AUTHOR=Xiao Yubei , Tan Xuemei , He Qiaoning , Yang Shihui 

TITLE=Systematic metabolic engineering of Zymomonas mobilis for β-farnesene production

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 12 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2024.1392556

DOI=10.3389/fbioe.2024.1392556

ISSN=2296-4185

ABSTRACT=1. Five β-farnesene synthases screened with AaBFS selected for β-farnesene production in Zymomonas mobilis.2. β-farnesene production improved by increasing the expression of AaBFS using strong promoter or multiple copies through multiple-plasmid constructs or genomic multi-locus integration.3. MEP flux enhancement crucial for increasing β-farnesene production by enhancing the expression of key enzymes of DXS, IspG, and IspH. 4. β-farnesene production improved by optimizing aeration and C/N ratios.5. The highest β-farnesene titer of 159.7 mg/L achieved for recombinant Z. mobilis in flask fermentation.