Abstract
Background:
Figs (Ficus carica L.) are rich in sugars and bound aroma precursors that can be converted into aroma compounds.
Methods:
In this study, the W-8 strain was isolated from fig epidermis and identified as Torulaspora delbrueckii by 26S rDNA sequencing. By optimizing fermentation conditions using single-factor experiments and an orthogonal design, aroma compound (AC) production was evaluated. The whole genome of strain W-8 was sequenced and annotated.
Results:
AC production was 1.64 times that of the control. Isoamyl alcohol (0.264 mg/mL), phenethyl alcohol (0.053 mg/mL), isoamyl acetate (0.006 mg/mL), isobutyric acid (0.012 mg/mL), and 2-methylbutyric acid (0.004 mg/mL) were exclusively produced, and production of ethyl stearate and acetoin was increased by 4.20-fold and 2.30-fold, respectively. Strain W-8 harbors a complete genome of 9,224,546 bp. KEGG analysis showed prominent representation of amino acid and carbohydrate metabolism pathways, while GO annotation indicated enrichment in catalytic and metabolic processes. CAZy analysis identified key carbohydrate-active enzymes, including β-glucosidase.
Conclusion:
Genome analysis showed that T. delbrueckii W-8 possesses β-glucosidases and aroma-related metabolic pathways, providing a genetic basis for the release of bound aroma precursors from fig substrates and their conversion into AC. These findings highlight T. delbrueckii W-8 as a promising starter for enhancing aroma quality in fermented fig products.
1 Introduction
Natural aroma substances are increasingly favored by consumers compared with synthetic flavor substances and therefore drive the demand for the sustainable production of these compounds (; Melini and Melini, 2024). Microbial fermentation has great potential for the production of various natural aroma compounds, including higher alcohols, esters, organic acids, and other volatile substances through various metabolic pathways, such as the Ehrlich pathway (; ; Saerens et al., 2010). The precursors of these aroma compounds are found in many fruits in glycosidically bound forms (; Schober et al., 2023).
Ficus carica L. (fig) is rich in fermentable sugars, organic acids, phenolic acids, flavonoids, and other bioactive compounds (; ; Oliveira et al., 2010), making it a promising substrate for the production of aroma compounds through microbial fermentation. Microbial fermentation can break down the cell walls of figs, releasing more aroma compounds. Meanwhile, the metabolites produced by microorganisms themselves can increase the variety of aroma compounds in figs and enhance their aromatic quality.
Non-Saccharomyces yeasts have received extensive attention because of their ability to produce aroma compounds during the fermentation of different substrates (Padilla et al., 2016; Varela, 2016). T. delbrueckii produces low volatile acids, can significantly promote the release of aroma compounds, and generate floral, fruity, caramel, and other characteristic aromas (; ; Silva-Sousa et al., 2022). It is noteworthy that T. delbrueckii expresses β-glucosidase, which can hydrolyze glycoside-bound aroma precursors into free aroma compounds (; Schober et al., 2023). Thus, it is regarded as having potential application value. At the genomic level, recent studies have improved the genome annotation of T. delbrueckii and have shown that it is significantly enriched in metabolic functions related to fermentation performance (Santiago et al., 2021; Silva-Sousa et al., 2024).
However, at present, most research on T. delbrueckii focuses on wine and beer fermentation (; Silva-Sousa et al., 2022; Zhang et al., 2018). Existing research on fig fermentation mostly uses Saccharomyces cerevisiae or lactic acid bacteria (Ma and Liu, 2010; Wang et al., 2023), and fermentation of figs using non-Saccharomyces yeasts for aroma production has not yet been reported.
In this study, a strain of T. delbrueckii (W-8) was isolated from fig surfaces and applied to produce aroma compounds through fig fermentation. The fermentation conditions were optimized using single-factor experiments and orthogonal design to achieve higher yield of aroma compounds. Therefore, the primary objective of this study is to utilize microbial fermentation as a biotechnological process to efficiently release and synthesize natural aroma compounds from figs. These volatile metabolites hold great potential as natural flavor extracts for the food and fragrance industries. Additionally, the whole genome of strain W-8 was sequenced and annotated using the KEGG, GO, and CAZy databases to predict key aroma-related enzymes and metabolic pathways.
2 Materials and methods
2.1 Screening of microorganisms from fig for enhancing aroma quality
Fresh figs used for yeast isolation were purchased from Dazhang Supermarket, Zhengzhou, China, while dried figs were obtained from Aksu Prefecture, Xinjiang, China. The epidermis of both fresh and dried figs was aseptically cut using a sterile scalpel, then fig tissue (10 g) was transferred into 90 mL of sterile water and mixed for 1 h at 30 °C and 180 rpm on a shaker (ZWYR-2102C, Shanghai Zhicheng). Subsequently, 5% (v/v) of the suspension was inoculated into YPD liquid medium and incubated at 28 °C and 150 rpm for 48 h in a shaking incubator. Afterwards, 100 μL of 10−3–10−5 dilutions were spread on YPD agar plates and incubated at 30 °C for 48 h in an incubator (DH-400, Beijing Zhongxing Weiye). The microbial colonies were examined under a light microscope. Colonies exhibiting typical yeast-like morphological features, such as oval or spherical cell shapes and signs of budding reproduction, were selected and cultured in YPD liquid medium. The isolates were streaked on fig solid medium (prepared by adding 2% w/v agar to the fig extract broth) for purification. For secondary screening, overnight cultures were inoculated (3%, v/v) into 30 mL fig fermentation medium (Powdered dried figs) prepared from 10 g dried figs per 100 mL water and incubated at 28 °C with shaking at 150 rpm for 36 h.
2.2 Sensory evaluation
Sensory evaluation was performed on the fermentation broth using fig as the fermentation substrate. Strains that significantly enhanced the aroma compounds of fig after fermentation were screened based on sensory evaluation scores. After fermentation, 10 mL of the fermentation broth was collected for odor evaluation. Quantitative descriptive analysis was conducted in accordance with GB/T 10220-2012 (Sensory analysis—Methodology—General guidelines), and eight trained sensory panelists with a background in food science evaluated the aroma attributes. The sensory analysis was carried out at room temperature (25 °C), and five attributes—floral, fruity, wine-like, fresh, and sweet—were assessed on an intensity scale ranging from 0 to 5. The final sensory scores were expressed as the mean values of the eight panelists.
2.3 DNA extraction and 26S rDNA sequencing of aroma-producing yeast
Genomic DNA from the W-8 strain was extracted from an overnight YPD culture by using the Yeast Genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). DNA quality and concentration were assessed using a NanoDrop spectrophotometer by measuring the A260/A280 ratio. The genomic DNA was submitted to Sangon Biotech (Shanghai) Co., Ltd. at 30 ng/μL for 26S rDNA sequencing. The D1/D2 domain of the 26S/28S rDNA gene was amplified using universal yeast primers: NL1: 5′-GCATATCAATAAGCGGAGGAAAAG-3′ and NL4: 5′-GGTCCGTGTTTCAAGACGG-3′. The obtained sequences were compared with homologous sequences in the NCBI database using BLAST, and a phylogenetic tree was constructed in MEGA11.
