Role of the Mitochondrial Protein Import Machinery and Protein Processing in Heart Disease

Abstract

Mitochondria are essential organelles for cellular energy production, metabolic homeostasis, calcium homeostasis, cell proliferation, and apoptosis. About 99% of mammalian mitochondrial proteins are encoded by the nuclear genome, synthesized as precursors in the cytosol, and imported into mitochondria by mitochondrial protein import machinery. Mitochondrial protein import systems function not only as independent units for protein translocation, but also are deeply integrated into a functional network of mitochondrial bioenergetics, protein quality control, mitochondrial dynamics and morphology, and interaction with other organelles. Mitochondrial protein import deficiency is linked to various diseases, including cardiovascular disease. In this review, we describe an emerging class of protein or genetic variations of components of the mitochondrial import machinery involved in heart disease. The major protein import pathways, including the presequence pathway (TIM23 pathway), the carrier pathway (TIM22 pathway), and the mitochondrial intermembrane space import and assembly machinery, related translocases, proteinases, and chaperones, are discussed here. This review highlights the importance of mitochondrial import machinery in heart disease, which deserves considerable attention, and further studies are urgently needed. Ultimately, this knowledge may be critical for the development of therapeutic strategies in heart disease.

Introduction

Mitochondria are vital for energy production in eukaryotic cells, generating cellular ATP through oxidative phosphorylation (OXPHOS) (1). Importantly, mitochondria are also crucial for numerous metabolic pathways, maintenance of calcium homeostasis, and regulation of cell proliferation and apoptosis (2). However, only 13 proteins involved in OXPHOS are encoded by the mitochondrial genome in mammals. About 99%—more than 1,500—mammalian mitochondrial proteins are encoded by the nuclear genome, synthesized as precursors in the cytosol, and need to be imported into mitochondria by mitochondrial protein import machinery (3). To date, six translocases of the mitochondrial protein import machinery have been discovered. The TOM complex serves as the entry gate for most precursors at the outer membrane (OM); the TIM22 and TIM23 complexes at the inner membrane (IM) are responsible for the insertion of carrier precursors into the IM and the translocation of presequence-carrying precursors into the mitochondrial matrix or IM individually; the mitochondrial intermembrane space (IMS) import and assembly machinery (MIA) complex mediates the import of cysteine-rich proteins to the IMS; the SAM and MIM complexes are responsible for insertion of β-barrel proteins and α-helical proteins, respectively, into the OM (Figure 1 and Table 1) (3). The dynamic interaction and cooperation of these mitochondrial protein import pathways enable cells to respond to environmental stress and energy demands rapidly and with plasticity. Further, mitochondrial protein import pathways function not only as independent units for protein translocation, but also are deeply integrated into a functional network of mitochondrial bioenergetics, protein quality control, mitochondrial dynamics and morphology, and interaction with other organelles (4). Mitochondrial protein import deficiency is linked to various diseases, including neuropathies, myopathies, neurodegenerative diseases, cancer, and cardiovascular disorders (5, 6). The heart is a high-energy–requiring organ that depends heavily on mitochondrial activity and the efficient import of mitochondrial proteins. However, in heart disease, the roles of the mitochondrial protein import machinery have not been well-studied. Here, we summarize the current knowledge on mitochondrial protein import in heart disease for the first time. This review highlights the importance of the mitochondrial import machinery in heart disease, which deserves considerable attention, and further studies are urgently needed. Ultimately, this knowledge may be critical for the development of therapeutic strategies in heart disease.

Figure 1

Overview of Mitochondrial Protein Import Machinery (In Yeast)

Presequence Pathway to the Mitochondrial Matrix and IM

The presequence pathway is the best characterized pathway, responsible for the import of ~60% of all mitochondrial proteins (7). The precursor proteins in this pathway carry a cleavable N-terminal presequence that functions as a targeting signal (7–9). This unique feature of the pathway distinguishes it from all the others, where the precursor proteins do not have cleavable presequences, but possess different kinds of internal targeting signals. Presequence-carrying precursors to the mitochondrial matrix are typically recognized by the translocase of the outer membrane (TOM) (9–11), passaged through the IM by the translocase of the inner membrane (TIM23) (12–16), and driven into the matrix assisted by the presequence translocase-associated motor (PAM) (Figure 1) (17–24). The membrane potential (Δψ) across the IM is essential for the activation of the TIM23 channel and the translocation of presequences into the matrix (25–28). The presequences are cleaved by the mitochondrial processing peptidase (MPP) (3, 29–31), and additional proteolytic processing occurs by intermediate cleaving peptidases (7, 32–34). Presequence-carrying precursors that integrate into the IM follow two distinct routes (Figure 1). IM proteins are either directly released laterally from the TIM23 complex (35–37) or transported first into the matrix, followed by further insertion into the IM with the help of the oxidase assembly protein 1 (Oxa1) insertase (38–41). Oxa1 also is responsible for insertion of IM proteins synthesized on mitochondrial ribosomes (42).

The presequences are located at the N-termini of preproteins and typically consist of ~15–50 amino acids. An essential characteristic of mitochondrial presequences is the formation of an amphipathic α-helix that is specifically recognized by mitochondrial import receptors and other mitochondrial import components during preprotein translocation by TOM, TIM23, and PAM (3).

The TOM complex is the main gate for precursors entering mitochondria. The TOM complex is composed of Tom20, Tom22, and Tom70 as receptors, β-barrel protein Tom40 forming the channel, and three small associated proteins, Tom5, Tom6, and Tom7 (11, 43–45). Tom20 and Tom22 function cooperatively as the receptors for presequence-carrying precursors (9, 10, 46). Tom70 mainly functions as the receptor for preproteins with internal targeting sequences, such as carrier precursors (47–52). Presequence-carrying precursors cross the Tom40 channel as linear polypeptide chains (44, 45, 53–57), and interact with the tail of the Tom22 receptor in the IMS (57). Tom22 has presequence binding sites on both the cytoplasmic and IMS sides of the OM. The exact roles of the three small subunits, Tom5, Tom6, and Tom7, have not been well-clarified. It has been proposed that they are not essential for TOM functions but are involved in the assembly and stability of the TOM complex (58–61).

The TIM23 complex translocates cleavable preproteins into mitochondrial matrix or IM. Tim50, Tim23, Tim17, and Tim21 compose the main elements of the TIM23 complex (12, 13, 16, 62–65). Tim50 functions as a presequence receptor that binds preproteins emerging in the IMS (63) and a channel blocker that closes out the TIM23 channel in the absence of preproteins (25, 66–69). Tim23 and associated partner Tim17 form the channel (16, 25, 28, 64, 70, 71). Tim21, Tim50, and Tim23 expose domains to the IMS that transiently connect the TOM and TIM23 complexes to facilitate the preprotein transfer (16, 67, 72–74). Additionally, Tim21 also physically links the TIM23 complex to the respiratory chain III-IV supercomplex [bc1 complex and cytochrome c oxidase (COX)] (65, 75, 76). Tim21 thus plays a dual role in TOM-TIM23 transfer and the recruitment of respiratory chain complexes. A small membrane protein, Mgr2, functions as a lateral gatekeeper for preproteins that are sorted into the IM (35, 76). The Δψ across the IM is crucial for translocation of the presequences through the Tim23 channel, which is negative at the matrix side and positive at the IMS side of the IM, whereas presequences are mostly positively charged. Two roles have been assigned to Δψ: activation of TIM23 channel (25) and an electrophoretic effect that drives the import of presequences (77, 78).

PAM. The Δψ is a prerequisite for translocation of the presequence across the TIM23 channel. Nevertheless, it is not sufficient to import the entire protein into the matrix. PAM is necessary for the translocation of matrix proteins. The core of PAM is formed by the molecular chaperone mitochondrial 70 kDa heat shock protein (mtHsp70) (17, 18) and its co-chaperones (Tim44, Mge1, Pam16, Pam17, and Pam18) (16, 21, 79, 80). mtHsp70 binds the unfolded polypeptide chain and drives its translocation into the matrix in an ATP-dependent manner (17, 18). The peripheral membrane protein Tim44 is a docking site for mtHsp70 at the TIM23 complex (21). Mge1 (also known as mitochondrial GrpE) stimulates the release of ADP from mtHsp70 (81). Pam16, Pam17, and Pam18 are three membrane-associated co-chaperones. Pam18 (also termed Tim14) is a J-type co-chaperone that stimulates the ATPase activity of mtHsp70 (82, 83). The J-related Pam16 (Tim16) forms a complex with Pam18 and functions as a negative regulator (82–86). Pam17 mediates the organization of the TIM23–PAM interaction (79, 87).

MPP. Once arriving in the matrix, the presequences of both IM-sorted and matrix-targeted precursors are removed by a heterodimeric enzyme, MPP (3, 29–31, 88). Additional proteases, the intermediate cleaving peptidase (Icp55) (7, 89) and the octapeptidyl aminopeptidase (Oct1, also termed mitochondrial intermediate peptidase, MIP) (90, 91), can remove destabilizing N-terminal amino acid residues of the imported proteins. mtHSP70 and other chaperones, like the HSP60–HSP10 chaperonin complex, further assist proteins folding into their active forms (92). The clipped presequence peptides undergo subsequent degradation by the matrix peptidasome, termed presequence protease (PreP) or Cym1 (93, 94).

OXA translocase is vital for exporting proteins from the mitochondrial matrix into the IM. OXA has three different roles. (1) Proteins encoded by the mitochondrial genome are exported into the IM by Oxa1 (42, 95–97). (2) Some presequence-carrying proteins imported into the matrix via the TOM-TIM23 machinery are exported into the IM via Oxa1. This import-export pathway is termed conservative sorting of nuclear-encoded IM proteins (38–41, 98–100). (3) Oxa1 is also vital for the assembly of the carrier translocase TIM22 (38, 101).

Carrier Pathway Into the IM

The carrier pathway is the second mitochondrial protein import pathway to be discovered, and is responsible for importing precursor proteins without a cleavable presequence, yet with different kinds of internal targeting signals (3, 102–104). The carrier precursors are accompanied by cytosolic chaperones, such as the HSP70 and HSP90 classes in the cytosol, directly delivered to the Tom70 receptor of the TOM complex (47, 105, 106), and then bound by small TIM chaperones in the IMS (107–110) and eventually integrated into the IM by the carrier translocase of the IM (TIM22) complex in a Δψ-dependent manner (Figure 1) (109, 111–116).

Chaperone-guided transport of carrier precursors (including chaperones in the cytosol and IMS). The carrier import pathway uses the same mitochondrial entry gate as the presequence pathway, the TOM complex. However, the mechanisms of translocation differ significantly. The involvement of cytosolic (47, 105, 106) and mitochondrial IMS chaperones, which is crucial to prevent aggregation of the hydrophobic carrier precursors in the aqueous environment, is the main feature distinguishing this from the presequence pathway. Chaperones of the Hsp70 and Hsp90 classes directly participate in delivering the precursors to Tom70 (47, 105, 106). The receptor Tom70 possesses two distinct binding sites, one for the precursor and another for a chaperone (47, 49, 117), ATP is needed to release the precursor proteins from the cytosolic chaperones (47, 48). Upon binding to Tom70, the carrier precursors are transferred to the central receptor Tom22, followed by insertion into the Tom40 channel in a loop conformation (118, 119), and transferred to small TIM chaperones in the IMS (107–110). These small TIM heterohexameric chaperone complexes, like the Tim9-Tim10 complex (120, 121) and the homologous Tim8-Tim13 complex (122), bind to the precursor proteins and transfer them through the aqueous IMS to IM.

Insertion of carrier precursors into the IM. The TIM22 complex consists of the receptor-like protein Tim54, the channel-forming protein Tim22, the Tim9-Tim10-Tim12 chaperone complex, and the Tim18-Sdh3 module. The majority of Tim54 domain is exposed to the IMS and probably functions as the binding site for the Tim9-Tim10-Tim12 complex (123, 124). The Tim9-Tim10-Tim12 complex is a modified form of the IMS chaperone, docking onto the TIM22 complex (123, 125). Carrier precursors are inserted into the Tim22 channel in a Δψ-dependent manner (115). The Tim18-Sdh3 module is involved in the assembly of the TIM22 complex (126, 127). The carrier precursors are first bound to the Tim9–Tim10–Tim12 chaperone complex on the surface of the translocase. Upon activation of the Tim22 channel (Δψ-dependent), the precursors are inserted into the translocase, probably in a loop structure. Finally, the proteins are laterally released into the lipid phase of the IM.

MIA Complex

Many IMS proteins contain internal targeting signals and characteristic cysteine motifs. In the cytosol, the precursors are kept in a reduced and unfolded state (128). Upon import by the TOM complex (60), they are oxidized by the MIA machinery, and stay in the IMS in an oxidized state (Figure 1). The MIA system consists of two main components: the oxidoreductase Mia40 and the sulfhydryl oxidase Erv1 (129–133).

Mia40 serves as a receptor and protein disulfide carrier. Most IMS proteins are synthesized without cleavable presequences but contain cysteine motifs. Unlike presequence-carrying precursors and carrier precursors, none of the Tom receptors is necessary for the import of MIA substrates (60, 61). Instead, upon passage through the Tom40 channel (60), Mia40 functions as a receptor on the IMS side of the Tom40 channel (133–138). It recognizes an internal signal of the precursor proteins, typically consisting of a hydrophobic element flanked by a cysteine residue (133, 139, 140). Mia40 binds to precursors via hydrophobic interaction and catalyzes the formation of disulfide bonds in imported proteins (133, 138). The disulfide bonds facilitate the conformational stabilization and assembly of many IMS proteins.

Erv1 cooperates with Mia40 in a disulfide relay. Mia40 does not form disulfide bonds de novo. Disulfide bonds are generated by Erv1 and transferred to Mia40 by the formation of transient intermolecular disulfide bonds (141). Mia40 then transfers the disulfides onto the imported protein. Upon transfer of disulfide bonds to proteins, cysteines of Mia40 become reduced and are re-oxidized by Erv1. Electrons originating from the oxidation of imported proteins flow in the opposite direction. They flow from Mia40 to Erv1 and then to O₂ or cytochrome c of the respiratory chain (141–144). In addition to most IMS proteins, some IM and matrix proteins are also MIA-system–dependent (28, 71, 113, 114, 145).

Sorting Pathways of Mitochondrial OM Proteins

All the mitochondrial OM proteins are imported from the cytosol. The membrane contains two different types of integral protein: β-barrel proteins, which are integrated into the membrane by multiple transmembrane β-strands, and α-helical proteins, which are membrane-anchored by one or more α-helical transmembrane segments.

Sorting and Assembly Machinery for β-Barrel Proteins. The precursors of β-barrel proteins initially pass through the TOM complex at the OM (146), then bind to small TIM chaperones in the IMS (110), like the carrier precursors, to avoid aggregation (Figure 1). Subsequent insertion of the β-barrel proteins into the OM is performed by the SAM complex, which consists of a membrane-integrated protein, Sam50 (Tob55), and two peripheral membrane proteins exposed to the cytosol, Sam35 and Sam37 (147–149). Folding of the β-barrel proteins occurs at Sam50-Sam35, followed by lateral release into the lipid phase of the OM (148, 150, 151). Sam37 directly interacts with Tom22, coupling the TOM and SAM complexes into a transient supercomplex that promotes the efficient transfer of precursor proteins (54, 152).

OM Insertion of α-Helical Proteins. The main α-helical proteins are classified as signal-anchored proteins (containing an α-helical transmembrane segment at the N-terminus), tail-anchored proteins (containing an α-helical transmembrane segment at the C-terminus), and polytopic (multi-spanning) OM proteins. α-helical OM proteins are imported via distinct routes that do not involve the Tom40 channel, in contrast to most mitochondrial proteins. The insertion of some signal-anchored and polytopic OM proteins is performed by the MIM complex (153–156), which consists of multiple copies of Mim1 and one copy of Mim2, both of which are small single-spanning OM proteins (Figure 1) (157, 158). Tom70 is required for the insertion of some polytopic proteins into MIM (155, 156). In the case of tail-anchored proteins and some multi-spanning proteins, import is aided by the lipid composition of the membrane, and no proteinaceous machinery has been identified (159–162). However, the exact mechanism for sorting and insertion of α-helical outer membrane proteins is only partially understood, and further studies are urgently needed.

