AUTHOR=Liu Yanchang , Cheng Wendan , Zhao Yao , Gao Liang , Chang Yongyun , Tong Zhicheng , Li Huiwu , Jing Juehua 

TITLE=Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.735949

DOI=10.3389/fbioe.2021.735949

ISSN=2296-4185

ABSTRACT=Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extraordinarily sensitive to external mechanical signals. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSCs, which provide the integration of host bone and prosthesis benefits. But the mechanism is still unclear. In this study, BMSCs planted on the surface of TiO2 nanotubes were subjected to cyclic mechanical stress and related mechanisms were explored. The results of ALP staining, real-time PCR, Western blot showed that cyclic mechanical stress can regulate the expression level of osteogenic differentiation markers in BMSCs on the surface of TiO2 nanotubes through Wnt/β-catenin. As an important member of histone acetyltransferase family, GCN5 exerted regulatory effects on receiving mechanical signals. The results of ChIP assay indicated that GCN5 could activate the Wnt promoter region. Hence, we concluded that the osteogenic differentiation ability of BMSCs on the surface of TiO2 nanotubes was enhanced under the stimulation of cyclic mechanical stress and GCN5 mediated this process through Wnt/β-catenin.