2.4 Surface morphology of aroma-producing yeast
For SEM analysis, 1 mL of W-8 culture was harvested from the fermentation medium and centrifuged at 5,000 × g for 5 min. The pellet was washed with phosphate-buffered saline, fixed in 2.5% glutaraldehyde, and dehydrated through a graded ethanol series (30%–99%, 1 mL each step). The dehydrated cells were air-dried on aluminium stubs mounted with conductive adhesive, sputter-coated with a thin gold layer, and observed by SEM following previously described protocols (; Watson et al., 1980).
2.5 GC–MS analysis of volatile compounds in fermented fig broth
In this study, the fermented fig broth without yeast inoculation was used as a blank control. To extract volatile compounds, the 10 mL fermentation broth was mixed with 15 mL dichloromethane, vortexed for 10 min, and centrifuged at 10,000 rpm for 5 min to separate the organic phase. The extraction was repeated three times. The combined organic extracts were dried overnight using anhydrous sodium sulphate and concentrated to 1 mL. The 2,6-dichlorotoluene (0.5211 mg/mL) was added to the concentrated extract and membrane-filtered (0.22 μm) before GC–MS analysis.
GC–MS analysis was performed using an Agilent 8890 GC coupled with a 5977B mass spectrometer equipped with a DB-5MS capillary column. The injector temperature was set at 280 °C, with a split ratio of 3:1. Helium was used as the carrier gas at a flow rate of 1.0 mL/min. The oven temperature program was as follows: initial temperature 50 °C held for 3 min, increased to 130 °C at 2 °C/min and held for 3 min, then raised to 180 °C at 5 °C/min and held for 2 min, and finally increased to 280 °C at 3 °C/min and held for 5 min. Mass spectrometry was conducted in electron impact (EI+) mode at 70 eV, with the ion source temperature maintained at 230 °C ± 1 °C and the quadrupole temperature at 150 °C. Data acquisition was initiated 7 min after injection to avoid solvent interference, and spectra were recorded in full scan mode (m/z 35–500). Volatile compounds were identified by comparison with the NIST 20 mass spectral library using a match threshold of ≥70%, and semi-quantification was performed using the 2,6-dichlorotoluene internal standard. The total content of aroma compounds was calculated using the internal standard method, defined as the ratio of the total peak area of all aroma compounds to the peak area of the internal standard, multiplied by the concentration of the internal standard, to yield the total aroma content (mg/mL).
2.6 Optimization of fermentation conditions for aroma-producing yeast strain
A large single colony of the W-8 strain was inoculated into YPD liquid medium and cultured for 36 h under the growth conditions mentioned above. Optical density at 600 nm was measured every 4 h, and a growth curve was constructed. The fermentation process parameters were optimized through single-factor experiments followed by an orthogonal experiment (Table 1). Fermentation time, fermentation temperature, and initial pH were selected as the main factors affecting aroma production. The relative content of aroma compounds (%)in the fermentation broth were used as the evaluation index. The relative content of aroma compounds (%) was calculated as the ratio of the total volatile compound concentration under a specific fermentation condition to the maximum total volatile compound concentration achieved among all tested conditions in that single-factor experiment.
TABLE 1
| Level | Factor | ||
|---|---|---|---|
| A fermentation time (h) | B fermentation temperature (°C) | C initial pH value | |
| −1 | 24 | 28 | 6.0 |
| 0 | 36 | 30 | 7.0 |
| 1 | 48 | 32 | 8.0 |
Factors and levels for the orthogonal experiment design.
Single-factor experiments were conducted to evaluate the effects of fermentation time, temperature, and initial pH. For fermentation time optimization, cultures were incubated at 30 °C, 150 rpm, and an initial pH of 5, and samples were collected at 12, 24, 36, 48, and 60 h. Based on the optimal fermentation time, the effect of temperature was examined by cultivating the strain at 25, 28, 30, 32, and 35 °C under the same pH and shaking conditions. Subsequently, the effect of initial pH was evaluated at the optimized time and temperature by adjusting the medium pH to 5, 6, 7, 8, and 9, while keeping all other parameters constant.
2.7 Whole-genome sequencing of Torulaspora delbrueckii W-8
2.7.1 Sample preparation for sequencing
Strain W-8 was grown in YPD medium for 24 h, and genomic DNA was extracted using a yeast genomic DNA extraction kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s instructions. DNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, United States) by measuring the A260/A280 ratio. The extracted genomic DNA was stored at −20 °C until sequencing. Whole-genome sequencing was performed by Sangon Biotech (Shanghai) Co., Ltd.
DNA extracted from T. delbrueckii W-8 was used to construct the library. Based on concentration and purity qualifications, 20 kb fragment libraries were constructed. De novo sequencing of the yeast genome was performed using second-generation Illumina HiSeq technology. Raw sequencing data were stored in FASTQ format, and quality control was performed to remove low-quality reads and adapters, yielding high-quality, clean data for downstream analysis.
2.7.2 Gene prediction and functional annotation
Protein-coding genes in the fungal genome were predicted using GeneMark-ES/ET software (; Ter-Hovhannisyan et al., 2008). Sequence alignment of predicted protein-coding genes against the KEGG database () was performed using Diamond software with an E-value cutoff of <1e−5 (). Functional annotation against the GO database () was conducted using BLASTP, with the sequence alignment threshold set to E-value ≤ 1e−5. Prediction of CAZy enzyme genes was performed using the hmmscan software (), selecting sequences with E-value <1e−5 for ORF lengths >80 amino acids, E-value <1e−3 for alignment lengths <80 amino acids, and where the alignment length exceeded 30% of the reference sequence length ().
3 Results and discussion
3.1 Screening of aroma-producing non-canonical yeast from fig
3.1.1 Screening and sensory selection of candidate strains
Initially, 20 yeast isolates were obtained from the fig samples. During the secondary screening, these isolates were evaluated based on sensory scoring. Ultimately, three strains exhibiting the most pronounced aroma intensities were selected and purified for further analysis.
To systematically evaluate the impact of different strains on the sensory quality of the fermented product, quantitative descriptive analysis (QDA) was performed across five core aroma attributes: floral, fruity, sweet, fresh, and winey notes. The non-inoculated control group served as the sensory baseline, with all attributes uniformly scored at 2.0. As visualized in the radar chart (Figure 1), all tested strains significantly improved the overall sensory profile relative to the control, with distinct strain-specific patterns of aroma enhancement observed. Strain W-8 showed the strongest overall aroma performance, with scores of 4.3 for floral and fruity attributes, 3.3 for sweet and fresh attributes, and 3.0 for the winey attribute. Strain W-6 displayed moderate enhancement, with scores of 3.8, 3.3, 3.0, 2.9, and 2.4 for floral, fruity, sweet, fresh, and winey attributes, respectively. Strain W-4 also improved the aroma profile and showed a relatively stronger sweet attribute (3.7).
FIGURE 1
These sensory characteristics are typically associated with higher alcohols and ester compounds, such as phenethyl alcohol, isoamyl acetate, and ethyl esters, which are known to contribute floral and fruity aromas in fermented products (Yan et al., 2019). Similar sensory-guided screening strategies have been widely used to identify aroma-enhancing non-Saccharomyces yeasts from fruits and spontaneous fermentations (; Zhang et al., 2018). Based on its superior aroma intensity and balanced sensory profile, strain W-8 was selected for subsequent sequencing and phylogenetic analysis.
