AUTHOR=Yasmin Azra , Aslam Fozia , Fariq Anila 

TITLE=Genetic Evidences of Biosurfactant Production in Two Bacillus subtilis Strains MB415 and MB418 Isolated From Oil Contaminated Soil

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.855762

DOI=10.3389/fbioe.2022.855762

ISSN=2296-4185

ABSTRACT=Biosurfactants are diverse group of amphiphilic compounds obtained from microbes. In present study genomic analysis of biosurfactant producing Bacillus subtilis MB415 and MB418 obtained from oil contaminated soil was performed. Initially strains were screened for biosurfactant production by hemolytic assay, emulsification index and oil displacement. Further FTIR analysis of extracted biosurfactants revealed the presence of lipopeptides. Sequenced genomes of MB415, MB418 were of 4.2Mbps with 43% GC content. Among more than 4500 protein coding genes, many were involved in virulence, metal/ multidrug resistances, flagella assembly, chemotactic response, aromatic ring hydroxylating dioxygenases. Annotation analysis revealed both genomes possessed non-ribosomal synthetase gene clusters for lipopeptide synthetase srf & fen responsible for surfactin and fengycin production. Comparative studies of both genomes highlighted variability in gene operons mainly for surfactin biosynthesis.