Integration of Mitochondrial Protein Import into Functional Networks

Mitochondrial protein import pathways not only function as independent units for protein translocation, but also are deeply integrated into a functional network of mitochondrial bioenergetics, protein quality control, mitochondrial dynamics and morphology, and interaction with other organelles.

The protein import activity serves as a sensor for the fitness and quality of mitochondria. The protein import activity is determined by the energetic state (Δψ, ATP levels) and protein homeostasis of mitochondria. Both the translocation of precursor proteins through the TIM23 complex and the TIM22 complex require the Δψ (25, 77, 78, 113, 115, 116). The ATP-dependent chaperones play essential roles in delivering carrier precursors to Tom70 receptor (47, 48), driving presequence precursor translocation to the matrix (17, 18) and folding in the matrix (92). Impairment of respiratory chain activity, reduction of ATP levels, and accumulation of misfolded proteins or reactive oxygen species (ROS) in the matrix (163) will directly affect the import-driving activity of the translocases. The protein import activity of mitochondria is thus a sensitive indicator of their energetic state and fitness.

Mitochondrial protein import machinery and respiratory chain assembly. Both the insertion of mitochondrial-encoded proteins from the matrix into the IM and the import of nuclear-encoded precursors from the cytosol into mitochondria rely heavily on the mitochondrial protein import machinery. Increased mitochondrial ROS levels generated by the respiratory chain contribute to decreased mitochondrial translation efficiency (164). In addition, the TIM23 complex forms supercomplexes with respiratory complexes III and IV as well as with the ADP/ATP carrier. These interactions of the TIM23 complex facilitate protein import under energy-limiting conditions (65, 75, 165) and can also promote the assembly of respiratory complexes (166–168). The respiratory chain complexes also function as assembly platforms for some PAM subunits (75).

Mitochondrial protein import machinery associated with protein quality control, specifically in the following aspects: (1) Mitochondrial unfolded protein response (UPR^mt): the stress-activated transcription factor ATFS-1 contains a mitochondrial presequence and a nuclear localization signal. In healthy mitochondria, ATFS-1 is imported into the mitochondrial matrix and degraded by the LON AAA+ protease. When mitochondrial import is mildly impaired, ATFS-1 is translocated into the nucleus, where it functions as a transcription factor and induces expression of mitochondrial chaperones, proteases, and other elements to promote recovery of impaired mitochondria (169–171). (2) Unfolded protein response activated by mistargeted mitochondrial proteins (UPR [am]): upon mild damage to mitochondrial protein import, some mitochondrial precursors fail to enter mitochondria and accumulate in the cytosol, thus triggering mitochondrial Precursor Over-accumulation Stress (mPOS). This is followed by a stress response termed UPR [am] that reduces cytosolic protein synthesis and increases proteasome activity to clear the mistargeted proteins from the cytosol (172, 173). (3) PTEN-induced kinase 1 (PINK1)/Parkin-induced mitophagy: in healthy conditions, PINK1 is imported into the IM by the presequence pathway and processed by MPP and the presenilin-associated rhomboid-like protease PARL, following the retrotranslocation into the cytosol and degradation by the proteasome (174, 175). Upon severe damage to mitochondrial protein import, PINK1 remains at the OM bound to the TOM complex, where it phosphorylates ubiquitin and the E3 ubiquitin ligase Parkin, triggering the removal of damaged mitochondria by mitophagy (176, 177). (4) Mitochondria as guardian in the cytosol (MAGIC): in certain conditions, some aggregation-prone or misfolded cytosolic proteins may be imported into mitochondria for further degradation. This process is termed MAGIC, suggesting a crucial role of mitochondria in cytosolic proteostasis (178). (5) Proteolysis of mitochondrial proteins: upon removal of the presequence by MPP, destabilizing N-terminal amino acid residues of the imported proteins can be further removed by the intermediate cleaving peptidase Icp55 (which removes a single amino acid) or the mitochondrial intermediate peptidase Oct1 (which removes an octapeptide) (7, 34). The matrix AAA+ proteases, CLPXP and LON, degrade misfolded proteins and prevent protein aggregation in the matrix (179–182). The IM contains two AAA+ proteases: the i-AAA protease removes misfolded proteins from the IM, IMS, and OM (183–185), whereas the m-AAA protease degrades proteins from the matrix and IM (186, 187). Thus, the process of mitochondrial protein import is connected to protein turnover and quality control.

Mitochondrial protein import machinery connected to mitochondrial membrane architecture and dynamics. The mitochondrial contact site and cristae organizing system (MICOS) is a large protein complex enriched at crista junctions of the IM (188–190). It is crucial for the maintenance of inner membrane cristae organization and is embedded into an interactional network with protein translocases, including TOM, SAM, and MIA. Thus, it provides a dynamic link between protein import, mitochondrial membrane dynamics, and membrane contact sites (136, 188, 189, 191).

The inner-membrane fusion protein optic atrophy (OPA1) is an example of how protein import and processing are connected to mitochondrial membrane dynamics. OPA1 is first processed by matrix MPP, generating a long isoform, and further processed by different IM proteases, AAA+ protease, or OMA1 in mammals and Pcp1 in yeast, yielding a short isoform (192–195). The balance between long OPA1 and the short isoform is essential for membrane fusion and fission, which is modulated by stress and mitochondrial energetic state. Thus, the processing of imported mitochondrial protein is linked to mitochondrial fragmentation, mitophagy, or even cell death.

Endoplasmic reticulum–mitochondria encounter structure (ERMES). ERMES is a multi-subunit protein complex that connects the endoplasmic reticulum and mitochondrial OM, mainly formed by the MDM complex (196, 197). Other molecules, such as voltage-dependent anion-selective channel (VDAC) (198), TOM70 (199, 200), and inositol trisphosphate (inositol 1,4,5-trisphosphate) receptors (194) also play crucial roles in forming ER-mitochondria contact sites. The outer membrane protein Mdm10 is a subunit of both SAM and MDM complexes (196). The shuttling of Mdm10 between SAM and MDM is regulated by the small protein Tom7 (58, 196, 197, 201). Therefore, TOM, SAM, and ERMES are linked as a functional network, involved in the maintenance of mitochondrial morphology and the transport of lipids and calcium (202–205).

Mitochondrial Protein Import Machinery and Heart Disease

Mitochondrial dynamics have become a key topic in the field of heart disease. However, only limited studies investigated the involvement of mitochondrial protein import machinery in these diseases (Summarized in Table 2). Moreover, most of these studies were related to the presequence pathway, which undertakes the import of ~60% of all mitochondrial proteins.

Presequence Pathway Associated With Heart Disease

TOM Complex in Heart Disease

Tom20 is an essential receptor subunit of the TOM complex that recognizes mitochondrial precursor proteins with cleavable N-terminal presequences. Tom20 expression was reduced by ischemic insults (206, 207), and showed a cardioprotective role against ischemia/reperfusion (I/R) injury through enhancing the mitochondrial import of PKCepsilon (PKCε) in an HSP90-dependent manner (208). PKCε, a member of the serine/threonine kinase family, has been demonstrated to play a protective role against cardiac I/R injury (208, 216, 293). Additionally, calcium homeostasis, which is closely related to cardiac health, is influenced by Tom20. For example, Wattamon recently reported that the protective role of fibroblast growth factor 2 (FGF-2) against calcium overload was partially mediated by mitochondrial connexin 43 (Cx43) (introduced below), probably in a Tom20-dependent manner (209). Cx43 was imported into mitochondria via a Tom20-dependent pathway (257). In another study, Tom20 was reported to be responsible for the direct insertion of VDAC, a protein crucial in regulating cardiac calcium homeostasis (210) through mitochondrial permeability transition pore (mPTP) (294) and mitochondria-associated endoplasmic reticulum membranes (MAMs), into the OM of heart mitochondria in rats (295). Thus, Tom20 could be a crucial adaptor of cardiac calcium homeostasis in a Cx43- or VDAC-associated manner.

Tom22 serves as the central receptor for both presequence precursors and carrier precursors. Furthermore, it is also the key factor linking the TOM and SAM complex to a supercomplex by interaction with Sam37, which promotes the efficient transfer of β-barrel precursors. Tom22 was recently identified as a potential receptor for cardiac mitochondrial large conductance voltage and Ca²⁺-dependent K [+] (mitoBK_Ca) channels, facilitating the import of mitoBK_Cavia the presequence pathway (211). Tom22 deficiency might induce cardiomyocyte dysfunction by interfering with cardiac mitochondrial Ca²⁺ import (211). Additionally, Tom22 has been reported as a potential regulator of heart function through assisting the synthesis of cardiac aldosterone in mitochondria (212). Finally, in chronically hypoxic rat hearts, the level of Tom22 mRNA was increased in cardiac ventricles (213), suggesting a potential role of Tom22 in ischemic heart disease.

Tom40, encoded by the TOMM40 gene, forms the main protein-conducting channel of the TOM complex. Previous studies indicated TOMM40 was genetically associated with cardiovascular-related traits (214, 215, 229, 230). Several genome-wide association studies (GWAS) have identified that the TOMM40/APOE locus was strongly associated with low-density lipoprotein cholesterol [rs157580 (231)], high-density lipoprotein cholesterol [rs2075650, (232), rs157581 (233)], high-sensitivity C-reactive protein [rs2075650 (234)], type 2 diabetes [rs157580 (231)], or metabolic syndrome (235). Additionally, experimental evidence showed that homozygous deletion of TOMM40 in mammals was lethal, and heterozygous TOMM40 knockdown mice were found to have cardiac arrhythmia that deteriorated with age (236). Upregulated Tom40 transcription was reported associated with heat stress-induced apoptosis of rat cardiomyocytes (237). Moreover, expression of Tom40 and Tim23 was reduced only in older dilated cardiomyopathy (DCM) patients but not in younger DCM patients, suggesting age-related alterations of these proteins (238).

Small Tom Proteins (Tom5, Tom6, and Tom7). An allele of TOMM5 (the gene encoding Tom5) intronic variant (rs57578064) was correlated with a significant increase in lipoprotein-associated phospholipase A2 activity, which is associated with increased risk of cardiovascular events (239). Tom7 deficit in endothelial cells particularly damaged formation of the cerebrovascular network, but not cardiac vasculature, in zebrafish and mice (296).

TIM23 Complex in Heart Disease

Tim23, the channel-forming protein of the TIM23 complex, responsible for the translocation of presequence precursors into mitochondrial matrix or IM, was reduced by hypoxia/reoxygenation (H/R) or I/R (207, 242, 243). Restoring expression of Tim23 by various treatments seemed protective (207, 242, 243) against H/R or I/R injuries. However, a controversial study from Bian showed that the protective role of zinc against I/R injury was mediated by enhanced mitophagy, accompanied by downregulation of Tom20 and Tim23 expression (244). In addition, another study reported the association of decreased Tim23 expression in patients with DCM (238).

Tim50 is the receptor of the TIM23 complex that recognizes presequence carrying proteins. Guo et al. demonstrated that the loss of Tim50 during early zebrafish embryonic development caused neurodegeneration, cardiovascular defects (dysmorphic heart, reduced heartbeat, and decreased circulating blood), and impaired motility. These pathological changes might result from increased cell death, which was mediated by mitochondrial membrane permeabilization and acceleration of cytochrome c release (240). Tang et al. further identified that Tim50 was downregulated in both human DCM heart and transverse aortic constriction (TAC)-induced murine hypertrophic heart (241). Meanwhile, global Tim50 knockout mice showed more severe cardiac hypertrophy than wild-type mice, which was alleviated by cardiac-specific overexpression of Tim50 via reducing ROS accumulation and ASK1 activity (241).

The Presequence-Pathway–Associated Chaperones in Heart Disease

mtHSP70 (also known as GRP75/ mortalin/ PBP74) is an essential ATP-dependent chaperone of the PAM complex. It drives the translocation of preproteins into the matrix in the membrane-bound motor form and exhibits typical chaperone activity to prevent protein misfolding and aggregation in the soluble form. In vitro studies, mtHSP70 was identified as a cardioprotective chaperone against H/R-induced oxidative stress (245, 246), potentially via increased import of nuclear genome-encoded antioxidant defense proteins, such as DJ-1 (246). Expression of mtHSP70 was significantly decreased in the interfibrillar mitochondria (IFM) of type 1 diabetes mellitus (T1DM) (247) and the subsarcolemmal mitochondria (SSM) of type 2 diabetes mellitus (T2DM) (248). Cardiac-specific mtHSP70 overexpression restored cardiac function and nuclear-encoded mitochondrial protein import, contributing to a beneficial impact on proteome signature and enhanced mitochondrial function during T2DM (248). Further, mtHSP70 expression was increased in myocardial samples from patients with chronic atrial fibrillation, which suggested an adaptive heat shock response to restore cellular homeostasis (249).

HSP60 and HSP10 are mitochondrial matrix chaperones, playing pivotal roles in implementing protein folding and preventing protein aggregation. Cardiac-specific HSP60 deficiency in mice led to DCM, heart failure, and lethality. Interestingly, the import of preproteins into mitochondria was unaffected by HSP60 deficiency. However, the imported proteins processed by HSP60 underwent further degradation, suggesting lower stability of those proteins (263). HSP10 showed a similar cardioprotective role in I/R-induced myocyte death (264). Both the beneficial roles of HSP60 and HSP10 in cardiomyocytes were related to the preserved function of Complex I and Complex II. Clinical evidence found upregulated expression of both mitochondrial HSP60 and HSP10 in myocardial samples from patients with chronic atrial fibrillation (265). Agsteribbe et al. reported a single case of a girl who had facial dysmorphic features and breathing difficulties upon birth and died at 2 days of age of heart failure (266). The post-mortem examination revealed that the amount of mitochondrial HSP60 was only about 1/5 of the normal level (266).

Tim14 (encoded by DNAJC19), human homolog to yeast Pam18/Tim14, is a mitochondrial IM co-chaperone of the TIM23 complex. Mutations in DNAJC19 were related to DCM and cerebellar ataxia (DCMA) syndrome, a novel autosomal recessive syndrome characterized by early-onset DCM with conduction defects, non-progressive cerebellar ataxia, testicular dysgenesis, growth failure, mild developmental delay, and 3-methylglutaconic aciduria, with or without sensorineural hearing loss and basal ganglia lesions (250–255). The pathogenic mechanism of DCMA was associated with protein import inefficiency and cardiolipin remodeling. Experimental evidence suggested DNAJC19 forms a complex with prohibitins (PHBs). Furthermore, the loss of PHB/DNAJC19 complexes affected cardiolipin acylation and led to the accumulation of cardiolipin species with altered acyl chains (297).

MAGMAS (mitochondria-associated granulocyte macrophage colony stimulating factor signaling molecule), also termed PAM16/Tim16, forms a stable subcomplex with J-protein Pam18 or DnaJC19. It tethers to the TIM23 complex in yeast and humans (298). Cybel et al. reported that two patients from a family with MAGMAS mutation died at 2 years of age of heart failure (256).

HSP90, a chaperone mainly located in the cytoplasm, also played a beneficial role against I/R injury (258, 259) through translocation of PKCε, (208, 217) Cx43, (257, 260) AKT, (261), and Pim1/Lon (262) into mitochondria, potentially via the HSP-TOM mitochondrial import pathway.

The Presequence-Pathway–Associated Proteinases and Peptidases in Heart Disease

Mitochondrial Lon Protease is crucial for the clearance of oxidized or misfolded proteins in the matrix. It played a beneficial role in improving cardiac metabolic flexibility via degradation of pyruvate dehydrogenase kinase 4 in mice fed a high-fat diet (269). Moreover, it was also verified to enhance cardiac function via improving mitochondrial respiration capacity in pressure overload-induced heart failure in mice (270). However, it seemed harmful to cardiomyocytes upon hypoxia insults, which was associated with enhanced ROS production (271, 272) and accelerated degradation of phosphorylated complex IV subunits (273). In murine heart, mitochondrial Lon protease levels rose with age, but proteolytic efficiency and adaptation to stress were compromised in older animals (274, 275). Mitochondrial Lon protease was also found to be involved in Friedreich's ataxia (FRDA), a rare hereditary neurodegenerative disease characterized by progressive ataxia and cardiomyopathy due to mitochondrial frataxin defect. In cardiac-specific frataxin-deletion mice, a progressive increase in mitochondrial Lon and ClpP protease expression and activity were found in the heart, accompanied by the loss of mitochondrial Fe–S proteins (276).