3.2 Identification and characterization of a non-canonical yeast isolated from fig
3.2.1 Morphological characterization
Strain W-8 was identified via a polyphasic approach combining morphological and molecular analyses. When cultured on YPD agar at 30 °C for 48–72 h, W-8 formed circular, creamy-white colonies with entire smooth margins and a slightly raised, rough surface (Figure 2A). Light microscopy revealed oval to ellipsoidal vegetative cells, occurring primarily as single cells or small clusters (Figure 2B), while SEM confirmed regular ellipsoidal cells with smooth walls and no hyphal/pseudohyphal structures (Figure 2C). These morphological traits align with the established phenotypic characteristics of the genus Torulaspora (; ; Varela, 2016).
FIGURE 2
3.2.2 Molecular identification based on 26S rDNA sequencing
Molecular identification of strain W-8 was performed by sequencing a 587 bp fragment of the 26S rDNA gene. BLAST analysis showed 100% sequence identity with T. delbrueckii. Consistent with this result, phylogenetic analysis placed strain W-8 firmly within the T. delbrueckii clade (Figure 2D), where it clustered closely with reference T. delbrueckii sequences. Together, these molecular analyses confirm that strain W-8 belongs to Torulaspora delbrueckii (; Serra et al., 2025).
3.2.3 Growth characteristics of the aroma-producing yeast strain W-8
The growth curve of strain W-8 exhibits a lag phase, an exponential growth phase, and a stationary phase at fermentation times of 0–12 h, 12–22 h, and 22 h, respectively (Figure 3). The lag phase likely reflects adaptation of strain W-8 to the fig medium, whereas the stationary phase was likely associated with nutrient depletion and reduced oxygen availability (; ; ; Zhang et al., 2018).
FIGURE 3
3.2.4 Effects of fermentation parameters on aroma production by strain W-8
Figure 4A shows that the production of aroma compounds increased slowly during the first 12 h of fermentation, then increased rapidly to about 95% by the 36th hour; afterward, their production decreased to 58% over the next 48 h. The production of aroma compounds exhibited a trend of increasing initially and then decreasing. This pattern may be associated with the availability or depletion of nutrients and oxygen, as well as microbial growth metabolism at different fermentation stages in the fig medium. Specifically, the yield of aroma compounds increased sharply during the exponential growth phase of the microorganisms. However, as nutrients were heavily consumed and the microbes entered the stationary phase, the compound levels showed a downward trend. Additionally, other factors such as the volatility of aroma compounds and their utilization by microorganisms cannot be ruled out (; Zhang et al., 2018). Figure 4B shows that aroma compound production was about 60% at 25 °C. When the W-8 strain was grown at 30 °C, production increased to 100%. However, further increases in temperature to 32 °C and 35 °C resulted in decreases to 83% and 69%, respectively. These results indicate that 30 °C is the optimal temperature for enzyme activity and cell growth, as yeast generally grows well at 30 °C (Sun et al., 2024). The results shown in Figure 4C indicate that aroma compound production increased as the pH increased from 5 (65%) to 7 (96%); however, at pH 8, production decreased to 78%. The pH of the medium influences cell growth and enzyme activity; therefore, it regulates the production of secondary metabolites in microorganisms (; Thomas et al., 2002).
FIGURE 4
3.2.5 Optimization of fermentation conditions for aroma production by strain W-8
Table 2 summarizes the parameters used in the orthogonal experiments and the corresponding production of aroma compounds by strain W-8. The production of total volatile aroma compounds ranged from 0.556 to 1.359 mg/mL under different fermentation conditions, reflecting the influence of fermentation parameters on aroma formation. The highest production level (1.359 mg/mL) was obtained under condition A2B2C2, corresponding to a fermentation time of 36 h, temperature of 30 °C, and pH 7. These results are consistent with those obtained from the single-factor experiments.
TABLE 2
| Experiment no. | A fermentation time (h) | B fermentation temperature (°C) | C initial pH | Total volatile aroma compounds of W-8 (mg/mL) |
|---|---|---|---|---|
| 1 | 24 | 28 | 6 | 0.55567 |
| 2 | 36 | 28 | 7 | 0.78435 |
| 3 | 48 | 28 | 8 | 0.67751 |
| 4 | 24 | 30 | 8 | 0.92026 |
| 5 | 36 | 30 | 7 | 1.35882 |
| 6 | 48 | 30 | 6 | 1.09489 |
| 7 | 24 | 32 | 7 | 0.92342 |
| 8 | 36 | 32 | 8 | 0.75732 |
| 9 | 48 | 32 | 6 | 0.58806 |
| K1 | 2.40 | 2.02 | 2.24 | |
| K2 | 2.90 | 3.37 | 3.07 | |
| K3 | 2.36 | 2.27 | 2.36 | |
| k1 | 0.80 | 0.67 | 0.75 | |
| k2 | 0.97 | 1.12 | 1.02 | |
| k3 | 0.79 | 0.76 | 0.79 | |
| Range R | 0.18 | 0.45 | 0.28 | |
Orthogonal experiment design and results for optimization of W-8 fermentation process.
Variance analysis further evaluated the relative influence of these parameters (Table 3). The results indicate that fermentation temperature (factor B) significantly affected aroma compound production. The calculated F-value (11.29) was higher than F0.1(2,2) = 9.0 but lower than F0.05(2,2) = 19.0, indicating significance at the α = 0.10 level. In contrast, the variance analysis indicated that pH (C) and fermentation time did not significantly influence the production of aroma compounds.
TABLE 3
| Source of variation | Sum of squares | Degrees of freedom | Mean square | F-value |
|---|---|---|---|---|
| Fermentation time (A) | 0.028 | 2 | 0.014 | 0.82 |
| Fermentation temperature B | 0.385 | 2 | 0.192 | 11.29 |
| Initial pH C | 0.156 | 2 | 0.078 | 4.59 |
| Error | 0.034 | 2 | 0.017 | |
| Total | 0.603 | 8 | | |
Variance analysis of orthogonal experiment results.
F-values were evaluated at α = 0.10 and α = 0.05. indicates significance at α = 0.10, and indicates significance at α = 0.05.
Range (R) analysis further demonstrated that fermentation temperature exerted a greater influence on aroma compound production than pH and fermentation time. Accordingly, the importance of these factors can be ranked as B > C > A. Temperature is known to strongly influence microbial cell growth, membrane fluidity, and enzyme activity; consequently, it plays a critical role in regulating metabolic reactions involved in aroma compound formation (; Papadimitriou et al., 2016; Zhang et al., 2018; Zhang et al., 2024).
Based on the orthogonal experimental results, the optimal fermentation conditions for maximizing aroma compound production were determined to be A2B2C2 (36 h, 30 °C, and pH 7).
3.3 GC–MS analysis of volatile aroma compounds in W-8–fermented fig medium
Table 4 presents the GC–MS results, showing that the total aroma compound production in the fig medium fermented by W-8 increased by 1.64-fold (1.41928 mg/mL) compared with the non-fermented blank control (CK, 0.863 mg/mL).The production of several classes of compounds, including alcohols, esters, ketones, acids, and aldehydes, was altered in the W-8–fermented fig medium.
