AUTHOR=Xie Qi , Wang Tianqi , He Lina , Liang Hongbo , Sun Jingxuan , Huang Xiaoxiao , Xie Weili , Niu Yumei 

TITLE=Biological and structural properties of curcumin-loaded graphene oxide incorporated collagen as composite scaffold for bone regeneration

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 12 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2024.1505102

DOI=10.3389/fbioe.2024.1505102

ISSN=2296-4185

ABSTRACT=To address the challenges related to bone defects, including osteoinductivity deficiency and postimplantation infection risk, this study developed the collagen composite scaffolds (CUR-GO-COL) with multifunctionality by integrating the curcumin-loaded graphene oxide with collagen through a freeze-drying-cross-linking process. Their morphological and structural characteristics were analyzed, as well as the physicochemical properties, including water absorption, water retention and porosity. The CUR-GO-COL composite scaffold with a CUR-GO concentration of 0.05% (w/v) exhibits optimal biological compatibility and stable and slow curcumin release rate. Furthermore, in vitro cell and bacterial tests demonstrated that the prepared CUR-GO-COL composite scaffolds enhance cell viability, proliferation and adhesion, and offer superior osteogenic and antimicrobial properties compared with the CUR-GO composite scaffold, confirming the osteogenesis promotion and antimicrobial effects. Therefore, the introduction of CUR-GO into collagen scaffold creates a bonefriendly microenvironment, and offers a theoretical foundation for the design, investigation and utilization of multifunctional bone tissue biomaterials.