YME1L. As we mentioned above, the balance between long OPA1 (L-OPA1) and short OPA1 (S-OPA1), which is crucial for mitochondrial fusion and fission, is modulated by two mitochondrial proteases, OMA1 and the AAA+ protease YME1L. Cardiac-specific ablation of YME1L in mice led to DCM and heart failure via activated OMA1 and accelerated OPA1 proteolysis, which triggered mitochondrial fragmentation and altered cardiac metabolism (195). Moreover, cardiac function and mitochondrial morphology were rescued by Oma1 deletion by preventing OPA1 cleavage (195). The regulation of YME1L in mitochondrial fusion via OPA1 proteolysis was further verified in experimental autoimmune myocarditis animals (277) and YME1L-overexpressing or deficit cells (278). Furthermore, it was related to the therapeutic efficacy of mesenchymal stem cells for myocardial infarction (279).

MPPα. MPP is a dimeric protease in the matrix that removes N-terminal presequences and consists of MPPα and MPPβ. Mugdha reported that a patient with mutations in the PMPCA gene, which encodes MPPα, had multisystem impairments, including developmental delay, severe hypotonia, ataxia, lactic acidemia, and severe hypertrophic left ventricular cardiomyopathy and died at 14 months from respiratory failure (267). This phenotype may be related to reduced MPPα levels and impaired processing of frataxin and other mitochondrial proteins. Downregulation of MPPα was found linked to the protective role of GSK inhibitor SB216763 in I/R injury (258).

MIP/Oct1. Upon removal of the presequence by MPP, some mitochondrial precursor proteins undergo secondary processing carried out by the mitochondrial intermediate peptidase MIP/Oct1 or intermediate cleaving peptidase Icp55/XPNPEP3 to remove destabilizing N-terminal amino acid residues of the imported proteins. Mutations in the MIPEP gene, which encodes MIP, causes COXPD31/Eldomery–Sutton syndrome with developmental delay, cardiomyopathy, left ventricular non-compaction, hypotonia, and infantile death (268).

CLPP (mitochondrial ATP-dependent Clp proteolytic subunit), a mitochondrial matrix proteinase, has a central role in protein homeostasis. Loss of CLPP in the heart was found to alleviate mitochondrial cardiomyopathy induced by DARS2 deficiency, potentially mediated by increased de novo synthesis of individual OXPHOS subunits, without affecting the mammalian UPR [mt] (280).

Carrier Pathway Involved in Heart Disease

Tom70 mainly serves as the receptor for hydrophobic precursors without a cleavable presequence, such as carrier precursors. Tom70 protein was downregulated in hypertrophic heart of animals and humans, which was associated with increased oxidative stress. Furthermore, upregulation of Tom70 provided cardiomyocytes with full resistance to diverse pro-hypertrophic insults (218). Tom70 expression was also reduced by I/R insult in cardiomyocytes (219–222). Supplementation of Tom70 significantly attenuated I/R injury by promoting translocation of PKCε (216, 217) [to increase the expression of KATP channel pore-forming subunit Kir6.2 (223), augment mitochondrial respiratory capacity, and modulate cardiac glucose metabolism (224)], MICU1 (to reduce mitochondrial Ca²⁺ overload), (222) and PINK1 (associated with mitophagy) (221, 225) into mitochondria. Increased expression of PGC-1α/Tom70 was also involved in melatonin-induced cardiac protection against post-myocardial infarction, which was associated with inhibited mitochondrial impairment and reduced ROS generation (219, 220). In the hearts of diabetic db/db mice, Tom70 expression was suppressed. Reconstitution of Tom70 protected against diabetic cardiomyopathy through its antioxidant and antiapoptotic properties (226). Moreover, in aging hearts of diabetic db/db mice, only the expression of mitochondrial membrane proteins like Tom70 and VDAC, but not respiratory enzymes, could be increased by short-term exercise (227). Further, phosphoproteome mapping in a rat model of heart failure revealed phosphorylation of several import machinery proteins (Tom70, HSP90, and Tim8a), suggesting that the modification of mitochondrial protein import was involved in heart failure (228).

AGK (acylglycerol kinase). AGK is a mitochondrial lipid kinase that was recently identified as a subunit of the TIM22 complex. It plays an indispensable role in the import and assembly of mitochondrial carrier proteins in the IM (299). It has been shown that loss-of-function mutations in the AGK gene cause Sengers syndrome (281–286), an autosomal recessive mitochondrial disorder characterized by hypertrophic cardiomyopathy, congenital cataracts, skeletal myopathy, exercise intolerance, and lactic acidosis. Loss of AGK led to destabilized TIM22 complex; defects in the biogenesis of carrier substrates (such as adenine nucleotide translocator); lower complex I, III, and IV activities; perturbed tricarboxylic acid (TCA) cycle; and higher citrate synthase activity (300).

Tim8a (Tim8a/DDP1 and Tim8b/DDP2 are human homologs of yeast Tim8) is a small TIM chaperone in the IMS. Tim8a expression in ischemic rat heart was downregulated by treatment with GSK inhibitor SB216763, which showed a protective effect against I/R stress (258), suggesting a potential role of Tim8a in ischemic heart disease.

MIA Machinery Related to Heart Disease

FAD-linked sulfhydryl oxidase ALR is the human homolog of yeast Erv1. The interaction between Mia40 and Erv1/ALR facilitates the import of the small Tim proteins and cysteine-rich proteins. Inhibition of ALR activity by MitoBloCK-6 or a translation initiation codon (ATG) morpholino targeted to ALR in zebrafish embryos led to retarded cardiac development and impaired cardiac function (287).

Apoptosis-Inducing Factor (AIF) was initially characterized as a pro-apoptotic factor. It translocates from the mitochondrial IMS to the nucleus in the presence of apoptotic insults. It is also critical for the mitochondrial import and maturation of CHCHD4 (in human)/Mia40 (in yeast) (301–303). Mutations of the AIF-encoding gene AIFM1 led to early prenatal ventriculomegaly (288) and childhood cardiomyopathy (289), accompanied by respiratory chain complex I and IV deficiency. Global loss of AIF in mice during embryogenesis resulted in embryonic growth retardation and death during mid-gestation. Muscle-specific loss of AIF in mice led to severe DCM and skeletal muscle atrophy, associated with a significant defect in respiratory chain complex I activity (290). The Harlequin (Hq) mice, a genetic model with an 80% reduction in mitochondrial AIF, displayed more severe ischemic damage than wild-type hearts after acute I/R injury (291, 292).

Other Molecules Involved in Heart Disease by Impacting Mitochondrial Protein Import Efficiency or Altering Mitochondrial Protein Translocation

Cardiolipin (CL) is a unique phospholipid that is localized and synthesized in mitochondrial IM. CL plays a central role in many biological processes, such as mitochondrial biogenesis, protein import, morphology and mitophagy, oxidative phosphorylation, and apoptosis (304–306). Defective remodeling of CL due to genetic mutations of TAZ-1 causes Barth syndrome, a rare, X-linked recessive, infantile-onset debilitating disorder characterized by early-onset cardiomyopathy, skeletal myopathy, growth delay, and neutropenia (304–309). The molecular mechanisms were partially mediated by impaired mitochondrial import machinery. CL was verified to play a central role in the structural integrity and functions of mitochondrial translocases, such as TOM Complex (44, 310), TIM22 Complex, and TIM23 Complex (26, 311, 312).

Connexin 43 (Cx43) is the predominant protein forming gap junctions and non-junctional hemichannels in ventricular cardiomyocytes and is also localized at the IM of cardiomyocyte mitochondria (313–315). The translocation of Cx43 to the IM was TOM-HSP90–dependent and was enhanced by ischemic preconditioning (IP). The beneficial role of mitochondrial Cx43 in I/R stress was associated with its regulation of mitochondrial potassium influx and ROS production (257, 313–315). The cardioprotection of IP was abolished by genetic ablation of Cx43, blockade of mitochondrial Cx43 import, or age-related loss of mitochondrial Cx43 (257, 316–318).

PINK1 is imported into the mitochondrial matrix in healthy conditions, followed by retrotranslocation into cytosol and degradation by the proteasome. Perturbation of this process causes mitophagy, which plays a vital role in the quality control of mitochondria in heart disease. Given that it has been well-studied and summarized in many other reviews (319, 320), we do not discuss it here in detail.

NDUFB10, an accessory subunit of complex I, is a substrate of the MIA machinery (CHCHD4/ Mia40) for oxidation-dependent protein import into the mitochondrial IMS. Mutation of cysteine 107 of NDUFB10 impaired its mitochondrial import via CHCHD4 and resulted in complex I assembly defect, led to fatal infantile lactic acidosis and cardiomyopathy in a single-case report of an infant (321).

In addition, some other proteins also showed a protective effect against I/R or H/R stress through enhancing their translocation from the cytosol to the mitochondria; these include α-crystallin B (cryAB, the major small heat shock protein in cardiomyocytes) via VDAC-Tom20 (322) and DJ-1 via mtHSP70 (246, 323–325).

Other Conditions That Affect Mitochondrial Protein Import Efficiency in Heart Disease

According to their subcellular spatial arrangement in cardiomyocytes, mitochondria are classified into three groups: subsarcolemmal mitochondria (SSM) existing below the cell membrane, interfibrillar mitochondria (IFM) residing in rows between the myofibrils, and perinuclear mitochondria located at the nuclear poles. Mitochondrial subpopulations vary in structure and function and appear to be influenced disparately in different cardiac pathologies, including I/R, heart failure, aging, exercise, and diabetes mellitus. According to recent studies, the mitochondrial import machinery in IFM of T1DM hearts (247, 326) and SSM of T2DM hearts (248, 327) were more susceptible to inefficiency. Further, many studies reported the downregulation of mitochondrial import-machinery components in heart disease, such as heart failure, DCM, ischemic cardiomyopathy, diabetic cardiomyopathy, etc. Supplements of corresponding components could partially recover cardiac function.

However, some studies pointed out an enhancement of mitochondrial protein import in aging animals. Craig et al. found mitochondria from senescent animals exhibited a higher import rate of precursors into the matrix than mitochondria from young animals (328). Later studies showed that, although expression of some key import machinery components was upregulated in the aging heart, import efficiency was compromised (238). The mechanism and significance need to be determined in future studies.

In addition, the import rate of matrix-localized proteins was found to be increased in heart of hyperthyroid animals or by T3 treatment (329–332), which was partially mediated by elevated levels of the OM receptor Tom20 and mtHSP70. Meanwhile, the proteolysis of matrix proteins was unaffected.

Conclusion

Mitochondrial import machinery pathways are involved in various heart diseases, including heart failure, DCM, hypertrophic cardiomyopathy, ischemic cardiomyopathy, and diabetic cardiomyopathy. Mutants of genes encoding components of the mitochondrial import machinery in humans or genetic deficiency of those genes in animals usually cause severe mitochondrial cardiomyopathy and are lethal, highlighting the critical role of mitochondrial import machinery in heart disease. However, compared with neurodegenerative diseases, in which the functions of mitochondrial import machinery are relatively well-studied and established, research in heart disease is still fairly limited. Although many studies detected some components of mitochondrial import machinery, most studies simply regarded those components as indicators of mitochondrial content to evaluate mitochondrial biogenesis or mitophagy. The function of mitochondrial import machinery was highly neglected. Actually, even from the perspective of mitochondrial biogenesis or mitophagy, under different stimuli, import machinery components are able to adapt to diverse cellular functions, which are not always proportional to mitochondrial quantity.

Furthermore, our current understanding of mitochondrial import machinery in heart disease is still widely based on experimental evidence from yeast. Nevertheless, recent research in higher eukaryotes has identified more complex and diverse functions in some conserved components of mitochondrial import machinery. Furthermore, with the development of high-throughput sequencing in genomics, transcriptomics, proteomics, and metabolomics, more and more novel import machinery components have been revealed in mammals. The roles of mitochondrial import machinery in heart disease deserve considerable attention, and future studies are urgently needed.

Funding

M-HZ's lab was supported by funding from the following agencies: National Heart, Lung, and Blood Institute (HL079584, HL080499, HL089920, HL110488, HL128014, HL132500, HL137371, and HL142287) and National Cancer Institute (CA213022). M-HZ is the Eminent Scholar in Molecular and Translational Medicine of the Georgia Research Alliance.

Publisher's Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

• 1.

  Dennerlein S Wang C Rehling P . Plasticity of mitochondrial translation. Trends Cell Biol. (2017) 27:712–21. 10.1016/j.tcb.2017.05.004

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 2.

  Schmidt O Harbauer AB Rao S Eyrich B Zahedi RP Stojanovski D et al . Regulation of mitochondrial protein import by cytosolic kinases. Cell. (2011) 144:227–39. 10.1016/j.cell.2010.12.015

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 3.

  Wiedemann N Pfanner N . Mitochondrial machineries for protein import and assembly. Annu Rev Biochem. (2017) 86:685–714. 10.1146/annurev-biochem-060815-014352

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 4.

  Pfanner N Warscheid B Wiedemann N . Mitochondrial proteins: from biogenesis to functional networks. Nat Rev Mol Cell Biol. (2019) 20:267–84. 10.1038/s41580-018-0092-0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 5.

  Nicolas E Tricarico R Savage M Golemis EA Hall MJ . Disease-associated genetic variation in human mitochondrial protein import. Am J Hum Genet. (2019) 104:784–801. 10.1016/j.ajhg.2019.03.019

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 6.

  Jackson TD Palmer CS Stojanovski D . Mitochondrial diseases caused by dysfunctional mitochondrial protein import. Biochem Soc Trans. (2018) 46:1225–38. 10.1042/BST20180239

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 7.

  Vogtle FN Wortelkamp S Zahedi RP Becker D Leidhold C Gevaert K et al . Global analysis of the mitochondrial N-proteome identifies a processing peptidase critical for protein stability. Cell. (2009) 139:428–39. 10.1016/j.cell.2009.07.045

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 8.

  Roise D Horvath SJ Tomich JM Richards JH Schatz G . A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers. EMBO J. (1986) 5:1327–34. 10.1002/j.1460-2075.1986.tb04363.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 9.

  Abe Y Shodai T Muto T Mihara K Torii H Nishikawa S et al . Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20. Cell. (2000) 100:551–60. 10.1016/S0092-8674(00)80691-1

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 10.

  van Wilpe S Ryan MT Hill K Maarse AC Meisinger C Brix J et al . Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature. (1999) 401:485–9. 10.1038/46802

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 11.

  Mokranjac D Neupert W . Cell biology: architecture of a protein entry gate. Nature. (2015) 528:201–2. 10.1038/nature16318

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 12.

  Lohret TA Jensen RE Kinnally KW . Tim23, a protein import component of the mitochondrial inner membrane, is required for normal activity of the multiple conductance channel, MCC. J Cell Biol. (1997) 137:377–86. 10.1083/jcb.137.2.377

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 13.

  Bauer MF Sirrenberg C Neupert W Brunner M . Role of Tim23 as voltage sensor and presequence receptor in protein import into mitochondria. Cell. (1996) 87:33–41. 10.1016/S0092-8674(00)81320-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 14.

  Dekker PJ Keil P Rassow J Maarse AC Pfanner N Meijer M . Identification of MIM23, a putative component of the protein import machinery of the mitochondrial inner membrane. FEBS Lett. (1993) 330:66–70. 10.1016/0014-5793(93)80921-G

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 15.

  Demishtein-Zohary K Marom M Neupert W Mokranjac D Azem A . GxxxG motifs hold the TIM23 complex together. FEBS J. (2015) 282:2178–86. 10.1111/febs.13266

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 16.

  Chacinska A Lind M Frazier AE Dudek J Meisinger C Geissler A et al . Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell. (2005) 120:817–29. 10.1016/j.cell.2005.01.011

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 17.

  Kang PJ Ostermann J Shilling J Neupert W Craig EA Pfanner N . Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. Nature. (1990) 348:137–43. 10.1038/348137a0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 18.

  Horst M Oppliger W Rospert S Schonfeld HJ Schatz G Azem A . Sequential action of two hsp70 complexes during protein import into mitochondria. EMBO J. (1997) 16:1842–9. 10.1093/emboj/16.8.1842

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 19.

  Demishtein-Zohary K Gunsel U Marom M Banerjee R Neupert W Azem A et al . Role of Tim17 in coupling the import motor to the translocation channel of the mitochondrial presequence translocase. Elife. (2017) 6:22696. 10.7554/eLife.22696

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 20.