TABLE 4
| Category | Compound | Rt (min) | Characteristic m/z | RIcalc | RIlit | Match quality (%) | CK (mg/mL) | W-8 (mg/mL) | Change (fold) |
|---|---|---|---|---|---|---|---|---|---|
| Alcohols | Isoamyl alcohol | 9.02 | 45, 55, 70 | 1002 | 998 | 98.73 | — | 0.26398 | Appeared in W-8 |
| p-Menthane-1-ol | 34.04 | 81, 95, 138 | 1356 | 1352 | 85.98 | 0.00172 | 0.00145 | 0.84× (↓) | |
| 2-Methyl-3-pentanol | 72.35 | 56, 69, 87 | 2085 | 2082 | 86.99 | 0.00556 | 0.00618 | 1.11× (↑) | |
| 2-Hexyl-1-decanol | 45.09 | 55, 69, 83 | 1620 | 1617 | 88.54 | 0.00637 | 0.00544 | 0.85× (↓) | |
| (2R,3R)-(−)-2,3-Butanediol | 10.88 | 45, 57, 74 | 1045 | 1043 | 95.61 | — | 0.01390 | Appeared in W-8 | |
| 2,3-Butanediol | 11.34 | 45, 57, 74 | 1058 | 1056 | 91.09 | — | 0.00167 | Appeared in W-8 | |
| Phenethyl alcohol | 32.41 | 91, 108, 122 | 1120 | 1118 | 98.70 | — | 0.05258 | Appeared in W-8 | |
| 2-Butyl-1-octanol | 28.95 | 55, 69, 83 | 1480 | 1478 | 88.46 | 0.00234 | 0.00233 | 1.00× (≈) | |
| Esters | Isoamyl acetate | 8.19 | 43, 60, 70 | 925 | 923 | 95.51 | — | 0.00632 | Appeared in W-8 |
| Lup-20(29)-en-3-yl acetate | 92.83 | 189, 218, 234 | 2580 | 2577 | 89.15 | — | 0.30787 | Appeared in W-8 | |
| Ethyl stearate | 79.46 | 88, 101, 157 | 2210 | 2208 | 89.77 | 0.00652 | 0.02739 | 4.20× (↑) | |
| Ethyl palmitate | 73.35 | 88, 101, 143 | 2105 | 2103 | 92.98 | 0.08531 | 0.07531 | 0.88× (↓) | |
| Ethyl oleate | 78.73 | 55, 69, 83 | 2195 | 2193 | 92.47 | 0.19087 | 0.20359 | 1.07× (↑) | |
| Octadecyl acetate | 79.89 | 43, 55, 69 | 2220 | 2217 | 90.93 | 0.00808 | 0.00870 | 1.08× (↑) | |
| 3-Hydroxy-2-butanone | 8.19 | 43, 45, 88 | 920 | 918 | 95.51 | 0.00926 | 0.02131 | 2.30× (↑) | |
| Ketones | Isophorone | 32.89 | 82, 95, 109 | 1135 | 1133 | 97.42 | 0.03657 | 0.03340 | 0.91× (↓) |
| Isobutyric acid | 10.20 | 43, 45, 73 | 1030 | 1028 | 90.54 | — | 0.01183 | Appeared in W-8 | |
| Acids | Palmitic acid | 72.68 | 60, 73, 129 | 2090 | 2088 | 95.38 | 0.44604 | 0.34481 | 0.77× (↓) |
| 2-Methylbutyric acid | 10.50 | 41, 57, 74 | 1038 | 1036 | 90.00 | — | 0.00411 | Appeared in W-8 | |
| Stearaldehyde | 76.70 | 57, 71, 82 | 2160 | 2158 | 84.79 | 0.04225 | 0.02516 | 0.60× (↓) | |
| Aldehydes | Palmitaldehyde | 70.79 | 57, 71, 82 | 2050 | 2048 | 95.33 | 0.02264 | 0.01553 | 0.69× (↓) |
| 3,4-Dimethylbenzaldehyde | 39.86 | 77, 91, 134 | 1260 | 1258 | 98.62 | 0.01029 | 0.01194 | 1.16× (↑) | |
| Total | Compound | — | — | — | — | — | 0.86316 | 1.41928 | 1.64×(↑) = +64.4% |
Changes of volatile aroma compounds in W-8–fermented Fig Medium.
Strain W-8 produced several key alcohols, among which isoamyl alcohol (0.26398 mg/mL) and phenethyl alcohol (0.05258 mg/mL) were detected exclusively in the fermentation medium. Isoamyl alcohol contributes fruity and alcoholic notes, while phenethyl alcohol contributes a rose-like floral aroma. These compounds belong to microbial volatile organic compounds that are known to influence aroma characteristics in fermented systems (; Mitri et al., 2022; Nancolas et al., 2017; Shemshura et al., 2024). Mechanistically, the exclusive production of these higher alcohols is primarily driven by the Ehrlich pathway, where the yeast metabolizes specific amino acids (such as leucine for isoamyl alcohol and phenylalanine for phenethyl alcohol) through sequential transamination, decarboxylation, and reduction processes. This robust synthesis of volatile alcohols is consistent with findings in fruit fermentations, where T. delbrueckii is frequently reported to elevate fruity and floral ester precursors more efficiently than conventional yeast strains (Zhang et al., 2018).
In addition, acetoin (3-hydroxy-2-butanone), which contributes creamy and buttery sensory notes, increased from 0.00926 mg/mL to 0.02131 mg/mL in the W-8–fermented fig medium (Table 4).
The banana-like ester isoamyl acetate (0.00632 mg/mL) was detected only in the W-8–fermented medium (Table 4), indicating enhanced ester formation during fermentation. Ethyl stearate, ethyl oleate, and octadecyl acetate, which are known to improve mouthfeel, are commonly found in fermented products (; ; Nancolas et al., 2017; Saerens et al., 2010; Verstrepen et al., 2003). Among these compounds, ethyl stearate increased by 4.20-fold (0.02739 mg/mL), whereas ethyl oleate and octadecyl acetate showed moderate increases (Table 4).
The production of isobutyric acid (0.01183 mg/mL) and 2-methylbutyric acid (0.00411 mg/mL) was detected only in the W-8–fermented medium (Table 4). At low concentrations, these compounds can enhance aroma complexity (; ).
In contrast, the production of long-chain fatty acids, such as palmitic acid, decreased from 0.44604 to 0.34481 mg/mL (Table 4), a phenomenon commonly observed in aroma-producing strains (). Similarly, the production of most aldehydes, including stearaldehyde and palmitaldehyde, also decreased. These reductions may be associated with a lower accumulation of compounds related to fatty off-notes (; ; ; Paramithiotis et al., 2025).
Overall, the changes in volatile compound composition are consistent with the enhanced floral, fruity, and sweet sensory characteristics observed in the W-8–fermented fig medium.
