AUTHOR=Guan Ruicong , Zeng Kuan , Zhang Bin , Gao Minnan , Li Jianfen , Jiang Huiqi , Liu Yuqiang , Qiang Yongjia , Liu Zhuxuan , Li Jingwen , Yang Yanqi 

TITLE=Plasma Exosome miRNAs Profile in Patients With ST-Segment Elevation Myocardial Infarction

JOURNAL=Frontiers in Cardiovascular Medicine

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2022.848812

DOI=10.3389/fcvm.2022.848812

ISSN=2297-055X

ABSTRACT=Background: Circulating miRNAs have been found to have different expressions in different phases of acute myocardial infarction. The profiles of plasma exosome-miRNAs in patients with ST-segment elevation myocardial infarction (STEMI) at 3-6 months post-infarction are unknown. 
Objective: The aim of the present study was to assess profiles of plasma exosome-miRNAs in patients with STEMI in comparison with healthy volunteers and to select specific exosome-miRNAs related to pathophysiological changes post-STEMI.
Methods: Plasma and echocardiography parameters were collected from 30 patients 3-6 months after STEMI and 30 healthy volunteers. Plasma exosome-miRNAs were assessed by using high-throughput sequence (Illumina Hiseq 2500) and profile of the plasma exosome-miRNAs was established in 10 patients and 6 healthy volunteers. The specific exosome-miRNAs related to heart diseases were selected according to Target Scan  Database. The specificity of the selected exosome-miRNAs was evaluated in an additional 20 post-STEMI patients and 24 healthy volunteers by using qPCR. Left ventricular remodeling (LVR) was defined using the European Association of Cardiovascular Imaging criteria according to echo-cardiography examination. Correlations between expression of the specific miRNAs and echocardiography parameters of LVR were assessed using Spearman correlation analysis.
Results: Twenty eight up-regulated miRNAs and 49 down-regulated miRNAs were found in patients 3-6 months after STEMI (p < 0.01) in comparison with the healthy. The two least expressed and heart-related exosome miRNAs were hsa-miR-181a-3p (0.64-fold, p < 0.01) and hsa-miR-874-3p (0.50-fold, p < 0.01) which were further confirmed by using qPCR and demonstrated significant specificity in another 20 post-STEMI patients comparing to 24 healthy volunteers (AUC = 0.68, p < 0.05; AUC = 0.74, p < 0.05) . Expression of miR-181a-3p was upregulated in patients without LVR compared to those patients with LVR.
Conclusion: Circulating exosome miR-874-3p and miR-181a-3p were down-regulated in patients with STEMI post-infarction. Exosome hsa-miR-181a-3p might play a potential role in development of LVR in post-STEMI patients.

Keywords: Exosome, miRNA, Myocardium remodeling, Post infarction, STEMI