  Ting SY Yan NL Schilke BA Craig EA . Dual interaction of scaffold protein Tim44 of mitochondrial import motor with channel-forming translocase subunit Tim23. Elife. (2017) 6:23609. 10.7554/eLife.23609

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 21.

  Banerjee R Gladkova C Mapa K Witte G Mokranjac D . Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein. Elife. (2015) 4:e11897. 10.7554/eLife.11897

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 22.

  Sikor M Mapa K von Voithenberg LV Mokranjac D Lamb DC . Real-time observation of the conformational dynamics of mitochondrial Hsp70 by spFRET. EMBO J. (2013) 32:1639–49. 10.1038/emboj.2013.89

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 23.

  Schendzielorz AB Schulz C Lytovchenko O Clancy A Guiard B Ieva R et al . Two distinct membrane potential-dependent steps drive mitochondrial matrix protein translocation. J Cell Biol. (2017) 216:83–92. 10.1083/jcb.201607066

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 24.

  Schulz C Rehling P . Remodelling of the active presequence translocase drives motor-dependent mitochondrial protein translocation. Nat Commun. (2014) 5:4349. 10.1038/ncomms5349

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 25.

  Truscott KN Kovermann P Geissler A Merlin A Meijer M Driessen AJ et al . A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23. Nat Struct Biol. (2001) 8:1074–82. 10.1038/nsb726

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 26.

  Malhotra K Modak A Nangia S Daman TH Gunsel U Robinson VL et al . Cardiolipin mediates membrane and channel interactions of the mitochondrial TIM23 protein import complex receptor Tim50. Sci Adv. (2017) 3:e1700532. 10.1126/sciadv.1700532

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 27.

  Denkert N Schendzielorz AB Barbot M Versemann L Richter F Rehling P et al . Cation selectivity of the presequence translocase channel Tim23 is crucial for efficient protein import. Elife. (2017) 6:28324. 10.7554/eLife.28324

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 28.

  Ramesh A Peleh V Martinez-Caballero S Wollweber F Sommer F van der Laan M et al . A disulfide bond in the TIM23 complex is crucial for voltage gating and mitochondrial protein import. J Cell Biol. (2016) 214:417–31. 10.1083/jcb.201602074

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 29.

  Hawlitschek G Schneider H Schmidt B Tropschug M Hartl FU Neupert W . Mitochondrial protein import: identification of processing peptidase and of PEP, a processing enhancing protein. Cell. (1988) 53:795–806. 10.1016/0092-8674(88)90096-7

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 30.

  Neupert W . A perspective on transport of proteins into mitochondria: a myriad of open questions. J Mol Biol. (2015) 427:1135–58. 10.1016/j.jmb.2015.02.001

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 31.

  Fukasawa Y Tsuji J Fu SC Tomii K Horton P Imai K . MitoFates: improved prediction of mitochondrial targeting sequences and their cleavage sites. Mol Cell Proteomics. (2015) 14:1113–26. 10.1074/mcp.M114.043083

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 32.

  Varshavsky A . The N-end rule pathway and regulation by proteolysis. Protein Sci. (2011) 20:1298–345. 10.1002/pro.666

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 33.

  Veling MT Reidenbach AG Freiberger EC Kwiecien NW Hutchins PD Drahnak MJ et al . Multi-omic mitoprotease profiling defines a role for Oct1p in coenzyme Q production. Mol Cell. (2017) 68:970–77 e11. 10.1016/j.molcel.2017.11.023

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 34.

  Vogtle FN Prinz C Kellermann J Lottspeich F Pfanner N Meisinger C . Mitochondrial protein turnover: role of the precursor intermediate peptidase Oct1 in protein stabilization. Mol Biol Cell. (2011) 22:2135–43. 10.1091/mbc.e11-02-0169

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 35.

  Ieva R Schrempp SG Opalinski L Wollweber F Hoss P Heisswolf AK et al . Mgr2 functions as lateral gatekeeper for preprotein sorting in the mitochondrial inner membrane. Mol Cell. (2014) 56:641–52. 10.1016/j.molcel.2014.10.010

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 36.

  Matta SK Kumar A D'Silva P . Mgr2 regulates mitochondrial preprotein import by associating with channel-forming Tim23 subunit. Mol Biol Cell. (2020) 31:1112–23. 10.1091/mbc.E19-12-0677

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 37.

  Schendzielorz AB Bragoszewski P Naumenko N Gomkale R Schulz C Guiard B et al . Motor recruitment to the TIM23 channel's lateral gate restricts polypeptide release into the inner membrane. Nat Commun. (2018) 9:4028. 10.1038/s41467-018-06492-8

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 38.

  Stiller SB Hopker J Oeljeklaus S Schutze C Schrempp SG Vent-Schmidt J et al . Mitochondrial OXA translocase plays a major role in biogenesis of inner-membrane proteins. Cell Metab. (2016) 23:901–8. 10.1016/j.cmet.2016.04.005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 39.

  Park K Botelho SC Hong J Osterberg M Kim H . Dissecting stop transfer vs. conservative sorting pathways for mitochondrial inner membrane proteins in vivo. J Biol Chem. (2013) 288:1521–32. 10.1074/jbc.M112.409748

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 40.

  Bohnert M Rehling P Guiard B Herrmann JM Pfanner N van der Laan M . Cooperation of stop-transfer and conservative sorting mechanisms in mitochondrial protein transport. Curr Biol. (2010) 20:1227–32. 10.1016/j.cub.2010.05.058

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 41.

  Herrmann JM Neupert W Stuart RA . Insertion into the mitochondrial inner membrane of a polytopic protein, the nuclear-encoded Oxa1p. EMBO J. (1997) 16:2217–26. 10.1093/emboj/16.9.2217

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 42.

  Hell K Neupert W Stuart RA . Oxa1p acts as a general membrane insertion machinery for proteins encoded by mitochondrial DNA. EMBO J. (2001) 20:1281–8. 10.1093/emboj/20.6.1281

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 43.

  Kiebler M Pfaller R Sollner T Griffiths G Horstmann H Pfanner N et al . Identification of a mitochondrial receptor complex required for recognition and membrane insertion of precursor proteins. Nature. (1990) 348:610–6. 10.1038/348610a0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 44.

  Hill K Model K Ryan MT Dietmeier K Martin F Wagner R et al . Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins [see comment]. Nature. (1998) 395:516–21. 10.1038/26780

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 45.

  Shiota T Imai K Qiu J Hewitt VL Tan K Shen HH et al . Molecular architecture of the active mitochondrial protein gate. Science. (2015) 349:1544–8. 10.1126/science.aac6428

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 46.

  Yamano K Yatsukawa Y Esaki M Hobbs AE Jensen RE Endo T . Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. J Biol Chem. (2008) 283:3799–807. 10.1074/jbc.M708339200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 47.

  Young JC Hoogenraad NJ Hartl FU . Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70. Cell. (2003) 112:41–50. 10.1016/S0092-8674(02)01250-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 48.

  Bhangoo MK Tzankov S Fan AC Dejgaard K Thomas DY Young JC . Multiple 40-kDa heat-shock protein chaperones function in Tom70-dependent mitochondrial import. Mol Biol Cell. (2007) 18:3414–28. 10.1091/mbc.e07-01-0088

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 49.

  Wu Y Sha B . Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p. Nat Struct Mol Biol. (2006) 13:589–93. 10.1038/nsmb1106

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 50.

  Backes S Hess S Boos F Woellhaf MW Godel S Jung M et al . Tom70 enhances mitochondrial preprotein import efficiency by binding to internal targeting sequences. J Cell Biol. (2018) 217:1369–82. 10.1083/jcb.201708044

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 51.

  Melin J Kilisch M Neumann P Lytovchenko O Gomkale R Schendzielorz A et al . A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria. Biochim Biophys Acta. (2015) 1853:1850–9. 10.1016/j.bbamcr.2015.04.021

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 52.

  Yamamoto H Fukui K Takahashi H Kitamura S Shiota T Terao K et al . Roles of Tom70 in import of presequence-containing mitochondrial proteins. J Biol Chem. (2009) 284:31635–46. 10.1074/jbc.M109.041756

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 53.

  Bayrhuber M Meins T Habeck M Becker S Giller K Villinger S et al . Structure of the human voltage-dependent anion channel. Proc Natl Acad Sci USA. (2008) 105:15370–5. 10.1073/pnas.0808115105

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 54.

  Qiu J Wenz LS Zerbes RM Oeljeklaus S Bohnert M Stroud DA et al . Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. Cell. (2013) 154:596–608. 10.1016/j.cell.2013.06.033

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 55.

  Lackey SW Taylor RD Go NE Wong A Sherman EL Nargang FE . Evidence supporting the 19 beta-strand model for Tom40 from cysteine scanning and protease site accessibility studies. J Biol Chem. (2014) 289:21640–50. 10.1074/jbc.M114.578765

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 56.

  Esaki M Kanamori T Nishikawa S Shin I Schultz PG Endo T . Tom40 protein import channel binds to non-native proteins and prevents their aggregation. Nat Struct Biol. (2003) 10:988–94. 10.1038/nsb1008

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 57.

  Melin J Schulz C Wrobel L Bernhard O Chacinska A Jahn O et al . Presequence recognition by the tom40 channel contributes to precursor translocation into the mitochondrial matrix. Mol Cell Biol. (2014) 34:3473–85. 10.1128/MCB.00433-14

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 58.

  Yamano K Tanaka-Yamano S Endo T . Tom7 regulates Mdm10-mediated assembly of the mitochondrial import channel protein Tom40. J Biol Chem. (2010) 285:41222–31. 10.1074/jbc.M110.163238

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 59.

  Dietmeier K Honlinger A Bomer U Dekker PJ Eckerskorn C Lottspeich F et al . Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature. (1997) 388:195–200. 10.1038/40663

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 60.

  Gornicka A Bragoszewski P Chroscicki P Wenz LS Schulz C Rehling P et al . discrete pathway for the transfer of intermembrane space proteins across the outer membrane of mitochondria. Mol Biol Cell. (2014) 25:3999–4009. 10.1091/mbc.e14-06-1155

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 61.

  Kurz M Martin H Rassow J Pfanner N Ryan MT . Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway. Mol Biol Cell. (1999) 10:2461–74. 10.1091/mbc.10.7.2461

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 62.

  Schulz C Schendzielorz A Rehling P . Unlocking the presequence import pathway. Trends Cell Biol. (2015) 25:265–75. 10.1016/j.tcb.2014.12.001

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 63.

  Rahman B Kawano S Yunoki-Esaki K Anzai T Endo T NMR . analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain. FEBS Lett. (2014) 588:678–84. 10.1016/j.febslet.2013.12.037

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 64.

  Martinez-Caballero S Grigoriev SM Herrmann JM Campo ML Kinnally KW . Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23. J Biol Chem. (2007) 282:3584–93. 10.1074/jbc.M607551200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 65.

  van der Laan M Wiedemann N Mick DU Guiard B Rehling P Pfanner N et al . role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondria. Curr Biol. (2006) 16:2271–6. 10.1016/j.cub.2006.10.025

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 66.

  Meinecke M Wagner R Kovermann P Guiard B Mick DU Hutu DP et al . Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science. (2006) 312:1523–6. 10.1126/science.1127628

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 67.

  Tamura Y Harada Y Shiota T Yamano K Watanabe K Yokota M et al . Tim23-Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import. J Cell Biol. (2009) 184:129–41. 10.1083/jcb.200808068

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 68.

  Mokranjac D Sichting M Popov-Celeketic D Mapa K Gevorkyan-Airapetov L Zohary K et al . Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria. Mol Biol Cell. (2009) 20:1400–7. 10.1091/mbc.e08-09-0934

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 69.

  Gevorkyan-Airapetov L Zohary K Popov-Celeketic D Mapa K Hell K Neupert W et al . Interaction of Tim23 with Tim50 is essential for protein translocation by the mitochondrial TIM23 complex. J Biol Chem. (2009) 284:4865–72. 10.1074/jbc.M807041200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 70.

  Rainbolt TK Atanassova N Genereux JC Wiseman RL . Stress-regulated translational attenuation adapts mitochondrial protein import through Tim17A degradation. Cell Metab. (2013) 18:908–19. 10.1016/j.cmet.2013.11.006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 71.

  Wrobel L Sokol AM Chojnacka M Chacinska A . The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly. Sci Rep. (2016) 6:27484. 10.1038/srep27484

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 72.

  Lytovchenko O Melin J Schulz C Kilisch M Hutu DP Rehling P . Signal recognition initiates reorganization of the presequence translocase during protein import. EMBO J. (2013) 32:886–98. 10.1038/emboj.2013.23

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 73.

  Mokranjac D Popov-Celeketic D Hell K Neupert W . Role of Tim21 in mitochondrial translocation contact sites. J Biol Chem. (2005) 280:23437–40. 10.1074/jbc.C500135200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 74.

  Albrecht R Rehling P Chacinska A Brix J Cadamuro SA Volkmer R et al . The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes. EMBO Rep. (2006) 7:1233–8. 10.1038/sj.embor.7400828

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 75.

  Wiedemann N van der Laan M Hutu DP Rehling P Pfanner N . Sorting switch of mitochondrial presequence translocase involves coupling of motor module to respiratory chain. J Cell Biol. (2007) 179:1115–22. 10.1083/jcb.200709087

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 76.

  Gebert M Schrempp SG Mehnert CS Heisswolf AK Oeljeklaus S Ieva R et al . Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J Cell Biol. (2012) 197:595–604. 10.1083/jcb.201110047

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 77.

  Martin J Mahlke K Pfanner N . Role of an energized inner membrane in mitochondrial protein import. Delta psi drives the movement of presequences. J Biol Chem. (1991) 266:18051–7. 10.1016/S0021-9258(18)55235-2

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 78.

  Turakhiya U von der Malsburg K Gold VAM Guiard B Chacinska A van der Laan M et al . Protein import by the mitochondrial presequence translocase in the absence of a membrane potential. J Mol Biol. (2016) 428:1041–52. 10.1016/j.jmb.2016.01.020

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 79.

  Popov-Celeketic D Mapa K Neupert W Mokranjac D . Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria. EMBO J. (2008) 27:1469–80. 10.1038/emboj.2008.79

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 80.

  Ting SY Schilke BA Hayashi M Craig EA . Architecture of the TIM23 inner mitochondrial translocon and interactions with the matrix import motor. J Biol Chem. (2014) 289:28689–96. 10.1074/jbc.M114.588152

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 81.

  Dekker PJ Pfanner N . Role of mitochondrial GrpE and phosphate in the ATPase cycle of matrix Hsp70. J Mol Biol. (1997) 270:321–7. 10.1006/jmbi.1997.1131

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 82.

  Mokranjac D Bourenkov G Hell K Neupert W Groll M . Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor. EMBO J. (2006) 25:4675–85. 10.1038/sj.emboj.7601334

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 83.

  D'Silva PR Schilke B Hayashi M Craig EA . Interaction of the J-protein heterodimer Pam18/Pam16 of the mitochondrial import motor with the translocon of the inner membrane. Mol Biol Cell. (2008) 19:424–32. 10.1091/mbc.e07-08-0748

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 84.

  Kozany C Mokranjac D Sichting M Neupert W Hell K . The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase. Nat Struct Mol Biol. (2004) 11:234–41. 10.1038/nsmb734

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 85.

  Frazier AE Dudek J Guiard B Voos W Li Y Lind M et al . Pam16 has an essential role in the mitochondrial protein import motor. Nat Struct Mol Biol. (2004) 11:226–33. 10.1038/nsmb735

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 86.

  Li Y Dudek J Guiard B Pfanner N Rehling P Voos W . The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18. J Biol Chem. (2004) 279:38047–54. 10.1074/jbc.M404319200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 87.

  van der Laan M Chacinska A Lind M Perschil I Sickmann A Meyer HE et al . Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol Cell Biol. (2005) 25:7449–58. 10.1128/MCB.25.17.7449-7458.2005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 88.

  Taylor AB Smith BS Kitada S Kojima K Miyaura H Otwinowski Z et al . Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences. Structure. (2001) 9:615–25. 10.1016/S0969-2126(01)00621-9

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 89.

  Naamati A Regev-Rudzki N Galperin S Lill R Pines O . Dual targeting of Nfs1 and discovery of its novel processing enzyme, Icp55. J Biol Chem. (2009) 284:30200–8. 10.1074/jbc.M109.034694

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 90.

