AUTHOR=Li Wenqiang , Jian Xingling , Zou Yanfen , Wu Lin , Huang Haiyan , Li Hui , Hu Dandan , Yu Bo 

TITLE=The Fabrication of a Gellan Gum-Based Hydrogel Loaded With Magnesium Ions for the Synergistic Promotion of Skin Wound Healing

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.709679

DOI=10.3389/fbioe.2021.709679

ISSN=2296-4185

ABSTRACT=In order to accelerate the serious skin burn wound healing in convenient manner, an interpenetrating network hydrogel consisting of gellan gum and polyacrylamide was synthesized by chemical crosslinking and Mg2+ ions immersion techniques. The prepared Mg2+@PAM/GG hydrogel was characterized by morphology, water vapour loss, swelling ratio, rheological properties, tensile mechanical, biocompatibility and flow cytometry study. The results showed that Mg2+@PAM/GG hydrogel mechanical strength could be enhanced by the dual network structure and physical crosslinking agent Mg2+ ions. In addition, the tension strength and of Mg2+@PAM/GG hydrogel is obviously increased from 86 to 392 KPa, the elongation at break increased from 84% to 231% and crosslinking density N increased from 4.3 to 7.2 mol/m3 compared to the pure GG hydrogel. The cumulative release curve of Mg2+ ions showed that the multiple release mechanism of Mg2+ ions and belong to non-Fick’s diffusion. Meanwhile, in vitro experiments show that Mg2+@PAM/GG double network hydrogel has favorable proliferation and NF-κB pathway inhibition property for fibroblast cells. Finally, the healing effect of the Mg2+@PAM/GG was evaluated in rat full-thickness burns model. The animal study demonstrates that Mg2+@PAM/GG could accelerate the healing efficiency in cause of the sustained-released Mg2+ ions into wound beds. Considering these excellent performance, this convenient prepared hydrogel has great potential commercial application for skin full-thickness burns healing materials.