3.4 Genomic features and functional metabolic potential of W-8 strain
Whole-genome sequencing of Torulaspora delbrueckii W-8 revealed a genome size of 9224546 bp, consistent with previously reported genome sizes of non-Saccharomyces yeasts (). The genome assembly showed good continuity, with an N50 of 836,209 bp, indicating a reliable assembly suitable for downstream functional analysis (Walker et al., 2014). The GC content was 40.69%. Compared to the model strain CBS1146 (genome size 9.52 Mb, GC content 41.9%), there are some minor differences (); however, it remains comparable to that of other T. delbrueckii strains. Additionally, a total of 4,697 protein-coding genes were predicted (Table 5).
TABLE 5
| Indicator | Total length (bp) | GC content (%) | N50 | Number of CAZy annotated genes | Number of predicted genes |
|---|---|---|---|---|---|
| Results | 9224546 | 40.69 | 836209 | 33 | 4697 |
Basic genomic characteristics of Torulaspora delbrueckii W-8.
KEGG functional annotation assigned 4,050 genes to known biological pathways. As shown in Figure 5, metabolism represented the largest functional category (1,527 genes), reflecting the strong metabolic potential of this strain. Genetic information processing (826 genes), organismal systems (635 genes), and cellular processes (594 genes) were also well represented, suggesting robust transcriptional activity and effective cellular regulation during fermentation. In addition, 468 genes associated with environmental information processing indicate the capacity of strain W-8 to respond to environmental changes during fermentation.
FIGURE 5
Within the metabolism category, carbohydrate metabolism (287 genes) and amino acid metabolism (244 genes) were the most enriched subpathways. These pathways are closely linked to the formation of volatile compounds during fermentation, indicating a strong genetic capacity of the W-8 strain for converting carbohydrates and amino acids into aroma-related metabolites. Specifically, the high abundance of amino acid metabolism genes provides a robust genetic foundation for the aforementioned Ehrlich pathway. This genomic trait directly explains the underlying biological mechanism for the elevated levels of higher alcohols (e.g., isoamyl alcohol and phenethyl alcohol) observed in our GC-MS data. Furthermore, active carbohydrate metabolism ensures a continuous supply of carbon skeletons and energy (ATP) required for the subsequent synthesis of volatile esters.
Genes related to nucleotide metabolism (152 genes), energy metabolism (121 genes), and cofactor and vitamin metabolism (112 genes) further support active cellular growth and enzymatic activity during fermentation ().
Additional genes involved in glycan biosynthesis and metabolism (80 genes) and xenobiotic biodegradation (53 genes) suggest the ability of strain W-8 to adapt to the complex biochemical environment of fig fermentation. A smaller set of genes associated with secondary metabolite biosynthesis (26 genes) and terpenoid and polyketide metabolism (25 genes) also indicates potential for producing diverse bioactive compounds contributing to aroma complexity (; ; Varela, 2016).
The x-axis represents the specific secondary metabolic pathways, while the y-axis indicates the absolute number of genes assigned to each pathway. The pathways are grouped and color-coded into five major functional categories: Cellular Processes (dark green), Environmental Information Processing (blue), Genetic Information Processing (orange), Metabolism (red), and Organismal Systems (light green).
3.4.1 GO functional annotation and its metabolic relevance in W-8 strain
Gene Ontology (GO) annotation of T. delbrueckii W-8 generated 334,317 annotations distributed across three GO domains-biological process, cellular component, and molecular function-covering 48 functional subclasses (Figure 6; Table 6). The biological process category accounted for the largest proportion of annotations, indicating active metabolic and biosynthetic processes in strain W-8 (; Santiago et al., 2021; Silva-Sousa et al., 2024; Tocci et al., 2023). This genetic enrichment provides a theoretical metabolic foundation that supports the diverse volatile metabolite production observed in the GC-MS analysis, which revealed diverse metabolites in the W-8–fermented fig medium (). The cellular component category highlighted numerous membrane-associated proteins involved in nutrient transport, ethanol tolerance, and volatile compound secretion (Ma and Liu, 2010). Although the molecular function category contained fewer annotations, many genes encoding catalytic and binding proteins were identified. These included oxidoreductases, transferases, and hydrolases, suggesting that strain W-8 possesses strong enzymatic potential for biochemical transformations during fermentation (Pinu et al., 2017; Styger et al., 2011).
FIGURE 6
TABLE 6
| Categories | Gene counts |
|---|---|
| Biological process | 224809 |
| Cellular component | 71211 |
| Molecular function | 38297 |
GO annotation across three primary categories.
The annotated genes are classified into three primary GO domains, indicated by distinct colors along the x-axis: biological process (dark green), cellular component (blue), and molecular function (orange). The left y-axis shows the percentage of annotated genes, while the right y-axis indicates the absolute number of genes assigned to each specific functional sub-category.
3.4.2 CAZy database annotation of W-8 strain
CAZy annotation indicated that strain W-8 encodes 33 carbohydrate-active enzyme genes distributed across four CAZy classes: AA (11), CE (3), GH (8), and GT (11) (Table 7). The GH enzyme set includes several activities related to carbohydrate utilization in fig substrates. For example, invertase (EC 3.2.1.26) catalyzes the hydrolysis of sucrose into glucose and fructose, thereby increasing the availability of fermentable sugars during early growth (Manoochehri et al., 2020). Similarly, enzymes such as α-amylase, pullulanase, glucoamylase, α-glucosidase, and α-1,6-glucosidase suggest the capacity of strain W-8 to hydrolyze starch, dextrins, and branched glucans into assimilable sugars (). The presence of endo-inulinase further indicates the potential utilization of fructan-type carbohydrates, reflecting metabolic versatility commonly observed in non-Saccharomyces fermentations ().
TABLE 7
| Enzyme | Family | Gene ID |
|---|---|---|
| α-Amylase (EC 3.2.1.1) | GH | ctg00012_0004316_t |
| Pullulanase (EC 3.2.1.41) | GH | ctg00012_0004316_t |
| Invertase (EC 3.2.1.26) | GH | ctg00016_0004534_t; ctg00003_0001668_t |
| Endo-inulinase (EC 3.2.1.7) | GH | ctg00016_0004534_t; ctg00003_0001668_t |
| Glucan 1,3-β-glucosidase (EC 3.2.1.58) | GH | ctg00007_0003034_t |
| GH | ctg00008_0003567_t | |
| Exo-β-1,4-glucanase (EC 3.2.1.74) | GH | ctg00008_0003567_t |
| Glucan endo-1,6-β-glucosidase (EC 3.2.1.75) | GH | ctg00008_0003567_t |
| Endo-β-1,4-glucanase (EC 3.2.1.4) | GH | ctg00008_0003567_t |
| α-Mannosidase (EC 3.2.1.113) | GH | ctg00001_0000022_t; ctg00001_0000047_t |
| β-Glucosidase (EC 3.2.1.21) | GH | ctg00008_0003567_t |
| Glucoamylase (EC 3.2.1.3) | GH | ctg00004_0001849_t |
| α-Glucosidase (EC 3.2.1.20) | GH | ctg00012_0004316_t |
| Starch α-1,6-glucosidase (EC 3.2.1.33) | GH | ctg00012_0004316_t |
Analysis of CAZy enzyme gene clusters in strain W-8.
