AUTHOR=Casarella Simona , Ferla Federica , Di Francesco Dalila , Pagani Veronica , Di Varsavia Carolina , Regano Irene , Boccafoschi Francesca 

TITLE=Laminin-derived peptide drives the cardiomyogenic potential and cardiac cells functionality

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 13 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1629412

DOI=10.3389/fbioe.2025.1629412

ISSN=2296-4185

ABSTRACT=Laminin represents a major component of the basement membrane in cardiac tissue. Through integrins binding, laminin can sustain cell adhesion, proliferation, differentiation and mechanotransduction. The role of a small bioactive peptide (KKGSYNNIVVHV) derived from laminin in cardiac differentiation and functionality has been demonstrated. Briefly, results showed how the presence of laminin-derived peptide enhanced the differentiation of mesenchymal stem cells into cardiomyocytes-like phenotype, by modulating not only the cytoskeletal apparatus but also enhancing the cardiac and adhesion markers. Moreover, neonatal mouse cardiomyocytes in presence of the peptide showed well organized cytoskeletal rearrangements and regulated contractility. Therefore, in this study the role of laminin-derived peptide in guiding cardiomyogenesis has been characterized, opening innovative approaches in functional cardiac tissue engineering and regenerative medicine.