AUTHOR=Wang Hao , Wang Lin , Zhong Baihua , Dai Zhuojun 

TITLE=Protein Splicing of Inteins: A Powerful Tool in Synthetic Biology

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.810180

DOI=10.3389/fbioe.2022.810180

ISSN=2296-4185

ABSTRACT=Inteins are protein segments that are capable of enabling the ligation of flanking extein into a new protein, a process known as protein splicing. Since its discovery, inteins have become powerful biotechnological tools for applications such as protein engineering. In the last ten years, the development in the synthetic biology has further endow inteins with enhanced functions and diverse utilizations. Here we review these efforts.