A particularly important enzyme identified in this study is β-glucosidase (EC 3.2.1.21) (Table 7). In fruits such as fig, many aroma-related terpenes and phenolic compounds occur in glycosidically bound, non-volatile forms. β-Glucosidase can hydrolyze these bonds and release free volatile aroma compounds (Schober et al., 2023). This observation strongly aligns with previous genomic and transcriptomic studies on wine strains of T. delbrueckii, which highlight its intrinsic β-glucosidase activity as a critical driver for unraveling bound aroma precursors and enhancing the floral complexity of fermented fruit substrates (; Silva-Sousa et al., 2024). In addition, GT enzymes are involved in glycosylation processes that contribute to cellular stability and stress tolerance during fermentation ().
CAZy annotation results also highlight the potential of strain W-8 to release bound aroma precursors and enhance aroma compound formation during fermentation.
4 Conclusion
Natural aroma compounds are increasingly preferred over synthetic ones for several reasons, particularly their perceived health benefits and higher product quality. Keeping this in mind, this study aimed to use a non-Saccharomyces yeast for the production of aroma compounds using figs as the fermentation substrate. T. delbrueckii W-8 was isolated from figs and applied to fig fermentation under optimized culture conditions. As a result, T. delbrueckii W-8 enhanced the production of aroma compounds in fermented figs by 1.64-fold. The strain increased the production of several aroma compounds, including isoamyl alcohol, phenethyl alcohol, isoamyl acetate, isobutyric acid, and 2-methylbutyric acid, as well as elevated levels of ethyl stearate and acetoin during fermentation, indicating the broad substrate conversion potential of this strain. KEGG, GO, and CAZy analyses further revealed that T. delbrueckii W-8 possesses genetic potential for carbohydrate metabolism and enzymatic activities that may contribute to aroma compound formation during fermentation. The findings also suggest that similar bioconversion strategies could be applied to other fruit substrates to improve their flavor complexity and nutritional value. Although the current study successfully demonstrated the potential of the native strain W-8 in enhancing fig aroma, it should be noted that a commercial reference yeast (such as S. cerevisiae) was not included as a positive control. Future studies will incorporate comparative fermentation trials with standard commercial strains to further evaluate the competitive advantages of W-8 in industrial applications.
Statements
Data availability statement
The original contributions presented in the study are publicly available. This data can be found in the NCBI GenBank database under accession number PX915611. The data can be accessed via https://www.ncbi.nlm.nih.gov/nuccore/PX915611.
Author contributions
ZC: Writing – original draft, Writing – review and editing. YS: Investigation, Software, Writing – review and editing. LL: Data curation, Methodology, Writing – review and editing. QnW: Formal Analysis, Supervision, Writing – review and editing. YZ: Formal Analysis, Project administration, Writing – review and editing. WQ: Validation, Writing – review and editing. SH: Funding acquisition, Validation, Writing – review and editing. YN: Funding acquisition, Resources, Writing – review and editing. QuW: Funding acquisition, Resources, Visualization, Writing – review and editing.
Funding
The author(s) declared that financial support was received for this work and/or its publication. This study was sponsored by the Natural Science Foundation of China (22401268), and Henan Zhongyan Industry Co., Ltd. Technology Project (AW2023013). The funders were not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication.
Conflict of interest
Authors ZC, LL, QnW, YZ, YN, and QuW were employed by Technology Center for China Tobacco Henan Industrial Limited Company.
The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Generative AI statement
The author(s) declared that generative AI was not used in the creation of this manuscript.
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References
1
AlbertinW.MarulloP. (2012). Polyploidy in fungi: evolution after whole-genome duplication. Proc. R. Soc. B Biol. Sci.279, 2497–2509. 10.1098/rspb.2012.0434
2
ArshadY.HaqI. U.AftabM. N.NawazA. (2024). Biosynthesis of microbial urease and its optimization for enhanced production. Pak. J. Bot.56 (3), 23. 10.30848/PJB2024-3
3
ArvanitiO. S.SamarasY.GatidouG.ThomaidisN. S.StasinakisA. S. (2019). Review on fresh and dried figs: chemical analysis and occurrence of phytochemical compounds, antioxidant capacity and health effects. Food Res. Int.119, 244–267. 10.1016/j.foodres.2019.01.055
4
AyusoM.CarpenaM.TaofiqO.AlbuquerqueT. G.Simal-GandaraJ.OliveiraM. B. P. P.et al (2022). Fig “Ficus carica L.” and its by-products: a decade evidence of their health-promoting benefits towards the development of novel food formulations. Trends Food Sci. Technol.127, 1–13. 10.1016/j.tifs.2022.06.010
5
BastíasC.PérezY.Kessi-PérezE. I.GuillamónJ. M.MartínezC. (2025). Specific point mutations in the RRT5 gene modulate fermentative phenotypes of an industrial wine yeast. Fermentation11 (2), 88. 10.3390/fermentation11020088
6
BenitoS. (2018). The impact of Torulaspora delbrueckii yeast in winemaking. Appl. Microbiol. Biotechnol.102 (Number 7), 3081–3094. 10.1007/s00253-018-8849-0
7
BisschopsM. M. M.VosT.Martínez-MorenoR.CortésP. de la T.PronkJ. T.Daran-LapujadeP. (2015). Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae. Microb. Cell2 (11), 429–444. 10.15698/mic2015.11.238
8
BizzioL. N.TiemanD.MunozP. R. (2022). Branched-chain volatiles in fruit: a molecular perspective. Front. Plant Sci.12, 814138. 10.3389/fpls.2021.814138
9
BroachJ. R. (2012). Nutritional control of growth and development in yeast. Genetics192 (Number 1), 73–105. 10.1534/genetics.111.135731
10
BuchfinkB.XieC.HusonD. H. (2014). Fast and sensitive protein alignment using DIAMOND. Nat. Methods12 (1), 59–60. 10.1038/nmeth.3176
11
CanonicoL.CianiE.GalliE.ComitiniF.CianiM. (2020). Evolution of aromatic profile of Torulaspora delbrueckii mixed fermentation at microbrewery plant. Fermentation6 (1), 7. 10.3390/fermentation6010007
12
CantarelB. I.CoutinhoP. M.RancurelC.BernardT.LombardV.HenrissatB. (2009). The carbohydrate-active EnZymes database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res.37 (Suppl. 1), D233–D238. 10.1093/nar/gkn663
13
CaspetaL.CastilloT.NielsenJ. (2015). Modifying yeast tolerance to inhibitory conditions of ethanol production processes. Front. Bioeng. Biotechnol.3, 184. 10.3389/fbioe.2015.00184
14
CatrileoD.Acuña-FontecillaA.GodoyL. (2020). Adaptive laboratory evolution of native Torulaspora delbrueckii YCPUC10 with enhanced ethanol resistance and evaluation in Co-inoculated fermentation. Front. Microbiol.11, 595023. 10.3389/fmicb.2020.595023
15
ChebotarI. V.NovikovI. A.SubbotA. M.MayanskyN. A. (2017). Lanthanoid staining as a fast technology of preparing microbiological specimens for scanning electron microscopy. Sovrem. Tehnol. V. Med.9 (3), 23. 10.17691/stm2017.9.3.03
16