  Gakh O Cavadini P Isaya G . Mitochondrial processing peptidases. Biochim Biophys Acta. (2002) 1592:63–77. 10.1016/S0167-4889(02)00265-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 91.

  Koppen M Langer T . Protein degradation within mitochondria: versatile activities of AAA proteases and other peptidases. Crit Rev Biochem Mol Biol. (2007) 42:221–42. 10.1080/10409230701380452

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 92.

  Ostermann J Horwich AL Neupert W Hartl FU . Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis. Nature. (1989) 341:125–30. 10.1038/341125a0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 93.

  Johnson KA Bhushan S Stahl A Hallberg BM Frohn A Glaser E et al . The closed structure of presequence protease PreP forms a unique 10,000 Angstroms3 chamber for proteolysis. EMBO J. (2006) 25:1977–86. 10.1038/sj.emboj.7601080

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 94.

  Mossmann D Vogtle FN Taskin AA Teixeira PF Ring J Burkhart JM et al . Amyloid-beta peptide induces mitochondrial dysfunction by inhibition of preprotein maturation. Cell Metab. (2014) 20:662–9. 10.1016/j.cmet.2014.07.024

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 95.

  He S Fox TD . Membrane translocation of mitochondrially coded Cox2p: distinct requirements for export of N and C termini and dependence on the conserved protein Oxa1p. Mol Biol Cell. (1997) 8:1449–60. 10.1091/mbc.8.8.1449

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 96.

  Pfeffer S Woellhaf MW Herrmann JM Forster F . Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography. Nat Commun. (2015) 6:6019. 10.1038/ncomms7019

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 97.

  Ott M Herrmann JM . Co-translational membrane insertion of mitochondrially encoded proteins. Biochim Biophys Acta. (2010) 1803:767–75. 10.1016/j.bbamcr.2009.11.010

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 98.

  Hell K Herrmann JM Pratje E Neupert W Stuart RA . Oxa1p, an essential component of the N-tail protein export machinery in mitochondria. Proc Natl Acad Sci USA. (1998) 95:2250–5. 10.1073/pnas.95.5.2250

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 99.

  Hartl FU Schmidt B Wachter E Weiss H Neupert W . Transport into mitochondria and intramitochondrial sorting of the Fe/S protein of ubiquinol-cytochrome c reductase. Cell. (1986) 47:939–51. 10.1016/0092-8674(86)90809-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 100.

  Rojo EE Stuart RA Neupert W . Conservative sorting of F0-ATPase subunit 9: export from matrix requires delta pH across inner membrane and matrix ATP. EMBO J. (1995) 14:3445–51. 10.1002/j.1460-2075.1995.tb07350.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 101.

  Hildenbeutel M Theis M Geier M Haferkamp I Neuhaus HE Herrmann JM et al . The membrane insertase Oxa1 is required for efficient import of carrier proteins into mitochondria. J Mol Biol. (2012) 423:590–9. 10.1016/j.jmb.2012.07.018

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 102.

  Hansen KG Herrmann JM . Transport of proteins into mitochondria. Protein J. (2019) 38:330–42. 10.1007/s10930-019-09819-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 103.

  Endres M Neupert W Brunner M . Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM2254 complex. EMBO J. (1999) 18:3214–21. 10.1093/emboj/18.12.3214

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 104.

  Brix J Rudiger S Bukau B Schneider-Mergener J Pfanner N . Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein. J Biol Chem. (1999) 274:16522–30. 10.1074/jbc.274.23.16522

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 105.

  Faou P Hoogenraad NJ . Tom34: a cytosolic cochaperone of the Hsp90/Hsp70 protein complex involved in mitochondrial protein import. Biochim Biophys Acta. (2012) 1823:348–57. 10.1016/j.bbamcr.2011.12.001

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 106.

  Gava LM Goncalves DC Borges JC Ramos CH . Stoichiometry and thermodynamics of the interaction between the C-terminus of human 90kDa heat shock protein Hsp90 and the mitochondrial translocase of outer membrane Tom70. Arch Biochem Biophys. (2011) 513:119–25. 10.1016/j.abb.2011.06.015

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 107.

  Baker MJ Webb CT Stroud DA Palmer CS Frazier AE Guiard B et al . Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import. Mol Biol Cell. (2009) 20:769–79. 10.1091/mbc.e08-09-0903

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 108.

  Koehler CM Merchant S Oppliger W Schmid K Jarosch E Dolfini L et al . Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins. EMBO J. (1998) 17:6477–86. 10.1093/emboj/17.22.6477

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 109.

  Sirrenberg C Endres M Folsch H Stuart RA Neupert W Brunner M . Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5. Nature. (1998) 391:912–5. 10.1038/36136

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 110.

  Weinhaupl K Lindau C Hessel A Wang Y Schutze C Jores T et al . Structural basis of membrane protein chaperoning through the mitochondrial intermembrane space. Cell. (2018) 175:1365–79 e25. 10.1016/j.cell.2018.10.039

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 111.

  Sirrenberg C Bauer MF Guiard B Neupert W Brunner M . Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22. Nature. (1996) 384:582–5. 10.1038/384582a0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 112.

  Koehler CM Jarosch E Tokatlidis K Schmid K Schweyen RJ Schatz G . Import of mitochondrial carriers mediated by essential proteins of the intermembrane space. Science. (1998) 279:369–73. 10.1126/science.279.5349.369

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 113.

  Okamoto H Miyagawa A Shiota T Tamura Y Endo T . Intramolecular disulfide bond of Tim22 protein maintains integrity of the TIM22 complex in the mitochondrial inner membrane. J Biol Chem. (2014) 289:4827–38. 10.1074/jbc.M113.543264

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 114.

  Wrobel L Trojanowska A Sztolsztener ME Chacinska A . Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria. Mol Biol Cell. (2013) 24:543–54. 10.1091/mbc.e12-09-0649

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 115.

  Rehling P Model K Brandner K Kovermann P Sickmann A Meyer HE et al . Protein insertion into the mitochondrial inner membrane by a twin-pore translocase. Science. (2003) 299:1747–51. 10.1126/science.1080945

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 116.

  Kerscher O Holder J Srinivasan M Leung RS Jensen RE . The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane. J Cell Biol. (1997) 139:1663–75. 10.1083/jcb.139.7.1663

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 117.

  Li J Qian X Hu J Sha B . Molecular chaperone Hsp70/Hsp90 prepares the mitochondrial outer membrane translocon receptor Tom71 for preprotein loading. J Biol Chem. (2009) 284:23852–9. 10.1074/jbc.M109.023986

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 118.

  Wiedemann N Pfanner N Ryan MT . The three modules of ADP/ATP carrier cooperate in receptor recruitment and translocation into mitochondria. EMBO J. (2001) 20:951–60. 10.1093/emboj/20.5.951

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 119.

  Curran SP Leuenberger D Schmidt E Koehler CM . The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins. J Cell Biol. (2002) 158:1017–27. 10.1083/jcb.200205124

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 120.

  Webb CT Gorman MA Lazarou M Ryan MT Gulbis JM . Crystal structure of the mitochondrial chaperone TIM910 reveals a six-bladed alpha-propeller. Mol Cell. (2006) 21:123–33. 10.1016/j.molcel.2005.11.010

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 121.

  Curran SP Leuenberger D Oppliger W Koehler CM . The Tim9p-Tim10p complex binds to the transmembrane domains of the ADP/ATP carrier. EMBO J. (2002) 21:942–53. 10.1093/emboj/21.5.942

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 122.

  Davis AJ Alder NN Jensen RE Johnson AE . The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import. Mol Biol Cell. (2007) 18:475–86. 10.1091/mbc.e06-06-0546

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 123.

  Gebert N Chacinska A Wagner K Guiard B Koehler CM Rehling P et al . Assembly of the three small Tim proteins precedes docking to the mitochondrial carrier translocase. EMBO Rep. (2008) 9:548–54. 10.1038/embor.2008.49

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 124.

  Wagner K Gebert N Guiard B Brandner K Truscott KN Wiedemann N et al . The assembly pathway of the mitochondrial carrier translocase involves four preprotein translocases. Mol Cell Biol. (2008) 28:4251–60. 10.1128/MCB.02216-07

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 125.

  Lionaki E de Marcos Lousa C Baud C Vougioukalaki M Panayotou G Tokatlidis K . The essential function of Tim12 in vivo is ensured by the assembly interactions of its C-terminal domain. J Biol Chem. (2008) 283:15747–53. 10.1074/jbc.M800350200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 126.

  Gebert N Gebert M Oeljeklaus S von der Malsburg K Stroud DA Kulawiak B et al . Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane. Mol Cell. (2011) 44:811–8. 10.1016/j.molcel.2011.09.025

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 127.

  Kulawiak B Hopker J Gebert M Guiard B Wiedemann N Gebert N . The mitochondrial protein import machinery has multiple connections to the respiratory chain. Biochim Biophys Acta. (2013) 1827:612–26. 10.1016/j.bbabio.2012.12.004

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 128.

  Durigon R Wang Q Ceh Pavia E Grant CM Lu H . Cytosolic thioredoxin system facilitates the import of mitochondrial small Tim proteins. EMBO Rep. (2012) 13:916–22. 10.1038/embor.2012.116

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 129.

  Allen S Balabanidou V Sideris DP Lisowsky T Tokatlidis K . Erv1 mediates the Mia40-dependent protein import pathway and provides a functional link to the respiratory chain by shuttling electrons to cytochrome c. J Mol Biol. (2005) 353:937–44. 10.1016/j.jmb.2005.08.049

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 130.

  Mesecke N Terziyska N Kozany C Baumann F Neupert W Hell K et al . disulfide relay system in the intermembrane space of mitochondria that mediates protein import. Cell. (2005) 121:1059–69. 10.1016/j.cell.2005.04.011

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 131.

  Hell K . The Erv1-Mia40 disulfide relay system in the intermembrane space of mitochondria. Biochim Biophys Acta. (2008) 1783:601–9. 10.1016/j.bbamcr.2007.12.005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 132.

  Rissler M Wiedemann N Pfannschmidt S Gabriel K Guiard B Pfanner N et al . The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins. J Mol Biol. (2005) 353:485–92. 10.1016/j.jmb.2005.08.051

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 133.

  Chacinska A Pfannschmidt S Wiedemann N Kozjak V Sanjuan Szklarz LK Schulze-Specking A et al . Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins. EMBO J. (2004) 23:3735–46. 10.1038/sj.emboj.7600389

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 134.

  Terziyska N Grumbt B Kozany C Hell K . Structural and functional roles of the conserved cysteine residues of the redox-regulated import receptor Mia40 in the intermembrane space of mitochondria. J Biol Chem. (2009) 284:1353–63. 10.1074/jbc.M805035200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 135.

  Banci L Bertini I Cefaro C Cenacchi L Ciofi-Baffoni S Felli IC et al . Molecular chaperone function of Mia40 triggers consecutive induced folding steps of the substrate in mitochondrial protein import. Proc Natl Acad Sci USA. (2010) 107:20190–5. 10.1073/pnas.1010095107

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 136.

  von der Malsburg K Muller JM Bohnert M Oeljeklaus S Kwiatkowska P Becker T et al . Dual role of mitofilin in mitochondrial membrane organization and protein biogenesis. Dev Cell. (2011) 21:694–707. 10.1016/j.devcel.2011.08.026

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 137.

  Weckbecker D Longen S Riemer J Herrmann JM . Atp23 biogenesis reveals a chaperone-like folding activity of Mia40 in the IMS of mitochondria. EMBO J. (2012) 31:4348–58. 10.1038/emboj.2012.263

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 138.

  Peleh V Cordat E Herrmann JM . Mia40 is a trans-site receptor that drives protein import into the mitochondrial intermembrane space by hydrophobic substrate binding. Elife. (2016) 5:16177. 10.7554/eLife.16177

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 139.

  Milenkovic D Ramming T Muller JM Wenz LS Gebert N Schulze-Specking A et al . Identification of the signal directing Tim9 and Tim10 into the intermembrane space of mitochondria. Mol Biol Cell. (2009) 20:2530–9. 10.1091/mbc.e08-11-1108

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 140.

  Sideris DP Petrakis N Katrakili N Mikropoulou D Gallo A Ciofi-Baffoni S et al . A novel intermembrane space-targeting signal docks cysteines onto Mia40 during mitochondrial oxidative folding. J Cell Biol. (2009) 187:1007–22. 10.1083/jcb.200905134

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 141.

  Bien M Longen S Wagener N Chwalla I Herrmann JM Riemer J . Mitochondrial disulfide bond formation is driven by intersubunit electron transfer in Erv1 and proofread by glutathione. Mol Cell. (2010) 37:516–28. 10.1016/j.molcel.2010.01.017

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 142.

  Bihlmaier K Mesecke N Terziyska N Bien M Hell K Herrmann JM . The disulfide relay system of mitochondria is connected to the respiratory chain. J Cell Biol. (2007) 179:389–95. 10.1083/jcb.200707123

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 143.

  Dabir DV Leverich EP Kim SK Tsai FD Hirasawa M Knaff DB et al . role for cytochrome c and cytochrome c peroxidase in electron shuttling from Erv1. EMBO J. (2007) 26:4801–11. 10.1038/sj.emboj.7601909

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 144.

  Kawano S Yamano K Naoe M Momose T Terao K Nishikawa S et al . Structural basis of yeast Tim40/Mia40 as an oxidative translocator in the mitochondrial intermembrane space. Proc Natl Acad Sci USA. (2009) 106:14403–7. 10.1073/pnas.0901793106

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 145.

  Longen S Woellhaf MW Petrungaro C Riemer J Herrmann JM . The disulfide relay of the intermembrane space oxidizes the ribosomal subunit mrp10 on its transit into the mitochondrial matrix. Dev Cell. (2014) 28:30–42. 10.1016/j.devcel.2013.11.007

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 146.

  Jores T Klinger A Gross LE Kawano S Flinner N Duchardt-Ferner E et al . Characterization of the targeting signal in mitochondrial beta-barrel proteins. Nat Commun. (2016) 7:12036. 10.1038/ncomms12036

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 147.

  Klein A Israel L Lackey SW Nargang FE Imhof A Baumeister W et al . Characterization of the insertase for beta-barrel proteins of the outer mitochondrial membrane. J Cell Biol. (2012) 199:599–611. 10.1083/jcb.201207161

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 148.

  Wiedemann N Kozjak V Chacinska A Schonfisch B Rospert S Ryan MT et al . Machinery for protein sorting and assembly in the mitochondrial outer membrane. Nature. (2003) 424:565–71. 10.1038/nature01753

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 149.

  Paschen SA Waizenegger T Stan T Preuss M Cyrklaff M Hell K et al . Evolutionary conservation of biogenesis of beta-barrel membrane proteins. Nature. (2003) 426:862–6. 10.1038/nature02208

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 150.

  Hohr AIC Lindau C Wirth C Qiu J Stroud DA Kutik S et al . Membrane protein insertion through a mitochondrial beta-barrel gate. Science. (2018) 359:aah6834. 10.1126/science.aah6834

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 151.

  Kutik S Stojanovski D Becker L Becker T Meinecke M Kruger V et al . Dissecting membrane insertion of mitochondrial beta-barrel proteins. Cell. (2008) 132:1011–24. 10.1016/j.cell.2008.01.028

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 152.

  Wenz LS Ellenrieder L Qiu J Bohnert M Zufall N van der Laan M et al . Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting beta-barrel biogenesis. J Cell Biol. (2015) 210:1047–54. 10.1083/jcb.201504119

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 153.

  Popov-Celeketic J Waizenegger T Rapaport D . Mim1 functions in an oligomeric form to facilitate the integration of Tom20 into the mitochondrial outer membrane. J Mol Biol. (2008) 376:671–80. 10.1016/j.jmb.2007.12.006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 154.

  Hulett JM Lueder F Chan NC Perry AJ Wolynec P Likic VA et al . The transmembrane segment of Tom20 is recognized by Mim1 for docking to the mitochondrial TOM complex. J Mol Biol. (2008) 376:694–704. 10.1016/j.jmb.2007.12.021

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 155.

  Papic D Krumpe K Dukanovic J Dimmer KS Rapaport D . Multispan mitochondrial outer membrane protein Ugo1 follows a unique Mim1-dependent import pathway. J Cell Biol. (2011) 194:397–405. 10.1083/jcb.201102041

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 156.

