AUTHOR=Brindo da Cruz Isabele Camargo , Velosa Ana Paula Pereira , Carrasco Solange , dos Santos Filho Antonio , Tomaz de Miranda Jurandir , Pompeu Eduardo , Fernandes Tiago Lazzaretti , Bueno Daniela Franco , Fanelli Camila , Goldenstein-Schainberg Cláudia , Fabro Alexandre Todorovic , Fuller Ricardo , Silva Pedro Leme , Capelozzi Vera Luiza , Teodoro Walcy Rosolia 

TITLE=Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.606890

DOI=10.3389/fcell.2021.606890

ISSN=2296-634X

ABSTRACT=Collagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induces significantly increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). In vitro, the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes, col2a1 and acan was significantly up-regulated and poul5fl down-regulated post-ADSC/Col V treatment. In vivo analyses revealed that post-ADSC/Col V treatment, rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H&E) and Safranin-O/fast-green. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein was significantly increased, and apoptosis Fas-ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage, hence ADSC/Col V may be a promising therapeutic target for OA treatment.