AUTHOR=Zhang Shang , Gao Lichang , Wang Pin , Ma Yuyan , Wang Xiaoliang , Wen Jie , Cheng Yu , Liu Changlin , Zhang Chunxia , Liu Changfeng , Yan Yongli , Zhao Chengru 

TITLE=A minimally manipulated preservation and virus inactivation method for amnion/chorion

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.952498

DOI=10.3389/fbioe.2022.952498

ISSN=2296-4185

ABSTRACT=Allogeneic amnion tissues have been widely used in tissue repair and regeneration, especially a remarkable trend of clinical uses in chronic wound repair. The virus inactivation procedures are necessary and required to be verified for the approval of biological products. Cobalt-60 (Co-60) or electron-beam (e-beam) are the common procedures for virus and bacterial reduction but the excessive dose of irradiation was reported to be harmful for biological products. Herein we present a Riboflavin (RB)-Ultraviolet Light (UV) method for virus inactivation of amnion and chorion tissues. We used the standard in vitro limiting dilution assay to test the viral reduction capacity of the RB-UV method on amnion or chorion tissues loaded with four types of model viruses. We found RB-UV was a very effective procedure of inactivating viruses of amnion and chorion tissues, which could be used as a complementary way of Co-60 irradiation. In addition, we also screened the washing solutions and drying methods for the retention of growth factors.