  Becker T Wenz LS Kruger V Lehmann W Muller JM Goroncy L et al . The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins. J Cell Biol. (2011) 194:387–95. 10.1083/jcb.201102044

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 157.

  Kruger V Becker T Becker L Montilla-Martinez M Ellenrieder L Vogtle FN et al . Identification of new channels by systematic analysis of the mitochondrial outer membrane. J Cell Biol. (2017) 216:3485–95. 10.1083/jcb.201706043

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 158.

  Dimmer KS Papic D Schumann B Sperl D Krumpe K Walther DM et al . A crucial role for Mim2 in the biogenesis of mitochondrial outer membrane proteins. J Cell Sci. (2012) 125:3464–73. 10.1242/jcs.103804

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 159.

  Gupta A Becker T . Mechanisms and pathways of mitochondrial outer membrane protein biogenesis. Biochim Biophys Acta Bioenerg. (2021) 1862:148323. 10.1016/j.bbabio.2020.148323

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 160.

  Keskin A Akdogan E Dunn CD . Evidence for amino acid snorkeling from a high-resolution, in vivo analysis of Fis1 tail-anchor insertion at the mitochondrial outer membrane. Genetics. (2017) 205:691–705. 10.1534/genetics.116.196428

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 161.

  Vogtle FN Keller M Taskin AA Horvath SE Guan XL Prinz C et al . The fusogenic lipid phosphatidic acid promotes the biogenesis of mitochondrial outer membrane protein Ugo1. J Cell Biol. (2015) 210:951–60. 10.1083/jcb.201506085

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 162.

  Sauerwald J Jores T Eisenberg-Bord M Chuartzman SG Schuldiner M Rapaport D . Genome-wide screens in saccharomyces cerevisiae highlight a role for cardiolipin in biogenesis of mitochondrial outer membrane multispan proteins. Mol Cell Biol. (2015) 35:3200–11. 10.1128/MCB.00107-15

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 163.

  Haynes CM Ron D . The mitochondrial UPR - protecting organelle protein homeostasis. J Cell Sci. (2010) 123:3849–55. 10.1242/jcs.075119

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 164.

  Topf U Suppanz I Samluk L Wrobel L Boser A Sakowska P et al . Quantitative proteomics identifies redox switches for global translation modulation by mitochondrially produced reactive oxygen species. Nat Commun. (2018) 9:324. 10.1038/s41467-017-02694-8

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 165.

  Mehnert CS Rampelt H Gebert M Oeljeklaus S Schrempp SG Kochbeck L et al . The mitochondrial ADP/ATP carrier associates with the inner membrane presequence translocase in a stoichiometric manner. J Biol Chem. (2014) 289:27352–62. 10.1074/jbc.M114.556498

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 166.

  Dennerlein S Oeljeklaus S Jans D Hellwig C Bareth B Jakobs S et al . MITRAC7 acts as a COX1-specific chaperone and reveals a checkpoint during cytochrome c oxidase assembly. Cell Rep. (2015) 12:1644–55. 10.1016/j.celrep.2015.08.009

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 167.

  Mick DU Dennerlein S Wiese H Reinhold R Pacheu-Grau D Lorenzi I et al . MITRAC links mitochondrial protein translocation to respiratory-chain assembly and translational regulation. Cell. (2012) 151:1528–41. 10.1016/j.cell.2012.11.053

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 168.

  Richter-Dennerlein R Oeljeklaus S Lorenzi I Ronsor C Bareth B Schendzielorz AB et al . Mitochondrial protein synthesis adapts to influx of nuclear-encoded protein. Cell. (2016) 167:471–83 e10. 10.1016/j.cell.2016.09.003

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 169.

  Shpilka T Haynes CM . The mitochondrial UPR: mechanisms, physiological functions and implications in ageing. Nat Rev Mol Cell Biol. (2018) 19:109–20. 10.1038/nrm.2017.110

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 170.

  Nargund AM Pellegrino MW Fiorese CJ Baker BM Haynes CM . Mitochondrial import efficiency of ATFS-1 regulates mitochondrial UPR activation. Science. (2012) 337:587–90. 10.1126/science.1223560

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 171.

  Rolland SG Schneid S Schwarz M Rackles E Fischer C Haeussler S et al . Compromised mitochondrial protein import acts as a signal for UPR(mt). Cell Rep. (2019) 28:1659–69 e5. 10.1016/j.celrep.2019.07.049

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 172.

  Wrobel L Topf U Bragoszewski P Wiese S Sztolsztener ME Oeljeklaus S et al . Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol. Nature. (2015) 524:485–8. 10.1038/nature14951

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 173.

  Wang X Chen XJ . A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death. Nature. (2015) 524:481–4. 10.1038/nature14859

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 174.

  Jin SM Lazarou M Wang C Kane LA Narendra DP Youle RJ . Mitochondrial membrane potential regulates PINK1 import and proteolytic destabilization by PARL. J Cell Biol. (2010) 191:933–42. 10.1083/jcb.201008084

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 175.

  Yamano K Youle RJ . PINK1 is degraded through the N-end rule pathway. Autophagy. (2013) 9:1758–69. 10.4161/auto.24633

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 176.

  Lazarou M Jin SM Kane LA Youle RJ . Role of PINK1 binding to the TOM complex and alternate intracellular membranes in recruitment and activation of the E3 ligase Parkin. Dev Cell. (2012) 22:320–33. 10.1016/j.devcel.2011.12.014

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 177.

  Kane LA Lazarou M Fogel AI Li Y Yamano K Sarraf SA Banerjee S et al . PINK1 phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity. J Cell Biol. (2014) 205:143–53. 10.1083/jcb.201402104

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 178.

  Ruan L Zhou C Jin E Kucharavy A Zhang Y Wen Z et al . Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Nature. (2017) 543:443–46. 10.1038/nature21695

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 179.

  Wagner I Arlt H van Dyck L Langer T Neupert W . Molecular chaperones cooperate with PIM1 protease in the degradation of misfolded proteins in mitochondria. EMBO J. (1994) 13:5135–45. 10.1002/j.1460-2075.1994.tb06843.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 180.

  Kang SG Dimitrova MN Ortega J Ginsburg A Maurizi MR . Human mitochondrial ClpP is a stable heptamer that assembles into a tetradecamer in the presence of ClpX. J Biol Chem. (2005) 280:35424–32. 10.1074/jbc.M507240200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 181.

  Bender T Lewrenz I Franken S Baitzel C Voos W . Mitochondrial enzymes are protected from stress-induced aggregation by mitochondrial chaperones and the Pim1/LON protease. Mol Biol Cell. (2011) 22:541–54. 10.1091/mbc.e10-08-0718

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 182.

  Fischer F Langer JD Osiewacz HD . Identification of potential mitochondrial CLPXP protease interactors and substrates suggests its central role in energy metabolism. Sci Rep. (2015) 5:18375. 10.1038/srep18375

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 183.

  Ohba Y MacVicar T Langer T . Regulation of mitochondrial plasticity by the i-AAA protease YME1L. Biol Chem. (2020) 401:877–90. 10.1515/hsz-2020-0120

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 184.

  Baker MJ Mooga VP Guiard B Langer T Ryan MT Stojanovski D . Impaired folding of the mitochondrial small TIM chaperones induces clearance by the i-AAA protease. J Mol Biol. (2012) 424:227–39. 10.1016/j.jmb.2012.09.019

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 185.

  Wu X Li L Jiang H . Mitochondrial inner-membrane protease Yme1 degrades outer-membrane proteins Tom22 and Om45. J Cell Biol. (2018) 217:139–49. 10.1083/jcb.201702125

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 186.

  Deshwal S Fiedler KU Langer T . Mitochondrial proteases: multifaceted regulators of mitochondrial plasticity. Annu Rev Biochem. (2020) 89:501–28. 10.1146/annurev-biochem-062917-012739

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 187.

  Opalinska M Janska H . AAA proteases: guardians of mitochondrial function and homeostasis. Cells. (2018) 7:100163. 10.3390/cells7100163

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 188.

  Hoppins S Collins SR Cassidy-Stone A Hummel E Devay RM Lackner LL et al . A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria. J Cell Biol. (2011) 195:323–40. 10.1083/jcb.201107053

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 189.

  Harner M Korner C Walther D Mokranjac D Kaesmacher J Welsch U et al . The mitochondrial contact site complex, a determinant of mitochondrial architecture. EMBO J. (2011) 30:4356–70. 10.1038/emboj.2011.379

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 190.

  Pfanner N van der Laan M Amati P Capaldi RA Caudy AA Chacinska A et al . Uniform nomenclature for the mitochondrial contact site and cristae organizing system. J Cell Biol. (2014) 204:1083–6. 10.1083/jcb.201401006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 191.

  Bohnert M Wenz LS Zerbes RM Horvath SE Stroud DA von der Malsburg K et al . Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane. Mol Biol Cell. (2012) 23:3948–56. 10.1091/mbc.e12-04-0295

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 192.

  Ehses S Raschke I Mancuso G Bernacchia A Geimer S Tondera D et al . Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1. J Cell Biol. (2009) 187:1023–36. 10.1083/jcb.200906084

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 193.

  Ishihara N Fujita Y Oka T Mihara K . Regulation of mitochondrial morphology through proteolytic cleavage of OPA1. EMBO J. (2006) 25:2966–77. 10.1038/sj.emboj.7601184

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 194.

  Anand R Wai T Baker MJ Kladt N Schauss AC Rugarli E et al . The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. J Cell Biol. (2014) 204:919–29. 10.1083/jcb.201308006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 195.

  Wai T Garcia-Prieto J Baker MJ Merkwirth C Benit P Rustin P et al . Imbalanced OPA1 processing and mitochondrial fragmentation cause heart failure in mice. Science. (2015) 350:aad0116. 10.1126/science.aad0116

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 196.

  Ellenrieder L Opalinski L Becker L Kruger V Mirus O Straub SP et al . Separating mitochondrial protein assembly and endoplasmic reticulum tethering by selective coupling of Mdm10. Nat Commun. (2016) 7:13021. 10.1038/ncomms13021

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 197.

  Stroud DA Oeljeklaus S Wiese S Bohnert M Lewandrowski U Sickmann A et al . Composition and topology of the endoplasmic reticulum-mitochondria encounter structure. J Mol Biol. (2011) 413:743–50. 10.1016/j.jmb.2011.09.012

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 198.

  Muller CS Bildl W Haupt A Ellenrieder L Becker T Hunte C et al . Cryo-slicing blue native-mass spectrometry (csBN-MS), a novel technology for high resolution complexome profiling. Mol Cell Proteomics. (2016) 15:669–81. 10.1074/mcp.M115.054080

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 199.

  Elbaz-Alon Y Eisenberg-Bord M Shinder V Stiller SB Shimoni E Wiedemann N et al . Lam6 regulates the extent of contacts between organelles. Cell Rep. (2015) 12:7–14. 10.1016/j.celrep.2015.06.022

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 200.

  Murley A Sarsam RD Toulmay A Yamada J Prinz WA Nunnari J . Ltc1 is an ER-localized sterol transporter and a component of ER-mitochondria and ER-vacuole contacts. J Cell Biol. (2015) 209:539–48. 10.1083/jcb.201502033

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 201.

  Meisinger C Wiedemann N Rissler M Strub A Milenkovic D Schonfisch B et al . Mitochondrial protein sorting: differentiation of beta-barrel assembly by Tom7-mediated segregation of Mdm10. J Biol Chem. (2006) 281:22819–26. 10.1074/jbc.M602679200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 202.

  Kornmann B Currie E Collins SR Schuldiner M Nunnari J Weissman JS et al . An ER-mitochondria tethering complex revealed by a synthetic biology screen. Science. (2009) 325:477–81. 10.1126/science.1175088

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 203.

  Meisinger C Rissler M Chacinska A Szklarz LK Milenkovic D Kozjak V et al . The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane. Dev Cell. (2004) 7:61–71. 10.1016/j.devcel.2004.06.003

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 204.

  Yamano K Tanaka-Yamano S Endo T . Mdm10 as a dynamic constituent of the TOB/SAM complex directs coordinated assembly of Tom40. EMBO Rep. (2010) 11:187–93. 10.1038/embor.2009.283

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 205.

  Flinner N Ellenrieder L Stiller SB Becker T Schleiff E Mirus O . Mdm10 is an ancient eukaryotic porin co-occurring with the ERMES complex. Biochim Biophys Acta. (2013) 1833:3314–25. 10.1016/j.bbamcr.2013.10.006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 206.

  Boengler K Gres P Cabestrero A Ruiz-Meana M Garcia-Dorado D Heusch G et al . Prevention of the ischemia-induced decrease in mitochondrial Tom20 content by ischemic preconditioning. J Mol Cell Cardiol. (2006) 41:426–30. 10.1016/j.yjmcc.2006.05.015

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 207.

  Feng R Cai M Wang X Zhang J Tian Z . Early aerobic exercise combined with hydrogen-rich saline as preconditioning protects myocardial injury induced by acute myocardial infarction in rats. Appl Biochem Biotechnol. (2019) 187:663–76. 10.1007/s12010-018-2841-0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 208.

  Budas GR Churchill EN Disatnik MH Sun L Mochly-Rosen D . Mitochondrial import of PKCepsilon is mediated by HSP90: a role in cardioprotection from ischaemia and reperfusion injury. Cardiovasc Res. (2010) 88:83–92. 10.1093/cvr/cvq154

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 209.

  Srisakuldee W Makazan Z Nickel BE Zhang F Thliveris JA Pasumarthi KB et al . The FGF-2-triggered protection of cardiac subsarcolemmal mitochondria from calcium overload is mitochondrial connexin 43-dependent. Cardiovasc Res. (2014) 103:72–80. 10.1093/cvr/cvu066

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 210.

  Paillard M Tubbs E Thiebaut PA Gomez L Fauconnier J Da Silva CC et al . Depressing mitochondria-reticulum interactions protects cardiomyocytes from lethal hypoxia-reoxygenation injury. Circulation. (2013) 128:1555–65. 10.1161/CIRCULATIONAHA.113.001225

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 211.

  Zhang J Li M Zhang Z Zhu R Olcese R Stefani E et al . The mitochondrial BKCa channel cardiac interactome reveals BKCa association with the mitochondrial import receptor subunit Tom22, and the adenine nucleotide translocator. Mitochondrion. (2017) 33:84–101. 10.1016/j.mito.2016.08.017

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 212.

  Bose HS Whittal RM Marshall B Rajapaksha M Wang NP Bose M et al . A novel mitochondrial complex of aldosterone synthase, steroidogenic acute regulatory protein, and Tom22 synthesizes aldosterone in the rat heart. J Pharmacol Exp Ther. (2021) 377:108–20. 10.1124/jpet.120.000365

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 213.

  Benak D Sotakova-Kasparova D Neckar J Kolar F Hlavackova M . Selection of optimal reference genes for gene expression studies in chronically hypoxic rat heart. Mol Cell Biochem. (2019) 461:15–22. 10.1007/s11010-019-03584-x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 214.

  Middelberg RP Ferreira MA Henders AK Heath AC Madden PA Montgomery GW et al . Genetic variants in LPL, OASL and TOMM40/APOE-C1-C2-C4 genes are associated with multiple cardiovascular-related traits. BMC Med Genet. (2011) 12:123. 10.1186/1471-2350-12-123

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 215.

  Jeemon P Pettigrew K Sainsbury C Prabhakaran D Padmanabhan S . Implications of discoveries from genome-wide association studies in current cardiovascular practice. World J Cardiol. (2011) 3:230–47. 10.4330/wjc.v3.i7.230

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 216.

  Yang Z Sun W Hu K . Molecular mechanism underlying adenosine receptor-mediated mitochondrial targeting of protein kinase C. Biochim Biophys Acta. (2012) 1823:950–8. 10.1016/j.bbamcr.2011.12.012

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 217.

  Kang C Qin J Osei W Hu K . Regulation of protein kinase C-epsilon and its age-dependence. Biochem Biophys Res Commun. (2017) 482:1201–06. 10.1016/j.bbrc.2016.12.012

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 218.

  Li J Qi M Li C Shi D Zhang D Xie D et al . Tom70 serves as a molecular switch to determine pathological cardiac hypertrophy. Cell Res. (2014) 24:977–93. 10.1038/cr.2014.94

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 219.

  Pei HF Hou JN Wei FP Xue Q Zhang F Peng CF et al . Melatonin attenuates postmyocardial infarction injury via increasing Tom70 expression. J Pineal Res. (2017) 62:12371. 10.1111/jpi.12371

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 220.