ChengY.GengS.ZhangJ.ZhaoX.JiangJ.LiangY.et al (2025). A comprehensive study on fermentation and aroma contributions of Torulaspora delbrueckii in diverse wine varieties: insights from pure and co-fermentation studies. Food Res. Int.199, 115340. 10.1016/j.foodres.2024.115340
17
CianiM.CapeceA.ComitiniF.CanonicoL.SiestoG.RomanoP. (2016). Yeast interactions in inoculated wine fermentation. Front. Microbiol.7 (Number APR), 555. 10.3389/fmicb.2016.00555
18
de OvalleS.BrenaB.González-PomboP. (2021). Influence of beta glucosidases from native yeast on the aroma of Muscat and tannat wines. Food Chem.346, 128899. 10.1016/j.foodchem.2020.128899
19
Delač SalopekD.HorvatI.HranilovićA.PlavšaT.RadekaS.PaskovićI.et al (2022). Diversity of volatile aroma compound composition produced by non-saccharomyces yeasts in the early phase of grape must fermentation. Foods11 (19), 3088. 10.3390/foods11193088
20
Díaz-MarotoM. C.SchneiderR.BaumesR. (2005). Formation pathways of ethyl esters of branched short-chain fatty acids during wine aging. J. Agric. Food Chem.53 (9), 3503–3509. 10.1021/jf048157o
21
Díaz-MontesE.Castro-MuñozR. (2021). Edible films and coatings as food-quality preservers: an overview. Foods10 (Number 2), 249. 10.3390/foods10020249
22
DzialoM. C.ParkR.SteenselsJ.LievensB.VerstrepenK. J. (2017). Physiology, ecology and industrial applications of aroma formation in yeast. FEMS Microbiol. Rev.41, S95–S128. 10.1093/femsre/fux031
23
EddyS. R. (2011). Accelerated profile HMM searches. PLoS Comput. Biol.7 (10), e1002195. 10.1371/journal.pcbi.1002195
24
FarB. E.AhmadiY.KhosroushahiA. Y.DilmaghaniA. (2020). Microbial alpha-amylase production: progress, challenges and perspectives. Adv. Pharm. Bull.10 (Number 3), 350–358. 10.34172/apb.2020.043
25
FernandesT.Silva-SousaF.PereiraF.RitoT.SoaresP.Franco-DuarteR.et al (2021). Biotechnological importance of torulaspora delbrueckii: from the obscurity to the spotlight. J. Fungi7 (9), 712. 10.3390/jof7090712
26
FukudaK.YamamotoN.KiyokawaY.YanagiuchiT.WakaiY.KitamotoK.et al (1998). Balance of activities of alcohol acetyltransferase and esterase in Saccharomyces cerevisiae is important for production of isoamyl acetate. Appl. Environ. Microbiol.64 (10), 4076–4078. 10.1128/aem.64.10.4076-4078.1998
27
HazelwoodL. A.DaranJ. M.Van MarisA. J. A.PronkJ. T.DickinsonJ. R. (2008). The Ehrlich pathway for fusel alcohol production: a century of research on Saccharomyces cerevisiae metabolism. Appl. Environ. Microbiol.74 (Number 8), 2259–2266. 10.1128/AEM.02625-07
28
HenriquesD.MineboisR.MendozaS. N.MacíasL. G.Pérez-TorradoR.BarrioE.et al (2021). A multiphase multiobjective dynamic genome-scale model shows different redox balancing among yeast species of the saccharomyces genus in fermentation. MSystems6 (4), e0026021. 10.1128/msystems.00260-21
29
HoltS.MiksM. H.De CarvalhoB. T.Foulquié-MorenoM. R.TheveleinJ. M. (2019). The molecular biology of fruity and floral aromas in beer and other alcoholic beverages. FEMS Microbiol. Rev.43 (Number 3), 193–222. 10.1093/femsre/fuy041
30
JanuszekM.SatoraP.WajdaL.TarkoT. (2020). Saccharomyces bayanus enhances volatile profile of apple brandies. Molecules25 (14), 3127. 10.3390/molecules25143127
31
KanehisaM.GotoS. (2000). KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res.2827–30. 10.1093/nar/28.1.27
32
KimS. H.WitteC. P.RheeS. (2021). Structural basis for the substrate specificity and catalytic features of pseudouridine kinase from Arabidopsis thaliana. Nucleic Acids Res.49 (1). 10.1093/nar/gkaa1144
33
LiuL.ZhaoP. T.HuC. Y.TianD.DengH.MengY. H. (2022). Screening low-methanol and high-aroma produced yeasts for cider fermentation by transcriptive characterization. Front. Microbiol.13, 1042613. 10.3389/fmicb.2022.1042613
34
LombardV.Golaconda RamuluH.DrulaE.CoutinhoP. M.HenrissatB. (2014). The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res.42 (D1), D490–D495. 10.1093/nar/gkt1178
35
LomsadzeA.BurnsP. D.BorodovskyM. (2014). Integration of mapped RNA-Seq reads into automatic training of eukaryotic gene finding algorithm. Nucleic Acids Res.42 (15), e119. 10.1093/nar/gku557
36
MaM.LiuZ. L. (2010). Mechanisms of ethanol tolerance in saccharomyces cerevisiae. Appl. Microbiol. Biotechnol.87 (Number 3), 829–845. 10.1007/s00253-010-2594-3
37
ManoochehriH.HosseiniN. F.SaidijamM.TaheriM.RezaeeH.NouriF. (2020). A review on invertase: its potentials and applications. Biocatal. Agric. Biotechnol.25, 101599. 10.1016/j.bcab.2020.101599
38
MeliniF.MeliniV. (2024). Role of microbial fermentation in the bio-production of food aroma compounds from vegetable waste. Fermentation10 (3), 132. 10.3390/fermentation10030132
39
MitriS.KoubaaM.MarounR. G.RossignolT.NicaudJ. M.LoukaN. (2022). Bioproduction of 2-Phenylethanol through yeast fermentation on synthetic media and on agro-industrial waste and By-Products: a review. Foods11 (Number 1), 109. 10.3390/foods11010109
40
NancolasB.BullI. D.StennerR.DufourV.CurnowP. (2017). Saccharomyces cerevisiae Atf1p is an alcohol acetyltransferase and a thioesterase in vitro. Yeast34 (6), 239–251. 10.1002/yea.3229
41
OliveiraA. P.SilvaL. R.Guedes de PinhoP.Gil-IzquierdoA.ValentãoP.SilvaB. M.et al (2010). Volatile profiling of ficus carica varieties by HS-SPME and GC-IT-MS. Food Chem.123 (2), 548–557. 10.1016/j.foodchem.2010.04.064
42
PadillaB.GilJ. V.ManzanaresP. (2016). Past and future of non-Saccharomyces yeasts: from spoilage microorganisms to biotechnological tools for improving wine aroma complexity. Front. Microbiol.7 (Number MAR), 411. 10.3389/fmicb.2016.00411
43
PapadimitriouK.AlegríaÁ.BronP. A.de AngelisM.GobbettiM.KleerebezemM.et al (2016). Stress Physiology of Lactic Acid Bacteria. Microbiol. Mol. Biol. Rev80 (3), 837–890. 10.1128/MMBR.00076-15
44
ParamithiotisS.PatraJ. K.KotseridisY.DimopoulouM. (2025). Utilization of non-saccharomyces to address contemporary winemaking challenges: species characteristics and strain diversity. Fermentation11 (12), 665. 10.3390/fermentation11120665
45
PinuF. R.Villas-BoasS. G.AggioR. (2017). Analysis of intracellular metabolites from microorganisms: quenching and extraction protocols. Metabolites7 (4), 53. 10.3390/metabo7040053
46
SaerensS. M. G.DelvauxF. R.VerstrepenK. J.TheveleinJ. M. (2010). Production and biological function of volatile esters in Saccharomyces cerevisiae. Microb. Biotechnol.3 (2), 165–177. 10.1111/j.1751-7915.2009.00106.x
47
SantiagoC.RitoT.VieiraD.FernandesT.PaisC.SousaM. J.et al (2021). Improvement of torulaspora delbrueckii genome annotation: towards the exploitation of genomic features of a biotechnologically relevant yeast. J. Fungi7 (4), 287. 10.3390/jof7040287
48
SchoberD.WackerM.SchmarrH. G.FischerU. (2023). Understanding the contribution of Co-Fermenting non-saccharomyces and saccharomyces yeasts to Aroma precursor degradation and formation of sensory profiles in wine using a model system. Fermentation9 (11), 931. 10.3390/fermentation9110931
49
SerraJ. L.NojosaA. da S.CarvalhoA. S. S.RochaL. M. N.PereiraA. L.BastosF. C.et al (2025). Enhancing mead aroma using non-saccharomyces yeast β-Glucosidase producers isolated from honey: a case study in the upper Turi region. Fermentation11 (5), 282. 10.3390/fermentation11050282
50
ShemshuraO.ShemsheyevaZ.AlimzhanovaM.SadanovA.BozenaL.KhalimaK. (2024). Antifungal activity of trichoderma asperеllum and a profile of its volatile organic compounds. Pak. J. Bot.56 (3), 9.
