AUTHOR=Oliva Stefano , Diomede Francesca , Della Rocca Ylenia , Mazzone Antonella , Marconi Guya Diletta , Pizzicannella Jacopo , Trubiani Oriana , Murmura Giovanna 

TITLE=Anti-TLR4 biological response to titanium nitride-coated dental implants: anti-inflammatory response and extracellular matrix synthesis

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1266799

DOI=10.3389/fbioe.2023.1266799

ISSN=2296-4185

ABSTRACT=Osteointegration is a key process during dental implant placement and it is related to titanium surface topography. Implant coating and surface modification methods are able to ameliorate the bone production and the osteogenic process. The current work aimed at evaluating the biological outcomes of two different surface of dental implants, machined and Titanium Nitride (TiN) coated, at inflammation level using an in vitro model of human periodontal ligament stem cells (hPDLSCs). The TLR4/MyD88/NFκB p65/NLRP3 pathway induced by the P. Gingivalis lipopolysaccharide (LPS-G) was studied by means of gene and protein level expression. Moreover, expression of Vimentin, Vinculin and Fibronectin was evaluated to investigate the effects on the cells adhesion and extracellular matrix (ECM) deposition. The results of the present study suggest that TiN coated titanium disks may modulate the inflammation by the suppression of TLR4/MyD88/NFκB p65/NLRP3 pathway and accelerate ECM apposition.