AUTHOR=Zhang Yuhan , Wang Ting , Li Juan , Cui Xiaoming , Jiang Mingxia , Zhang Mogen , Wang Xiaoli , Zhang Weifen , Liu Zhijun 

TITLE=Bilayer Membrane Composed of Mineralized Collagen and Chitosan Cast Film Coated With Berberine-Loaded PCL/PVP Electrospun Nanofiber Promotes Bone Regeneration

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.684335

DOI=10.3389/fbioe.2021.684335

ISSN=2296-4185

ABSTRACT=The study involved developing a bilayer membrane that provides good bioactivity and mechanical characteristics by combining mineralized collagen (MC) powder and berberine. The bilayer membrane was prepared via coating Polycaprolactone (PCL)/Polyvinylpyrrolidone (PVP) nanofibers which load with berberine (BER)  on mineralized collagen (MC) and chitosan (CS) cast film. Electrospinning nanofibers membrane has a three-dimensional structure, the fibers are uniform diameter, and have improved hydrophilicity. The bilayer membrane showed suitable attachment and proliferation of osteoblasts, superior mechanical properties, and promoted bone regeneration in rats’ femur with the best effect. This work demonstrates a promising way to address the regeneration of bone defects by using the BER@PCL/PVP-MC/CS bilayer membrane.