AUTHOR=Jiang Chunhua , Moorthy Balaji T. , Patel Devang M. , Kumar Akhilesh , Morgan William M. , Alfonso Belkis , Huang Jingyu , Lampidis Theodore J. , Isom Daniel G. , Barrientos Antoni , Fontanesi Flavia , Zhang Fangliang 

TITLE=Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 8 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.603688

DOI=10.3389/fcell.2020.603688

ISSN=2296-634X

ABSTRACT=Arginyltransferase 1 (ATE1) is an evolutionary-conserved eukaryotic protein that localizes to the cytosol and nucleus. It is the only known enzyme in metazoans and fungi that catalyzes posttranslational arginylation. Lack of arginylation has been linked to an array of human disorders, including cancer, by altering the response to stress and the regulation of metabolism and apoptosis. Although mitochondria play relevant roles in these processes in health and disease, a causal relationship between ATE1 activity and mitochondrial biology has yet to be established. Here, we report a phylogenetic analysis that traces the roots of ATE1 to alpha-proteobacteria, the mitochondrion microbial ancestor.  We then demonstrate that a small fraction of ATE1 localizes within mitochondria. Furthermore, the absence of ATE1 influences the composition and function of respiratory complexes in mouse cells. Specifically, ATE1-KO mouse embryonic fibroblasts have decreased mitochondrial respiration owing to severely lowered levels of mitochondrial respiratory chain complex II, which leads to the accumulation of succinate and contributes to an increase in glycolysis. Taken together, our findings establish a novel pathway that regulates mitochondrial function and its connection to glycolysis. We believe these findings may explain many Ate1-dependent effects in various disease conditions, including cancer and aging, in which metabolic shifts are part of the pathogenic or deleterious underlying mechanism.