=Chung Christina Z. , Amikura Kazuaki , Söll Dieter 

TITLE=Using Genetic Code Expansion for Protein Biochemical Studies

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 8 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.598577

DOI=10.3389/fbioe.2020.598577

ISSN=2296-4185

ABSTRACT=Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of noncanonical amino acids into proteins. By taking advantage of the unique properties of noncanonical amino acids, we can identify spatiotemporal-specific protein states within living cells. Insertion of more than one noncanonical amino acid allows for selective labeling that can aid in the identification of weak or transient protein-protein interactions. This review will discuss recent studies applying genetic code expansion for protein labeling and identifying protein-protein interactions, and offer considerations for future work in expanding genetic code expansion methods.