  Lochner A Marais E Huisamen B . Melatonin and cardioprotection against ischaemia/reperfusion injury: what's new? A review. J Pineal Res. (2018) 65:e12490. 10.1111/jpi.12490

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 221.

  Kato H Lu Q Rapaport D Kozjak-Pavlovic V . Tom70 is essential for PINK1 import into mitochondria. PLoS ONE. (2013) 8:e58435. 10.1371/journal.pone.0058435

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 222.

  Xue Q Pei H Liu Q Zhao M Sun J Gao E et al . MICU1 protects against myocardial ischemia/reperfusion injury and its control by the importer receptor Tom70. Cell Death Dis. (2017) 8:e2923. 10.1038/cddis.2017.280

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 223.

  Garg V Hu K . Protein kinase C isoform-dependent modulation of ATP-sensitive K+ channels in mitochondrial inner membrane. Am J Physiol Heart Circ Physiol. (2007) 293:H322–32. 10.1152/ajpheart.01035.2006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 224.

  Mayr M Liem D Zhang J Li X Avliyakulov NK Yang JI et al . Proteomic and metabolomic analysis of cardioprotection: Interplay between protein kinase C epsilon and delta in regulating glucose metabolism of murine hearts. J Mol Cell Cardiol. (2009) 46:268–77. 10.1016/j.yjmcc.2008.10.008

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 225.

  Yuan Y Pan SS . Parkin mediates mitophagy to participate in cardioprotection induced by late exercise preconditioning but Bnip3 does not. J Cardiovasc Pharmacol. (2018) 71:303–16. 10.1097/FJC.0000000000000572

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 226.

  Wang P Wang D Yang Y Hou J Wan J Ran F et al . Tom70 protects against diabetic cardiomyopathy through its antioxidant and antiapoptotic properties. Hypertens Res. (2020) 43:1047–56. 10.1038/s41440-020-0518-x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 227.

  Botta A Laher I Beam J Decoffe D Brown K Halder S et al . Short term exercise induces PGC-1alpha, ameliorates inflammation and increases mitochondrial membrane proteins but fails to increase respiratory enzymes in aging diabetic hearts. PLoS ONE. (2013) 8:e70248. 10.1371/journal.pone.0070248

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 228.

  Giorgianni F Usman Khan M Weber KT Gerling IC Beranova-Giorgianni S . Phosphoproteome mapping of cardiomyocyte mitochondria in a rat model of heart failure. Mol Cell Biochem. (2014) 389:159–67. 10.1007/s11010-013-1937-7

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 229.

  Palmer ND Kahali B Kuppa A Chen Y Du X Feitosa MF et al . Allele specific variation at APOE increases non-alcoholic fatty liver disease and obesity but decreases risk of Alzheimer's disease and myocardial infarction. Hum Mol Genet. (2021) 2021:ddab096. 10.1093/hmg/ddab096

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 230.

  Talmud PJ Drenos F Shah S Shah T Palmen J Verzilli C et al . Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Am J Hum Genet. (2009) 85:628–42. 10.1016/j.ajhg.2009.10.014

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 231.

  Kong X Zhao Q Xing X Zhang B Zhang X Hong J et al . Genetic variants associated with lipid profiles in Chinese patients with type 2 diabetes. PLoS ONE. (2015) 10:e0135145. 10.1371/journal.pone.0135145

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 232.

  Abe S Tokoro F Matsuoka R Arai M Noda T Watanabe S et al . Association of genetic variants with dyslipidemia. Mol Med Rep. (2015) 12:5429–36. 10.3892/mmr.2015.4081

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 233.

  Park S Kang S . A minor allele of the haplotype located in the 19q13 loci is associated with a decreased risk of hyper-LDL-cholesterolemia, and a balanced diet and high protein intake can reduce the risk. Lipids Health Dis. (2020) 19:178. 10.1186/s12944-020-01352-1

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 234.

  Christiansen MK Larsen SB Nyegaard M Neergaard-Petersen S Ajjan R Wurtz M et al . Coronary artery disease-associated genetic variants and biomarkers of inflammation. PLoS ONE. (2017) 12:e0180365. 10.1371/journal.pone.0180365

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 235.

  Kraja AT Chasman DI North KE Reiner AP Yanek LR Kilpelainen TO et al . Pleiotropic genes for metabolic syndrome and inflammation. Mol Genet Metab. (2014) 112:317–38. 10.1016/j.ymgme.2014.04.007

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 236.

  Zeh R . Neurological and Molecular Biological Characterisation of the Mutant Mouse Line Tom40. Munich: Technische Universität München. (2013).

  • Google Scholar

• 237.

  Wang X Wang S Liu W Wang T Wang J Gao X et al . Epigenetic upregulation of miR-126 induced by heat stress contributes to apoptosis of rat cardiomyocytes by promoting Tomm40 transcription. J Mol Cell Cardiol. (2019) 129:39–48. 10.1016/j.yjmcc.2018.10.005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 238.

  Barcena ML Pozdniakova S Haritonow N Breiter P Kuhl AA Milting H et al . Dilated cardiomyopathy impairs mitochondrial biogenesis and promotes inflammation in an age- and sex-dependent manner. Aging. (2020) 12:24117–33. 10.18632/aging.202283

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 239.

  Yeo A Li L Warren L Aponte J Fraser D King K et al . Pharmacogenetic meta-analysis of baseline risk factors, pharmacodynamic, efficacy and tolerability endpoints from two large global cardiovascular outcomes trials for darapladib. PLoS ONE. (2017) 12:e0182115. 10.1371/journal.pone.0182115

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 240.

  Guo Y Cheong N Zhang Z De Rose R Deng Y Farber SA et al . Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. J Biol Chem. (2004) 279:24813–25. 10.1074/jbc.M402049200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 241.

  Tang K Zhao Y Li H Zhu M Li W Liu W et al . Translocase of inner membrane 50 functions as a novel protective regulator of pathological cardiac hypertrophy. J Am Heart Assoc. (2017) 6:4346. 10.1161/JAHA.116.004346

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 242.

  Zhang XX Wu XS Mi SH Fang SJ Liu S Xin Y et al . Neuregulin-1 promotes mitochondrial biogenesis, attenuates mitochondrial dysfunction, and prevents hypoxia/reoxygenation injury in neonatal cardiomyocytes. Cell Biochem Funct. (2020) 38:549–57. 10.1002/cbf.3503

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 243.

  Schubert C Raparelli V Westphal C Dworatzek E Petrov G Kararigas G et al . Reduction of apoptosis and preservation of mitochondrial integrity under ischemia/reperfusion injury is mediated by estrogen receptor beta. Biol Sex Differ. (2016) 7:53. 10.1186/s13293-016-0104-8

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 244.

  Bian X Teng T Zhao H Qin J Qiao Z Sun Y et al . Zinc prevents mitochondrial superoxide generation by inducing mitophagy in the setting of hypoxia/reoxygenation in cardiac cells. Free Radic Res. (2018) 52:80–91. 10.1080/10715762.2017.1414949

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 245.

  Williamson CL Dabkowski ER Dillmann WH Hollander JM . Mitochondria protection from hypoxia/reoxygenation injury with mitochondria heat shock protein 70 overexpression. Am J Physiol Heart Circ Physiol. (2008) 294:H249–56. 10.1152/ajpheart.00775.2007

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 246.

  Zhou TT Wang XY Huang J Deng YZ Qiu LJ Liu HY et al . Mitochondrial translocation of DJ-1 is mediated by Grp75: implication in cardioprotection of resveratrol against hypoxia/reoxygenation-induced oxidative stress. J Cardiovasc Pharmacol. (2020) 75:305–13. 10.1097/FJC.0000000000000805

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 247.

  Baseler WA Dabkowski ER Williamson CL Croston TL Thapa D Powell MJ et al . Proteomic alterations of distinct mitochondrial subpopulations in the type 1 diabetic heart: contribution of protein import dysfunction. Am J Physiol Regul Integr Comp Physiol. (2011) 300:R186–200. 10.1152/ajpregu.00423.2010

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 248.

  Shepherd DL Hathaway QA Nichols CE Durr AJ Pinti MV Hughes KM et al . Mitochondrial proteome disruption in the diabetic heart through targeted epigenetic regulation at the mitochondrial heat shock protein 70 (mtHsp70) nuclear locus. J Mol Cell Cardiol. (2018) 119:104–15. 10.1016/j.yjmcc.2018.04.016

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 249.

  Kirmanoglou K Hannekum A Schafler AE . Expression of mortalin in patients with chronic atrial fibrillation. Basic Res Cardiol. (2004) 99:404–8. 10.1007/s00395-004-0477-4

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 250.

  Davey KM Parboosingh JS McLeod DR Chan A Casey R Ferreira P et al . Mutation of DNAJC19, a human homologue of yeast inner mitochondrial membrane co-chaperones, causes DCMA syndrome, a novel autosomal recessive Barth syndrome-like condition. J Med Genet. (2006) 43:385–93. 10.1136/jmg.2005.036657

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 251.

  Ojala T Polinati P Manninen T Hiippala A Rajantie J Karikoski R et al . New mutation of mitochondrial DNAJC19 causing dilated and noncompaction cardiomyopathy, anemia, ataxia, and male genital anomalies. Pediatr Res. (2012) 72:432–7. 10.1038/pr.2012.92

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 252.

  Ucar SK Mayr JA Feichtinger RG Canda E Coker M Wortmann SB . Previously unreported biallelic mutation in DNAJC19: are sensorineural hearing loss and basal ganglia lesions additional features of dilated cardiomyopathy and ataxia (DCMA) syndrome?JIMD Rep. (2017) 35:39–45. 10.1007/8904_2016_23

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 253.

  Al Teneiji A Siriwardena K George K Mital S Mercimek-Mahmutoglu S . Progressive cerebellar atrophy and a novel homozygous pathogenic DNAJC19 variant as a cause of dilated cardiomyopathy ataxia syndrome. Pediatr Neurol. (2016) 62:58–61. 10.1016/j.pediatrneurol.2016.03.020

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 254.

  Sparkes R Patton D Bernier F . Cardiac features of a novel autosomal recessive dilated cardiomyopathic syndrome due to defective importation of mitochondrial protein. Cardiol Young. (2007) 17:215–7. 10.1017/S1047951107000042

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 255.

  Vasilescu C Ojala TH Brilhante V Ojanen S Hinterding HM Palin E et al . Genetic basis of severe childhood-onset cardiomyopathies. J Am Coll Cardiol. (2018) 72:2324–38. 10.1016/j.jacc.2018.08.2171

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 256.

  Mehawej C Delahodde A Legeai-Mallet L Delague V Kaci N Desvignes JP et al . The impairment of MAGMAS function in human is responsible for a severe skeletal dysplasia. PLoS Genet. (2014) 10:e1004311. 10.1371/journal.pgen.1004311

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 257.

  Rodriguez-Sinovas A Boengler K Cabestrero A Gres P Morente M Ruiz-Meana M et al . Translocation of connexin 43 to the inner mitochondrial membrane of cardiomyocytes through the heat shock protein 90-dependent TOM pathway and its importance for cardioprotection. Circ Res. (2006) 99:93–101. 10.1161/01.RES.0000230315.56904.de

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 258.

  Nguyen T Wong R Wang G Gucek M Steenbergen C Murphy E . Acute inhibition of GSK causes mitochondrial remodeling. Am J Physiol Heart Circ Physiol. (2012) 302:H2439–45. 10.1152/ajpheart.00033.2012

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 259.

  Small BA Lu Y Hsu AK Gross GJ Gross ER . Morphine reduces myocardial infarct size via heat shock protein 90 in rodents. Biomed Res Int. (2015) 2015:129612. 10.1155/2015/129612

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 260.

  Tu RH Li QJ Huang Z He Y Meng JJ Zheng HL Zeng ZY et al . Novel functional role of heat shock protein 90 in mitochondrial connexin 43-mediated hypoxic postconditioning. Cell Physiol Biochem. (2017) 44:982–97. 10.1159/000485399

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 261.

  Barksdale KA Bijur GN . The basal flux of Akt in the mitochondria is mediated by heat shock protein 90. J Neurochem. (2009) 108:1289–99. 10.1111/j.1471-4159.2009.05878.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 262.

  Borillo GA Mason M Quijada P Volkers M Cottage C McGregor M et al . Pim-1 kinase protects mitochondrial integrity in cardiomyocytes. Circ Res. (2010) 106:1265–74. 10.1161/CIRCRESAHA.109.212035

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 263.

  Fan F Duan Y Yang F Trexler C Wang H Huang L et al . Deletion of heat shock protein 60 in adult mouse cardiomyocytes perturbs mitochondrial protein homeostasis and causes heart failure. Cell Death Differ. (2020) 27:587–600. 10.1038/s41418-019-0374-x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 264.

  Lin KM Hollander JM Kao VY Lin B Macpherson L Dillmann WH . Myocyte protection by 10 kD heat shock protein (Hsp10) involves the mobile loop and attenuation of the Ras GTP-ase pathway. FASEB J. (2004) 18:1004–6. 10.1096/fj.03-0348fje

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 265.

  Schafler AE Kirmanoglou K Pecher P Hannekum A Schumacher B . Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation. Ann Thorac Surg. (2002) 74:767–70. 10.1016/S0003-4975(02)03830-4

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 266.

  Agsteribbe E Huckriede A Veenhuis M Ruiters MH Niezen-Koning KE Skjeldal OH et al . A fatal, systemic mitochondrial disease with decreased mitochondrial enzyme activities, abnormal ultrastructure of the mitochondria and deficiency of heat shock protein 60. Biochem Biophys Res Commun. (1993) 193:146–54. 10.1006/bbrc.1993.1602

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 267.

  Joshi M Anselm I Shi J Bale TA Towne M Schmitz-Abe K et al . Mutations in the substrate binding glycine-rich loop of the mitochondrial processing peptidase-alpha protein (PMPCA) cause a severe mitochondrial disease. Cold Spring Harb Mol Case Stud. (2016) 2:a000786. 10.1101/mcs.a000786

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 268.

  Eldomery MK Akdemir ZC Vogtle FN Charng WL Mulica P Rosenfeld JA et al . MIPEP recessive variants cause a syndrome of left ventricular non-compaction, hypotonia, and infantile death. Genome Med. (2016) 8:106. 10.1186/s13073-016-0360-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 269.

  Crewe C Schafer C Lee I Kinter M Szweda LI . Regulation of pyruvate dehydrogenase kinase 4 in the heart through degradation by the lon protease in response to mitochondrial substrate availability. J Biol Chem. (2017) 292:305–12. 10.1074/jbc.M116.754127

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 270.

  Hoshino A Okawa Y Ariyoshi M Kaimoto S Uchihashi M Fukai K et al . Oxidative post-translational modifications develop LONP1 dysfunction in pressure overload heart failure. Circ Heart Fail. (2014) 7:500–9. 10.1161/CIRCHEARTFAILURE.113.001062

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 271.

  Zhan R Guo W Gao X Liu X Xu K Tang B . Real-time in situ monitoring of Lon and Caspase-3 for assessing the state of cardiomyocytes under hypoxic conditions via a novel Au-Se fluorescent nanoprobe. Biosens Bioelectron. (2021) 176:112965. 10.1016/j.bios.2021.112965

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 272.

  Kuo CY Chiu YC Lee AY Hwang TL . Mitochondrial Lon protease controls ROS-dependent apoptosis in cardiomyocyte under hypoxia. Mitochondrion. (2015) 23:7–16. 10.1016/j.mito.2015.04.004

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 273.

  Sepuri NBV Angireddy R Srinivasan S Guha M Spear J Lu B et al . Mitochondrial LON protease-dependent degradation of cytochrome c oxidase subunits under hypoxia and myocardial ischemia. Biochim Biophys Acta Bioenerg. (2017) 1858:519–28. 10.1016/j.bbabio.2017.04.003

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 274.

  Pomatto LCD Cline M Woodward N Pakbin P Sioutas C Morgan TE et al . Aging attenuates redox adaptive homeostasis and proteostasis in female mice exposed to traffic-derived nanoparticles (“vehicular smog”). Free Radic Biol Med. (2018) 121:86–97. 10.1016/j.freeradbiomed.2018.04.574

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 275.

