AUTHOR=Wang Xueyu , Fu Yahong , Xie Zulong , Cao Muhua , Qu Wenbo , Xi Xiangwen , Zhong Shan , Piao Minghui , Peng Xiang , Jia Ying , Meng Lingbo , Tian Jinwei TITLE=Establishment of a Novel Mouse Model for Atherosclerotic Vulnerable Plaque JOURNAL=Frontiers in Cardiovascular Medicine VOLUME=Volume 8 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2021.642751 DOI=10.3389/fcvm.2021.642751 ISSN=2297-055X ABSTRACT=Background and aims: Acute coronary syndrome (ACS) is a group of clinical syndromes characterized by rupture or erosion of atherosclerotic unstable plaques. Effective intervention for vulnerable plaques (VP) is of great significance to reduce adverse cardiovascular events. Methods: Fbn1C1039G+/- mice were crossbred with LDLR−/− mice to obtain a novel model for atherosclerotic VP. After fed with a high-fat diet (HFD) for 12 or 24 weeks, several different pathological staining and immunohistochemistry were employed to evaluate the atherosclerotic lesion. Results: Compared to control mice, Fbn1C1039G+/-LDLR−/− mice developed more severe atherosclerotic lesions, the positive area of Oil Red O staining in aortic sinus were significantly increased after 12 weeks (21.7±2.0 vs 6.3±2.1) and 24 weeks (32.6±2.5 vs 18.7±2.6), respectively. More vulnerable plaque characteristics, significantly larger necrotic core (280±19 vs 105±7), thinner fiber cap (14.0±2.8 vs 32.6±2.7), apparent elastin fibers fragmentation and vessel dilation (3010±67 vs 1465±49), 2-fold augment of macrophages (8.5±1.0 vs 5.0±0.6),obviously decreased smooth muscle cell (0.6±0.1 vs 2.1±0.2)and about 25% decrease in total collagen (33.6±0.3 vs 44.9±9.1) were observed in Fbn1C1039G+/-LDLR−/− compared to the control after 24 weeks. Furthermore, spontaneous plaque rupture, neovascularization, and intraplaque hemorrhage were detected in the model mice plaque regions instead of the control mice. Conclusions: Plaques in Fbn1C1039G+/-LDLR−/− mice fed on HFD show many features of human advanced atherosclerotic unstable plaques. These results suggest that Fbn1C1039G+/-LDLR−/− is a novel model for investigating the pathological and physiological mechanisms of advanced atherosclerotic unstable plaques.