51
Silva-SousaF.FernandesT.PereiraF.RodriguesD.RitoT.CamarasaC.et al (2022). Torulaspora delbrueckii phenotypic and metabolic profiling towards its biotechnological exploitation. J. Fungi8 (6), 569. 10.3390/jof8060569
52
Silva-SousaF.OliveiraB.Franco-DuarteR.CamarasaC.João SousaM. (2024). Bridging the gap: linking Torulaspora delbrueckii genotypes to fermentation phenotypes and wine aroma. FEMS Yeast Res.24, foae034. 10.1093/femsyr/foae034
53
StygerG.PriorB.BauerF. F. (2011). Wine flavor and aroma. J. Industrial Microbiol. Biotechnol.38 (Number 9), 1145–1159. 10.1007/s10295-011-1018-4
54
SunW.ChenX.BiP.HanJ.LiS.LiuX.et al (2024). Screening and characterization of indigenous non-Saccharomyces cerevisiae with high enzyme activity for kiwifruit wine production. Food Chem.440, 138309. 10.1016/j.foodchem.2023.138309
55
Ter-HovhannisyanV.LomsadzeA.ChernoffY. O.BorodovskyM. (2008). Gene prediction in novel fungal genomes using an ab initio algorithm with unsupervised training. Genome Res.18 (12), 1979–1990. 10.1101/gr.081612.108
56
The Gene Ontology Consortium. (2015). Gene ontology consortium: going forward. Nucleic Acids Res.43 (Database issue), D1049–D1056. 10.1093/nar/gku1179
57
ThomasK. C.HynesS. H.IngledewW. M. (2002). Influence of medium buffering capacity on inhibition of Saccharomyces cerevisiae growth by acetic and lactic acids. Appl. Environ. Microbiol.68 (4), 1616–1623. 10.1128/AEM.68.4.1616-1623.2002
58
TocciN.EggerM.HoellriglP.SanollC.BeisertB.BrezinaS.et al (2023). Torulaspora delbrueckii strain behaviour within different refermentation strategies for sparkling cider production. Appl. Sci. Switz.13 (6), 4015. 10.3390/app13064015
59
VarelaC. (2016). The impact of non-Saccharomyces yeasts in the production of alcoholic beverages. Appl. Microbiol. Biotechnol.100 (23), 9861–9874. 10.1007/s00253-016-7941-6
60
VerstrepenK. J.DerdelinckxG.DufourJ. P.WinderickxJ.TheveleinJ. M.PretoriusI. S.et al (2003). Flavor-active esters: adding fruitiness to beer. J. Biosci. Bioeng.96 (Number 2), 110–118. 10.1016/S1389-1723(03)90112-5
61
WalkerB. J.AbeelT.SheaT.PriestM.AbouellielA.SakthikumarS.et al (2014). Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS ONE9 (11), e112963. 10.1371/journal.pone.0112963
62
WangC.LiuL.GuoJ.JikeX.XuK.LiB.et al (2023). Phenolic profiles, antioxidant capacities and flavour volatiles in fig (Ficus carica L.) juices from five cultivars fermented by Lactobacillus plantarum and Lactobacillus acidophilus. Int. J. Food Sci. Technol.58 (11), 6025–6035. 10.1111/ijfs.16711
63
WatsonL. P.McKeeA. E.MerrellB. R. (1980). Preparation of microbiological specimens for scanning electron microscopy. Scanning Electron Microsc. 541–550.
64
YanS.XiangsongC.XiangX. (2019). Improvement of the aroma of lily rice wine by using aroma-producing yeast strain Wickerhamomyces anomalus HN006. Amb. Express9 (1), 89. 10.1186/s13568-019-0811-8
65
ZhangB. Q.LuanY.DuanC. Q.YanG. L. (2018). Use of Torulaspora delbrueckii Co-fermentation with two Saccharomyces cerevisiae strains with different aromatic characteristic to improve the diversity of red wine aroma profile. Front. Microbiol.9 (APR), 606. 10.3389/fmicb.2018.00606
66
ZhangD.MaX.SunX.WangY.ZhaoX.GongR.et al (2024). Optimize the culture and fermentation condition of biocontrol strain ph-120 against sclerotinia sclerotiorum. Pak. J. Bot.56 (5), 10. 10.30848/PJB2024-5
Summary
Keywords
aroma compounds, fermentation, FIG, T.delbrueckii, whole-genome sequencing
Citation
Chen Z, Shentu Y, Li L, Wang Q, Zhao Y, Qi W, Huang S, Ning Y and Wang Q (2026) Screening of flavor-enhancing yeast and its role in fig fermentation. Front. Bioeng. Biotechnol. 14:1807323. doi: 10.3389/fbioe.2026.1807323
Received
09 February 2026
Revised
15 April 2026
Accepted
20 April 2026
Published
12 May 2026
Volume
14 - 2026
Edited by
Huadong Peng, University of Queensland, Australia
Reviewed by
Pranjit Kumar Bora, Indian Institute of Technology Guwahati, India
Flávia Silva-Sousa, University of Minho, Portugal
Updates
Copyright
© 2026 Chen, Shentu, Li, Wang, Zhao, Qi, Huang, Ning and Wang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Yibo Ning, hnzynyb@163.com; Qiuling Wang, jrwql395139@sina.com
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.