  Delaval E Perichon M Friguet B . Age-related impairment of mitochondrial matrix aconitase and ATP-stimulated protease in rat liver and heart. Eur J Biochem. (2004) 271:4559–64. 10.1111/j.1432-1033.2004.04422.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 276.

  Guillon B Bulteau AL Wattenhofer-Donze M Schmucker S Friguet B Puccio H et al . Frataxin deficiency causes upregulation of mitochondrial Lon and ClpP proteases and severe loss of mitochondrial Fe-S proteins. FEBS J. (2009) 276:1036–47. 10.1111/j.1742-4658.2008.06847.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 277.

  Wu B Li J Ni H Zhuang X Qi Z Chen Q et al . TLR4 activation promotes the progression of experimental autoimmune myocarditis to dilated cardiomyopathy by inducing mitochondrial dynamic imbalance. Oxid Med Cell Longev. (2018) 2018:3181278. 10.1155/2018/3181278

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 278.

  Hu Y Xu Y Chen W Qiu Z . Stomatin-like protein-2: a potential target to treat mitochondrial cardiomyopathy. Heart Lung Circ. (2021) 5:74. 10.1016/j.hlc.2021.05.074

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 279.

  Zhao Y Zhu J Zhang N Liu Q Wang Y Hu X et al . GDF11 enhances therapeutic efficacy of mesenchymal stem cells for myocardial infarction via YME1L-mediated OPA1 processing. Stem Cells Transl Med. (2020) 9:1257–71. 10.1002/sctm.20-0005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 280.

  Seiferling D Szczepanowska K Becker C Senft K Hermans S Maiti P et al . Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt. EMBO Rep. (2016) 17:953–64. 10.15252/embr.201642077

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 281.

  Mayr JA Haack TB Graf E Zimmermann FA Wieland T Haberberger B et al . Lack of the mitochondrial protein acylglycerol kinase causes Sengers syndrome. Am J Hum Genet. (2012) 90:314–20. 10.1016/j.ajhg.2011.12.005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 282.

  Allali S Dorboz I Samaan S Slama A Rambaud C Boespflug-Tanguy O et al . Mutation in the AGK gene in two siblings with unusual Sengers syndrome. Metab Brain Dis. (2017) 32:2149–54. 10.1007/s11011-017-0101-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 283.

  Khatter S Puri RD Bijarnia-Mahay S Aggarwal M Ramprasad V Saxena R et al . Sengers syndrome in Asian Indians–two novel mutations and variant phenotype-genotype correlation. Transl Sci Rare Dis. (2017) 2:157–64. 10.3233/TRD-170017

  • CrossRef
  • Google Scholar

• 284.

  Haghighi A Haack TB Atiq M Mottaghi H Haghighi-Kakhki H Bashir RA et al . Sengers syndrome: six novel AGK mutations in seven new families and review of the phenotypic and mutational spectrum of 29 patients. Orphanet J Rare Dis. (2014) 9:119. 10.1186/s13023-014-0119-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 285.

  Kang Y Stroud DA Baker MJ De Souza DP Frazier AE Liem M et al . Sengers syndrome-associated mitochondrial acylglycerol kinase is a subunit of the human TIM22 protein import complex. Mol Cell. (2017) 67:457–70 e5. 10.1016/j.molcel.2017.06.014

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 286.

  Kor D Yilmaz B Horoz O Ceylaner G Sizmaz S Demir F . Two novel mutations in the AGK gene: two case reports with Sengers syndrome. Gene Technol. (2016) 5:2. 10.4172/2329-6682.1000140

  • CrossRef
  • Google Scholar

• 287.

  Dabir DV Hasson SA Setoguchi K Johnson ME Wongkongkathep P Douglas CJ et al . A small molecule inhibitor of redox-regulated protein translocation into mitochondria. Dev Cell. (2013) 25:81–92. 10.1016/j.devcel.2013.03.006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 288.

  Berger I Ben-Neriah Z Dor-Wolman T Shaag A Saada A Zenvirt S et al . Early prenatal ventriculomegaly due to an AIFM1 mutation identified by linkage analysis and whole exome sequencing. Mol Genet Metab. (2011) 104:517–20. 10.1016/j.ymgme.2011.09.020

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 289.

  Heimer G Eyal E Zhu X Ruzzo EK Marek-Yagel D Sagiv D et al . Mutations in AIFM1 cause an X-linked childhood cerebellar ataxia partially responsive to riboflavin. Eur J Paediatr Neurol. (2018) 22:93–101. 10.1016/j.ejpn.2017.09.004

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 290.

  Joza N Oudit GY Brown D Benit P Kassiri Z Vahsen N et al . Muscle-specific loss of apoptosis-inducing factor leads to mitochondrial dysfunction, skeletal muscle atrophy, and dilated cardiomyopathy. Mol Cell Biol. (2005) 25:10261–72. 10.1128/MCB.25.23.10261-10272.2005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 291.

  van Empel VP Bertrand AT van der Nagel R Kostin S Doevendans PA Crijns HJ et al . Downregulation of apoptosis-inducing factor in harlequin mutant mice sensitizes the myocardium to oxidative stress-related cell death and pressure overload-induced decompensation. Circ Res. (2005) 96:e92–e101. 10.1161/01.RES.0000172081.30327.28

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 292.

  Xu A Szczepanek K Hu Y Lesnefsky EJ Chen Q . Cardioprotection by modulation of mitochondrial respiration during ischemia-reperfusion: role of apoptosis-inducing factor. Biochem Biophys Res Commun. (2013) 435:627–33. 10.1016/j.bbrc.2013.05.033

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 293.

  Chen L Shi D Guo M . The roles of PKC-delta and PKC-epsilon in myocardial ischemia/reperfusion injury. Pharmacol Res. (2021) 170:105716. 10.1016/j.phrs.2021.105716

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 294.

  Javadov S Karmazyn M Escobales N . Mitochondrial permeability transition pore opening as a promising therapeutic target in cardiac diseases. J Pharmacol Exp Ther. (2009) 330:670–8. 10.1124/jpet.109.153213

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 295.

  Schleiff E Silvius JR Shore GC . Direct membrane insertion of voltage-dependent anion-selective channel protein catalyzed by mitochondrial Tom20. J Cell Biol. (1999) 145:973–8. 10.1083/jcb.145.5.973

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 296.

  Shi D Qi M Zhou L Li X Ni L Li C et al . Endothelial mitochondrial preprotein translocase Tomm7-Rac1 signaling axis dominates cerebrovascular network homeostasis. Arterioscler Thromb Vasc Biol. (2018) 38:2665–77. 10.1161/ATVBAHA.118.311538

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 297.

  Richter-Dennerlein R Korwitz A Haag M Tatsuta T Dargazanli S Baker M et al . DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. Cell Metab. (2014) 20:158–71. 10.1016/j.cmet.2014.04.016

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 298.

  Sinha D Joshi N Chittoor B Samji P D'Silva P . Role of Magmas in protein transport and human mitochondria biogenesis. Hum Mol Genet. (2010) 19:1248–62. 10.1093/hmg/ddq002

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 299.

  Vukotic M Nolte H Konig T Saita S Ananjew M Kruger M et al . Acylglycerol kinase mutated in sengers syndrome is a subunit of the TIM22 protein translocase in mitochondria. Mol Cell. (2017) 67:471–83 e7. 10.1016/j.molcel.2017.06.013

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 300.

  Siriwardena K Mackay N Levandovskiy V Blaser S Raiman J Kantor PF et al . Mitochondrial citrate synthase crystals: novel finding in Sengers syndrome caused by acylglycerol kinase (AGK) mutations. Mol Genet Metab. (2013) 108:40–50. 10.1016/j.ymgme.2012.11.282

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 301.

  Hangen E Feraud O Lachkar S Mou H Doti N Fimia GM et al . Interaction between AIF and CHCHD4 regulates respiratory chain biogenesis. Mol Cell. (2015) 58:1001–14. 10.1016/j.molcel.2015.04.020

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 302.

  Meyer K Buettner S Ghezzi D Zeviani M Bano D Nicotera P . Loss of apoptosis-inducing factor critically affects MIA40 function. Cell Death Dis. (2015) 6:e1814. 10.1038/cddis.2015.170

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 303.

  Modjtahedi N Kroemer G . CHCHD4 links AIF to the biogenesis of respiratory chain complex I. Mol Cell Oncol. (2016) 3:e1074332. 10.1080/23723556.2015.1074332

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 304.

  Bertero E Kutschka I Maack C Dudek J . Cardiolipin remodeling in Barth syndrome and other hereditary cardiomyopathies. Biochim Biophys Acta Mol Basis Dis. (2020) 1866:165803. 10.1016/j.bbadis.2020.165803

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 305.

  Monteiro JP Oliveira PJ Jurado AS . Mitochondrial membrane lipid remodeling in pathophysiology: a new target for diet and therapeutic interventions. Prog Lipid Res. (2013) 52:513–28. 10.1016/j.plipres.2013.06.002

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 306.

  Paradies G Paradies V Ruggiero FM Petrosillo G . Role of cardiolipin in mitochondrial function and dynamics in health and disease: molecular and pharmacological aspects. Cells. (2019) 8:70728. 10.3390/cells8070728

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 307.

  Brandner K Mick DU Frazier AE Taylor RD Meisinger C Rehling P . Taz1, an outer mitochondrial membrane protein, affects stability and assembly of inner membrane protein complexes: implications for Barth Syndrome. Mol Biol Cell. (2005) 16:5202–14. 10.1091/mbc.e05-03-0256

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 308.

  Sabbah HN . Barth syndrome cardiomyopathy: targeting the mitochondria with elamipretide. Heart Fail Rev. (2021) 26:237–53. 10.1007/s10741-020-10031-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 309.

  Vreken P Valianpour F Nijtmans LG Grivell LA Plecko B Wanders RJ et al . Defective remodeling of cardiolipin and phosphatidylglycerol in Barth syndrome. Biochem Biophys Res Commun. (2000) 279:378–82. 10.1006/bbrc.2000.3952

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 310.

  Gebert N Joshi AS Kutik S Becker T McKenzie M Guan XL et al . Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndrome. Curr Biol. (2009) 19:2133–9. 10.1016/j.cub.2009.10.074

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 311.

  Kutik S Rissler M Guan XL Guiard B Shui G Gebert N et al . The translocator maintenance protein Tam41 is required for mitochondrial cardiolipin biosynthesis. J Cell Biol. (2008) 183:1213–21. 10.1083/jcb.200806048

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 312.

  Tamura Y Harada Y Yamano K Watanabe K Ishikawa D Ohshima C et al . Identification of Tam41 maintaining integrity of the TIM23 protein translocator complex in mitochondria. J Cell Biol. (2006) 174:631–7. 10.1083/jcb.200603087

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 313.

  Boengler K Schulz R . Connexin 43 and mitochondria in cardiovascular health and disease. Adv Exp Med Biol. (2017) 982:227–46. 10.1007/978-3-319-55330-6_12

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 314.

  Michela P Velia V Aldo P Ada P . Role of connexin 43 in cardiovascular diseases. Eur J Pharmacol. (2015) 768:71–6. 10.1016/j.ejphar.2015.10.030

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 315.

  Wang M Smith K Yu Q Miller C Singh K Sen CK . Mitochondrial connexin 43 in sex-dependent myocardial responses and estrogen-mediated cardiac protection following acute ischemia/reperfusion injury. Basic Res Cardiol. (2019) 115:1. 10.1007/s00395-019-0759-5

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 316.

  Boengler K Konietzka I Buechert A Heinen Y Garcia-Dorado D Heusch G et al . Loss of ischemic preconditioning's cardioprotection in aged mouse hearts is associated with reduced gap junctional and mitochondrial levels of connexin 43. Am J Physiol Heart Circ Physiol. (2007) 292:H1764–9. 10.1152/ajpheart.01071.2006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 317.

  Schulz R Boengler K Totzeck A Luo Y Garcia-Dorado D Heusch G . Connexin 43 in ischemic pre- and postconditioning. Heart Fail Rev. (2007) 12:261–6. 10.1007/s10741-007-9032-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 318.

  Ruiz-Meana M Rodriguez-Sinovas A Cabestrero A Boengler K Heusch G Garcia-Dorado D . Mitochondrial connexin43 as a new player in the pathophysiology of myocardial ischaemia-reperfusion injury. Cardiovasc Res. (2008) 77:325–33. 10.1093/cvr/cvm062

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 319.

  Saito T Sadoshima J . Molecular mechanisms of mitochondrial autophagy/mitophagy in the heart. Circ Res. (2015) 116:1477–90. 10.1161/CIRCRESAHA.116.303790

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 320.

  Mukherjee UA Ong SB Ong SG Hausenloy DJ . Parkinson's disease proteins: novel mitochondrial targets for cardioprotection. Pharmacol Ther. (2015) 156:34–43. 10.1016/j.pharmthera.2015.10.005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 321.

  Friederich MW Erdogan AJ Coughlin CR Elos MT Jiang H O'Rourke CP et al . Mutations in the accessory subunit NDUFB10 result in isolated complex I deficiency and illustrate the critical role of intermembrane space import for complex I holoenzyme assembly. Hum Mol Genet. (2017) 26:702–16. 10.1093/hmg/ddw431

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 322.

  Chis R Sharma P Bousette N Miyake T Wilson A Backx PH et al . alpha-Crystallin B prevents apoptosis after H2O2 exposure in mouse neonatal cardiomyocytes. Am J Physiol Heart Circ Physiol. (2012) 303:H967–78. 10.1152/ajpheart.00040.2012

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 323.

  Zhang Y Li XR Zhao L Duan GL Xiao L Chen HP . DJ-1 preserving mitochondrial complex I activity plays a critical role in resveratrol-mediated cardioprotection against hypoxia/reoxygenation-induced oxidative stress. Biomed Pharmacother. (2018) 98:545–52. 10.1016/j.biopha.2017.12.094

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 324.

  Deng YZ Xiao L Zhao L Qiu LJ Ma ZX Xu XW et al . Molecular mechanism underlying hypoxic preconditioning-promoted mitochondrial translocation of DJ-1 in hypoxia/reoxygenation H9c2 cells. Molecules. (2019) 25:10071. 10.3390/molecules25010071

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 325.

  Ding H Xu XW Wang H Xiao L Zhao L Duan GL et al . DJ-1 plays an obligatory role in the cardioprotection of delayed hypoxic preconditioning against hypoxia/reoxygenation-induced oxidative stress through maintaining mitochondrial complex I activity. Cell Biochem Funct. (2018) 36:147–54. 10.1002/cbf.3326

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 326.

  Baseler WA Dabkowski ER Jagannathan R Thapa D Nichols CE Shepherd DL et al . Reversal of mitochondrial proteomic loss in Type 1 diabetic heart with overexpression of phospholipid hydroperoxide glutathione peroxidase. Am J Physiol Regul Integr Comp Physiol. (2013) 304:R553–65. 10.1152/ajpregu.00249.2012

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 327.

  Dabkowski ER Baseler WA Williamson CL Powell M Razunguzwa TT Frisbee JC et al . Mitochondrial dysfunction in the type 2 diabetic heart is associated with alterations in spatially distinct mitochondrial proteomes. Am J Physiol Heart Circ Physiol. (2010) 299:H529–40. 10.1152/ajpheart.00267.2010

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 328.

  Craig EE Hood DA . Influence of aging on protein import into cardiac mitochondria. Am J Physiol. (1997) 272:H2983–8. 10.1152/ajpheart.1997.272.6.H2983

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 329.

  Schneider JJ Hood DA . Effect of thyroid hormone on mtHsp70 expression, mitochondrial import and processing in cardiac muscle. J Endocrinol. (2000) 165:9–17. 10.1677/joe.0.1650009

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 330.

  Marin-Garcia J . Thyroid hormone and myocardial mitochondrial biogenesis. Vascul Pharmacol. (2010) 52:120–30. 10.1016/j.vph.2009.10.008

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 331.

  Craig EE Chesley A Hood DA . Thyroid hormone modifies mitochondrial phenotype by increasing protein import without altering degradation. Am J Physiol. (1998) 275:C1508–15. 10.1152/ajpcell.1998.275.6.C1508

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 332.

  Colavecchia M Christie LN Kanwar YS Hood DA . Functional consequences of thyroid hormone-induced changes in the mitochondrial protein import pathway. Am J Physiol Endocrinol Metab. (2003) 284:E29–35. 10.1152/ajpendo.00294.2002

  • Pubmed Abstract
  • CrossRef
  • Google Scholar