AUTHOR=Chen Jianfu , Yang Yuan , Li Yunyan , Xu Lirong , Zhao Chun , Chen Qi , Lu Yongping 

TITLE=Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1163405

DOI=10.3389/fbioe.2023.1163405

ISSN=2296-4185

ABSTRACT=Introduction: The side effects of conventional therapy for acute deep vein thrombosis (DVT) are severe, and inflammatory reactions play a pivotal role in thrombosis. It is particularly important to explore new ways of thrombus treatment by targeting inflammatory factors.
Methods: Targeted microbubble contrast agent was prepared by biotin-avidin method. The 40 DVT model rabbits were established and divided into 4 groups according to different treatment regimens. The four coagulation indexes, TNF-α and D-dimer content of experimental animals were measured before modeling, before and after treatment, and the thrombolysis was assessed by ultrasound imaging. Finally, the results were verified by pathology.
Results  and Discussion: Fluorescence microscopy verified successful preparation of targeted microbubbles. Comparison among groups: PT, APTT and TT in Group II-IV were longer than those in Group I (all p < 0.05). FIB and D-dimer content were lower than those in Group I (all p < 0.05), and TNF-α content in Group IV was lower than that in Group I-III (all p < 0.05). Pairwise comparison before modeling, before treatment and after treatment: After treatment, the PT, APTT and TT in Group II-IV were longer than those before modeling (all p < 0.05). The contents of FIB and D-dimer were lower than those before modeling and before treatment (all p < 0.05), the content of TNF-α decreased significantly only in Group IV, but increased in the other three groups. targeted microbubbles combined with Low-power focused ultrasound can reduce inflammation, significantly promote thrombolysis, and provide new ideas and methods for the diagnosis and treatment of acute DVT.