AUTHOR=Chen Wancheng , Yuan Sijie , Shen Jie , Chen Yongsheng , Xiao Yang TITLE=A Composite Hydrogel Based on Pectin/Cellulose via Chemical Cross-Linking for Hemorrhage JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=Volume 8 - 2020 YEAR=2021 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.627351 DOI=10.3389/fbioe.2020.627351 ISSN=2296-4185 ABSTRACT=Hydrogel-based material has been demonstrated promising potential for hemostasis. Herein, we prepared a composite hydrogel (CH-P 40%) by combining pectin and cellulose in ionic liquid. The superficial morphology of the CH-P 40% was explored by SEM; the internal chemical bonds, crystal form and thermal stability were determined via FTIR, XRD and thermogravimetric analysis, respectively. The biocompatibilities of CH-P 40% hydrogel were evaluated by MTT, flow cytometry, and by histological observation of H&E staining. Furthermore, the hemostatic effect was evaluated via blood clotting index and mouse liver hemostatic model. Shown as the results, the CH-P 40% hydrogel performed a dense network structure and remained the chemical bonds including OH, CH, C = O, -CH2, CO, C1-H and β-glycosidic. Simultaneously, the hydrogel reserved Cellulose I and II crystal structure and favorable thermal stability. Moreover, the CH-P 40% treated cell proliferation rates both increased (P > 0.05) and there were no pathological lesions in mouse organs, which suggesting favorable biocompatibility. The results showed the lower amount of bleeding in the hydrogel-treated liver wound within 3 minutes. Overall, the pectin-cellulose hydrogel is stable, and possesses favorable biocompatibility and hemostatic ability, further highlighting the composite hydrogel has the potential capability to be rapid hemostasis biomedical material.