AUTHOR=Huang Ziru , Gunarathne Samarappuli Mudiyanselage Savini , Liu Wenwen , Zhou Yuwei , Jiang Yuqing , Li Shiqi , Huang Jian 

TITLE=PhIP-Seq: methods, applications and challenges

JOURNAL=Frontiers in Bioinformatics

VOLUME=Volume 4 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/bioinformatics/articles/10.3389/fbinf.2024.1424202

DOI=10.3389/fbinf.2024.1424202

ISSN=2673-7647

ABSTRACT=Phage-immunoprecipitation sequencing (PhIP-Seq) technology is an innovative, high-throughput antibody detection method. It enables comprehensive analysis of individual antibody profiles. This technology shows great potential, particularly in exploring disease mechanisms and immune responses. Currently, PhIP-Seq has been successfully applied in various fields, such as the exploration of biomarkers for autoimmune diseases, vaccine development, and allergen detection. A variety of bioinformatics tools have facilitated the development of this process. However, PhIP-Seq technology still faces many challenges and has room for improvement. Here, we review the methods, applications, and challenges of PhIP-Seq and discuss its future directions in immunological research and clinical applications. With continuous progress and optimization, PhIP-Seq is expected to play an even more important role in future biomedical research, providing new ideas and methods for disease prevention, diagnosis, and treatment.