AUTHOR=Lu Guo-dong , Cheng Peng , Liu Ting , Wang Zhong 

TITLE=BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 8 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.608521

DOI=10.3389/fcell.2020.608521

ISSN=2296-634X

ABSTRACT=Angiogenesis and osteogenesis are tightly coupled during bone modeling and remodeling processes. Here we reported that bone marrow mesenchymal stem cells (BMSCs) derived exosomal miR-29a promote angiogenesis and osteogenesis in vitro and in vivo. BMSCs-Exos can be taken up by human umbilical vein endothelial cells (HUVECs) and promote proliferation, migration and tube formation of HUVECs. MiRNA-29a level was high in BMSCs-Exos and can be transported into HUVECs to regulate angiogenesis. VASH1 was identified as a direct target of miR-29a mediating the effects of BMSCs derived exosomal miR-29a on angiogenesis. More interestingly, miR29a-loaded exosomes from engineered BMSCs (miR-29a-loaded BMSCs-Exos) showed robust ability of promoting angiogenesis and osteogenesis in vivo. Taken together, these findings suggest that BMSCs derived exosomal miR-29a regulate angiogenesis and osteogenesis and miR-29a-loaded BMSCs-Exos may serve as a potential therapeutic target for osteoporosis.