AUTHOR=Wang Shuang , Cao Yun-Feng , Sun Xiao-Yu , Hong Mo , Fang Zhong-Ze , Luo Hui-Huan , Sun Huan , Yang Ping 

TITLE=Plasma Amino Acids and Residual Hypertriglyceridemia in Diabetic Patients Under Statins: Two Independent Cross-Sectional Hospital-Based Cohorts

JOURNAL=Frontiers in Cardiovascular Medicine

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2021.605716

DOI=10.3389/fcvm.2021.605716

ISSN=2297-055X

ABSTRACT=Abstract 
Objective To investigate the relationship of amino acids metabolism with effect of statins on hypertriglyceridemia in diabetic patients free of prior cardiovascular diseases. 
Methods Two independent cross-sectional hospital based cohorts, i.e., Liaoning Medical University First Affiliated Hospital (LMUFAH, n=146) and the Second Affiliated Hospital of Dalian Medical University (SAHDMU, n=294) were included in current analysis. Hypertriglyceridemia was defined as triglyceride ≥ 1.7 mmol/L;  well-controlled LDL-C was defined as <2.6 mmol/L. The adjusted ORs (95% CI) of circulating metabolic measures for hypertriglyceridemia were assessed using logistic regression. Pooled results of metabolites with same direction of association in both cohorts were combined using inverse variance-weighted fixed-effect meta-analysis. Difference of identified metabolites in patients with and without hypertriglyceridemia were also obtained in the context of LDL-C.
Results 86 and 106 patients were with hypertriglyceridemia in LMUFAH and SAHDMU, respectively. We observed that elevated alanine, asparagine, leucine and valine were consistently associated with increased hypertriglyceridemia in both cohorts. In fixed effect pooled analysis, the OR (95%CI) of per SD increased was 1.71 (1.32-2.20)  for alanine, 1.62 (1.20-2.19)  for asparagine, 1.64 (1.22-2.20)  for leucine and 1.62 (1.22-2.13) for valine (all P value ranged from 0.0018 to <.0001); adjusting for C-peptide attenuated effect sizes of Ala, Leu and Val for hypertriglyceridemia. The difference were robust in groups with well- or bad-controlled LDL-C. 
Conclusion Alanine, asparagine, leucine and valine were positively associated with increased residual risk of hypertriglyceridemia in diabetic patients with statins treatment.